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The map label for this gene is nfo

Identifier: 54020015

GI number: 54020015

Start: 76970

End: 77800

Strand: Direct

Name: nfo

Synonym: mhp065

Alternate gene names: 54020015

Gene position: 76970-77800 (Clockwise)

Preceding gene: 54020014

Following gene: 54020016

Centisome position: 8.62

GC content: 30.93

Gene sequence:

>831_bases
ATGATAAAAATAGGCTCCCATGTAAGATTCAGAAAACCTGATTATCTTTTTGGTGCAATTCAAGAATCACTAGAAAATAA
AGCAAATGCTGCAATGATTTTTCTAGGACCTCCACAGTCAACTTTCCGAGTCAAGCCCGAGAATTATAAACTCCAAGATT
ATCAAAAACACTTTTTTAAACAAATTCCGCCTGAAGATATTATTGTTCATGCCCCTTATATTATAAATCCGGCTAGTCCT
ATAAAAGCACAATTTTCTAATGATTTTTTAGTAAAGGAGATCGAAAAAATAAACTATATCGGGGCAAAATTTTTGGTTCT
ACACCCTGGATTTTTCACAAGTTTTACTAAAGAAGTGGCAAAAAAACAGTTAATTTCATCACTAAAATCAATTTTAGAAA
AAACTAAAAATGTAATTTTATTACTTGAGACAATGTCTGGAAAAGGTAGTGAAATGTGCGCTAATTTTGAAGAAATTGTT
GAAATAGTTGAAGCAGTTGAATCACCAAGAATCGGAATCTGTCTTGATACATGCCATGTCTGGGATGCTGGATATGATCT
TAAAAATTTTCCTGAATTTTGTAAAGAAATAAGAAGAACAAGGCTAATTAATTATTTGAAAGTGATCCATTTAAATGATT
CTTTAAGCCCACTAGGTTCAAAAAAGGATCGACATGCTAATATTGGCAAAGGTTTTATTGGGCTTGAAAGCTTACGCAAA
ATTATTTTTGACCCACTTTTTGCTAATATTCCTAAAATTTTAGAGACACCTTATGTTGATAATAAGCCTATTTATGATCA
GGAAATTGCGCTTTTATTAAAAAAAGTTTAA

Upstream 100 bases:

>100_bases
TTTTTGTCTTTGAAATTGAGCGAATTTTAAAGGAAAAATATAGCAAAATTCCAACCTTTACAGTTAAATTAGCTGAAATT
TTAAAAGAAATGAGATTATT

Downstream 100 bases:

>100_bases
AATTTAAAGGTTTTTTATGTCATATATAGAAAAGCAAGAATTTCTCACTGAATTAAAAACAAGAAATATTCTAAAGGATA
TCAGCAGTCCTGAAAAATTT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MIKIGSHVRFRKPDYLFGAIQESLENKANAAMIFLGPPQSTFRVKPENYKLQDYQKHFFKQIPPEDIIVHAPYIINPASP
IKAQFSNDFLVKEIEKINYIGAKFLVLHPGFFTSFTKEVAKKQLISSLKSILEKTKNVILLLETMSGKGSEMCANFEEIV
EIVEAVESPRIGICLDTCHVWDAGYDLKNFPEFCKEIRRTRLINYLKVIHLNDSLSPLGSKKDRHANIGKGFIGLESLRK
IIFDPLFANIPKILETPYVDNKPIYDQEIALLLKKV

Sequences:

>Translated_276_residues
MIKIGSHVRFRKPDYLFGAIQESLENKANAAMIFLGPPQSTFRVKPENYKLQDYQKHFFKQIPPEDIIVHAPYIINPASP
IKAQFSNDFLVKEIEKINYIGAKFLVLHPGFFTSFTKEVAKKQLISSLKSILEKTKNVILLLETMSGKGSEMCANFEEIV
EIVEAVESPRIGICLDTCHVWDAGYDLKNFPEFCKEIRRTRLINYLKVIHLNDSLSPLGSKKDRHANIGKGFIGLESLRK
IIFDPLFANIPKILETPYVDNKPIYDQEIALLLKKV
>Mature_276_residues
MIKIGSHVRFRKPDYLFGAIQESLENKANAAMIFLGPPQSTFRVKPENYKLQDYQKHFFKQIPPEDIIVHAPYIINPASP
IKAQFSNDFLVKEIEKINYIGAKFLVLHPGFFTSFTKEVAKKQLISSLKSILEKTKNVILLLETMSGKGSEMCANFEEIV
EIVEAVESPRIGICLDTCHVWDAGYDLKNFPEFCKEIRRTRLINYLKVIHLNDSLSPLGSKKDRHANIGKGFIGLESLRK
IIFDPLFANIPKILETPYVDNKPIYDQEIALLLKKV

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=286, Percent_Identity=32.5174825174825, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531193, Length=232, Percent_Identity=34.9137931034483, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322735, Length=286, Percent_Identity=31.8181818181818, Blast_Score=142, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_MYCH2 (Q601Y6)

Other databases:

- EMBL:   AE017332
- RefSeq:   YP_115579.1
- ProteinModelPortal:   Q601Y6
- SMR:   Q601Y6
- STRING:   Q601Y6
- GeneID:   3105687
- GenomeReviews:   AE017332_GR
- KEGG:   mhy:mhp065
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- PhylomeDB:   Q601Y6
- ProtClustDB:   PRK01060
- BioCyc:   MHYO295358:MHP065-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31540; Mature: 31540

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIGSHVRFRKPDYLFGAIQESLENKANAAMIFLGPPQSTFRVKPENYKLQDYQKHFFK
CEECCCCCEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHH
QIPPEDIIVHAPYIINPASPIKAQFSNDFLVKEIEKINYIGAKFLVLHPGFFTSFTKEVA
HCCCCCEEEECCEEECCCCCCHHHCCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHH
KKQLISSLKSILEKTKNVILLLETMSGKGSEMCANFEEIVEIVEAVESPRIGICLDTCHV
HHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCHHHHHHHHHHHCCCCCEEEEEEHHH
WDAGYDLKNFPEFCKEIRRTRLINYLKVIHLNDSLSPLGSKKDRHANIGKGFIGLESLRK
CCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
IIFDPLFANIPKILETPYVDNKPIYDQEIALLLKKV
HHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MIKIGSHVRFRKPDYLFGAIQESLENKANAAMIFLGPPQSTFRVKPENYKLQDYQKHFFK
CEECCCCCEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHH
QIPPEDIIVHAPYIINPASPIKAQFSNDFLVKEIEKINYIGAKFLVLHPGFFTSFTKEVA
HCCCCCEEEECCEEECCCCCCHHHCCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHH
KKQLISSLKSILEKTKNVILLLETMSGKGSEMCANFEEIVEIVEAVESPRIGICLDTCHV
HHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCHHHHHHHHHHHCCCCCEEEEEEHHH
WDAGYDLKNFPEFCKEIRRTRLINYLKVIHLNDSLSPLGSKKDRHANIGKGFIGLESLRK
CCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
IIFDPLFANIPKILETPYVDNKPIYDQEIALLLKKV
HHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA