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The map label for this gene is metK

Identifier: 54025583

GI number: 54025583

Start: 3824678

End: 3825892

Strand: Reverse

Name: metK

Synonym: nfa36130

Alternate gene names: 54025583

Gene position: 3825892-3824678 (Counterclockwise)

Preceding gene: 54025584

Following gene: 54025582

Centisome position: 63.54

GC content: 69.47

Gene sequence:

>1215_bases
ATGCGCACGTCTGGCAGCCGCCTATTCACCAGTGAGTCCGTGACCGAGGGTCACCCGGACAAGATCTGTGACGCGATCAG
CGATTCCATCCTCGACGCGTTGCTCGCCGAGGACCCGCGCAGCCGGGTCGCGGTGGAGACCCTCGTGACCACCGGTCAGG
TGCACGTCGCGGGCGAGGTCACCACCTCCGCCTACGCCGACATTCCGCGGATCGTGCGCGAGAAGGTCCTCGAGATCGGA
TACGACTCCTCGGCAAAGGGTTTCGACGGCAACTCCTGCGGCGTGAACATCGCCATCGGCGCGCAGTCGCCCGATATCGC
GCAGGGTGTGGACACCTCGCACGAGGCCCGGGTGGGCGGCACCGACGACGAGATCGCCAAGCAGGGCGCAGGTGACCAGG
GCCTGATGTTCGGTTACGCCACCACCGACACCCCCGAGCTGATGCCGCTGCCGATCGCGCTGGCGCACCGCCTCTCGCGC
AAGCTCACCGAGGTCCGCAAGACCGGCGTGCTGCCCTACCTGCGCCCGGACGGCAAGACCCAGGTCACCATCGAATACGA
CGGCGATCGCCCGGTCCGGCTGGACACCGTGGTCATCTCCACCCAGCACGCCGCCGACATCGACCTGGACAACCTGCTCG
CTCCCGACATCAGGGAGAAGGTCGTCGACGCGGTGCTCGCCGACCTGGACCTGCCCAGCCCCTTGGACACCTCCGACATC
CGGCTGCTGGTCAACCCCACCGGCAAGTTCGTGCTCGGCGGCCCGATGGGCGACGCCGGCCTGACCGGCCGCAAGATCAT
CGTCGACACCTACGGCGGCATGGCCCGCCACGGCGGCGGCGCCTTCTCGGGCAAGGACCCGTCGAAGGTCGACCGGTCGG
CCGCCTACGCCATGCGCTGGGTCGCCAAGAACGTCGTGGCCGCCGGCCTGTCCGAGCGGGTCGAGGTGCAGGTCGCCTAC
GCGATCGGCAAGGCCGCCCCGGTCGGTCTGTTCGTCGAGACCTTCGGCACCGAGAAGGTGGATCCGGCGCGTATCGCGGC
CGCGATCACCGAGGTGTTCGACCTGCGTCCCGGCGCGATCATCCGCGACCTGGACCTGCTGCGCCCGATCTACGCGCCGA
CCGCGGCCTACGGGCACTTCGGCCGCACCGACATCGATCTGCCCTGGGAGCACACCGATCGCGCGGACAAGCTGCGCGCG
GCCGCCGGACTCTAG

Upstream 100 bases:

>100_bases
GGAATAGTCTGCAGAGGAAGGGTTGCGCGGCGGCGACTTGGCACTACTGTCGCTGTCGCGATCCGATACGACGAACCCGT
TGAGGGAGAGGGAACAACTG

Downstream 100 bases:

>100_bases
GTCGACCGGGTCCGCGTGAGCCGACCCACCACGGACGGCGCATCGGCAGCCCCCGCGGACACCGCGTCCGCGGGGGCGTC
GGCGTCTTCGGCGCGCCGTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MRTSGSRLFTSESVTEGHPDKICDAISDSILDALLAEDPRSRVAVETLVTTGQVHVAGEVTTSAYADIPRIVREKVLEIG
YDSSAKGFDGNSCGVNIAIGAQSPDIAQGVDTSHEARVGGTDDEIAKQGAGDQGLMFGYATTDTPELMPLPIALAHRLSR
KLTEVRKTGVLPYLRPDGKTQVTIEYDGDRPVRLDTVVISTQHAADIDLDNLLAPDIREKVVDAVLADLDLPSPLDTSDI
RLLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLSERVEVQVAY
AIGKAAPVGLFVETFGTEKVDPARIAAAITEVFDLRPGAIIRDLDLLRPIYAPTAAYGHFGRTDIDLPWEHTDRADKLRA
AAGL

Sequences:

>Translated_404_residues
MRTSGSRLFTSESVTEGHPDKICDAISDSILDALLAEDPRSRVAVETLVTTGQVHVAGEVTTSAYADIPRIVREKVLEIG
YDSSAKGFDGNSCGVNIAIGAQSPDIAQGVDTSHEARVGGTDDEIAKQGAGDQGLMFGYATTDTPELMPLPIALAHRLSR
KLTEVRKTGVLPYLRPDGKTQVTIEYDGDRPVRLDTVVISTQHAADIDLDNLLAPDIREKVVDAVLADLDLPSPLDTSDI
RLLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLSERVEVQVAY
AIGKAAPVGLFVETFGTEKVDPARIAAAITEVFDLRPGAIIRDLDLLRPIYAPTAAYGHFGRTDIDLPWEHTDRADKLRA
AAGL
>Mature_404_residues
MRTSGSRLFTSESVTEGHPDKICDAISDSILDALLAEDPRSRVAVETLVTTGQVHVAGEVTTSAYADIPRIVREKVLEIG
YDSSAKGFDGNSCGVNIAIGAQSPDIAQGVDTSHEARVGGTDDEIAKQGAGDQGLMFGYATTDTPELMPLPIALAHRLSR
KLTEVRKTGVLPYLRPDGKTQVTIEYDGDRPVRLDTVVISTQHAADIDLDNLLAPDIREKVVDAVLADLDLPSPLDTSDI
RLLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLSERVEVQVAY
AIGKAAPVGLFVETFGTEKVDPARIAAAITEVFDLRPGAIIRDLDLLRPIYAPTAAYGHFGRTDIDLPWEHTDRADKLRA
AAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=391, Percent_Identity=54.9872122762148, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI4557737, Length=392, Percent_Identity=53.5714285714286, Blast_Score=392, Evalue=1e-109,
Organism=Escherichia coli, GI1789311, Length=398, Percent_Identity=60.5527638190955, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25145633, Length=396, Percent_Identity=54.5454545454545, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17538494, Length=388, Percent_Identity=54.1237113402062, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17538498, Length=388, Percent_Identity=54.639175257732, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551082, Length=392, Percent_Identity=50.765306122449, Blast_Score=373, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32565905, Length=388, Percent_Identity=49.7422680412371, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI25145635, Length=350, Percent_Identity=52.8571428571429, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI32565911, Length=342, Percent_Identity=52.6315789473684, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI32565903, Length=316, Percent_Identity=52.8481012658228, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=54.2168674698795, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323209, Length=391, Percent_Identity=54.9872122762148, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6320710, Length=397, Percent_Identity=54.6599496221663, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45552159, Length=394, Percent_Identity=50.761421319797, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580529, Length=394, Percent_Identity=50.761421319797, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580537, Length=394, Percent_Identity=50.761421319797, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580533, Length=394, Percent_Identity=50.761421319797, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580535, Length=394, Percent_Identity=50.761421319797, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580531, Length=394, Percent_Identity=50.761421319797, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580527, Length=394, Percent_Identity=50, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580525, Length=394, Percent_Identity=50, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580541, Length=389, Percent_Identity=48.0719794344473, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24580539, Length=126, Percent_Identity=49.2063492063492, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_NOCFA (Q5YTN0)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119825.1
- ProteinModelPortal:   Q5YTN0
- SMR:   Q5YTN0
- GeneID:   3111284
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa36130
- NMPDR:   fig|247156.1.peg.3526
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   NFAR247156:NFA36130-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42965; Mature: 42965

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSGSRLFTSESVTEGHPDKICDAISDSILDALLAEDPRSRVAVETLVTTGQVHVAGEV
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECC
TTSAYADIPRIVREKVLEIGYDSSAKGFDGNSCGVNIAIGAQSPDIAQGVDTSHEARVGG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCC
TDDEIAKQGAGDQGLMFGYATTDTPELMPLPIALAHRLSRKLTEVRKTGVLPYLRPDGKT
CHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCE
QVTIEYDGDRPVRLDTVVISTQHAADIDLDNLLAPDIREKVVDAVLADLDLPSPLDTSDI
EEEEEECCCCCEEEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCE
RLLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRW
EEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHH
VAKNVVAAGLSERVEVQVAYAIGKAAPVGLFVETFGTEKVDPARIAAAITEVFDLRPGAI
HHHHHHHHCCCHHEEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCH
IRDLDLLRPIYAPTAAYGHFGRTDIDLPWEHTDRADKLRAAAGL
HHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRTSGSRLFTSESVTEGHPDKICDAISDSILDALLAEDPRSRVAVETLVTTGQVHVAGEV
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECC
TTSAYADIPRIVREKVLEIGYDSSAKGFDGNSCGVNIAIGAQSPDIAQGVDTSHEARVGG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCC
TDDEIAKQGAGDQGLMFGYATTDTPELMPLPIALAHRLSRKLTEVRKTGVLPYLRPDGKT
CHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCE
QVTIEYDGDRPVRLDTVVISTQHAADIDLDNLLAPDIREKVVDAVLADLDLPSPLDTSDI
EEEEEECCCCCEEEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCE
RLLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRW
EEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHH
VAKNVVAAGLSERVEVQVAYAIGKAAPVGLFVETFGTEKVDPARIAAAITEVFDLRPGAI
HHHHHHHHCCCHHEEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCH
IRDLDLLRPIYAPTAAYGHFGRTDIDLPWEHTDRADKLRAAAGL
HHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA