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The map label for this gene is priA [H]

Identifier: 54025582

GI number: 54025582

Start: 3822452

End: 3824533

Strand: Reverse

Name: priA [H]

Synonym: nfa36120

Alternate gene names: 54025582

Gene position: 3824533-3822452 (Counterclockwise)

Preceding gene: 54025583

Following gene: 54025581

Centisome position: 63.52

GC content: 75.98

Gene sequence:

>2082_bases
GTGCTGCCGCTGCTCACGCCCGCCCACCTCGACCGCGACTTCGACTATCTCGTCCCCGCCGAGATGGACGAGCTCGCCCG
GCCCGGCGTGCGCGTGCGGGTGCGGTTCGCGGGGCGTCTGGTCGACGGGTACCTGCTGGACCGCCTCGAGCGCAGCGACC
ACGATGGCACCCTCATCCCGCTCGAGCGCGTGGTCTCCGGCGAGCGGGTGCTCACCCCGGAAATCCTGCGATTGGTCACC
GCCGTGGCGAGCCGGTACGCGGGTACCCGCGCCGACGTGCTGCGGCTGGCGATCCCGCCGCGACATGCGCGCGCCGAGAA
CGGGGGAGCGAAGAAGGCTGCGGGCCAAGGCGATTCGGCCGCGGGCGGGCCGGAAGCCGCCGCTGCGGACGTGCCACCCA
TCGATGCCGACGGGGCGGAGGCCGAGGTCTCGGGCTCGTCCGTCGCGACATCCGTTGGCCCGGAAGCTGATTCGGCCGAA
CGTCCCCCGGTCGACACGGCCGGGTGGGCGCGCTACCTGCACGGCGACTCCTTCGTCGCCGCACTCGCGGCGGGTCGCGG
CGTGCGGGCGGCCTGGCAGGCGCTGCCGGGGGAGGACTGGGCGCGCAGGCTGGCCGAGTTGGCGGCGGTGGTGGTGCGGT
CGGGACGCGGCGTGATCCTCATGGTGCCCGACCAGCGCGACCTCGATCGGGTGCTGGCGGAATGCGTTGCGCTGGTGGGG
GATACGGCGGTGGGATTGTCGGCAGGGCTCGGGCCCGCGGCCAGGTACCGGCGCTGGCTGGCGGTGCTGCGCGGTCAGGC
CAGGGTGGTGGTGGGGACGCGGGCGGCGGTGTTCGCTCCGGTCGCCGACCTCGGTCTCGTCGCGATCTGGGACGACGGTG
ACGACACCTACGCCGAGCCGCGCGCACCCTATCCGCACGCGCGGGAGGTCTCGATGCTGCGTGCGCACGAGACCGGCGCG
GCGTTCGTGGCGGCCGGATTCGCGCGCACCGCCGAGGTGCAGGCGGTGGTCGATTCGGGGTGGGCCCACGATCTGGTCGC
CGAACGGCCGGTGGTCCGCACGGCGGCGCCGCGGATCAGCGCGCCGGGGGACAGCGACATCGCCCTCGAACGCGATCCGG
TGGCGCGCGCGGTGCGGATTCCGGCGGTGGCGTTCGCGGCCGCCCGGGCGGCGCTGAAGGAAGGCGCCGCGGTGCTGGTG
CAGGTGCCGCGACGCGGTTACGTGCCCGCGTTGGCCTGCTCGAAATGCCGGACCCCCGCCCGCTGCCGGCGCTGCAACGG
GCCACTGGCGCTGCCCGACGCGCCCGACGGCGCGGCCCACAGTCCGGCCTGCCGCTGGTGCGGCGTCGCCGAGACCGCGT
TCCGCTGCCCGGTGTGCGCCTCGCGGGCGCTGCGCGCGGTGGTCATCGGCGCCACCCGCACCGCCGAAGAACTGGGCCGG
GCCTTTCCCGGCGTGCCGATCCGCGGGTCGAGCGCCGGGGCGATGCTCGACAGCGTGGAACCGGGGCCGCAGGTCGTGGT
GGCGACGGTCGGCGCCGAACCGGTGGTGCGCGGCGGGTACGGCGTCGCGCTGCTGCTGGACGGGTGGGCACTGCTCGGCC
GCGCCGATCTGCGGGCCGGCGAGGATGCGCTGCGCCGATGGCTGAACGCCGCCGCGTTGGTGCGCTCGCACGGCCAGGTG
GTCGTGATGGCCGAGCCCGCCCTGCCGACGGTGCAGGCGCTGGTGCGCTGGGATCCGGTCGGACATGCGCGTGCGGAGGT
GGCCGAGCGGTCCGAGGTCGGTTTCCCGCCCGCCGTGCGACTCGCGGCGGTCGACGGCGCGGCCGAGGCGATCCGGGATC
TGCTGGCCGCCGCCACGCTGCCCGACGGGGTGGAGGTGCTCGGCCCCGTGCCGTTGCCCGAGGGCGCCCGCAAGCCGTTC
GCCGGTGATTCGCCCGCCGAGGTCGAGCGGGTGTTGCTGCGCGTCGACCGGCGAGCCGGGGCGGCGCTGGCACGCGCGCT
CACCGCCGCGCAGGCGGTGCGCAGCACCCACCGGTCCGACGCGCCGCTGCGGGTGCAGATCGATCCCGTCGACATCGGCT
GA

Upstream 100 bases:

>100_bases
GGCAGCCCCCGCGGACACCGCGTCCGCGGGGGCGTCGGCGTCTTCGGCGCGCCGTCGCAAGCCGCGTGGCGCCCCGGCGG
CCACCCTGCCGATCGCGCGG

Downstream 100 bases:

>100_bases
CCCGCGCGGCGGGCCCGGGGGTTTCGGTCCGGTCAGACGGGCAACCCGATCACTGGCCGATCGCACACCCGGTGTGCTCG
GCGCGCCCAGGGCAGACCGC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MLPLLTPAHLDRDFDYLVPAEMDELARPGVRVRVRFAGRLVDGYLLDRLERSDHDGTLIPLERVVSGERVLTPEILRLVT
AVASRYAGTRADVLRLAIPPRHARAENGGAKKAAGQGDSAAGGPEAAAADVPPIDADGAEAEVSGSSVATSVGPEADSAE
RPPVDTAGWARYLHGDSFVAALAAGRGVRAAWQALPGEDWARRLAELAAVVVRSGRGVILMVPDQRDLDRVLAECVALVG
DTAVGLSAGLGPAARYRRWLAVLRGQARVVVGTRAAVFAPVADLGLVAIWDDGDDTYAEPRAPYPHAREVSMLRAHETGA
AFVAAGFARTAEVQAVVDSGWAHDLVAERPVVRTAAPRISAPGDSDIALERDPVARAVRIPAVAFAAARAALKEGAAVLV
QVPRRGYVPALACSKCRTPARCRRCNGPLALPDAPDGAAHSPACRWCGVAETAFRCPVCASRALRAVVIGATRTAEELGR
AFPGVPIRGSSAGAMLDSVEPGPQVVVATVGAEPVVRGGYGVALLLDGWALLGRADLRAGEDALRRWLNAAALVRSHGQV
VVMAEPALPTVQALVRWDPVGHARAEVAERSEVGFPPAVRLAAVDGAAEAIRDLLAAATLPDGVEVLGPVPLPEGARKPF
AGDSPAEVERVLLRVDRRAGAALARALTAAQAVRSTHRSDAPLRVQIDPVDIG

Sequences:

>Translated_693_residues
MLPLLTPAHLDRDFDYLVPAEMDELARPGVRVRVRFAGRLVDGYLLDRLERSDHDGTLIPLERVVSGERVLTPEILRLVT
AVASRYAGTRADVLRLAIPPRHARAENGGAKKAAGQGDSAAGGPEAAAADVPPIDADGAEAEVSGSSVATSVGPEADSAE
RPPVDTAGWARYLHGDSFVAALAAGRGVRAAWQALPGEDWARRLAELAAVVVRSGRGVILMVPDQRDLDRVLAECVALVG
DTAVGLSAGLGPAARYRRWLAVLRGQARVVVGTRAAVFAPVADLGLVAIWDDGDDTYAEPRAPYPHAREVSMLRAHETGA
AFVAAGFARTAEVQAVVDSGWAHDLVAERPVVRTAAPRISAPGDSDIALERDPVARAVRIPAVAFAAARAALKEGAAVLV
QVPRRGYVPALACSKCRTPARCRRCNGPLALPDAPDGAAHSPACRWCGVAETAFRCPVCASRALRAVVIGATRTAEELGR
AFPGVPIRGSSAGAMLDSVEPGPQVVVATVGAEPVVRGGYGVALLLDGWALLGRADLRAGEDALRRWLNAAALVRSHGQV
VVMAEPALPTVQALVRWDPVGHARAEVAERSEVGFPPAVRLAAVDGAAEAIRDLLAAATLPDGVEVLGPVPLPEGARKPF
AGDSPAEVERVLLRVDRRAGAALARALTAAQAVRSTHRSDAPLRVQIDPVDIG
>Mature_693_residues
MLPLLTPAHLDRDFDYLVPAEMDELARPGVRVRVRFAGRLVDGYLLDRLERSDHDGTLIPLERVVSGERVLTPEILRLVT
AVASRYAGTRADVLRLAIPPRHARAENGGAKKAAGQGDSAAGGPEAAAADVPPIDADGAEAEVSGSSVATSVGPEADSAE
RPPVDTAGWARYLHGDSFVAALAAGRGVRAAWQALPGEDWARRLAELAAVVVRSGRGVILMVPDQRDLDRVLAECVALVG
DTAVGLSAGLGPAARYRRWLAVLRGQARVVVGTRAAVFAPVADLGLVAIWDDGDDTYAEPRAPYPHAREVSMLRAHETGA
AFVAAGFARTAEVQAVVDSGWAHDLVAERPVVRTAAPRISAPGDSDIALERDPVARAVRIPAVAFAAARAALKEGAAVLV
QVPRRGYVPALACSKCRTPARCRRCNGPLALPDAPDGAAHSPACRWCGVAETAFRCPVCASRALRAVVIGATRTAEELGR
AFPGVPIRGSSAGAMLDSVEPGPQVVVATVGAEPVVRGGYGVALLLDGWALLGRADLRAGEDALRRWLNAAALVRSHGQV
VVMAEPALPTVQALVRWDPVGHARAEVAERSEVGFPPAVRLAAVDGAAEAIRDLLAAATLPDGVEVLGPVPLPEGARKPF
AGDSPAEVERVLLRVDRRAGAALARALTAAQAVRSTHRSDAPLRVQIDPVDIG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=702, Percent_Identity=25.7834757834758, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 72353; Mature: 72353

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLLTPAHLDRDFDYLVPAEMDELARPGVRVRVRFAGRLVDGYLLDRLERSDHDGTLIP
CCCCCCHHHCCCCCCEECCCCHHHHHCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCEEE
LERVVSGERVLTPEILRLVTAVASRYAGTRADVLRLAIPPRHARAENGGAKKAAGQGDSA
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCC
AGGPEAAAADVPPIDADGAEAEVSGSSVATSVGPEADSAERPPVDTAGWARYLHGDSFVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHH
ALAAGRGVRAAWQALPGEDWARRLAELAAVVVRSGRGVILMVPDQRDLDRVLAECVALVG
HHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHC
DTAVGLSAGLGPAARYRRWLAVLRGQARVVVGTRAAVFAPVADLGLVAIWDDGDDTYAEP
CCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCEEEEEECCCCCCCCC
RAPYPHAREVSMLRAHETGAAFVAAGFARTAEVQAVVDSGWAHDLVAERPVVRTAAPRIS
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHCCCCCC
APGDSDIALERDPVARAVRIPAVAFAAARAALKEGAAVLVQVPRRGYVPALACSKCRTPA
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCH
RCRRCNGPLALPDAPDGAAHSPACRWCGVAETAFRCPVCASRALRAVVIGATRTAEELGR
HHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHCHHHHHHHHHEEEEECCCCHHHHHH
AFPGVPIRGSSAGAMLDSVEPGPQVVVATVGAEPVVRGGYGVALLLDGWALLGRADLRAG
HCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCEEECCCCEEEEECCHHHHHHHHCCCC
EDALRRWLNAAALVRSHGQVVVMAEPALPTVQALVRWDPVGHARAEVAERSEVGFPPAVR
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEE
LAAVDGAAEAIRDLLAAATLPDGVEVLGPVPLPEGARKPFAGDSPAEVERVLLRVDRRAG
EEECCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AALARALTAAQAVRSTHRSDAPLRVQIDPVDIG
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MLPLLTPAHLDRDFDYLVPAEMDELARPGVRVRVRFAGRLVDGYLLDRLERSDHDGTLIP
CCCCCCHHHCCCCCCEECCCCHHHHHCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCEEE
LERVVSGERVLTPEILRLVTAVASRYAGTRADVLRLAIPPRHARAENGGAKKAAGQGDSA
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCC
AGGPEAAAADVPPIDADGAEAEVSGSSVATSVGPEADSAERPPVDTAGWARYLHGDSFVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHH
ALAAGRGVRAAWQALPGEDWARRLAELAAVVVRSGRGVILMVPDQRDLDRVLAECVALVG
HHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHC
DTAVGLSAGLGPAARYRRWLAVLRGQARVVVGTRAAVFAPVADLGLVAIWDDGDDTYAEP
CCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCEEEEEECCCCCCCCC
RAPYPHAREVSMLRAHETGAAFVAAGFARTAEVQAVVDSGWAHDLVAERPVVRTAAPRIS
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHCCCCCC
APGDSDIALERDPVARAVRIPAVAFAAARAALKEGAAVLVQVPRRGYVPALACSKCRTPA
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCH
RCRRCNGPLALPDAPDGAAHSPACRWCGVAETAFRCPVCASRALRAVVIGATRTAEELGR
HHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHCHHHHHHHHHEEEEECCCCHHHHHH
AFPGVPIRGSSAGAMLDSVEPGPQVVVATVGAEPVVRGGYGVALLLDGWALLGRADLRAG
HCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCEEECCCCEEEEECCHHHHHHHHCCCC
EDALRRWLNAAALVRSHGQVVVMAEPALPTVQALVRWDPVGHARAEVAERSEVGFPPAVR
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEE
LAAVDGAAEAIRDLLAAATLPDGVEVLGPVPLPEGARKPFAGDSPAEVERVLLRVDRRAG
EEECCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AALARALTAAQAVRSTHRSDAPLRVQIDPVDIG
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]