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The map label for this gene is leuB

Identifier: 54026191

GI number: 54026191

Start: 4370177

End: 4371184

Strand: Reverse

Name: leuB

Synonym: nfa42200

Alternate gene names: 54026191

Gene position: 4371184-4370177 (Counterclockwise)

Preceding gene: 54026192

Following gene: 54026190

Centisome position: 72.6

GC content: 70.73

Gene sequence:

>1008_bases
ATGAAGCTTGCGGTCATCCCCGGTGACGGCATCGGTCCCGAGGTCATCGCCGAGGCGCTGAAGGTGCTCGACGTGGTCGT
GCCCGGCGTCGAGAAGACCGAATACGACCTGGGCGCCAAGCGCTATCACGCGACCGGCGAGATCCTGCCCGACTCGGTGC
TGCCGGAACTGCGCGAGCACGACGCGATCCTGCTCGGCGCGATTGGTGACCCGTCGGTGCCCAGCGGCGTGCTCGAGCGC
GGCCTGCTGCTGCGCACCCGTTTCGAGCTCGACCACCACGTGAACCTGCGGCCGTCGAGGCTGTTCACCGGCGTGCGGAG
CCCACTGGCCGGTGCACCCGACATCGATTTCGTCGTCGTGCGCGAGGGCACCGAGGGCCCCTACACCGGCACCGGCGGCG
CGATCCGGGTGCGGACGCCGCACGAGGTCGCCACCGAGGTCAGCACCAACACCCGCTTCGGCATCGAGCGGGTCGTGCGC
TACGCCTTCGCCAAGGCGCAGGCCAGGCGCAAGCACCTCACACTGGTGCACAAGACCAATGTGCTGACCTTCGCCGGGTC
GCTGTGGCAGCGCACCGTCGACGAGGTGGGCGCGGAGTTCCCCGAGGTCACGGTCGCCTACCAGCACATCGACGCCGCCA
CCATCCACATGGTCACCGACCCGGGCCGCTTCGACGTGATCGTCACCGACAACCTGTTCGGCGACATCATCACCGACCTC
GCGGCCGCGGTGAGCGGCGGCATCGGCCTGGCGGCCAGCGGCAACATCGACGCCTCCGGCACCAACCCGAGCATGTTCGA
GCCGGTGCACGGCAGCGCGCCCGACATCGCGGGCCAGTCGAAGGCCGACCCGACCGCCGCCATCCTGTCGGTCTCGCTGC
TGCTGAACCACCTCGGCGACACCGAGGCCGCCGCGCGGATCGACGCCGCGGTCGCCAAGGACCTGGCCGCCCGCTCGGGC
ACCGCCTCCACCGTCGAGATCGGCGACCGGATCGCCGCGGCGGTCTGA

Upstream 100 bases:

>100_bases
GCCAAGATCGCCCTGGTCGACCTGTTCTGATCGAAACGACGTCGCCGCGCGCCGCCACCGCACCCCGGTGGCGGCGCGTG
GGGCATGACGAGGAGTTGTC

Downstream 100 bases:

>100_bases
ACCGGTCATGACACGGGCCCCGCATCCGCACCGGATGCGGGGCCCGTCCTGTCTCGTTCGGGCGGCGCCCAGGTGGGTAT
CCCGAACGCACGGTATCGCC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKLAVIPGDGIGPEVIAEALKVLDVVVPGVEKTEYDLGAKRYHATGEILPDSVLPELREHDAILLGAIGDPSVPSGVLER
GLLLRTRFELDHHVNLRPSRLFTGVRSPLAGAPDIDFVVVREGTEGPYTGTGGAIRVRTPHEVATEVSTNTRFGIERVVR
YAFAKAQARRKHLTLVHKTNVLTFAGSLWQRTVDEVGAEFPEVTVAYQHIDAATIHMVTDPGRFDVIVTDNLFGDIITDL
AAAVSGGIGLAASGNIDASGTNPSMFEPVHGSAPDIAGQSKADPTAAILSVSLLLNHLGDTEAAARIDAAVAKDLAARSG
TASTVEIGDRIAAAV

Sequences:

>Translated_335_residues
MKLAVIPGDGIGPEVIAEALKVLDVVVPGVEKTEYDLGAKRYHATGEILPDSVLPELREHDAILLGAIGDPSVPSGVLER
GLLLRTRFELDHHVNLRPSRLFTGVRSPLAGAPDIDFVVVREGTEGPYTGTGGAIRVRTPHEVATEVSTNTRFGIERVVR
YAFAKAQARRKHLTLVHKTNVLTFAGSLWQRTVDEVGAEFPEVTVAYQHIDAATIHMVTDPGRFDVIVTDNLFGDIITDL
AAAVSGGIGLAASGNIDASGTNPSMFEPVHGSAPDIAGQSKADPTAAILSVSLLLNHLGDTEAAARIDAAVAKDLAARSG
TASTVEIGDRIAAAV
>Mature_335_residues
MKLAVIPGDGIGPEVIAEALKVLDVVVPGVEKTEYDLGAKRYHATGEILPDSVLPELREHDAILLGAIGDPSVPSGVLER
GLLLRTRFELDHHVNLRPSRLFTGVRSPLAGAPDIDFVVVREGTEGPYTGTGGAIRVRTPHEVATEVSTNTRFGIERVVR
YAFAKAQARRKHLTLVHKTNVLTFAGSLWQRTVDEVGAEFPEVTVAYQHIDAATIHMVTDPGRFDVIVTDNLFGDIITDL
AAAVSGGIGLAASGNIDASGTNPSMFEPVHGSAPDIAGQSKADPTAAILSVSLLLNHLGDTEAAARIDAAVAKDLAARSG
TASTVEIGDRIAAAV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=327, Percent_Identity=33.6391437308869, Blast_Score=184, Evalue=8e-47,
Organism=Homo sapiens, GI4758582, Length=316, Percent_Identity=30.6962025316456, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI28178838, Length=316, Percent_Identity=30.6962025316456, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI28178816, Length=298, Percent_Identity=29.8657718120805, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI28178821, Length=300, Percent_Identity=29.6666666666667, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI28178819, Length=151, Percent_Identity=33.7748344370861, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=41.8539325842697, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=38.2022471910112, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1787381, Length=365, Percent_Identity=32.0547945205479, Blast_Score=140, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI71986051, Length=332, Percent_Identity=34.0361445783133, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17550882, Length=346, Percent_Identity=31.7919075144509, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=318, Percent_Identity=29.874213836478, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=326, Percent_Identity=29.4478527607362, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324709, Length=319, Percent_Identity=35.1097178683386, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=35.5555555555556, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324291, Length=342, Percent_Identity=33.6257309941521, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322097, Length=351, Percent_Identity=33.6182336182336, Blast_Score=145, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24643270, Length=334, Percent_Identity=33.8323353293413, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24643268, Length=334, Percent_Identity=33.8323353293413, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24661184, Length=339, Percent_Identity=33.0383480825959, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI161078637, Length=342, Percent_Identity=28.0701754385965, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078633, Length=342, Percent_Identity=28.0701754385965, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24650122, Length=342, Percent_Identity=28.0701754385965, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078635, Length=342, Percent_Identity=28.0701754385965, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078639, Length=340, Percent_Identity=28.2352941176471, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281362242, Length=332, Percent_Identity=27.710843373494, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648872, Length=332, Percent_Identity=27.710843373494, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20130355, Length=315, Percent_Identity=25.0793650793651, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEU3_NOCFA (Q5YRX2)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120433.1
- ProteinModelPortal:   Q5YRX2
- SMR:   Q5YRX2
- GeneID:   3111207
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa42200
- NMPDR:   fig|247156.1.peg.4069
- HOGENOM:   HBG518924
- OMA:   AATIFMV
- ProtClustDB:   PRK03437
- BioCyc:   NFAR247156:NFA42200-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01035
- InterPro:   IPR019818
- InterPro:   IPR001804
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 35247; Mature: 35247

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 87-87 BINDING 97-97 BINDING 121-121 BINDING 211-211

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVIPGDGIGPEVIAEALKVLDVVVPGVEKTEYDLGAKRYHATGEILPDSVLPELREH
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCHHEECCCCCCCHHHHHHHHHC
DAILLGAIGDPSVPSGVLERGLLLRTRFELDHHVNLRPSRLFTGVRSPLAGAPDIDFVVV
CEEEEEECCCCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEE
REGTEGPYTGTGGAIRVRTPHEVATEVSTNTRFGIERVVRYAFAKAQARRKHLTLVHKTN
ECCCCCCCCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECC
VLTFAGSLWQRTVDEVGAEFPEVTVAYQHIDAATIHMVTDPGRFDVIVTDNLFGDIITDL
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEEECCCEEEEEEECCHHHHHHHHH
AAAVSGGIGLAASGNIDASGTNPSMFEPVHGSAPDIAGQSKADPTAAILSVSLLLNHLGD
HHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
TEAAARIDAAVAKDLAARSGTASTVEIGDRIAAAV
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCC
>Mature Secondary Structure
MKLAVIPGDGIGPEVIAEALKVLDVVVPGVEKTEYDLGAKRYHATGEILPDSVLPELREH
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCHHEECCCCCCCHHHHHHHHHC
DAILLGAIGDPSVPSGVLERGLLLRTRFELDHHVNLRPSRLFTGVRSPLAGAPDIDFVVV
CEEEEEECCCCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEE
REGTEGPYTGTGGAIRVRTPHEVATEVSTNTRFGIERVVRYAFAKAQARRKHLTLVHKTN
ECCCCCCCCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECC
VLTFAGSLWQRTVDEVGAEFPEVTVAYQHIDAATIHMVTDPGRFDVIVTDNLFGDIITDL
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEEECCCEEEEEEECCHHHHHHHHH
AAAVSGGIGLAASGNIDASGTNPSMFEPVHGSAPDIAGQSKADPTAAILSVSLLLNHLGD
HHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
TEAAARIDAAVAKDLAARSGTASTVEIGDRIAAAV
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA