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The map label for this gene is oppA

Identifier: 53723157

GI number: 53723157

Start: 2898490

End: 2900154

Strand: Reverse

Name: oppA

Synonym: BPSS2141

Alternate gene names: 53723157

Gene position: 2900154-2898490 (Counterclockwise)

Preceding gene: 53723158

Following gene: 53723156

Centisome position: 91.4

GC content: 64.92

Gene sequence:

>1665_bases
TTGCGCCGCGCGTTGCCATTCCGCTACCACTATCAATCGCACACGATGAAACACACGCATGCCTTCGCGGCCGTGCTGGC
CGCGCTCGCCCTGACGATCGCGCCATCCGCCCCGGCCGTCACCGTTGCCTCGAATGTCACGCTCGCGGATCAGCAGGACC
TCACGCGGCAGGTGCCCGCGGAGGTCGAATCGCTCGACCCCGCGCACATCGAATCGTGGACCGGCAACACGATCGGCCTG
GATCTGTTCGAGGGGCTCGCGCGCATCGACGCGAGCGGGGCGGTGGTGCCGGGCGTCGCGCAGGCGTGGGAGCACAAGGC
GCCGGATACGTGGATCTTCAAGCTGCGCCGCGACGCGAAGTGGAGCAACGGGCAGCCCGTGACGGCCGCGGATTTCGTAT
ACGCGTGGCAGCGTCTCGCCGATCCGAAGACGGGTTCGAAATACACGATCCTCGTCGAGTTCGTGAAGAACGCCTCGGCG
ATCATCGCGGGCAAGCAGCCGCCCGGCGATCTCGGCATTCGCGCGATCGATCCGTACACGATCGAGGTGAAGACCGAGGT
GCCCGTGTCGTATTTCCCCGAGCTCACCGCGATGGCGCCGCTCACCCCCGTGAACAAGGACGCGGTCGCGAAGTTCGGCG
ACGCATGGACGCGCCCGAAGAACATCGTGAGCAACGGCCCTTACACGCTCGTCGACTGGCAGCCGAACAACCGGATCGTG
ATGGCGAAGAGCGACAAGTACTGGAACGCGCGCAACGTCGTGATCCGCAAGGTGACGTACCTGCCGATCGAGAACGACGA
GACCGCGCTGCGGATGTACCAGGCGGGCCAGATCGACTACACGTATTCGATTCCCGCGGGCGGCTTCGGCCAGATCAGCA
AGCAGTTCGGCAAGGAGCTGCGCCCGGGGCTGCAGCTCGCGACGTATTACTACTACCTGAAGAACAGCGATCCGGCGCTC
AAGGACAAGCGCGTGCGCGAAGCGCTCGCGATGGTGCTCGACCGCGAGATCCTCACGTCGAAGATCACGCAGGCGGGCGA
AGTGCCGATGTACGGGCTGATGCCGAAGGGCGTGAAGGGCGTGCAGCGGCCGTTCACGCCGGACTGGGCGTCGTGGCCGA
TGGCGAGGCGCGTCGACTACGCGAAGAACCTGCTGAAGCAGGCGGGCCATGGCGACGCGAATCCGCTCACGTTCACGCTG
ACCTACAACACGAACGACCTGCACAAGAAGGTCGCGCTGTTCGCGGCGTCCGAATGGCGCACGAAGCTCGGCGTGACGGC
GAAGCTCGAGAACGTCGAGTTCAAGGTGCTGATGAAGCAGCGCCACGACGGCAAGGTGCAGATCGCGCGCGACGGCTGGT
TCGCCGACTACAACGACGCGATGACGTTCTTCGACCTGATCCGCTGCGGCAGCTCGCAGAACACGGTCGGCTACTGCAAC
CCGAAGGTCGATTCGCTCGTCGCCGAGGCGAACCAGAAGCTCGATGACGGCGCGCGCGCGGCGCTGCTCACGCAGGCGCA
CGATCTCGCGATGAACGACTATCCGATGGTGCCGCTCTTCCAGTATTCGGCGGACCGCCTCGTGAAGTCGTACGTCGGCG
GCTACACGCTGACGAACTACATCGACATGCGCGCCTCGCAGGACATGTACCTGATCAAGCACTGA

Upstream 100 bases:

>100_bases
TGTCGAAAGGAACGTAAGGATGACTAACATGAAATACAGGCACCAGGGGCCCCCCGTTTCTCCGCAGCCCTAAGCGTTCT
TCGCGCCGTCGCGCACGGCC

Downstream 100 bases:

>100_bases
CTGGAGCGGAGCCGATCATGCTGGCCTACGCATTGCGGCGCACGCTATGGGCGGTGCCGACGATCCTCGCCGTCGTCACG
GTCTGCTATCTGCTGCTGCA

Product: periplasmic oligopeptide-binding protein precursor

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MRRALPFRYHYQSHTMKHTHAFAAVLAALALTIAPSAPAVTVASNVTLADQQDLTRQVPAEVESLDPAHIESWTGNTIGL
DLFEGLARIDASGAVVPGVAQAWEHKAPDTWIFKLRRDAKWSNGQPVTAADFVYAWQRLADPKTGSKYTILVEFVKNASA
IIAGKQPPGDLGIRAIDPYTIEVKTEVPVSYFPELTAMAPLTPVNKDAVAKFGDAWTRPKNIVSNGPYTLVDWQPNNRIV
MAKSDKYWNARNVVIRKVTYLPIENDETALRMYQAGQIDYTYSIPAGGFGQISKQFGKELRPGLQLATYYYYLKNSDPAL
KDKRVREALAMVLDREILTSKITQAGEVPMYGLMPKGVKGVQRPFTPDWASWPMARRVDYAKNLLKQAGHGDANPLTFTL
TYNTNDLHKKVALFAASEWRTKLGVTAKLENVEFKVLMKQRHDGKVQIARDGWFADYNDAMTFFDLIRCGSSQNTVGYCN
PKVDSLVAEANQKLDDGARAALLTQAHDLAMNDYPMVPLFQYSADRLVKSYVGGYTLTNYIDMRASQDMYLIKH

Sequences:

>Translated_554_residues
MRRALPFRYHYQSHTMKHTHAFAAVLAALALTIAPSAPAVTVASNVTLADQQDLTRQVPAEVESLDPAHIESWTGNTIGL
DLFEGLARIDASGAVVPGVAQAWEHKAPDTWIFKLRRDAKWSNGQPVTAADFVYAWQRLADPKTGSKYTILVEFVKNASA
IIAGKQPPGDLGIRAIDPYTIEVKTEVPVSYFPELTAMAPLTPVNKDAVAKFGDAWTRPKNIVSNGPYTLVDWQPNNRIV
MAKSDKYWNARNVVIRKVTYLPIENDETALRMYQAGQIDYTYSIPAGGFGQISKQFGKELRPGLQLATYYYYLKNSDPAL
KDKRVREALAMVLDREILTSKITQAGEVPMYGLMPKGVKGVQRPFTPDWASWPMARRVDYAKNLLKQAGHGDANPLTFTL
TYNTNDLHKKVALFAASEWRTKLGVTAKLENVEFKVLMKQRHDGKVQIARDGWFADYNDAMTFFDLIRCGSSQNTVGYCN
PKVDSLVAEANQKLDDGARAALLTQAHDLAMNDYPMVPLFQYSADRLVKSYVGGYTLTNYIDMRASQDMYLIKH
>Mature_554_residues
MRRALPFRYHYQSHTMKHTHAFAAVLAALALTIAPSAPAVTVASNVTLADQQDLTRQVPAEVESLDPAHIESWTGNTIGL
DLFEGLARIDASGAVVPGVAQAWEHKAPDTWIFKLRRDAKWSNGQPVTAADFVYAWQRLADPKTGSKYTILVEFVKNASA
IIAGKQPPGDLGIRAIDPYTIEVKTEVPVSYFPELTAMAPLTPVNKDAVAKFGDAWTRPKNIVSNGPYTLVDWQPNNRIV
MAKSDKYWNARNVVIRKVTYLPIENDETALRMYQAGQIDYTYSIPAGGFGQISKQFGKELRPGLQLATYYYYLKNSDPAL
KDKRVREALAMVLDREILTSKITQAGEVPMYGLMPKGVKGVQRPFTPDWASWPMARRVDYAKNLLKQAGHGDANPLTFTL
TYNTNDLHKKVALFAASEWRTKLGVTAKLENVEFKVLMKQRHDGKVQIARDGWFADYNDAMTFFDLIRCGSSQNTVGYCN
PKVDSLVAEANQKLDDGARAALLTQAHDLAMNDYPMVPLFQYSADRLVKSYVGGYTLTNYIDMRASQDMYLIKH

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=545, Percent_Identity=42.7522935779817, Blast_Score=437, Evalue=1e-123,
Organism=Escherichia coli, GI87081878, Length=547, Percent_Identity=36.745886654479, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1789397, Length=529, Percent_Identity=37.4291115311909, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1787052, Length=530, Percent_Identity=23.0188679245283, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787762, Length=541, Percent_Identity=23.2902033271719, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789966, Length=555, Percent_Identity=22.7027027027027, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789887, Length=492, Percent_Identity=23.1707317073171, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61885; Mature: 61885

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRALPFRYHYQSHTMKHTHAFAAVLAALALTIAPSAPAVTVASNVTLADQQDLTRQVPA
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEECCHHHHHHHCCH
EVESLDPAHIESWTGNTIGLDLFEGLARIDASGAVVPGVAQAWEHKAPDTWIFKLRRDAK
HHHCCCCHHHHCCCCCEECHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCC
WSNGQPVTAADFVYAWQRLADPKTGSKYTILVEFVKNASAIIAGKQPPGDLGIRAIDPYT
CCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHCCCEEEECCCCCCCCCEEEECCEE
IEVKTEVPVSYFPELTAMAPLTPVNKDAVAKFGDAWTRPKNIVSNGPYTLVDWQPNNRIV
EEEEECCCHHHCCCHHHCCCCCCCCHHHHHHHCCCCCCCCHHHCCCCEEEEEECCCCEEE
MAKSDKYWNARNVVIRKVTYLPIENDETALRMYQAGQIDYTYSIPAGGFGQISKQFGKEL
EEECCCCCCCCCEEEEEEEEEECCCCCHHEEEEECCCEEEEEECCCCCHHHHHHHHHHHH
RPGLQLATYYYYLKNSDPALKDKRVREALAMVLDREILTSKITQAGEVPMYGLMPKGVKG
CCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHH
VQRPFTPDWASWPMARRVDYAKNLLKQAGHGDANPLTFTLTYNTNDLHKKVALFAASEWR
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHEEEHHHHH
TKLGVTAKLENVEFKVLMKQRHDGKVQIARDGWFADYNDAMTFFDLIRCGSSQNTVGYCN
HHCCCEEEECCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECC
PKVDSLVAEANQKLDDGARAALLTQAHDLAMNDYPMVPLFQYSADRLVKSYVGGYTLTNY
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCEEEEHE
IDMRASQDMYLIKH
EECCCCCCEEEEEC
>Mature Secondary Structure
MRRALPFRYHYQSHTMKHTHAFAAVLAALALTIAPSAPAVTVASNVTLADQQDLTRQVPA
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEECCHHHHHHHCCH
EVESLDPAHIESWTGNTIGLDLFEGLARIDASGAVVPGVAQAWEHKAPDTWIFKLRRDAK
HHHCCCCHHHHCCCCCEECHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCC
WSNGQPVTAADFVYAWQRLADPKTGSKYTILVEFVKNASAIIAGKQPPGDLGIRAIDPYT
CCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHCCCEEEECCCCCCCCCEEEECCEE
IEVKTEVPVSYFPELTAMAPLTPVNKDAVAKFGDAWTRPKNIVSNGPYTLVDWQPNNRIV
EEEEECCCHHHCCCHHHCCCCCCCCHHHHHHHCCCCCCCCHHHCCCCEEEEEECCCCEEE
MAKSDKYWNARNVVIRKVTYLPIENDETALRMYQAGQIDYTYSIPAGGFGQISKQFGKEL
EEECCCCCCCCCEEEEEEEEEECCCCCHHEEEEECCCEEEEEECCCCCHHHHHHHHHHHH
RPGLQLATYYYYLKNSDPALKDKRVREALAMVLDREILTSKITQAGEVPMYGLMPKGVKG
CCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHH
VQRPFTPDWASWPMARRVDYAKNLLKQAGHGDANPLTFTLTYNTNDLHKKVALFAASEWR
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHEEEHHHHH
TKLGVTAKLENVEFKVLMKQRHDGKVQIARDGWFADYNDAMTFFDLIRCGSSQNTVGYCN
HHCCCEEEECCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECC
PKVDSLVAEANQKLDDGARAALLTQAHDLAMNDYPMVPLFQYSADRLVKSYVGGYTLTNY
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCEEEEHE
IDMRASQDMYLIKH
EECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187863; 9097039; 9278503; 2015910; 9298646; 9600841 [H]