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The map label for this gene is pdxT [H]

Identifier: 54025673

GI number: 54025673

Start: 3918354

End: 3919100

Strand: Reverse

Name: pdxT [H]

Synonym: nfa37030

Alternate gene names: 54025673

Gene position: 3919100-3918354 (Counterclockwise)

Preceding gene: 54025674

Following gene: 54025672

Centisome position: 65.09

GC content: 72.42

Gene sequence:

>747_bases
GTGACCGGGCGTGAGCCGGGCCACAGCGCGGCCGCTCGTAAGCTGGGCCGGGTGAACCAGACCTCGGTGTCCGCACCCGC
CGCCGAGCCGCAGGCGGCCCCGTCCCGGCCCACCATCGGCGTGCTCGCCCTGCAGGGCGATGTCCGCGAACACCTCGCCG
CGCTGCGCGCGTGCGGTGCCGAACCCGTGCTGGTGCGCCGGGTCGGCGAACTCGAGGCCGTGGACGGGCTGGTGCTGCCG
GGCGGGGAGTCCACCGCGATCAGCAAACTGCTGCAGGTCTTCGACCTGCTCGATCCGCTGCGCGCGCGGCTGCGGGACGG
TATGCCCGCCTTCGGCTCGTGTGCCGGGATGATCCTGTTGGCCTCGGAGGTGCTCGACACCCGGCCCGACGCCCAGCACC
TGTCGGGCATCGACATGACGGTGCGCCGCAACGCGTTCGGACGTCAGGTCGACTCCTTCGAGACCGACCTGGATTTCGCG
GGCCTCGACGACGGCCCGGTGCGCGCGGTCTTCATCCGAGCGCCGTGGGTCGAGCGCGCGGGCGACGGTGTGCAGGTGCT
GGCCACCGTGCCCGACGGCCCGTCGCGGGGCCGGATCGTGGCCGTGCGCCAGGGCAACGTGCTGGCCACGTCGTTCCATC
CGGAGGTCACCGGTGACCTGCGGGTACACCGGATGTTCGTCGACATCGTGCGGGCCGCCGGAGACCGAGCGGGTAACGTA
CACACTCCAGACGTGCAACCGACCTGA

Upstream 100 bases:

>100_bases
CGGGCTTCGGTCGCGGCCTGACCTCCGGCAAGATCTACAACCGGGCCGGTGAACTGGTCGCCGCGGTGATGCAGGAGGGC
CTGATCCGCACGTTGCGCTC

Downstream 100 bases:

>100_bases
AGGAAGTAGGGGAATGAGCGGCCACTCCAAATGGGCCACCACCAAGCACAAGAAGGCCGCGATCGACGCGAAGCGGGGGA
AGCTCTTCGCGAAGTTGATC

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTGREPGHSAAARKLGRVNQTSVSAPAAEPQAAPSRPTIGVLALQGDVREHLAALRACGAEPVLVRRVGELEAVDGLVLP
GGESTAISKLLQVFDLLDPLRARLRDGMPAFGSCAGMILLASEVLDTRPDAQHLSGIDMTVRRNAFGRQVDSFETDLDFA
GLDDGPVRAVFIRAPWVERAGDGVQVLATVPDGPSRGRIVAVRQGNVLATSFHPEVTGDLRVHRMFVDIVRAAGDRAGNV
HTPDVQPT

Sequences:

>Translated_248_residues
MTGREPGHSAAARKLGRVNQTSVSAPAAEPQAAPSRPTIGVLALQGDVREHLAALRACGAEPVLVRRVGELEAVDGLVLP
GGESTAISKLLQVFDLLDPLRARLRDGMPAFGSCAGMILLASEVLDTRPDAQHLSGIDMTVRRNAFGRQVDSFETDLDFA
GLDDGPVRAVFIRAPWVERAGDGVQVLATVPDGPSRGRIVAVRQGNVLATSFHPEVTGDLRVHRMFVDIVRAAGDRAGNV
HTPDVQPT
>Mature_247_residues
TGREPGHSAAARKLGRVNQTSVSAPAAEPQAAPSRPTIGVLALQGDVREHLAALRACGAEPVLVRRVGELEAVDGLVLPG
GESTAISKLLQVFDLLDPLRARLRDGMPAFGSCAGMILLASEVLDTRPDAQHLSGIDMTVRRNAFGRQVDSFETDLDFAG
LDDGPVRAVFIRAPWVERAGDGVQVLATVPDGPSRGRIVAVRQGNVLATSFHPEVTGDLRVHRMFVDIVRAAGDRAGNVH
TPDVQPT

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS [H]

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=213, Percent_Identity=36.150234741784, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323995, Length=211, Percent_Identity=37.4407582938389, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321048, Length=211, Percent_Identity=36.9668246445498, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002161
- InterPro:   IPR021196 [H]

Pfam domain/function: PF01174 SNO [H]

EC number: NA

Molecular weight: Translated: 26267; Mature: 26136

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51130 PDXT_SNO_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGREPGHSAAARKLGRVNQTSVSAPAAEPQAAPSRPTIGVLALQGDVREHLAALRACGA
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCC
EPVLVRRVGELEAVDGLVLPGGESTAISKLLQVFDLLDPLRARLRDGMPAFGSCAGMILL
CHHHHHHHCCHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ASEVLDTRPDAQHLSGIDMTVRRNAFGRQVDSFETDLDFAGLDDGPVRAVFIRAPWVERA
HHHHHHCCCCHHHHCCCCCEEHHCHHHCHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHC
GDGVQVLATVPDGPSRGRIVAVRQGNVLATSFHPEVTGDLRVHRMFVDIVRAAGDRAGNV
CCCEEEEEECCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
HTPDVQPT
CCCCCCCC
>Mature Secondary Structure 
TGREPGHSAAARKLGRVNQTSVSAPAAEPQAAPSRPTIGVLALQGDVREHLAALRACGA
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCC
EPVLVRRVGELEAVDGLVLPGGESTAISKLLQVFDLLDPLRARLRDGMPAFGSCAGMILL
CHHHHHHHCCHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ASEVLDTRPDAQHLSGIDMTVRRNAFGRQVDSFETDLDFAGLDDGPVRAVFIRAPWVERA
HHHHHHCCCCHHHHCCCCCEEHHCHHHCHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHC
GDGVQVLATVPDGPSRGRIVAVRQGNVLATSFHPEVTGDLRVHRMFVDIVRAAGDRAGNV
CCCEEEEEECCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
HTPDVQPT
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA