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The map label for this gene is leuC

Identifier: 54026183

GI number: 54026183

Start: 4362121

End: 4363545

Strand: Reverse

Name: leuC

Synonym: nfa42120

Alternate gene names: 54026183

Gene position: 4363545-4362121 (Counterclockwise)

Preceding gene: 54026187

Following gene: 54026182

Centisome position: 72.47

GC content: 70.74

Gene sequence:

>1425_bases
ATGGCCGACCGCCCACGTACCCTGGCCGAGAAGGTCTGGGAAGAGCACGTCGTCGCCCGGGGTGAGGGCGAGGGCGCCTC
GCGCGAGCCGGATCTGATCTACATCGACCTGCACCTCGTGCACGAGGTCACCAGCCCGCAGGCCTTCGACGGCCTGCGCG
CCGCGGGCCGCCCGGTCCGCCGTCCCGACCTCACCATCGCCACCGAGGACCACAACGTCCCCACCGTCGACATCGACAAG
CCGATCGCCGACCCGATCTCACGCACGCAGGTCGAGACGCTGCGCCGCAACTGCGCCGAATTCGGCATCCGGCTGCACCC
GATGGGGGACCTGGAACAGGGCATCGTGCACGTGGTCGGCCCGCAGCTCGGCCTGACCCAGCCCGGCATGACCGTGGTCT
GCGGTGACAGCCACACCTCCACCCACGGCGCCTTCGGCGCGCTGGCGATGGGTATCGGCACCAGCGAGGTCGAGCACGTG
CTGGCCACGCAGACCTTGTCGCTGCGCCCGTTCAAGACCATGGCGATCAACATCGACGGGCAGCTGCCGCCCGGGGTCAC
CAGCAAGGACGTCATCCTGGCCGTGATCGCCCAGATCGGCACCGGTGGCGGCCAGGGCTATGTGCTCGAGTACCGCGGCG
AGGCCGTGCGCGCCATGTCGATGGAGGCGCGGATGACGATGTGCAACATGTCCATCGAGGCGGGTGCGCGAGCCGGCATG
ATCGCCCCCGACGAGGTGACCTACGAGTTCCTGAAGGGGCGTCCGCACGCGCCGAAGGGTGCCGACTGGGACGCCGCGGT
GGCCGCGTGGGACGCGCTGCGGACCGACGAGGGCGCTGTTTTCGACGCCGAGGTGCACATCGACGCGAGCTCGCTGAGCC
CGTTCGTCACCTGGGGCACGAACCCGGGCCAGGGTGCGCCGCTGAGCGCGACCGTGCCCGATCCGGCCGATTTCGCCGAC
GAGACCGCCCGCGAGGCCGCCGCGAAAGCGCTGCGGTACATGGATTTGACCCCGGGCACGCCGCTGCGCGAAGTCGCCGT
CGACACCGTTTTCGTCGGCTCGTGCACCAACGGCCGGATCGAGGATTTGCGGGCGGCGGCCGAAATCCTGAAGGGCCGCC
GGGTTGCCGAGGGCGTGCGAATGCTGGTCGTACCGGGCTCGATGCGGGTGCGCGCGCAGGCGGAAAAGGAGGGGCTCGGC
GAGATTTTCACCGCCGCGGGCGCCGAATGGCGGCAGGCGGGCTGCTCGATGTGCCTGGGCATGAATCCCGACCAGCTCGA
GCCCGGCCAACGCTGCGCCTCCACCTCGAACCGCAACTTCGAAGGCAGGCAGGGCAAGGGCGGCCGCACCCATCTGGTCT
CGCCGCTGGTGGCGGCCGCGACCGCGGTGCGCGGGACCCTGTCCTCACCGGCCGACCTGGACTGA

Upstream 100 bases:

>100_bases
GGGAGATGCGGCTTCCGGCCGGGACGCGCAATCCCAGAAGTCGAACCGCGGCGGCACAGCCCGCCGTCGCGGCCGCAGCC
CGTTCGAAAGGTGATCGAGA

Downstream 100 bases:

>100_bases
CGTCCACCCAGACACCCGAACCGACCCAGGAGAATCCCGATGGAAGCCTTCACCGTGCACAAGGGAATCGGCGTGCCGCT
GCGCCGGTCCAATGTCGACA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MADRPRTLAEKVWEEHVVARGEGEGASREPDLIYIDLHLVHEVTSPQAFDGLRAAGRPVRRPDLTIATEDHNVPTVDIDK
PIADPISRTQVETLRRNCAEFGIRLHPMGDLEQGIVHVVGPQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVEHV
LATQTLSLRPFKTMAINIDGQLPPGVTSKDVILAVIAQIGTGGGQGYVLEYRGEAVRAMSMEARMTMCNMSIEAGARAGM
IAPDEVTYEFLKGRPHAPKGADWDAAVAAWDALRTDEGAVFDAEVHIDASSLSPFVTWGTNPGQGAPLSATVPDPADFAD
ETAREAAAKALRYMDLTPGTPLREVAVDTVFVGSCTNGRIEDLRAAAEILKGRRVAEGVRMLVVPGSMRVRAQAEKEGLG
EIFTAAGAEWRQAGCSMCLGMNPDQLEPGQRCASTSNRNFEGRQGKGGRTHLVSPLVAAATAVRGTLSSPADLD

Sequences:

>Translated_474_residues
MADRPRTLAEKVWEEHVVARGEGEGASREPDLIYIDLHLVHEVTSPQAFDGLRAAGRPVRRPDLTIATEDHNVPTVDIDK
PIADPISRTQVETLRRNCAEFGIRLHPMGDLEQGIVHVVGPQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVEHV
LATQTLSLRPFKTMAINIDGQLPPGVTSKDVILAVIAQIGTGGGQGYVLEYRGEAVRAMSMEARMTMCNMSIEAGARAGM
IAPDEVTYEFLKGRPHAPKGADWDAAVAAWDALRTDEGAVFDAEVHIDASSLSPFVTWGTNPGQGAPLSATVPDPADFAD
ETAREAAAKALRYMDLTPGTPLREVAVDTVFVGSCTNGRIEDLRAAAEILKGRRVAEGVRMLVVPGSMRVRAQAEKEGLG
EIFTAAGAEWRQAGCSMCLGMNPDQLEPGQRCASTSNRNFEGRQGKGGRTHLVSPLVAAATAVRGTLSSPADLD
>Mature_473_residues
ADRPRTLAEKVWEEHVVARGEGEGASREPDLIYIDLHLVHEVTSPQAFDGLRAAGRPVRRPDLTIATEDHNVPTVDIDKP
IADPISRTQVETLRRNCAEFGIRLHPMGDLEQGIVHVVGPQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVEHVL
ATQTLSLRPFKTMAINIDGQLPPGVTSKDVILAVIAQIGTGGGQGYVLEYRGEAVRAMSMEARMTMCNMSIEAGARAGMI
APDEVTYEFLKGRPHAPKGADWDAAVAAWDALRTDEGAVFDAEVHIDASSLSPFVTWGTNPGQGAPLSATVPDPADFADE
TAREAAAKALRYMDLTPGTPLREVAVDTVFVGSCTNGRIEDLRAAAEILKGRRVAEGVRMLVVPGSMRVRAQAEKEGLGE
IFTAAGAEWRQAGCSMCLGMNPDQLEPGQRCASTSNRNFEGRQGKGGRTHLVSPLVAAATAVRGTLSSPADLD

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=387, Percent_Identity=27.390180878553, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI8659555, Length=465, Percent_Identity=25.1612903225806, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=62.4190064794816, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI87081781, Length=360, Percent_Identity=26.3888888888889, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787531, Length=363, Percent_Identity=25.3443526170799, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI2367097, Length=138, Percent_Identity=34.0579710144928, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI32564738, Length=365, Percent_Identity=30.4109589041096, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=30.4109589041096, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=319, Percent_Identity=30.4075235109718, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=395, Percent_Identity=25.0632911392405, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321429, Length=458, Percent_Identity=58.5152838427948, Blast_Score=555, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6320440, Length=491, Percent_Identity=25.8655804480652, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=403, Percent_Identity=27.0471464019851, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=481, Percent_Identity=26.1954261954262, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161076999, Length=393, Percent_Identity=26.972010178117, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281365315, Length=399, Percent_Identity=27.0676691729323, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17864292, Length=399, Percent_Identity=27.0676691729323, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI28571643, Length=488, Percent_Identity=26.4344262295082, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24645686, Length=367, Percent_Identity=27.2479564032698, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17137564, Length=368, Percent_Identity=25.8152173913043, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_NOCFA (Q5YRY0)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120425.1
- ProteinModelPortal:   Q5YRY0
- GeneID:   3111208
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa42120
- NMPDR:   fig|247156.1.peg.4061
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   NFAR247156:NFA42120-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50366; Mature: 50234

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRPRTLAEKVWEEHVVARGEGEGASREPDLIYIDLHLVHEVTSPQAFDGLRAAGRPVR
CCCCCHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCCC
RPDLTIATEDHNVPTVDIDKPIADPISRTQVETLRRNCAEFGIRLHPMGDLEQGIVHVVG
CCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCHHHCCEEEEC
PQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLSLRPFKTMAINIDG
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEECC
QLPPGVTSKDVILAVIAQIGTGGGQGYVLEYRGEAVRAMSMEARMTMCNMSIEAGARAGM
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHCCCCCCC
IAPDEVTYEFLKGRPHAPKGADWDAAVAAWDALRTDEGAVFDAEVHIDASSLSPFVTWGT
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEHHHCCCEEEECC
NPGQGAPLSATVPDPADFADETAREAAAKALRYMDLTPGTPLREVAVDTVFVGSCTNGRI
CCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHEEEEEEECCCCCHH
EDLRAAAEILKGRRVAEGVRMLVVPGSMRVRAQAEKEGLGEIFTAAGAEWRQAGCSMCLG
HHHHHHHHHHHCCHHHCCCEEEEECCCCEEEEHHHHCCHHHHHHHCCCHHHHHCCCEEEC
MNPDQLEPGQRCASTSNRNFEGRQGKGGRTHLVSPLVAAATAVRGTLSSPADLD
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ADRPRTLAEKVWEEHVVARGEGEGASREPDLIYIDLHLVHEVTSPQAFDGLRAAGRPVR
CCCCHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCCC
RPDLTIATEDHNVPTVDIDKPIADPISRTQVETLRRNCAEFGIRLHPMGDLEQGIVHVVG
CCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCHHHCCEEEEC
PQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLSLRPFKTMAINIDG
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEECC
QLPPGVTSKDVILAVIAQIGTGGGQGYVLEYRGEAVRAMSMEARMTMCNMSIEAGARAGM
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHCCCCCCC
IAPDEVTYEFLKGRPHAPKGADWDAAVAAWDALRTDEGAVFDAEVHIDASSLSPFVTWGT
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEHHHCCCEEEECC
NPGQGAPLSATVPDPADFADETAREAAAKALRYMDLTPGTPLREVAVDTVFVGSCTNGRI
CCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHEEEEEEECCCCCHH
EDLRAAAEILKGRRVAEGVRMLVVPGSMRVRAQAEKEGLGEIFTAAGAEWRQAGCSMCLG
HHHHHHHHHHHCCHHHCCCEEEEECCCCEEEEHHHHCCHHHHHHHCCCHHHHHCCCEEEC
MNPDQLEPGQRCASTSNRNFEGRQGKGGRTHLVSPLVAAATAVRGTLSSPADLD
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA