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The map label for this gene is phnD [H]

Identifier: 54025500

GI number: 54025500

Start: 3742488

End: 3743453

Strand: Reverse

Name: phnD [H]

Synonym: nfa35300

Alternate gene names: 54025500

Gene position: 3743453-3742488 (Counterclockwise)

Preceding gene: 54025501

Following gene: 54025499

Centisome position: 62.17

GC content: 69.36

Gene sequence:

>966_bases
ATGAACATCCGCCGCTCCCGCCCGCTCGTCTCGCTGCTGGCCGGATTCGCCCTCGCGCTGACGGCCTGTTCCGGCTCCGG
CGCGGACTCCCGCGCGATCAACGACAGGGGCTTCCCCGAGACCCTGACCCTGGCCGCCATCCCGGCCGAGAACTCGACCG
ACCTGAAGGCCAGCTACGATCCCGTCATCGCGCTGCTCGAACGCGAGACCGGCGCGAAGATCGACTTCGTGCAGGCGTCC
GACTACGCGGGCGTGGTCGAGGGCATCATCGCGGGCAACGTCGACATGGCGTTCTTCGGCCCGTTCGCCTACGTCGTCGC
CGGTGTCAACGGCGCGAAGATGACCCCGCTGGGTGCGGTCGTACAGGAACCCGGGGGCAAGCCCGGCTATCAGTCCTACG
GCCTGGCCCGTTCCGACAACGCCGTGATCGAGTCGTTGGCCGACTTCGCGGGCAAGAAGGTGTGTTTCGTCGATCCCAGC
TCGACCTCCGGATTCCTCTATCCCACCGCGGGTCTGATCGAGCAGAAGGTCATCGCCTCCGGCGCGGAGGCCGACCTGTC
CAAGGGGCTGACGCCGATCTACGCGGGCGGGCACGACGCCTCGGCGCTCTCGATCGCCGCGGGTGACTGCGACGCGGGCT
TCGCCTTCGACACCATGGTCGACAAGACCCTCGTCGAGAAGGGCGACTTGGCGCCGGGCCAGTTGAAGACGGTGTGGAAG
TCCGAGACCATCGCGGGCTCGGTGTTCGCCGCCGCCGACGCGCTGGGCGCGGAGACCGTCGCGAAACTGCGGACGCTGTT
CACCGAGAAGGTGAACGCCGACCACCTGCGCGGCGCCGGCCTGTGCACGGGCGAGTGCCGGATCACCGACGAACGCGCCT
GGGGCGTGGTGCCCGCCGCCGACAGCGACTACGACGGCGTGCGGCACGTCTGCGAGGTCACCCGCTCCGACAAGTGCGCG
GGCTGA

Upstream 100 bases:

>100_bases
GCTGCGCCGCGCCGGGGCCACCCACGTGCTCGACTCCGTCGCCGAACTGCCCGCCCTGTTGCTGCCCGCCTGAAACCTCT
CCCACCGCAAGGAACTTCGC

Downstream 100 bases:

>100_bases
GCGACGTGTCCGCCGATTCCCGCGCCGAGGTGGTGCTGCACGCCGAGGCGGTGACCAAGCGCTTCGGTGCCACCCTGGCG
CTCGATGCGGTATCGATGAC

Product: putative transporter

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNIRRSRPLVSLLAGFALALTACSGSGADSRAINDRGFPETLTLAAIPAENSTDLKASYDPVIALLERETGAKIDFVQAS
DYAGVVEGIIAGNVDMAFFGPFAYVVAGVNGAKMTPLGAVVQEPGGKPGYQSYGLARSDNAVIESLADFAGKKVCFVDPS
STSGFLYPTAGLIEQKVIASGAEADLSKGLTPIYAGGHDASALSIAAGDCDAGFAFDTMVDKTLVEKGDLAPGQLKTVWK
SETIAGSVFAAADALGAETVAKLRTLFTEKVNADHLRGAGLCTGECRITDERAWGVVPAADSDYDGVRHVCEVTRSDKCA
G

Sequences:

>Translated_321_residues
MNIRRSRPLVSLLAGFALALTACSGSGADSRAINDRGFPETLTLAAIPAENSTDLKASYDPVIALLERETGAKIDFVQAS
DYAGVVEGIIAGNVDMAFFGPFAYVVAGVNGAKMTPLGAVVQEPGGKPGYQSYGLARSDNAVIESLADFAGKKVCFVDPS
STSGFLYPTAGLIEQKVIASGAEADLSKGLTPIYAGGHDASALSIAAGDCDAGFAFDTMVDKTLVEKGDLAPGQLKTVWK
SETIAGSVFAAADALGAETVAKLRTLFTEKVNADHLRGAGLCTGECRITDERAWGVVPAADSDYDGVRHVCEVTRSDKCA
G
>Mature_321_residues
MNIRRSRPLVSLLAGFALALTACSGSGADSRAINDRGFPETLTLAAIPAENSTDLKASYDPVIALLERETGAKIDFVQAS
DYAGVVEGIIAGNVDMAFFGPFAYVVAGVNGAKMTPLGAVVQEPGGKPGYQSYGLARSDNAVIESLADFAGKKVCFVDPS
STSGFLYPTAGLIEQKVIASGAEADLSKGLTPIYAGGHDASALSIAAGDCDAGFAFDTMVDKTLVEKGDLAPGQLKTVWK
SETIAGSVFAAADALGAETVAKLRTLFTEKVNADHLRGAGLCTGECRITDERAWGVVPAADSDYDGVRHVCEVTRSDKCA
G

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for phosphate binding [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790543, Length=230, Percent_Identity=27.8260869565217, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 33171; Mature: 33171

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRRSRPLVSLLAGFALALTACSGSGADSRAINDRGFPETLTLAAIPAENSTDLKASYD
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
PVIALLERETGAKIDFVQASDYAGVVEGIIAGNVDMAFFGPFAYVVAGVNGAKMTPLGAV
CEEEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
VQEPGGKPGYQSYGLARSDNAVIESLADFAGKKVCFVDPSSTSGFLYPTAGLIEQKVIAS
HHCCCCCCCCHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEECCHHHHHHHHHHC
GAEADLSKGLTPIYAGGHDASALSIAAGDCDAGFAFDTMVDKTLVEKGDLAPGQLKTVWK
CCHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHC
SETIAGSVFAAADALGAETVAKLRTLFTEKVNADHLRGAGLCTGECRITDERAWGVVPAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEECCCEEEECCCCCEEECCC
DSDYDGVRHVCEVTRSDKCAG
CCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNIRRSRPLVSLLAGFALALTACSGSGADSRAINDRGFPETLTLAAIPAENSTDLKASYD
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
PVIALLERETGAKIDFVQASDYAGVVEGIIAGNVDMAFFGPFAYVVAGVNGAKMTPLGAV
CEEEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
VQEPGGKPGYQSYGLARSDNAVIESLADFAGKKVCFVDPSSTSGFLYPTAGLIEQKVIAS
HHCCCCCCCCHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEECCHHHHHHHHHHC
GAEADLSKGLTPIYAGGHDASALSIAAGDCDAGFAFDTMVDKTLVEKGDLAPGQLKTVWK
CCHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHC
SETIAGSVFAAADALGAETVAKLRTLFTEKVNADHLRGAGLCTGECRITDERAWGVVPAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEECCCEEEECCCCCEEECCC
DSDYDGVRHVCEVTRSDKCAG
CCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA