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The map label for this gene is thrS [H]

Identifier: 54025682

GI number: 54025682

Start: 3925662

End: 3927773

Strand: Reverse

Name: thrS [H]

Synonym: nfa37120

Alternate gene names: 54025682

Gene position: 3927773-3925662 (Counterclockwise)

Preceding gene: 54025683

Following gene: 54025681

Centisome position: 65.23

GC content: 68.8

Gene sequence:

>2112_bases
ATGCCGCGCGGGAACCTGCCCGCCCACGAGAACGCCAAGGAGAGCGCCGCCGTGACGACCTCAGCCCCGATCAGTCCGTC
CACCCTCGTCCGGGTGCCGGCCGGGACTACGGCGGGCGCCGCGGTACGGGAGGCGGGCCTGCCGACCAAGGGCCCCGACA
CCATCGTCGTCGTCCGCGACGCCGAAGGCGCCCTGCGCGACCTGTCCTGGACCCCCGACACCGACGTCGAGGTGGAGGCC
GTGCCCGCCTCGACCGAAGCCGGGCGCAGCGTGATCCGCCATTCGGCCGCGCACGTGCTGGCCCAGGCCGTGCAGCAGGA
GTTCCCCGAGGCCAAACTCGGCATCGGCCCGCCCATCAAAGACGGCTTCTACTACGACTTCCAGGTCGAGCGCCCCTTCA
CCCCGGAGGATCTGGCCCGGCTGGAATCGCGGATGAAGAAGATCATCAAAGGCGCGCAGCGCTTCTCGCGCCGGGTGGTC
GAGATCGAGGAGGCCAGGGCCGAGCTGGCGAACGAGCCGTTCAAGCTCGAGCTGATCAGCGACAAGTCCGGCATCGACGA
CCCCGAGGTCATGGAGGTGGGCGGCAAGGAGCTGACCATCTACGACAATCTCGACCCGCGCACGGGCGAGAAGGTGTGGG
GCGACCTGTGCCGCGGCCCGCACATCCCCACCACCAAGTTCATCCCCGCCTTCAAGCTCACCCGCAGCTCGGCCGCGTAC
TGGCGCGGTGACCAGAACCGCGAGGACCTGCAGCGCATCTACGGCACCGCCTGGGAATCGCCGGAGGCGCTGGAGGCACA
CCTGCACGTGCTCGCCGAGGCCGAGCGCCGTGACCACCGCAAGCTGGGCCTGGAGCTGGACCTGTTCAGCTTCCCCGACG
AACTCGGCTCGGGCCTGCCGGTGTTCCATCCCAAGGGCGGCATCATCCGCAAGGAGCTCGAGGAGTACTCGCGGCGCAGG
CACGTGGACGCGGGCTACGAGTTCGTCAACACCCCGCACATCACCAAGGGCCACCTGTTCGAGGTGTCCGGCCACCTGGA
CTGGTACCGCGACGGCATGTTCCCGGCCATGCACCTGGACGCGGAGTACAACGACGACGGCACCGTGCGCAAGCCCGGCC
AGGACTACTACGTCAAGCCGATGAACTGCCCGATGCACAACCTGATCTTCCGGGCGCGCGGGCGGTCCTACCGCGAGCTG
CCGCTGCGGCTGTTCGAATTCGGCTCGGTGTACCGCTACGAGAAGTCCGGCGTGGTGCACGGCCTGACCCGGGTGCGCGG
CATGACCCAGGACGACGCGCACATCTACTGCACCAAGGAGCAGATGCACGCCGAGCTCACGAACACCCTGCAGTTCGTGC
TCGGCCTGCTCGCCGACTACGGCCTCGACGACTTCTACCTCGAGCTGTCCACCAAGGACCCGAAGAAGTTCGTCGGCTCC
GACGAGATCTGGGAGGAGGCCACCGAGACGCTGTCGAAGGTGGCCCAGGCCTCGGGTCTCGAGCTCGTGCCCGATCCGGG
CGGCGCCGCGTTCTACGGCCCGAAGATCTCCGTGCAGGCCAAGGACGCGCTCGGCCGCACCTGGCAGATGTCGACCATCC
AGCTCGACTTCAACCTGCCCGAGCGCTTCGGCCTGGAGTACACCGCCCCCGACGGCAGCAAGCAGCGGCCGGTGATGATC
CACCGCGCGCTGTTCGGGTCCATCGAGCGGTTCTTCGGCGTGCTCACCGAGCACTACGCGGGCGCCTTCCCGGCCTGGCT
GTCGCCGGTGCAGGTGGTGGGCATCCCGGTCGCGGAGGCCTTCGCCCCGCACCTGGACCGGGTCGTCGAGCTGCTGCGCG
ACCACGGCGTGCGCGCGCAGGTCGACCGCAGCGACGATCGGATGCAGAAGAAGATCTTCAACAACACCGCGCAGAAGGTG
CCGTTCATGCTGCTGGCCGGCGAGCGCGATGTGAACGCGGGCGCGGTGAGTTTCCGGTTCCGGGACGGCACCCAGGTCAA
CGGGGTGCCGGTCGCCGACGCGGTGGCGACCATCGTGGCGTGGATCACCAACCGGGAGAACGCTTCTCCGACCGCGGAGG
GGTTCGAGATCGTCACGGCGGGGGAGGGATAG

Upstream 100 bases:

>100_bases
AGCAGCGGTGGCGGGGCGAGCGCCGATCACCCGGTCCATCGGCGCGCCGGGGTGCCGGTGCCGATAGCATGGTCGCGCCG
GAGCGGATACGCCGCCGGTG

Downstream 100 bases:

>100_bases
ATGAGCGCTGCGGAGGAGACGACGGACCGCGGCGGTGACATCGTGGACATCGGTGCGGGCGAACCCGATCGGCTGCAACG
GCTCTGGACGCCGTACCGGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MPRGNLPAHENAKESAAVTTSAPISPSTLVRVPAGTTAGAAVREAGLPTKGPDTIVVVRDAEGALRDLSWTPDTDVEVEA
VPASTEAGRSVIRHSAAHVLAQAVQQEFPEAKLGIGPPIKDGFYYDFQVERPFTPEDLARLESRMKKIIKGAQRFSRRVV
EIEEARAELANEPFKLELISDKSGIDDPEVMEVGGKELTIYDNLDPRTGEKVWGDLCRGPHIPTTKFIPAFKLTRSSAAY
WRGDQNREDLQRIYGTAWESPEALEAHLHVLAEAERRDHRKLGLELDLFSFPDELGSGLPVFHPKGGIIRKELEEYSRRR
HVDAGYEFVNTPHITKGHLFEVSGHLDWYRDGMFPAMHLDAEYNDDGTVRKPGQDYYVKPMNCPMHNLIFRARGRSYREL
PLRLFEFGSVYRYEKSGVVHGLTRVRGMTQDDAHIYCTKEQMHAELTNTLQFVLGLLADYGLDDFYLELSTKDPKKFVGS
DEIWEEATETLSKVAQASGLELVPDPGGAAFYGPKISVQAKDALGRTWQMSTIQLDFNLPERFGLEYTAPDGSKQRPVMI
HRALFGSIERFFGVLTEHYAGAFPAWLSPVQVVGIPVAEAFAPHLDRVVELLRDHGVRAQVDRSDDRMQKKIFNNTAQKV
PFMLLAGERDVNAGAVSFRFRDGTQVNGVPVADAVATIVAWITNRENASPTAEGFEIVTAGEG

Sequences:

>Translated_703_residues
MPRGNLPAHENAKESAAVTTSAPISPSTLVRVPAGTTAGAAVREAGLPTKGPDTIVVVRDAEGALRDLSWTPDTDVEVEA
VPASTEAGRSVIRHSAAHVLAQAVQQEFPEAKLGIGPPIKDGFYYDFQVERPFTPEDLARLESRMKKIIKGAQRFSRRVV
EIEEARAELANEPFKLELISDKSGIDDPEVMEVGGKELTIYDNLDPRTGEKVWGDLCRGPHIPTTKFIPAFKLTRSSAAY
WRGDQNREDLQRIYGTAWESPEALEAHLHVLAEAERRDHRKLGLELDLFSFPDELGSGLPVFHPKGGIIRKELEEYSRRR
HVDAGYEFVNTPHITKGHLFEVSGHLDWYRDGMFPAMHLDAEYNDDGTVRKPGQDYYVKPMNCPMHNLIFRARGRSYREL
PLRLFEFGSVYRYEKSGVVHGLTRVRGMTQDDAHIYCTKEQMHAELTNTLQFVLGLLADYGLDDFYLELSTKDPKKFVGS
DEIWEEATETLSKVAQASGLELVPDPGGAAFYGPKISVQAKDALGRTWQMSTIQLDFNLPERFGLEYTAPDGSKQRPVMI
HRALFGSIERFFGVLTEHYAGAFPAWLSPVQVVGIPVAEAFAPHLDRVVELLRDHGVRAQVDRSDDRMQKKIFNNTAQKV
PFMLLAGERDVNAGAVSFRFRDGTQVNGVPVADAVATIVAWITNRENASPTAEGFEIVTAGEG
>Mature_702_residues
PRGNLPAHENAKESAAVTTSAPISPSTLVRVPAGTTAGAAVREAGLPTKGPDTIVVVRDAEGALRDLSWTPDTDVEVEAV
PASTEAGRSVIRHSAAHVLAQAVQQEFPEAKLGIGPPIKDGFYYDFQVERPFTPEDLARLESRMKKIIKGAQRFSRRVVE
IEEARAELANEPFKLELISDKSGIDDPEVMEVGGKELTIYDNLDPRTGEKVWGDLCRGPHIPTTKFIPAFKLTRSSAAYW
RGDQNREDLQRIYGTAWESPEALEAHLHVLAEAERRDHRKLGLELDLFSFPDELGSGLPVFHPKGGIIRKELEEYSRRRH
VDAGYEFVNTPHITKGHLFEVSGHLDWYRDGMFPAMHLDAEYNDDGTVRKPGQDYYVKPMNCPMHNLIFRARGRSYRELP
LRLFEFGSVYRYEKSGVVHGLTRVRGMTQDDAHIYCTKEQMHAELTNTLQFVLGLLADYGLDDFYLELSTKDPKKFVGSD
EIWEEATETLSKVAQASGLELVPDPGGAAFYGPKISVQAKDALGRTWQMSTIQLDFNLPERFGLEYTAPDGSKQRPVMIH
RALFGSIERFFGVLTEHYAGAFPAWLSPVQVVGIPVAEAFAPHLDRVVELLRDHGVRAQVDRSDDRMQKKIFNNTAQKVP
FMLLAGERDVNAGAVSFRFRDGTQVNGVPVADAVATIVAWITNRENASPTAEGFEIVTAGEG

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=627, Percent_Identity=37.6395534290271, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI38570111, Length=623, Percent_Identity=37.0786516853933, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI20070344, Length=648, Percent_Identity=36.1111111111111, Blast_Score=340, Evalue=2e-93,
Organism=Escherichia coli, GI1788013, Length=625, Percent_Identity=38.24, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71984192, Length=617, Percent_Identity=35.8184764991896, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71984184, Length=617, Percent_Identity=35.8184764991896, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6322112, Length=647, Percent_Identity=37.0942812982998, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6322654, Length=406, Percent_Identity=35.7142857142857, Blast_Score=242, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24583842, Length=632, Percent_Identity=35.6012658227848, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI28574141, Length=632, Percent_Identity=35.6012658227848, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24583839, Length=632, Percent_Identity=35.6012658227848, Blast_Score=396, Evalue=1e-110,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 78235; Mature: 78104

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGNLPAHENAKESAAVTTSAPISPSTLVRVPAGTTAGAAVREAGLPTKGPDTIVVVRD
CCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCEEEEEEC
AEGALRDLSWTPDTDVEVEAVPASTEAGRSVIRHSAAHVLAQAVQQEFPEAKLGIGPPIK
CCCHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DGFYYDFQVERPFTPEDLARLESRMKKIIKGAQRFSRRVVEIEEARAELANEPFKLELIS
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
DKSGIDDPEVMEVGGKELTIYDNLDPRTGEKVWGDLCRGPHIPTTKFIPAFKLTRSSAAY
CCCCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCHHCCCCCCHH
WRGDQNREDLQRIYGTAWESPEALEAHLHVLAEAERRDHRKLGLELDLFSFPDELGSGLP
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCC
VFHPKGGIIRKELEEYSRRRHVDAGYEFVNTPHITKGHLFEVSGHLDWYRDGMFPAMHLD
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHCCCCCEEEEC
AEYNDDGTVRKPGQDYYVKPMNCPMHNLIFRARGRSYRELPLRLFEFGSVYRYEKSGVVH
CCCCCCCCEECCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCHHH
GLTRVRGMTQDDAHIYCTKEQMHAELTNTLQFVLGLLADYGLDDFYLELSTKDPKKFVGS
HHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCC
DEIWEEATETLSKVAQASGLELVPDPGGAAFYGPKISVQAKDALGRTWQMSTIQLDFNLP
HHHHHHHHHHHHHHHHHCCCEEECCCCCCEEECCEEEEEEHHHCCCEEEEEEEEEEECCH
ERFGLEYTAPDGSKQRPVMIHRALFGSIERFFGVLTEHYAGAFPAWLSPVQVVGIPVAEA
HHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCHHHH
FAPHLDRVVELLRDHGVRAQVDRSDDRMQKKIFNNTAQKVPFMLLAGERDVNAGAVSFRF
HHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEE
RDGTQVNGVPVADAVATIVAWITNRENASPTAEGFEIVTAGEG
CCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
PRGNLPAHENAKESAAVTTSAPISPSTLVRVPAGTTAGAAVREAGLPTKGPDTIVVVRD
CCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCEEEEEEC
AEGALRDLSWTPDTDVEVEAVPASTEAGRSVIRHSAAHVLAQAVQQEFPEAKLGIGPPIK
CCCHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DGFYYDFQVERPFTPEDLARLESRMKKIIKGAQRFSRRVVEIEEARAELANEPFKLELIS
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
DKSGIDDPEVMEVGGKELTIYDNLDPRTGEKVWGDLCRGPHIPTTKFIPAFKLTRSSAAY
CCCCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCHHCCCCCCHH
WRGDQNREDLQRIYGTAWESPEALEAHLHVLAEAERRDHRKLGLELDLFSFPDELGSGLP
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCC
VFHPKGGIIRKELEEYSRRRHVDAGYEFVNTPHITKGHLFEVSGHLDWYRDGMFPAMHLD
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHCCCCCEEEEC
AEYNDDGTVRKPGQDYYVKPMNCPMHNLIFRARGRSYRELPLRLFEFGSVYRYEKSGVVH
CCCCCCCCEECCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCHHH
GLTRVRGMTQDDAHIYCTKEQMHAELTNTLQFVLGLLADYGLDDFYLELSTKDPKKFVGS
HHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCC
DEIWEEATETLSKVAQASGLELVPDPGGAAFYGPKISVQAKDALGRTWQMSTIQLDFNLP
HHHHHHHHHHHHHHHHHCCCEEECCCCCCEEECCEEEEEEHHHCCCEEEEEEEEEEECCH
ERFGLEYTAPDGSKQRPVMIHRALFGSIERFFGVLTEHYAGAFPAWLSPVQVVGIPVAEA
HHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCHHHH
FAPHLDRVVELLRDHGVRAQVDRSDDRMQKKIFNNTAQKVPFMLLAGERDVNAGAVSFRF
HHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEE
RDGTQVNGVPVADAVATIVAWITNRENASPTAEGFEIVTAGEG
CCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA