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The map label for this gene is gadA [C]

Identifier: 53723039

GI number: 53723039

Start: 2731210

End: 2732691

Strand: Reverse

Name: gadA [C]

Synonym: BPSS2021

Alternate gene names: 53723039

Gene position: 2732691-2731210 (Counterclockwise)

Preceding gene: 53723042

Following gene: 53723035

Centisome position: 86.12

GC content: 66.6

Gene sequence:

>1482_bases
GTGCGTTCGATCGCGGCGCGAAGTTCGCACGCCGGCGCCGGCCTCGAACAGGAGGGCGTCATGGATCTCGAAGAAGGCGT
GAGGCAGCTCTATCCGTATGCGGCGGAATTCGGCGCGTTACACGAATTTCCCGAGCGCGGGATGCCGCGTGAGCGCTTGC
TCGAAGAATTGCGGTCGATGGCCGTGCGCGAAGATCGCAAATGGGAGAGCGGCCGCTGCTCGGGCACGATGTACTGCGGC
GACCACGAACATTATGCGTTCCTGAACGAAGCGTACGGGCTGTTCAGCCATGTGAACGCGTTGCAGCGCGACCTGTGCCC
GAGCATGAACCGGATGGAAAGCGAGATCGTCGCGATGACGGTTGCGCTATTGCACGGCGAAGCCGTGCAGCGGCATGACG
GCGCGCATCGCGCATGCGGCGCGCTGAGCCTCGGCGGCACCGAGAGCATTCTGAATGCGACGCTCGCGTATCGCGAGAAA
GCGCGCGCCGAGCGCGGCATCGAGCGGCCGCGGATGATCTGGCCGGCATCCGCGCATCCGGCGTTTCGCAAGGCCGCGCA
TCTGTTCGGCTTCGACGTGACCGTCGCGCCGATCGATCCCGTCACGATGCAGGTCGATGCCGATTTCGTGCGCGACGCGG
TGGACGCGAATACGGTGATGCTCGTGGGCTCGGCATGCAATTATCCGTACGGCACGATCGATCCGATCGGCGCGCTGTCG
GCGATCGCCGTCGAAAAGGACGTGTGGCTGCACGTCGACGGGTGCCTCGGCGGCTGGATGCTGCCGTGGGGCGAAGCGCT
GGGCTATCCGGACATTCCGGCATTCGATTTTCGTCTGCCGGGCGTCACGTCGATCTCGGCCGATACGCACAAGTTCGGCT
ACGGCCCGAAGGGCGGCTCGGTGCTCGCGTGGCGCGACGCGAGCTTTCGGCGCCATCAGTATTTTCTGATGACCGATTGG
GTGGGCGGCGTCTATGGCTCGCCGGGATTGACGGGCAGCCGCTCGGGCGGCCTGATCGCGGCGACGTGGGCGGCGCTGCG
CAGCCTCGGGCGCGAAGGCTACCTCGCGCGGGCGAAGGCGATCTTCGAGACGGCCTTCGACATGCAGGCCGCGGTGCGCG
CGATTCCGGAGCTGCGCGTGCTCGGCAAGCCGACGTTCTGCTTCGCGTTCACCTCGGACGCGTTCGACATCTATCACGTG
AACGATTTCATGCGGCAGCGCGGCTGGCGCTTCAACGGGCTTCAGCATCCGGATGCGCTGCACATGTGCGTGACGGGGCC
GCAGACGCAGCCGGGCGTCGCCGAGCGGTTCCGGCAGGACCTGGGCGAAGCGGTCGAGCATGCACGGCATCGTGCGCACG
CGCGGCCGCAGTCGAGCGGCGTTTATGGCGGCGATGCGGCGGGGCTCGATTTGCGTGACGACGCGCGCGCGCGCGCGTTC
TTTACGCAGGTGCTCGATCTGTTTACCGACTGTCCGCTCTAG

Upstream 100 bases:

>100_bases
TCATAGTGTGAAAGAAAGGAACATCCAGGGGAATTTTTAATGTGTTAATTAGGCAACCTTAATTCGATCGGCAAGGCCGG
AATGGGCCGCGCCGTCGAGC

Downstream 100 bases:

>100_bases
CGCGCGAGGCGCGAAGCCGGGCGCGCCGGGCCGGCGTCGGCGCGGGTTCGATGCGCATCGCGCCGCGCACGGCGGGACCG
CCGGGCCGGCGAGCGGGGCG

Product: decarboxylase

Products: NA

Alternate protein names: SP-lyase; SPL; Sphingosine-1-phosphate aldolase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRSIAARSSHAGAGLEQEGVMDLEEGVRQLYPYAAEFGALHEFPERGMPRERLLEELRSMAVREDRKWESGRCSGTMYCG
DHEHYAFLNEAYGLFSHVNALQRDLCPSMNRMESEIVAMTVALLHGEAVQRHDGAHRACGALSLGGTESILNATLAYREK
ARAERGIERPRMIWPASAHPAFRKAAHLFGFDVTVAPIDPVTMQVDADFVRDAVDANTVMLVGSACNYPYGTIDPIGALS
AIAVEKDVWLHVDGCLGGWMLPWGEALGYPDIPAFDFRLPGVTSISADTHKFGYGPKGGSVLAWRDASFRRHQYFLMTDW
VGGVYGSPGLTGSRSGGLIAATWAALRSLGREGYLARAKAIFETAFDMQAAVRAIPELRVLGKPTFCFAFTSDAFDIYHV
NDFMRQRGWRFNGLQHPDALHMCVTGPQTQPGVAERFRQDLGEAVEHARHRAHARPQSSGVYGGDAAGLDLRDDARARAF
FTQVLDLFTDCPL

Sequences:

>Translated_493_residues
MRSIAARSSHAGAGLEQEGVMDLEEGVRQLYPYAAEFGALHEFPERGMPRERLLEELRSMAVREDRKWESGRCSGTMYCG
DHEHYAFLNEAYGLFSHVNALQRDLCPSMNRMESEIVAMTVALLHGEAVQRHDGAHRACGALSLGGTESILNATLAYREK
ARAERGIERPRMIWPASAHPAFRKAAHLFGFDVTVAPIDPVTMQVDADFVRDAVDANTVMLVGSACNYPYGTIDPIGALS
AIAVEKDVWLHVDGCLGGWMLPWGEALGYPDIPAFDFRLPGVTSISADTHKFGYGPKGGSVLAWRDASFRRHQYFLMTDW
VGGVYGSPGLTGSRSGGLIAATWAALRSLGREGYLARAKAIFETAFDMQAAVRAIPELRVLGKPTFCFAFTSDAFDIYHV
NDFMRQRGWRFNGLQHPDALHMCVTGPQTQPGVAERFRQDLGEAVEHARHRAHARPQSSGVYGGDAAGLDLRDDARARAF
FTQVLDLFTDCPL
>Mature_493_residues
MRSIAARSSHAGAGLEQEGVMDLEEGVRQLYPYAAEFGALHEFPERGMPRERLLEELRSMAVREDRKWESGRCSGTMYCG
DHEHYAFLNEAYGLFSHVNALQRDLCPSMNRMESEIVAMTVALLHGEAVQRHDGAHRACGALSLGGTESILNATLAYREK
ARAERGIERPRMIWPASAHPAFRKAAHLFGFDVTVAPIDPVTMQVDADFVRDAVDANTVMLVGSACNYPYGTIDPIGALS
AIAVEKDVWLHVDGCLGGWMLPWGEALGYPDIPAFDFRLPGVTSISADTHKFGYGPKGGSVLAWRDASFRRHQYFLMTDW
VGGVYGSPGLTGSRSGGLIAATWAALRSLGREGYLARAKAIFETAFDMQAAVRAIPELRVLGKPTFCFAFTSDAFDIYHV
NDFMRQRGWRFNGLQHPDALHMCVTGPQTQPGVAERFRQDLGEAVEHARHRAHARPQSSGVYGGDAAGLDLRDDARARAF
FTQVLDLFTDCPL

Specific function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Possibly implicated in influencing the macrophage autophagy pathway [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=445, Percent_Identity=39.1011235955056, Blast_Score=324, Evalue=1e-88,
Organism=Escherichia coli, GI1789934, Length=337, Percent_Identity=24.9258160237389, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787769, Length=337, Percent_Identity=24.9258160237389, Blast_Score=99, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17557272, Length=419, Percent_Identity=38.1861575178998, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17543922, Length=428, Percent_Identity=35.7476635514019, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI25148342, Length=445, Percent_Identity=33.4831460674157, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6320500, Length=428, Percent_Identity=35.981308411215, Blast_Score=293, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6323905, Length=275, Percent_Identity=23.2727272727273, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355963, Length=400, Percent_Identity=42.5, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24654344, Length=400, Percent_Identity=42.5, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.2.27 [H]

Molecular weight: Translated: 54286; Mature: 54286

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIAARSSHAGAGLEQEGVMDLEEGVRQLYPYAAEFGALHEFPERGMPRERLLEELRSM
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AVREDRKWESGRCSGTMYCGDHEHYAFLNEAYGLFSHVNALQRDLCPSMNRMESEIVAMT
HHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VALLHGEAVQRHDGAHRACGALSLGGTESILNATLAYREKARAERGIERPRMIWPASAHP
HHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCH
AFRKAAHLFGFDVTVAPIDPVTMQVDADFVRDAVDANTVMLVGSACNYPYGTIDPIGALS
HHHHHHHHHCCEEEEECCCCEEEEECHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
AIAVEKDVWLHVDGCLGGWMLPWGEALGYPDIPAFDFRLPGVTSISADTHKFGYGPKGGS
HHEEECCEEEEECCCCCCCCCCCHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
VLAWRDASFRRHQYFLMTDWVGGVYGSPGLTGSRSGGLIAATWAALRSLGREGYLARAKA
EEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
IFETAFDMQAAVRAIPELRVLGKPTFCFAFTSDAFDIYHVNDFMRQRGWRFNGLQHPDAL
HHHHHHHHHHHHHHCCHHHCCCCCEEEEEEECCCEEEEEHHHHHHHCCCCCCCCCCCCCE
HMCVTGPQTQPGVAERFRQDLGEAVEHARHRAHARPQSSGVYGGDAAGLDLRDDARARAF
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
FTQVLDLFTDCPL
HHHHHHHHHCCCC
>Mature Secondary Structure
MRSIAARSSHAGAGLEQEGVMDLEEGVRQLYPYAAEFGALHEFPERGMPRERLLEELRSM
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AVREDRKWESGRCSGTMYCGDHEHYAFLNEAYGLFSHVNALQRDLCPSMNRMESEIVAMT
HHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VALLHGEAVQRHDGAHRACGALSLGGTESILNATLAYREKARAERGIERPRMIWPASAHP
HHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCH
AFRKAAHLFGFDVTVAPIDPVTMQVDADFVRDAVDANTVMLVGSACNYPYGTIDPIGALS
HHHHHHHHHCCEEEEECCCCEEEEECHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
AIAVEKDVWLHVDGCLGGWMLPWGEALGYPDIPAFDFRLPGVTSISADTHKFGYGPKGGS
HHEEECCEEEEECCCCCCCCCCCHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
VLAWRDASFRRHQYFLMTDWVGGVYGSPGLTGSRSGGLIAATWAALRSLGREGYLARAKA
EEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
IFETAFDMQAAVRAIPELRVLGKPTFCFAFTSDAFDIYHVNDFMRQRGWRFNGLQHPDAL
HHHHHHHHHHHHHHCCHHHCCCCCEEEEEEECCCEEEEEHHHHHHHCCCCCCCCCCCCCE
HMCVTGPQTQPGVAERFRQDLGEAVEHARHRAHARPQSSGVYGGDAAGLDLRDDARARAF
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
FTQVLDLFTDCPL
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA