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The map label for this gene is ansA

Identifier: 53723077

GI number: 53723077

Start: 2793880

End: 2794935

Strand: Reverse

Name: ansA

Synonym: BPSS2060

Alternate gene names: 53723077

Gene position: 2794935-2793880 (Counterclockwise)

Preceding gene: 53723079

Following gene: 53723076

Centisome position: 88.08

GC content: 59.56

Gene sequence:

>1056_bases
ATGACGCTGACAATTGGGGGCTTCACGACTGCGGCCAACGCACAGCAAGCCAGCGCGCCCCCGCCGCAAAACCTGCCGAG
GATTCTCGTGCTGGCAACGGGTGGCACGATCGCAAGCACCTCCGATCCTCGCTCGGCAATTGGCTACAACGCCGGCGGCA
TTACCGGCGATCAGCTCCTCGCGAGCGTTCCCGGCCTCGACAAGATCGCGTCCATCAAAGCGGAGCAGATTTCGAATGTC
GGCTCACAGGACATGAACGGCAAGATCTGGTATCAGCTCGCAATCCGCATCAAACAGGCCATTGACCGGAACGAGGTCGA
CGGCATTGTCATTACGCACGGCACCGACACAATGGAAGAAACCGGCTTCTTCCTCGATCACGTTCTCTCGACCAGCAAGC
CAGTTGTTCTCGTCGGTTCCATGCGTCCGGGTGGTTCGACCAGCGCCGATGGCCCGATGAATCTCTACGAAGCGGCGGAA
GTTGCTGCCAGCCCCCAGTCCGTCAACCGTGGCGTGCTGATGGTTATGAATGACACAATTAACAGCCCGCGTTGGGCAAC
CAAAACCAACACCACGTCGGTGCAGACCTTCCAGAATCCGAACGCTGGCCCGCTTGGCTATGTCGACGCTGCCTCGATCC
GCTACCTCTCTCCTGCGCCGACCAGTCATAGGAAGGCATTGGAGCTGCCAAGCGGTGGGCTGCCCCGCGTGGACATCGTG
TACGCGCACGCGGATATGGACGCGACGCAGATTCAAGACGCTGTTCAACACGACGCGAAGGGCATTGTCCTCGCAGGTGT
CGGCGATGGAAACGCGTCGAAGGCCGCACTGGACGCGATGGAAGCAGCAGCTCGCAAAGGCATCGTCGTGGTCCGTTCCA
CGCGTGTGAGCAGCGGATTCGTCAATCGGAATGTCGAAGTGCCCGACGACAAGAGCGGCTTCGTCGCGTCCTATGACCTG
AACCCTCAAAAGTCGCGTGTTCTCGCGCAATTGCTCATCGCCAACGGTGTGACTTCACCAGACAAGGTTCAGAAAGCCTT
CCAGTCAACGTACTGA

Upstream 100 bases:

>100_bases
TCGGAGCGGGGCGCTCAGTCCCCAAATCAATGCCAGGAGATCTGCAATGCAAGAGAAACACATACAGAACGCGTTGATGC
GAGGCCTCCATGCGGCGCTT

Downstream 100 bases:

>100_bases
ACTCCATTTCGTAAGCGGGTCGCCTGCAACGGCGACTTGCCAACTTTTCTCAGGCGTCCTTCGCTGCTGCAAACGCCCCA
ATCAGGCGCCGAGCCATCAC

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTLTIGGFTTAANAQQASAPPPQNLPRILVLATGGTIASTSDPRSAIGYNAGGITGDQLLASVPGLDKIASIKAEQISNV
GSQDMNGKIWYQLAIRIKQAIDRNEVDGIVITHGTDTMEETGFFLDHVLSTSKPVVLVGSMRPGGSTSADGPMNLYEAAE
VAASPQSVNRGVLMVMNDTINSPRWATKTNTTSVQTFQNPNAGPLGYVDAASIRYLSPAPTSHRKALELPSGGLPRVDIV
YAHADMDATQIQDAVQHDAKGIVLAGVGDGNASKAALDAMEAAARKGIVVVRSTRVSSGFVNRNVEVPDDKSGFVASYDL
NPQKSRVLAQLLIANGVTSPDKVQKAFQSTY

Sequences:

>Translated_351_residues
MTLTIGGFTTAANAQQASAPPPQNLPRILVLATGGTIASTSDPRSAIGYNAGGITGDQLLASVPGLDKIASIKAEQISNV
GSQDMNGKIWYQLAIRIKQAIDRNEVDGIVITHGTDTMEETGFFLDHVLSTSKPVVLVGSMRPGGSTSADGPMNLYEAAE
VAASPQSVNRGVLMVMNDTINSPRWATKTNTTSVQTFQNPNAGPLGYVDAASIRYLSPAPTSHRKALELPSGGLPRVDIV
YAHADMDATQIQDAVQHDAKGIVLAGVGDGNASKAALDAMEAAARKGIVVVRSTRVSSGFVNRNVEVPDDKSGFVASYDL
NPQKSRVLAQLLIANGVTSPDKVQKAFQSTY
>Mature_350_residues
TLTIGGFTTAANAQQASAPPPQNLPRILVLATGGTIASTSDPRSAIGYNAGGITGDQLLASVPGLDKIASIKAEQISNVG
SQDMNGKIWYQLAIRIKQAIDRNEVDGIVITHGTDTMEETGFFLDHVLSTSKPVVLVGSMRPGGSTSADGPMNLYEAAEV
AASPQSVNRGVLMVMNDTINSPRWATKTNTTSVQTFQNPNAGPLGYVDAASIRYLSPAPTSHRKALELPSGGLPRVDIVY
AHADMDATQIQDAVQHDAKGIVLAGVGDGNASKAALDAMEAAARKGIVVVRSTRVSSGFVNRNVEVPDDKSGFVASYDLN
PQKSRVLAQLLIANGVTSPDKVQKAFQSTY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=326, Percent_Identity=47.239263803681, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788065, Length=284, Percent_Identity=32.0422535211268, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI193207372, Length=333, Percent_Identity=26.1261261261261, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558278, Length=333, Percent_Identity=26.1261261261261, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558280, Length=333, Percent_Identity=26.1261261261261, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193207374, Length=333, Percent_Identity=26.1261261261261, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323189, Length=338, Percent_Identity=39.0532544378698, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323187, Length=338, Percent_Identity=39.0532544378698, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323186, Length=338, Percent_Identity=39.0532544378698, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323184, Length=338, Percent_Identity=39.0532544378698, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6320527, Length=339, Percent_Identity=38.0530973451327, Blast_Score=189, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24645530, Length=227, Percent_Identity=27.7533039647577, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36786; Mature: 36655

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTIGGFTTAANAQQASAPPPQNLPRILVLATGGTIASTSDPRSAIGYNAGGITGDQLL
CEEEECCCEECCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCHHHCCCCCCCCCHHHHH
ASVPGLDKIASIKAEQISNVGSQDMNGKIWYQLAIRIKQAIDRNEVDGIVITHGTDTMEE
HHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEEEECCCCCHHH
TGFFLDHVLSTSKPVVLVGSMRPGGSTSADGPMNLYEAAEVAASPQSVNRGVLMVMNDTI
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCC
NSPRWATKTNTTSVQTFQNPNAGPLGYVDAASIRYLSPAPTSHRKALELPSGGLPRVDIV
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCEEEEE
YAHADMDATQIQDAVQHDAKGIVLAGVGDGNASKAALDAMEAAARKGIVVVRSTRVSSGF
EEECCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCC
VNRNVEVPDDKSGFVASYDLNPQKSRVLAQLLIANGVTSPDKVQKAFQSTY
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TLTIGGFTTAANAQQASAPPPQNLPRILVLATGGTIASTSDPRSAIGYNAGGITGDQLL
EEEECCCEECCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCHHHCCCCCCCCCHHHHH
ASVPGLDKIASIKAEQISNVGSQDMNGKIWYQLAIRIKQAIDRNEVDGIVITHGTDTMEE
HHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEEEECCCCCHHH
TGFFLDHVLSTSKPVVLVGSMRPGGSTSADGPMNLYEAAEVAASPQSVNRGVLMVMNDTI
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCC
NSPRWATKTNTTSVQTFQNPNAGPLGYVDAASIRYLSPAPTSHRKALELPSGGLPRVDIV
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCEEEEE
YAHADMDATQIQDAVQHDAKGIVLAGVGDGNASKAALDAMEAAARKGIVVVRSTRVSSGF
EEECCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCC
VNRNVEVPDDKSGFVASYDLNPQKSRVLAQLLIANGVTSPDKVQKAFQSTY
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]