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The map label for this gene is puuR [H]

Identifier: 53723158

GI number: 53723158

Start: 2900290

End: 2900871

Strand: Reverse

Name: puuR [H]

Synonym: BPSS2142

Alternate gene names: 53723158

Gene position: 2900871-2900290 (Counterclockwise)

Preceding gene: 53723161

Following gene: 53723157

Centisome position: 91.42

GC content: 63.57

Gene sequence:

>582_bases
ATGGTTTCTTCCACTGAAAACACGCGGCTTGCCAATGCTGGCGTGGCGCTCGGGAGCAAGATTCGGGCGTTGCGGCAGCG
GCTGAAGCGCACGCTCGACGAAACAGCGACTGCCGCGGGTATTTCCAAGCCGTTTTTGTCGCAGGTCGAACGCGGGCTCG
CATCGCCGTCCATCACGTCGCTGGCCGGTATCGCGCACGCGCTGGGCGTGACGGTGCAGTACTTCGTCGAGACGCCGAGC
GAAGAGCGTTCGGTCTGCCGCGGCGACCAGTTGCGCTTTTTCAGCTTCGCCGATTCGGCGAACCTGTTCGCGCGGCTGAC
GAACGTGCCGGAGGGGCGTCAGCTCGAGGCGATCCTCGTGCGGATGCCGCCGGGGCAGAAGCGCTCTGAAGTGACGACGC
ATGCAGGCGAGGAATTCCTGTATGTGATCGAGGGTGAAGTGTCGTTGACGCTGGAAGGCAAGTCGTTCGTGCTGCATGCC
GGCGACAGCGCGCATTACCAGTCGACGGTCCCGCACAGCTGGGTCAACACCGCGAAGATCGAGTCGGTGGTGGTCTGGGT
GGGAACGCCGAGGTTGTTCTAG

Upstream 100 bases:

>100_bases
GAGGCGGCGGCGCAGCTCGGGACGACGAAGAGTAAGTCATATCTATCAAACTTCGTTATTTTCCATCAAACATTTTTTGA
CGATAAGATTTCGGCCTGCT

Downstream 100 bases:

>100_bases
GGCGGGGCATTCCTTCGTGTGTTCGATGGGGTGCCTGTCGAAAGGAACGTAAGGATGACTAACATGAAATACAGGCACCA
GGGGCCCCCCGTTTCTCCGC

Product: MerR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MVSSTENTRLANAGVALGSKIRALRQRLKRTLDETATAAGISKPFLSQVERGLASPSITSLAGIAHALGVTVQYFVETPS
EERSVCRGDQLRFFSFADSANLFARLTNVPEGRQLEAILVRMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKSFVLHA
GDSAHYQSTVPHSWVNTAKIESVVVWVGTPRLF

Sequences:

>Translated_193_residues
MVSSTENTRLANAGVALGSKIRALRQRLKRTLDETATAAGISKPFLSQVERGLASPSITSLAGIAHALGVTVQYFVETPS
EERSVCRGDQLRFFSFADSANLFARLTNVPEGRQLEAILVRMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKSFVLHA
GDSAHYQSTVPHSWVNTAKIESVVVWVGTPRLF
>Mature_193_residues
MVSSTENTRLANAGVALGSKIRALRQRLKRTLDETATAAGISKPFLSQVERGLASPSITSLAGIAHALGVTVQYFVETPS
EERSVCRGDQLRFFSFADSANLFARLTNVPEGRQLEAILVRMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKSFVLHA
GDSAHYQSTVPHSWVNTAKIESVVVWVGTPRLF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=187, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20999; Mature: 20999

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSTENTRLANAGVALGSKIRALRQRLKRTLDETATAAGISKPFLSQVERGLASPSITS
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
LAGIAHALGVTVQYFVETPSEERSVCRGDQLRFFSFADSANLFARLTNVPEGRQLEAILV
HHHHHHHHHHEEEEEEECCCHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEEE
RMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKSFVLHAGDSAHYQSTVPHSWVNTAKI
ECCCCCCCCHHHHCCCCEEEEEEECEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHEE
ESVVVWVGTPRLF
EEEEEEECCCCCC
>Mature Secondary Structure
MVSSTENTRLANAGVALGSKIRALRQRLKRTLDETATAAGISKPFLSQVERGLASPSITS
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
LAGIAHALGVTVQYFVETPSEERSVCRGDQLRFFSFADSANLFARLTNVPEGRQLEAILV
HHHHHHHHHHEEEEEEECCCHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEEE
RMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKSFVLHAGDSAHYQSTVPHSWVNTAKI
ECCCCCCCCHHHHCCCCEEEEEEECEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHEE
ESVVVWVGTPRLF
EEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]