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The map label for this gene is miaA

Identifier: 54025801

GI number: 54025801

Start: 4050869

End: 4051810

Strand: Reverse

Name: miaA

Synonym: nfa38310

Alternate gene names: 54025801

Gene position: 4051810-4050869 (Counterclockwise)

Preceding gene: 54025802

Following gene: 54025800

Centisome position: 67.29

GC content: 73.04

Gene sequence:

>942_bases
ATGAGGGTGCCGATCGCGGTGGTCGGTCCCACCGCCACCGGCAAATCCGAGCTCGGGCTCGCGCTCGCCGAACACCTCGA
CGGCGAGATCGTCAACATCGACGCCATGCAGCTCTACCGTGGCATGGACATCGGCACCGCCAAGCTGCCGGTCGCCGAAC
GCCGCGGCATCCCGCACCACCAGCTCGACGTCCTCGACGTGACCGAGACCGCGAGCGTGGCCACCTATCAGCGCAACGCC
GCCGCCGACGTCGAGGCGATCCTGGCGCGCGGGCGCACACCGGTGATCGTGGGCGGCTCGATGATGTATGTCCAAGCGTT
GCTGGACGACTGGGAGTTCCCGGCCACCGATCCGGCGGTGCGCGCGAAGTGGGAGCGGCTGCTGGCGACCGAGGGCGTGG
CCGCGGTGCACGCGGCGCTGCGCGCGGCCGATCCGGCGGCGGCGGCGACCATTCTGCCCACCGACGGGCGGCGCATGGTG
CGCGCGCTCGAGGTCGTCGAGCTCACCGGCAAACCGTTCGCCGCGTCGGCCCCGCGCATCGGGACACCGCGCTGGGGCAC
GATCATCCTCGGCGTGGACCGCGACACCGCCGAACTCGACGAGCGGATCGCGCGCCGCACCGCCCTGATGTTCGACAGCG
GCCTGGTGGCGGAGGTGCGCGGCCTGGTCGAGCGCGGACTGCGCGAGGGCGTGACCGCGCGGCGGGCGATCGGCTACGCC
CACGTCCTGGCCTATCTCGACAACGAATACGACCTCGAACACGCCAAGGAGCGCACGCTGATCGGCACCCGGCGCTATGT
GCGGCGGCAGCGCTCGTGGTTCCGGCGCGACCCGCGGGTGCGCTGGCTCGACGGCGCCGACCCCGGCCTCGTGACCACGG
CACTGGCCCTGCTCGGCGACGCCGCGGCGGCGCCCGCGACCACGGAACGGACCACGCAGTGA

Upstream 100 bases:

>100_bases
CCGACCCCGCGGTGCGCACCTACTATCGGGACGCGCCGTTCGGCGTCGGCTATCACGTCAGCGTGTGGCGTCCGGGGGAG
TATCGGGAAGGCAGGGCCGG

Downstream 100 bases:

>100_bases
CCCGACGAGTCAGCACTCGCAACGCCTCGGTGCAGGTGTGGCAGGCCTATCTGACCAACCGCACCAAACGCCATCGCGAC
CGCCGCTTCCTCGTGCAGGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRVPIAVVGPTATGKSELGLALAEHLDGEIVNIDAMQLYRGMDIGTAKLPVAERRGIPHHQLDVLDVTETASVATYQRNA
AADVEAILARGRTPVIVGGSMMYVQALLDDWEFPATDPAVRAKWERLLATEGVAAVHAALRAADPAAAATILPTDGRRMV
RALEVVELTGKPFAASAPRIGTPRWGTIILGVDRDTAELDERIARRTALMFDSGLVAEVRGLVERGLREGVTARRAIGYA
HVLAYLDNEYDLEHAKERTLIGTRRYVRRQRSWFRRDPRVRWLDGADPGLVTTALALLGDAAAAPATTERTTQ

Sequences:

>Translated_313_residues
MRVPIAVVGPTATGKSELGLALAEHLDGEIVNIDAMQLYRGMDIGTAKLPVAERRGIPHHQLDVLDVTETASVATYQRNA
AADVEAILARGRTPVIVGGSMMYVQALLDDWEFPATDPAVRAKWERLLATEGVAAVHAALRAADPAAAATILPTDGRRMV
RALEVVELTGKPFAASAPRIGTPRWGTIILGVDRDTAELDERIARRTALMFDSGLVAEVRGLVERGLREGVTARRAIGYA
HVLAYLDNEYDLEHAKERTLIGTRRYVRRQRSWFRRDPRVRWLDGADPGLVTTALALLGDAAAAPATTERTTQ
>Mature_313_residues
MRVPIAVVGPTATGKSELGLALAEHLDGEIVNIDAMQLYRGMDIGTAKLPVAERRGIPHHQLDVLDVTETASVATYQRNA
AADVEAILARGRTPVIVGGSMMYVQALLDDWEFPATDPAVRAKWERLLATEGVAAVHAALRAADPAAAATILPTDGRRMV
RALEVVELTGKPFAASAPRIGTPRWGTIILGVDRDTAELDERIARRTALMFDSGLVAEVRGLVERGLREGVTARRAIGYA
HVLAYLDNEYDLEHAKERTLIGTRRYVRRQRSWFRRDPRVRWLDGADPGLVTTALALLGDAAAAPATTERTTQ

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=307, Percent_Identity=29.9674267100977, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790613, Length=290, Percent_Identity=37.2413793103448, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25144712, Length=178, Percent_Identity=32.0224719101124, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324848, Length=286, Percent_Identity=29.7202797202797, Blast_Score=142, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24649830, Length=355, Percent_Identity=29.0140845070423, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_NOCFA (Q5YT12)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120043.1
- ProteinModelPortal:   Q5YT12
- SMR:   Q5YT12
- GeneID:   3111292
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa38310
- NMPDR:   fig|247156.1.peg.3732
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   NFAR247156:NFA38310-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34008; Mature: 34008

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVPIAVVGPTATGKSELGLALAEHLDGEIVNIDAMQLYRGMDIGTAKLPVAERRGIPHH
CCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCHHHHCCCCCC
QLDVLDVTETASVATYQRNAAADVEAILARGRTPVIVGGSMMYVQALLDDWEFPATDPAV
CEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCCCCCHHH
RAKWERLLATEGVAAVHAALRAADPAAAATILPTDGRRMVRALEVVELTGKPFAASAPRI
HHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCC
GTPRWGTIILGVDRDTAELDERIARRTALMFDSGLVAEVRGLVERGLREGVTARRAIGYA
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
HVLAYLDNEYDLEHAKERTLIGTRRYVRRQRSWFRRDPRVRWLDGADPGLVTTALALLGD
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHC
AAAAPATTERTTQ
HHCCCCCCCCCCC
>Mature Secondary Structure
MRVPIAVVGPTATGKSELGLALAEHLDGEIVNIDAMQLYRGMDIGTAKLPVAERRGIPHH
CCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCHHHHCCCCCC
QLDVLDVTETASVATYQRNAAADVEAILARGRTPVIVGGSMMYVQALLDDWEFPATDPAV
CEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCCCCCHHH
RAKWERLLATEGVAAVHAALRAADPAAAATILPTDGRRMVRALEVVELTGKPFAASAPRI
HHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCC
GTPRWGTIILGVDRDTAELDERIARRTALMFDSGLVAEVRGLVERGLREGVTARRAIGYA
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
HVLAYLDNEYDLEHAKERTLIGTRRYVRRQRSWFRRDPRVRWLDGADPGLVTTALALLGD
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHC
AAAAPATTERTTQ
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA