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The map label for this gene is shiA [H]

Identifier: 54025513

GI number: 54025513

Start: 3754633

End: 3755964

Strand: Reverse

Name: shiA [H]

Synonym: nfa35430

Alternate gene names: 54025513

Gene position: 3755964-3754633 (Counterclockwise)

Preceding gene: 54025514

Following gene: 54025512

Centisome position: 62.38

GC content: 67.27

Gene sequence:

>1332_bases
ATGACCCCTCGGACCGACAGCGTCGCGGTCTCCGCCACCCCCGCCCAGGCGCCCGCCAGACCGGGCCGTGCCGCACTGGC
CGCAGCCATCTCGACCTCGCTCGAGTGGTACGACTTCTTCATCTATTCGACCGCTGCCGCACTGGTGTTCAATTCGACCT
TCTTCGCCACCGGTGACGAGGTCGTCGCGGCGGTCAACTCGTTCGCCACGGTGGCCGTCGGCTTCATCGCCCGCCCGATC
GGCGGTGTCCTCGCCGGGCATTTCGGCGACAAGATCGGACGCAAACCGGTGCTGGTCACCGCCGTGGTGCTGATGGCGGT
CGCGACCGCGCTGATCGGGTTCGTGCCCAACACCGAGATCGTCTGGCTCGCGCCGGTGATCCTGGTGACGCTGCGCATCT
GCCAGGGTCTCGCGGTCGGCGCGCAGTGGGGCGGGGCGGTGCTGCTGGCCACCGAGAACGCGCCCGCGCATCGGCGCGGA
TTCTTCGGCAGCTTCGCGCAATTGGGTGTGCCGATCGGTGTGGTGTTCGGCAATGTGGCGTTCCTGGTGATCACCGCGAC
GGTCGCCAAGGATCAGTTCCTCACCTGGGGTTGGCGTATTCCGTTCTGGATCAGCCTGGTGATGTTGCCGGTGGCCTTCG
CCATCTACCGCTATCTGGAGGAGACACCCGAGTTCCAGAAGGTCGCCGAGAAGCTCGACACCGCCCCGAAAGCGCCCCGC
TCACCGATCTTCGAGGTCCTGCGCCGCAACCCCGGCACCGTGCTGATGGCGGCGGGCTCCAACGCGATCGGAATCATCTT
CTTCTACACGATGATCACCGGGTCGGTGCAGTTCGCCACCACCTACCTGGACATGGCGCGCTCGACCGTCCTGACCTACA
TCCTCATCGCCTGCTCGATCCAGGTGCCGCTGGTCCCGCTGTTCGGGTATCTGTCCGACCGGGTGGGGCGCAAGCTGATC
TTCGGTGTCGGCACCGCGGCGATGATGCTGTGGGGCGTGCCGATGTGGCTGCTGATCGGCGCGTCCGGGCCCGGCACAGT
GTGGCCGTTCGCGGTCGCGGTGATCGTCGGCGTGGTCGCGATGTCGTTCCAGACCGGCACCCAGGGCACGCTGTTCTCGG
AACTGTTCCCGCCCGAGGTGCGGTTCTCCGGTGCCTCGCTCGGCTACCAGATCTCGGCCATCATCGGTGGCTTCGCGCCG
ATGATCATGGTGCTGCTCATCAACGGCAAACCCGGCAACGCCTGGCGCGTCGGCGCCTTCCTCGCCGTCGCCGGTCTGGT
GGGCCTGCTGTGCCTGCTGGCCATCGTCCGCCGCTACTCGCGTGCCCGCTGA

Upstream 100 bases:

>100_bases
CGAACTCGCCGCCACCCCGGACCTCCGCGAGACCCCCTCCTGAATCACCCGGCATCCCAGGCGATGCCGCCGTTCGCCCC
ATCCAGAATCGAGTACAGCC

Downstream 100 bases:

>100_bases
ACTCGAGGACATCGCCATGACGGATTTCCCGGACACCCACGCCGCCGCCGACCCGGACCGCCCGGCCTACATCATGGCCG
AGACGGACACGGTGGTGACC

Product: putative transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTPRTDSVAVSATPAQAPARPGRAALAAAISTSLEWYDFFIYSTAAALVFNSTFFATGDEVVAAVNSFATVAVGFIARPI
GGVLAGHFGDKIGRKPVLVTAVVLMAVATALIGFVPNTEIVWLAPVILVTLRICQGLAVGAQWGGAVLLATENAPAHRRG
FFGSFAQLGVPIGVVFGNVAFLVITATVAKDQFLTWGWRIPFWISLVMLPVAFAIYRYLEETPEFQKVAEKLDTAPKAPR
SPIFEVLRRNPGTVLMAAGSNAIGIIFFYTMITGSVQFATTYLDMARSTVLTYILIACSIQVPLVPLFGYLSDRVGRKLI
FGVGTAAMMLWGVPMWLLIGASGPGTVWPFAVAVIVGVVAMSFQTGTQGTLFSELFPPEVRFSGASLGYQISAIIGGFAP
MIMVLLINGKPGNAWRVGAFLAVAGLVGLLCLLAIVRRYSRAR

Sequences:

>Translated_443_residues
MTPRTDSVAVSATPAQAPARPGRAALAAAISTSLEWYDFFIYSTAAALVFNSTFFATGDEVVAAVNSFATVAVGFIARPI
GGVLAGHFGDKIGRKPVLVTAVVLMAVATALIGFVPNTEIVWLAPVILVTLRICQGLAVGAQWGGAVLLATENAPAHRRG
FFGSFAQLGVPIGVVFGNVAFLVITATVAKDQFLTWGWRIPFWISLVMLPVAFAIYRYLEETPEFQKVAEKLDTAPKAPR
SPIFEVLRRNPGTVLMAAGSNAIGIIFFYTMITGSVQFATTYLDMARSTVLTYILIACSIQVPLVPLFGYLSDRVGRKLI
FGVGTAAMMLWGVPMWLLIGASGPGTVWPFAVAVIVGVVAMSFQTGTQGTLFSELFPPEVRFSGASLGYQISAIIGGFAP
MIMVLLINGKPGNAWRVGAFLAVAGLVGLLCLLAIVRRYSRAR
>Mature_442_residues
TPRTDSVAVSATPAQAPARPGRAALAAAISTSLEWYDFFIYSTAAALVFNSTFFATGDEVVAAVNSFATVAVGFIARPIG
GVLAGHFGDKIGRKPVLVTAVVLMAVATALIGFVPNTEIVWLAPVILVTLRICQGLAVGAQWGGAVLLATENAPAHRRGF
FGSFAQLGVPIGVVFGNVAFLVITATVAKDQFLTWGWRIPFWISLVMLPVAFAIYRYLEETPEFQKVAEKLDTAPKAPRS
PIFEVLRRNPGTVLMAAGSNAIGIIFFYTMITGSVQFATTYLDMARSTVLTYILIACSIQVPLVPLFGYLSDRVGRKLIF
GVGTAAMMLWGVPMWLLIGASGPGTVWPFAVAVIVGVVAMSFQTGTQGTLFSELFPPEVRFSGASLGYQISAIIGGFAPM
IMVLLINGKPGNAWRVGAFLAVAGLVGLLCLLAIVRRYSRAR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=442, Percent_Identity=34.3891402714932, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1789941, Length=428, Percent_Identity=36.9158878504673, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1790550, Length=415, Percent_Identity=29.8795180722892, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1788942, Length=416, Percent_Identity=30.2884615384615, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87082231, Length=232, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082404, Length=429, Percent_Identity=24.9417249417249, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47065; Mature: 46934

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPRTDSVAVSATPAQAPARPGRAALAAAISTSLEWYDFFIYSTAAALVFNSTFFATGDE
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEECCCHH
VVAAVNSFATVAVGFIARPIGGVLAGHFGDKIGRKPVLVTAVVLMAVATALIGFVPNTEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCH
VWLAPVILVTLRICQGLAVGAQWGGAVLLATENAPAHRRGFFGSFAQLGVPIGVVFGNVA
HHHHHHHHHHHHHHCCCEECCCCCCEEEEEECCCCCHHHCCCCCHHHHCCHHHHHHHHHH
FLVITATVAKDQFLTWGWRIPFWISLVMLPVAFAIYRYLEETPEFQKVAEKLDTAPKAPR
HHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
SPIFEVLRRNPGTVLMAAGSNAIGIIFFYTMITGSVQFATTYLDMARSTVLTYILIACSI
CHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
QVPLVPLFGYLSDRVGRKLIFGVGTAAMMLWGVPMWLLIGASGPGTVWPFAVAVIVGVVA
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHH
MSFQTGTQGTLFSELFPPEVRFSGASLGYQISAIIGGFAPMIMVLLINGKPGNAWRVGAF
HHCCCCCCCCHHHHCCCCCCEECCCCCCEEHHHHHHHHHHHHHHHEEECCCCCCCHHHHH
LAVAGLVGLLCLLAIVRRYSRAR
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPRTDSVAVSATPAQAPARPGRAALAAAISTSLEWYDFFIYSTAAALVFNSTFFATGDE
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEECCCHH
VVAAVNSFATVAVGFIARPIGGVLAGHFGDKIGRKPVLVTAVVLMAVATALIGFVPNTEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCH
VWLAPVILVTLRICQGLAVGAQWGGAVLLATENAPAHRRGFFGSFAQLGVPIGVVFGNVA
HHHHHHHHHHHHHHCCCEECCCCCCEEEEEECCCCCHHHCCCCCHHHHCCHHHHHHHHHH
FLVITATVAKDQFLTWGWRIPFWISLVMLPVAFAIYRYLEETPEFQKVAEKLDTAPKAPR
HHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
SPIFEVLRRNPGTVLMAAGSNAIGIIFFYTMITGSVQFATTYLDMARSTVLTYILIACSI
CHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
QVPLVPLFGYLSDRVGRKLIFGVGTAAMMLWGVPMWLLIGASGPGTVWPFAVAVIVGVVA
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHH
MSFQTGTQGTLFSELFPPEVRFSGASLGYQISAIIGGFAPMIMVLLINGKPGNAWRVGAF
HHCCCCCCCCHHHHCCCCCCEECCCCCCEEHHHHHHHHHHHHHHHEEECCCCCCCHHHHH
LAVAGLVGLLCLLAIVRRYSRAR
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]