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The map label for this gene is mdh

Identifier: 54025632

GI number: 54025632

Start: 3877656

End: 3878660

Strand: Reverse

Name: mdh

Synonym: nfa36620

Alternate gene names: 54025632

Gene position: 3878660-3877656 (Counterclockwise)

Preceding gene: 54025633

Following gene: 54025627

Centisome position: 64.42

GC content: 72.44

Gene sequence:

>1005_bases
GTGAGCACCGCCGACCGCACCGCCCCCGTGACCGTCACCGTGACCGGTGCCGCCGGGCAGATCGCCTACGGCCTGCTGTT
CCGCATCGCCTCCGGGGCCATGCTCGGCCCGCACACGCCGATCCGGCTGCGGCTGTTGGAGATTCCCGCCGCCGTCGCCT
CGCTGGAGGGGGTGGCGATGGAGCTGGAGGACGGCGCGTTCCCGCTGCTGGACGCCATCGACATCAGCGACGACCCGTGG
ACCGGCTTCGCCGGGGCGAACGTGGCGCTGCTGGTCGGCGCCCGGCCGCGCACCGCGGGCATGGAGCGTGCCGACCTGCT
GGCCGCCAACGGCCCGATCTTCACCGACCAGGGCCAGGCGATCAACGCCGTCGCCGCCGACGACGTGAAGGTGCTCGTCG
TCGGCAATCCCGCCAACACCAACGCGTTCATCGCGATGAGCAACGCACCCGACGTACCCGCCGAGCGGTTCACCGCGATG
ACCCGCCTCGACCACAACCGCGCCATCGCGCAGCTGGCGAAGAAGACCGGCGCACCGGCGACCGAGATCCATCGGATCGC
GGTGTGGGGCAACCACTCCGCCACCCAGTACCCCGACCTCACGCACGCCACGGTCGCGGGCCGCCCGGCGCGCGAGCTGG
TCGACGAGCAGTGGCTGCGCGAGGACTTCATCCCCACCGTGCAGCAGCGCGGCACCGCGATCATCCAGGCGCGCGGTGCC
TCCTCGGCCGCCAGCGCGGCCAGCGCCGCCATCGACCACATCCACGACTGGGTGCTCGGCACCCCGGCGGGGGAGTGGAC
CTCGATGGCGGTGCCCTCGGACGGGTCCTACGGTGTGCCGGACGGGCTGATCAGCTCGTTCCCGGTGACCTGCGCCGACG
GTGCCTACCGGATCGTCGAGGGACTCGAGGTCGACGCGTTCTCGCGCGAGCGCATCGATGCCTCGGTCGCCGAGCTGACC
GCCGAACGCGAGGCGGTGGTGGAGCTCGGCTTCGCCAAGGGCTGA

Upstream 100 bases:

>100_bases
CCGAACGACCCTGGTGTTCCGTAGCTCACCACCGCGGTTCGGCCTCGGTGAACCTCGCTAGGGTGGGCAGCGAGACGCAG
TCGACCCCGAGGGAGTTCTC

Downstream 100 bases:

>100_bases
GCCCGGCTACCGCACCGTCCAGGTCTCCGGGCCGTTGAGCAGGCGTTGTAGCGAATCCGCTTGCACCGGAGCCTGTTCGG
CGGCGGCGGCGTTCTGGGCA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSTADRTAPVTVTVTGAAGQIAYGLLFRIASGAMLGPHTPIRLRLLEIPAAVASLEGVAMELEDGAFPLLDAIDISDDPW
TGFAGANVALLVGARPRTAGMERADLLAANGPIFTDQGQAINAVAADDVKVLVVGNPANTNAFIAMSNAPDVPAERFTAM
TRLDHNRAIAQLAKKTGAPATEIHRIAVWGNHSATQYPDLTHATVAGRPARELVDEQWLREDFIPTVQQRGTAIIQARGA
SSAASAASAAIDHIHDWVLGTPAGEWTSMAVPSDGSYGVPDGLISSFPVTCADGAYRIVEGLEVDAFSRERIDASVAELT
AEREAVVELGFAKG

Sequences:

>Translated_334_residues
MSTADRTAPVTVTVTGAAGQIAYGLLFRIASGAMLGPHTPIRLRLLEIPAAVASLEGVAMELEDGAFPLLDAIDISDDPW
TGFAGANVALLVGARPRTAGMERADLLAANGPIFTDQGQAINAVAADDVKVLVVGNPANTNAFIAMSNAPDVPAERFTAM
TRLDHNRAIAQLAKKTGAPATEIHRIAVWGNHSATQYPDLTHATVAGRPARELVDEQWLREDFIPTVQQRGTAIIQARGA
SSAASAASAAIDHIHDWVLGTPAGEWTSMAVPSDGSYGVPDGLISSFPVTCADGAYRIVEGLEVDAFSRERIDASVAELT
AEREAVVELGFAKG
>Mature_333_residues
STADRTAPVTVTVTGAAGQIAYGLLFRIASGAMLGPHTPIRLRLLEIPAAVASLEGVAMELEDGAFPLLDAIDISDDPWT
GFAGANVALLVGARPRTAGMERADLLAANGPIFTDQGQAINAVAADDVKVLVVGNPANTNAFIAMSNAPDVPAERFTAMT
RLDHNRAIAQLAKKTGAPATEIHRIAVWGNHSATQYPDLTHATVAGRPARELVDEQWLREDFIPTVQQRGTAIIQARGAS
SAASAASAAIDHIHDWVLGTPAGEWTSMAVPSDGSYGVPDGLISSFPVTCADGAYRIVEGLEVDAFSRERIDASVAELTA
EREAVVELGFAKG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=329, Percent_Identity=50.4559270516717, Blast_Score=332, Evalue=2e-91,
Organism=Homo sapiens, GI89886456, Length=336, Percent_Identity=26.1904761904762, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17561064, Length=324, Percent_Identity=52.7777777777778, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI32566798, Length=263, Percent_Identity=52.0912547528517, Blast_Score=246, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=54.7058823529412, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24583394, Length=321, Percent_Identity=51.0903426791277, Blast_Score=305, Evalue=3e-83,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_NOCFA (Q5YTI1)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119874.1
- ProteinModelPortal:   Q5YTI1
- SMR:   Q5YTI1
- GeneID:   3111271
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa36620
- NMPDR:   fig|247156.1.peg.3572
- HOGENOM:   HBG289884
- OMA:   PQGRAIN
- ProtClustDB:   PRK05442
- BioCyc:   NFAR247156:NFA36620-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34911; Mature: 34779

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 192-192 BINDING 97-97 BINDING 103-103 BINDING 110-110 BINDING 117-117 BINDING 136-136 BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTADRTAPVTVTVTGAAGQIAYGLLFRIASGAMLGPHTPIRLRLLEIPAAVASLEGVAM
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHCCEEE
ELEDGAFPLLDAIDISDDPWTGFAGANVALLVGARPRTAGMERADLLAANGPIFTDQGQA
EECCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHEEECCCCEECCCCCE
INAVAADDVKVLVVGNPANTNAFIAMSNAPDVPAERFTAMTRLDHNRAIAQLAKKTGAPA
EEEEECCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
TEIHRIAVWGNHSATQYPDLTHATVAGRPARELVDEQWLREDFIPTVQQRGTAIIQARGA
HHEEEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCHHHHHCCCEEEEECCC
SSAASAASAAIDHIHDWVLGTPAGEWTSMAVPSDGSYGVPDGLISSFPVTCADGAYRIVE
CHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCCCCCCCHHHHCCCCEECCCHHHHHH
GLEVDAFSRERIDASVAELTAEREAVVELGFAKG
CCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCC
>Mature Secondary Structure 
STADRTAPVTVTVTGAAGQIAYGLLFRIASGAMLGPHTPIRLRLLEIPAAVASLEGVAM
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHCCEEE
ELEDGAFPLLDAIDISDDPWTGFAGANVALLVGARPRTAGMERADLLAANGPIFTDQGQA
EECCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHEEECCCCEECCCCCE
INAVAADDVKVLVVGNPANTNAFIAMSNAPDVPAERFTAMTRLDHNRAIAQLAKKTGAPA
EEEEECCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
TEIHRIAVWGNHSATQYPDLTHATVAGRPARELVDEQWLREDFIPTVQQRGTAIIQARGA
HHEEEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCHHHHHCCCEEEEECCC
SSAASAASAAIDHIHDWVLGTPAGEWTSMAVPSDGSYGVPDGLISSFPVTCADGAYRIVE
CHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCCCCCCCHHHHCCCCEECCCHHHHHH
GLEVDAFSRERIDASVAELTAEREAVVELGFAKG
CCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA