Click here to switch to the map view.

The map label for this gene is rlmB [H]

Identifier: 54020641

GI number: 54020641

Start: 832832

End: 833533

Strand: Reverse

Name: rlmB [H]

Synonym: mhp660

Alternate gene names: 54020641

Gene position: 833533-832832 (Counterclockwise)

Preceding gene: 54020642

Following gene: 54020640

Centisome position: 93.37

GC content: 28.63

Gene sequence:

>702_bases
ATGGTTAAATATATTTGTGGCAGAAATTCATTAGCCGACGCGATCAAGAATGGGTTTATTTTTAAGGAAATTTACCTTTT
AAGACCTACAAATGATCAAATTTTCTTAAAACAAAAAGTAAAAATAGTTAGTCGTGATTTTTTAGATAAACTTGTCAGCC
TGAATCACCAAGGTTTTGTTGGTGTTCTTGAGAATTTTAAATTTTTTGAAATTGATACATTAGTAAAAGATAAACCAAAA
ATTATTTTGATCCTTGATCATATCCAGGACCAAAATAATCTTGGAAATATCATTAGAACTGCAAATTGTTTTGGAATTAA
GCATATAATTTTACCTAAAAAGCGAGCAGCAAAATTGAATGAGACCACTATGAAAGTCTCATCTGGTGGTTTTGTCGGCC
TTAAATTTATTTTAGTTAATTCCATTGTTTCAGCAATTAATAAATTAAAAGAGATGGATTTTTGAATTTATGCCACTGAT
CTTAAAGAAAAAAGTAGAAAAATCCAGGAAATTCAGTTTAATTATCCTTGCGCAATAATTTTAGGTAATGAGGGAACTGG
AATCAAAAAAAGCAGTTTAAATGCAAGTGATCAAACTTTTTTTATCCCGCTTGAAGGAACAGTTAACTCGCTAAATGTGA
CAGTTGCGACCGGAATTATTCTTTTTTTTCTAAAACAAAATATAAAAAAAACAAAAATATAA

Upstream 100 bases:

>100_bases
GGATAAAAACTATTCCGAGGCCGATAAAATCCGTAAAGAATTATGAAAAATATTTAAAAATTCATAATTTTTTCCATCTT
CCTTTTGAAAGATTGAAAGT

Downstream 100 bases:

>100_bases
AGGGAAAAAATGTGTTTTCTATGTCACCAGAGCGAAAAGAAAAAATAAAAAAAAATAAACGCTGTTTAAAGGTTTTAAAT
AAATATTCACATATTTTATT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MVKYICGRNSLADAIKNGFIFKEIYLLRPTNDQIFLKQKVKIVSRDFLDKLVSLNHQGFVGVLENFKFFEIDTLVKDKPK
IILILDHIQDQNNLGNIIRTANCFGIKHIILPKKRAAKLNETTMKVSSGGFVGLKFILVNSIVSAINKLKEMDFWIYATD
LKEKSRKIQEIQFNYPCAIILGNEGTGIKKSSLNASDQTFFIPLEGTVNSLNVTVATGIILFFLKQNIKKTKI

Sequences:

>Translated_233_residues
MVKYICGRNSLADAIKNGFIFKEIYLLRPTNDQIFLKQKVKIVSRDFLDKLVSLNHQGFVGVLENFKFFEIDTLVKDKPK
IILILDHIQDQNNLGNIIRTANCFGIKHIILPKKRAAKLNETTMKVSSGGFVGLKFILVNSIVSAINKLKEMDF*IYATD
LKEKSRKIQEIQFNYPCAIILGNEGTGIKKSSLNASDQTFFIPLEGTVNSLNVTVATGIILFFLKQNIKKTKI
>Mature_233_residues
MVKYICGRNSLADAIKNGFIFKEIYLLRPTNDQIFLKQKVKIVSRDFLDKLVSLNHQGFVGVLENFKFFEIDTLVKDKPK
IILILDHIQDQNNLGNIIRTANCFGIKHIILPKKRAAKLNETTMKVSSGGFVGLKFILVNSIVSAINKLKEMDF*IYATD
LKEKSRKIQEIQFNYPCAIILGNEGTGIKKSSLNASDQTFFIPLEGTVNSLNVTVATGIILFFLKQNIKKTKI

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=216, Percent_Identity=30.0925925925926, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790083, Length=153, Percent_Identity=30.0653594771242, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26182; Mature: 26182

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKYICGRNSLADAIKNGFIFKEIYLLRPTNDQIFLKQKVKIVSRDFLDKLVSLNHQGFV
CEEEEECCCHHHHHHHCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHH
GVLENFKFFEIDTLVKDKPKIILILDHIQDQNNLGNIIRTANCFGIKHIILPKKRAAKLN
HHHCCCEEEEEEHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHHCCCC
ETTMKVSSGGFVGLKFILVNSIVSAINKLKEMDFIYATDLKEKSRKIQEIQFNYPCAIIL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHEEEECCCEEEEE
GNEGTGIKKSSLNASDQTFFIPLEGTVNSLNVTVATGIILFFLKQNIKKTKI
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKYICGRNSLADAIKNGFIFKEIYLLRPTNDQIFLKQKVKIVSRDFLDKLVSLNHQGFV
CEEEEECCCHHHHHHHCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHH
GVLENFKFFEIDTLVKDKPKIILILDHIQDQNNLGNIIRTANCFGIKHIILPKKRAAKLN
HHHCCCEEEEEEHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHHCCCC
ETTMKVSSGGFVGLKFILVNSIVSAINKLKEMDFIYATDLKEKSRKIQEIQFNYPCAIIL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHEEEECCCEEEEE
GNEGTGIKKSSLNASDQTFFIPLEGTVNSLNVTVATGIILFFLKQNIKKTKI
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA