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The map label for this gene is nagE

Identifier: 54020606

GI number: 54020606

Start: 740648

End: 742429

Strand: Reverse

Name: nagE

Synonym: mhp590

Alternate gene names: 54020606

Gene position: 742429-740648 (Counterclockwise)

Preceding gene: 54020607

Following gene: 54020605

Centisome position: 83.16

GC content: 33.67

Gene sequence:

>1782_bases
ATGTCAGTAGGATTTGTAAAAAAAATAAAGTTTTTTCGGCCAAAAAAGGCCGAAAATTATAGTCAAAATTCGATTTTTAA
CCGAATTTTGAATCAATTACAGCGGCTTGGTAGATCACTAATGTTCCCAATTGCCGTTTTACCGATTGCGGCACTTTTAT
TACGAATTGGTGCACTAATTCAGGATCCTTTAGCAAATGGATCAATTGGAATATCCGAGACGCAAAAATTTTGGGGAAGG
CTGATTTCAACCCCAGGCGGAATAGTTTTTGATAATCTAGCGATAATTTTTGCAATTGGAATCGGTTTTGGTTTTGCCAA
AGACAACCGTGGCGAGGCCGCTCTTGTAGCTGCTGTTGTTTGATATGGGATGTCAACACTTCTAAAACAGAACCAATTAG
CGGAGGCAATTTATAAAAATGTTTTAGTTGCTAAGTCCGGTCAACTCAAAGGATTTACGCAACTTTTGTATTTTTTAAAA
GCAGGAAAGCCAGTTTATCAGCTCGATTCAGGAGTTATTGGCGGAATTAGTGTTGGACTTGTTGTTGCATTTTTGTATAA
TAAATACAAAAATATTAAACTACCACAAACACTTTCTTTTTTTGGAGGCCGCAGATTTGTGCCTATGTTAGGAATTCTTT
CAATAATTCCGCTTGGTTTGATATTTGCAATTATTTGACCTTGAGCCCAATATGGACTAATTAAAATTGGTTCAGCTCTA
TCAGATCAAAGTGCAAATCATTATGCTCGAGGGTTTTATGTTTCAATTTATGCATTTTTAAATAGATTATTACAACCTTT
TGGACTTCATCATATTTTGAATACTTTTGTCTGATTTCAATTGCCAATTAAGGGTAAATTAATTTCCCCCGTGATTAATC
AGGTGGGAGAAACTGTTGCAAAATTAGGTGAAATTTATACTGTAAATGGGGATATAACTGCATTTAATACTGGAATTATT
GGCTCTGGTGGGTTTACAACCGGATTTTTCCCGTTATATTTAGGGGGTCTTCCTGGGGCTGCAGTTGCAATGATTTTTGC
TGCAAAAAAAGAAAGAAGAAAAACAATTACTACCTTTTTAGCAGGGGCAACGCTTGTAAGTTTTCTTACTGGAATTGATG
AGCCTTTAATTTTTACTTTTATTTTCATCTCACCACTTTTATGACTTTTAAATGCTTTTTTGACCTCAGTAATTTACATG
TTTGTATCTTGAACTGGAATGAGTATCGGAATTGGATTTTCAGCTGGATTTATTGATTATATTGTTTCTTTTCCAAGGTC
ATGGGCGTTTGCAAAAAACTTTGGAGTTTTGGCAAATCCACTATGAATTTGGGCTTTTTCCGCGATAATGTTTGTAATTC
AAGGTTCAAGTTTTTTCTTTTGTATTAAAAAGTTTAATATTAAAACTTTGGGTCGTGAAGATAAAATTGAGCCAGAATTT
AGTATAATAGATGAAAAAACTGACTTGGAAAATCAGGAAACATTTCTACCTGATCAAGAAAAAATAACTAAAAATAAAGT
AAAATTTGCTGATGACTATCAAAAAATGGCAGCAGATTTTATTGAAATTTTAGGTAAGGAAAATATTGAAGAAGTCTCAA
ATTGTGCAACACGGCTAAGATTAATTCTTAAAGATAATGCAAAAAATGATGAATTAGATGCAAAAATTACAGCCGCCGGC
GGCCGTGGAGTTGTCCGTGTTGGAAATAAAGGATATCAAATTATTATTGGAACCGATGTTGAATTTGTAGCTGATTATCT
TAAAAAAATGATAGGAAAATAA

Upstream 100 bases:

>100_bases
ATTGCACTTTCATTTTAGATAAAAAAGCAGCATCAAAATTATCAGAAAAAACTTTAAAAAGTGCAATTTGGTATTAAAAA
ATTTAGAAAAACTAGTTATA

Downstream 100 bases:

>100_bases
TGGTTTTTATCGATCCAAAAGAAACATTTTCAAAACTAAATGCAGAGAATAAACCAATTTTTGCTTTTAATGTTATAAAT
TTAGAAACACTTTTTGCTGT

Product: pts system, n-acetylglucosamine-specific enzyme II, ABC component

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MSVGFVKKIKFFRPKKAENYSQNSIFNRILNQLQRLGRSLMFPIAVLPIAALLLRIGALIQDPLANGSIGISETQKFWGR
LISTPGGIVFDNLAIIFAIGIGFGFAKDNRGEAALVAAVVWYGMSTLLKQNQLAEAIYKNVLVAKSGQLKGFTQLLYFLK
AGKPVYQLDSGVIGGISVGLVVAFLYNKYKNIKLPQTLSFFGGRRFVPMLGILSIIPLGLIFAIIWPWAQYGLIKIGSAL
SDQSANHYARGFYVSIYAFLNRLLQPFGLHHILNTFVWFQLPIKGKLISPVINQVGETVAKLGEIYTVNGDITAFNTGII
GSGGFTTGFFPLYLGGLPGAAVAMIFAAKKERRKTITTFLAGATLVSFLTGIDEPLIFTFIFISPLLWLLNAFLTSVIYM
FVSWTGMSIGIGFSAGFIDYIVSFPRSWAFAKNFGVLANPLWIWAFSAIMFVIQGSSFFFCIKKFNIKTLGREDKIEPEF
SIIDEKTDLENQETFLPDQEKITKNKVKFADDYQKMAADFIEILGKENIEEVSNCATRLRLILKDNAKNDELDAKITAAG
GRGVVRVGNKGYQIIIGTDVEFVADYLKKMIGK

Sequences:

>Translated_593_residues
MSVGFVKKIKFFRPKKAENYSQNSIFNRILNQLQRLGRSLMFPIAVLPIAALLLRIGALIQDPLANGSIGISETQKFWGR
LISTPGGIVFDNLAIIFAIGIGFGFAKDNRGEAALVAAVV*YGMSTLLKQNQLAEAIYKNVLVAKSGQLKGFTQLLYFLK
AGKPVYQLDSGVIGGISVGLVVAFLYNKYKNIKLPQTLSFFGGRRFVPMLGILSIIPLGLIFAII*P*AQYGLIKIGSAL
SDQSANHYARGFYVSIYAFLNRLLQPFGLHHILNTFV*FQLPIKGKLISPVINQVGETVAKLGEIYTVNGDITAFNTGII
GSGGFTTGFFPLYLGGLPGAAVAMIFAAKKERRKTITTFLAGATLVSFLTGIDEPLIFTFIFISPLL*LLNAFLTSVIYM
FVS*TGMSIGIGFSAGFIDYIVSFPRSWAFAKNFGVLANPL*IWAFSAIMFVIQGSSFFFCIKKFNIKTLGREDKIEPEF
SIIDEKTDLENQETFLPDQEKITKNKVKFADDYQKMAADFIEILGKENIEEVSNCATRLRLILKDNAKNDELDAKITAAG
GRGVVRVGNKGYQIIIGTDVEFVADYLKKMIGK
>Mature_592_residues
SVGFVKKIKFFRPKKAENYSQNSIFNRILNQLQRLGRSLMFPIAVLPIAALLLRIGALIQDPLANGSIGISETQKFWGRL
ISTPGGIVFDNLAIIFAIGIGFGFAKDNRGEAALVAAVV*YGMSTLLKQNQLAEAIYKNVLVAKSGQLKGFTQLLYFLKA
GKPVYQLDSGVIGGISVGLVVAFLYNKYKNIKLPQTLSFFGGRRFVPMLGILSIIPLGLIFAII*P*AQYGLIKIGSALS
DQSANHYARGFYVSIYAFLNRLLQPFGLHHILNTFV*FQLPIKGKLISPVINQVGETVAKLGEIYTVNGDITAFNTGIIG
SGGFTTGFFPLYLGGLPGAAVAMIFAAKKERRKTITTFLAGATLVSFLTGIDEPLIFTFIFISPLL*LLNAFLTSVIYMF
VS*TGMSIGIGFSAGFIDYIVSFPRSWAFAKNFGVLANPL*IWAFSAIMFVIQGSSFFFCIKKFNIKTLGREDKIEPEFS
IIDEKTDLENQETFLPDQEKITKNKVKFADDYQKMAADFIEILGKENIEEVSNCATRLRLILKDNAKNDELDAKITAAGG
RGVVRVGNKGYQIIIGTDVEFVADYLKKMIGK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=565, Percent_Identity=35.5752212389381, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1787343, Length=561, Percent_Identity=31.9073083778966, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1787908, Length=572, Percent_Identity=27.7972027972028, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 64171; Mature: 64040

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCEEEEECCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVGFVKKIKFFRPKKAENYSQNSIFNRILNQLQRLGRSLMFPIAVLPIAALLLRIGALI
CCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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CCCCCCEEEEECCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]