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The map label for this gene is potB

Identifier: 54020582

GI number: 54020582

Start: 705387

End: 706247

Strand: Reverse

Name: potB

Synonym: mhp558

Alternate gene names: 54020582

Gene position: 706247-705387 (Counterclockwise)

Preceding gene: 54020583

Following gene: 54020581

Centisome position: 79.11

GC content: 28.11

Gene sequence:

>861_bases
ATGCTGAAATTTAATTTTTTTGAAAATTTTTGAAAAAATTTCAAAAAAAGTTTAAACCCCCGCATCAGTCTTGTAATTCC
CTATGTAATTTTTGCCCTGATTTTTGTAATAATTCCATTAATTTTACTTTTTGGAAAATCAGTTAGTCCGCTTGTACATA
ATAGTCAAAGTTTTGATAATCATTTATTAATAAAGGAAAAAACTACCTGGCAAATTATTGGCCGTTCAATTTTTGTTGGT
CTAATTTCGGCTTTTATTTGTTTAATTTTAGCATTTCCCTATGCTTTTTTTGTTGCAAATTCAAGATCAAAAATATTCAA
AATTTATTCGCTTTCATTGATAGCTAGTCCCTTAATTATTTTCACAATTGCTAAAGTTTTTGCGCTTAGAAGTTTATTTT
TACTACTTTTTGATGAAGGTAGTCTTAATAATAATTCTTTTATGGTCTTAGGTTTAATTTTTTTGAATTTTCCATTTATG
GTACTGCCATTATATACAATTTTTCGCGATATGCCCAAAAATTTACTAGAAGCCGGGACTGATTTAGGTTATTCAAAATT
TTGACTTTTAATAAAAGTTGTTCTTCCTTATAGTTTTCGGGCAATTAGTTCCGGTTTTGCGCTTGTTTTTTTAATGGCAG
CTACCTCAATTGTGGTTTCAGATAAACTTTTACCAAATGGATCACAAAACCAATTAATTGGCAATTTGATTAATAATTCT
GCAAATATTTCCAATCCTTTTGATCTTGCCCGGGTTTCATCGCTTGTTTTGGTGACCCTGCTAGTTTTTATTGGAATTTA
TAGTCTAATTCATTTTATTCCTATTATTATAATGAAAATTAAAGGATTTAAATATGACTAA

Upstream 100 bases:

>100_bases
AATACAATAATTAATGTCGAAACTAACGATTTTTATGACCTCGAGACTCAAGTTTTTATAAAATGAGATGATGATGCTAT
TCATTTAATGGAGATGGAAA

Downstream 100 bases:

>100_bases
AATTATTGATTTTTTCCAAAAACATGAAATTTTGAAAAAATCATACGTTTGATTTTTAATTATTCTTTTTTATATTCCGA
TTATTGTCGGGGCAGTTTTT

Product: spermidine/putrescine ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLKFNFFENFWKNFKKSLNPRISLVIPYVIFALIFVIIPLILLFGKSVSPLVHNSQSFDNHLLIKEKTTWQIIGRSIFVG
LISAFICLILAFPYAFFVANSRSKIFKIYSLSLIASPLIIFTIAKVFALRSLFLLLFDEGSLNNNSFMVLGLIFLNFPFM
VLPLYTIFRDMPKNLLEAGTDLGYSKFWLLIKVVLPYSFRAISSGFALVFLMAATSIVVSDKLLPNGSQNQLIGNLINNS
ANISNPFDLARVSSLVLVTLLVFIGIYSLIHFIPIIIMKIKGFKYD

Sequences:

>Translated_286_residues
MLKFNFFENF*KNFKKSLNPRISLVIPYVIFALIFVIIPLILLFGKSVSPLVHNSQSFDNHLLIKEKTTWQIIGRSIFVG
LISAFICLILAFPYAFFVANSRSKIFKIYSLSLIASPLIIFTIAKVFALRSLFLLLFDEGSLNNNSFMVLGLIFLNFPFM
VLPLYTIFRDMPKNLLEAGTDLGYSKF*LLIKVVLPYSFRAISSGFALVFLMAATSIVVSDKLLPNGSQNQLIGNLINNS
ANISNPFDLARVSSLVLVTLLVFIGIYSLIHFIPIIIMKIKGFKYD
>Mature_286_residues
MLKFNFFENF*KNFKKSLNPRISLVIPYVIFALIFVIIPLILLFGKSVSPLVHNSQSFDNHLLIKEKTTWQIIGRSIFVG
LISAFICLILAFPYAFFVANSRSKIFKIYSLSLIASPLIIFTIAKVFALRSLFLLLFDEGSLNNNSFMVLGLIFLNFPFM
VLPLYTIFRDMPKNLLEAGTDLGYSKF*LLIKVVLPYSFRAISSGFALVFLMAATSIVVSDKLLPNGSQNQLIGNLINNS
ANISNPFDLARVSSLVLVTLLVFIGIYSLIHFIPIIIMKIKGFKYD

Specific function: Required for the activity of the bacterial transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=275, Percent_Identity=28.3636363636364, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787080, Length=207, Percent_Identity=28.5024154589372, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31969; Mature: 31969

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFNFFENFKNFKKSLNPRISLVIPYVIFALIFVIIPLILLFGKSVSPLVHNSQSFDNH
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCE
LLIKEKTTWQIIGRSIFVGLISAFICLILAFPYAFFVANSRSKIFKIYSLSLIASPLIIF
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TIAKVFALRSLFLLLFDEGSLNNNSFMVLGLIFLNFPFMVLPLYTIFRDMPKNLLEAGTD
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
LGYSKFLLIKVVLPYSFRAISSGFALVFLMAATSIVVSDKLLPNGSQNQLIGNLINNSAN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
ISNPFDLARVSSLVLVTLLVFIGIYSLIHFIPIIIMKIKGFKYD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKFNFFENFKNFKKSLNPRISLVIPYVIFALIFVIIPLILLFGKSVSPLVHNSQSFDNH
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCE
LLIKEKTTWQIIGRSIFVGLISAFICLILAFPYAFFVANSRSKIFKIYSLSLIASPLIIF
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TIAKVFALRSLFLLLFDEGSLNNNSFMVLGLIFLNFPFMVLPLYTIFRDMPKNLLEAGTD
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
LGYSKFLLIKVVLPYSFRAISSGFALVFLMAATSIVVSDKLLPNGSQNQLIGNLINNSAN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
ISNPFDLARVSSLVLVTLLVFIGIYSLIHFIPIIIMKIKGFKYD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]