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The map label for this gene is gltX

Identifier: 54026187

GI number: 54026187

Start: 4365798

End: 4367267

Strand: Reverse

Name: gltX

Synonym: nfa42160

Alternate gene names: 54026187

Gene position: 4367267-4365798 (Counterclockwise)

Preceding gene: 54026188

Following gene: 54026183

Centisome position: 72.53

GC content: 68.23

Gene sequence:

>1470_bases
ATGACAGAAGTACGGGTCCGTTTCTGCCCGTCGCCGACCGGGACGCCGCACGTCGGCCTGATCCGCACGGCCCTGTTCAA
CTGGGCCTACGCCCGCCACACCGGCGGCACCTTCGTCTTCCGCATCGAAGACACCGACGCCGCACGCGATTCCGAGGAGT
CCTACCACGCGATCCTCGACGCGCTGCGCTGGCTCGGGCTCACCTGGGACGAGGGGCCCGAGGTCGGCGGCCCGTACGGT
CCCTACCGCCAATCGCAGCGGCGGGAACTGTATCTGGACGTGGTGCGGCGGCTGCTCGAGGCCGGCGAGGCCTACGAATC
GTTCTCCACCCCGGAGGAGGTCGAGGCCCGCCATCGTGCCGCGGGCCGCGATCCGAAGCTCGGCTACGACAATTACGACC
GCGATCTCACCCCCGAGGCGATCGAGGCCTACCGGGCCGCGGGCCGGCCCGCCGTGGTGCGCCTGCGGATGCCCGACGAG
GACCTCACGTGGACCGACCTGGTGCGCGGTGAGACCACCTTCAAGGCGGGCACGGTGCCCGACTTCGCGCTCACCCGCGG
TAATGGTGATCCGCTCTACACGCTGGTCAACCCGGTCGACGACGCCTCGATGAAGATCACCCACGTACTGCGCGGCGAGG
ATCTGCTCTCCTCCACGCCGCGGCAGCTCGCGCTCTACGCGGCATTGCGCCGGATCGGGGTGGCCGACTTCACCCCGGAG
TTCGGTCACCTGCCATTCGTGATGGGGCAGGGCAACAAGAAGTTGTCCAAGCGCGATCCGGAGTCCAACCTGTTCGCTCA
CCGCGACCGCGGATTCATCCCGGAAGGTTTGCTGAATTACCTCGCATTGCTCGGCTGGAGTCTCGCCGATGACCGCGACG
TGTTCTCCATGTCCGAGATGGTGGCGGCGTTCGACATTTCGAAAGTGAATTCGAATCCCGCCCGATTCGACCAGAAGAAG
GCCGACGCGCTCAACGCCGAGCACATCCGATTGCTCGAACCGGGTGATTTCGCCCACCGCTTGCGGCAGTTCCTCACCGA
ACACGGCCACATCGGCGCGGACGTGGACGAGAAAGTGTTCGCCGCGGCCGCCGAATTGGTGCAGACGCGCATCGTGGTGC
TCGGCGACGCCTGGGATCTGCTGCGATTCCTGTTCGCGCCGGCCGACGAATTCACCGTCGACGAGGCCGCGGCCGCCAAG
AACCTCGGCGCCGACGCCGTGCCGGTGCTCGAGGCCGCCATCGCCGCGCTGGAGCCGCTGCCCGGGTGGGCCACCCCGGT
CATCGAGGACGCCCTCAAAACGGCGCTGATCGACGATCTGGGGCTCAAACCACGCAAGGCCTTCGCGCCGGTGCGGGTGG
CGGTCACCGGCTCGCACATCAGCCCGCCGCTGTACGAGTCGCTGGAGCTGCTCGGGCGCGAGCTCACCCTGGCGCGGCTG
CGCTCCGGGCTCGAGTGGGCGCGGCAGTAG

Upstream 100 bases:

>100_bases
GTCGGTCCGATGCAGGTCGGACAGCAGGTGTCGGTCACCGTCGAGGGAATCGGCACCCTGACCAATCCCGTTGCCGCCAA
ACGCTGATCGAGAGAGAGTC

Downstream 100 bases:

>100_bases
TCGAAAGCGGCCCAAAACAGGGCCTTGACCAGGCGATTTGTAGTTTACCCGCCGAGGTTTGGTAATCTCTTCCTCGGCCG
GAAACGCGGCCCCGAGTCAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTEVRVRFCPSPTGTPHVGLIRTALFNWAYARHTGGTFVFRIEDTDAARDSEESYHAILDALRWLGLTWDEGPEVGGPYG
PYRQSQRRELYLDVVRRLLEAGEAYESFSTPEEVEARHRAAGRDPKLGYDNYDRDLTPEAIEAYRAAGRPAVVRLRMPDE
DLTWTDLVRGETTFKAGTVPDFALTRGNGDPLYTLVNPVDDASMKITHVLRGEDLLSSTPRQLALYAALRRIGVADFTPE
FGHLPFVMGQGNKKLSKRDPESNLFAHRDRGFIPEGLLNYLALLGWSLADDRDVFSMSEMVAAFDISKVNSNPARFDQKK
ADALNAEHIRLLEPGDFAHRLRQFLTEHGHIGADVDEKVFAAAAELVQTRIVVLGDAWDLLRFLFAPADEFTVDEAAAAK
NLGADAVPVLEAAIAALEPLPGWATPVIEDALKTALIDDLGLKPRKAFAPVRVAVTGSHISPPLYESLELLGRELTLARL
RSGLEWARQ

Sequences:

>Translated_489_residues
MTEVRVRFCPSPTGTPHVGLIRTALFNWAYARHTGGTFVFRIEDTDAARDSEESYHAILDALRWLGLTWDEGPEVGGPYG
PYRQSQRRELYLDVVRRLLEAGEAYESFSTPEEVEARHRAAGRDPKLGYDNYDRDLTPEAIEAYRAAGRPAVVRLRMPDE
DLTWTDLVRGETTFKAGTVPDFALTRGNGDPLYTLVNPVDDASMKITHVLRGEDLLSSTPRQLALYAALRRIGVADFTPE
FGHLPFVMGQGNKKLSKRDPESNLFAHRDRGFIPEGLLNYLALLGWSLADDRDVFSMSEMVAAFDISKVNSNPARFDQKK
ADALNAEHIRLLEPGDFAHRLRQFLTEHGHIGADVDEKVFAAAAELVQTRIVVLGDAWDLLRFLFAPADEFTVDEAAAAK
NLGADAVPVLEAAIAALEPLPGWATPVIEDALKTALIDDLGLKPRKAFAPVRVAVTGSHISPPLYESLELLGRELTLARL
RSGLEWARQ
>Mature_488_residues
TEVRVRFCPSPTGTPHVGLIRTALFNWAYARHTGGTFVFRIEDTDAARDSEESYHAILDALRWLGLTWDEGPEVGGPYGP
YRQSQRRELYLDVVRRLLEAGEAYESFSTPEEVEARHRAAGRDPKLGYDNYDRDLTPEAIEAYRAAGRPAVVRLRMPDED
LTWTDLVRGETTFKAGTVPDFALTRGNGDPLYTLVNPVDDASMKITHVLRGEDLLSSTPRQLALYAALRRIGVADFTPEF
GHLPFVMGQGNKKLSKRDPESNLFAHRDRGFIPEGLLNYLALLGWSLADDRDVFSMSEMVAAFDISKVNSNPARFDQKKA
DALNAEHIRLLEPGDFAHRLRQFLTEHGHIGADVDEKVFAAAAELVQTRIVVLGDAWDLLRFLFAPADEFTVDEAAAAKN
LGADAVPVLEAAIAALEPLPGWATPVIEDALKTALIDDLGLKPRKAFAPVRVAVTGSHISPPLYESLELLGRELTLARLR
SGLEWARQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=339, Percent_Identity=35.693215339233, Blast_Score=205, Evalue=9e-53,
Organism=Escherichia coli, GI1788743, Length=489, Percent_Identity=34.1513292433538, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI226510925, Length=259, Percent_Identity=30.5019305019305, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71993394, Length=491, Percent_Identity=29.7352342158859, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=505, Percent_Identity=30.4950495049505, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24665321, Length=500, Percent_Identity=32.2, Blast_Score=231, Evalue=6e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_NOCFA (Q5YRX6)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120429.1
- ProteinModelPortal:   Q5YRX6
- SMR:   Q5YRX6
- GeneID:   3109603
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa42160
- NMPDR:   fig|247156.1.peg.4065
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   NFAR247156:NFA42160-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54252; Mature: 54121

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 257-257

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVRVRFCPSPTGTPHVGLIRTALFNWAYARHTGGTFVFRIEDTDAARDSEESYHAILD
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHH
ALRWLGLTWDEGPEVGGPYGPYRQSQRRELYLDVVRRLLEAGEAYESFSTPEEVEARHRA
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHH
AGRDPKLGYDNYDRDLTPEAIEAYRAAGRPAVVRLRMPDEDLTWTDLVRGETTFKAGTVP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCCC
DFALTRGNGDPLYTLVNPVDDASMKITHVLRGEDLLSSTPRQLALYAALRRIGVADFTPE
CCEEECCCCCEEEEEECCCCCCCEEEHHHHCCCHHHHCCHHHHHHHHHHHHCCCCCCCCC
FGHLPFVMGQGNKKLSKRDPESNLFAHRDRGFIPEGLLNYLALLGWSLADDRDVFSMSEM
CCCCCEEECCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHH
VAAFDISKVNSNPARFDQKKADALNAEHIRLLEPGDFAHRLRQFLTEHGHIGADVDEKVF
HHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHH
AAAAELVQTRIVVLGDAWDLLRFLFAPADEFTVDEAAAAKNLGADAVPVLEAAIAALEPL
HHHHHHHHHHEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCC
PGWATPVIEDALKTALIDDLGLKPRKAFAPVRVAVTGSHISPPLYESLELLGRELTLARL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
RSGLEWARQ
HHHHHHHCC
>Mature Secondary Structure 
TEVRVRFCPSPTGTPHVGLIRTALFNWAYARHTGGTFVFRIEDTDAARDSEESYHAILD
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHH
ALRWLGLTWDEGPEVGGPYGPYRQSQRRELYLDVVRRLLEAGEAYESFSTPEEVEARHRA
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHH
AGRDPKLGYDNYDRDLTPEAIEAYRAAGRPAVVRLRMPDEDLTWTDLVRGETTFKAGTVP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCCC
DFALTRGNGDPLYTLVNPVDDASMKITHVLRGEDLLSSTPRQLALYAALRRIGVADFTPE
CCEEECCCCCEEEEEECCCCCCCEEEHHHHCCCHHHHCCHHHHHHHHHHHHCCCCCCCCC
FGHLPFVMGQGNKKLSKRDPESNLFAHRDRGFIPEGLLNYLALLGWSLADDRDVFSMSEM
CCCCCEEECCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHH
VAAFDISKVNSNPARFDQKKADALNAEHIRLLEPGDFAHRLRQFLTEHGHIGADVDEKVF
HHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHH
AAAAELVQTRIVVLGDAWDLLRFLFAPADEFTVDEAAAAKNLGADAVPVLEAAIAALEPL
HHHHHHHHHHEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCC
PGWATPVIEDALKTALIDDLGLKPRKAFAPVRVAVTGSHISPPLYESLELLGRELTLARL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
RSGLEWARQ
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA