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The map label for this gene is leuD

Identifier: 54026182

GI number: 54026182

Start: 4361473

End: 4362081

Strand: Reverse

Name: leuD

Synonym: nfa42110

Alternate gene names: 54026182

Gene position: 4362081-4361473 (Counterclockwise)

Preceding gene: 54026183

Following gene: 54026181

Centisome position: 72.45

GC content: 63.38

Gene sequence:

>609_bases
ATGGAAGCCTTCACCGTGCACAAGGGAATCGGCGTGCCGCTGCGCCGGTCCAATGTCGACACCGACCAGATCATTCCGGC
CGTCTACCTCAAGCGGGTCACCCGCACCGGTTTCGAGGACGCGCTGTTCGCCGCATGGCGCTCCGATCCGGACTTCATTC
TGAACACCGAGCCCTTCAACCGCGGCACCGTGCTCGTCGCCGGACCGGATTTCGGTACCGGATCCTCGCGTGAGCACGCG
GTGTGGGCGCTGTCGGACTACGGCTTTCGAGTGGTCATCTCCTCCCGTTTCGCCGACATCTTCCGCGGCAATGCGGGCAA
GGGCGGCCTGCTGGCCGCGCAGATGTCACAGAACGATGTCGAAATGCTCTGGAAGATGATCGAGGAACAGCCCGGCCTCG
AATTGGTGGTGGACCTGCGCGACCGCACCGTCACCGCGGGAACCGTCGTGTTGCCGTTCGATATTGATGACTACACCCGG
TGGCGTCTGCTGGAAGGATTGGACGACATCGGTCTCACGCTGCGCCGCAGCGACGTCATCGCCGAGTTCGAAAAGGCAAG
GCCGACATGGAAGCCCACCACCCTCCCGGCGCCTATTTCGCAGGCGTAA

Upstream 100 bases:

>100_bases
GCTGGTGGCGGCCGCGACCGCGGTGCGCGGGACCCTGTCCTCACCGGCCGACCTGGACTGACGTCCACCCAGACACCCGA
ACCGACCCAGGAGAATCCCG

Downstream 100 bases:

>100_bases
TCATCCGGGAGTGATAGCTGGCGCCGATCAGGGGGCGGTAGCTGGACGTGTGCTAAACCACCATGAATTTTGGCGTGGCA
CATAGACTCTTGCCCAAAGT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MEAFTVHKGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDALFAAWRSDPDFILNTEPFNRGTVLVAGPDFGTGSSREHA
VWALSDYGFRVVISSRFADIFRGNAGKGGLLAAQMSQNDVEMLWKMIEEQPGLELVVDLRDRTVTAGTVVLPFDIDDYTR
WRLLEGLDDIGLTLRRSDVIAEFEKARPTWKPTTLPAPISQA

Sequences:

>Translated_202_residues
MEAFTVHKGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDALFAAWRSDPDFILNTEPFNRGTVLVAGPDFGTGSSREHA
VWALSDYGFRVVISSRFADIFRGNAGKGGLLAAQMSQNDVEMLWKMIEEQPGLELVVDLRDRTVTAGTVVLPFDIDDYTR
WRLLEGLDDIGLTLRRSDVIAEFEKARPTWKPTTLPAPISQA
>Mature_202_residues
MEAFTVHKGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDALFAAWRSDPDFILNTEPFNRGTVLVAGPDFGTGSSREHA
VWALSDYGFRVVISSRFADIFRGNAGKGGLLAAQMSQNDVEMLWKMIEEQPGLELVVDLRDRTVTAGTVVLPFDIDDYTR
WRLLEGLDDIGLTLRRSDVIAEFEKARPTWKPTTLPAPISQA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=45.1776649746193, Blast_Score=177, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321429, Length=201, Percent_Identity=39.3034825870647, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_NOCFA (Q5YRY1)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120424.1
- ProteinModelPortal:   Q5YRY1
- SMR:   Q5YRY1
- GeneID:   3111209
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa42110
- NMPDR:   fig|247156.1.peg.4060
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   NFAR247156:NFA42110-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22526; Mature: 22526

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAFTVHKGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDALFAAWRSDPDFILNTEPFN
CCCEEECCCCCCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCC
RGTVLVAGPDFGTGSSREHAVWALSDYGFRVVISSRFADIFRGNAGKGGLLAAQMSQNDV
CCEEEEECCCCCCCCCCCCEEEEECCCCEEEEEEHHHHHHHCCCCCCCCEEEEECCCCHH
EMLWKMIEEQPGLELVVDLRDRTVTAGTVVLPFDIDDYTRWRLLEGLDDIGLTLRRSDVI
HHHHHHHHHCCCCEEEEEECCCEEECCEEEEEEECCCHHHHHHHHCCHHHCEEEEHHHHH
AEFEKARPTWKPTTLPAPISQA
HHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEAFTVHKGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDALFAAWRSDPDFILNTEPFN
CCCEEECCCCCCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCC
RGTVLVAGPDFGTGSSREHAVWALSDYGFRVVISSRFADIFRGNAGKGGLLAAQMSQNDV
CCEEEEECCCCCCCCCCCCEEEEECCCCEEEEEEHHHHHHHCCCCCCCCEEEEECCCCHH
EMLWKMIEEQPGLELVVDLRDRTVTAGTVVLPFDIDDYTRWRLLEGLDDIGLTLRRSDVI
HHHHHHHHHCCCCEEEEEECCCEEECCEEEEEEECCCHHHHHHHHCCHHHCEEEEHHHHH
AEFEKARPTWKPTTLPAPISQA
HHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA