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The map label for this gene is benM [H]

Identifier: 53722923

GI number: 53722923

Start: 2579291

End: 2580205

Strand: Reverse

Name: benM [H]

Synonym: BPSS1902

Alternate gene names: 53722923

Gene position: 2580205-2579291 (Counterclockwise)

Preceding gene: 53722928

Following gene: 53722921

Centisome position: 81.32

GC content: 66.01

Gene sequence:

>915_bases
ATGGAACTGCGGCATCTACGCTACTTCGTCGCGGTCGCGGAGGAGCAGAACTTCACGCGCGCGGCCGAGCGCCTGCACAT
CGCGCAGCCGCCGTTGAGCCGCCAGATCCAGCAGCTCGAGGAGGCGCTCGGCGTGATGCTGTTCGAGCGCCATGCGCGGC
CGCTGAAGATGACCGACGCGGGGCGCTTCTTCTATTCGCACGCGGTGCAATTGCTCGCGCAGACAGCCGAGCTCGAAGCG
ATGACGAAGCGCGTCGGCAAGATCGAGCGCGCGCTGTCGGTGGGCTTTGTCGGCTCGACGCTGTACGGGATGCTGCCGCG
CATCATCCGGCGCTTTCGCGCCGAGCACGGCGAAGTCGAGCTGAGCCTGCACGAGATGTCGACGATGGATCAGATCCAGG
CGCTCAAGGACGGGCGCATCGACGTCGGCTTCGGGCGCATTCGTCATGAGGATGCGCGCGTGCGGCGCGTCGTGCTGCGC
GAGGAGCGGATGATCGTCGCGCTGCCGGACGGGCATCCGCTCGAGGCGGCCAAGCCGGTGCTGTCGCTGCACGACCTCGT
CAACGAGACGCTGATCATCTTTCCGAAGTCGCCGCGGCCGAGCTACGCGGACCAGGTGCTCGCCGCGTTTCACGACCGCG
CGTTGAAGCCGCGCCGGATCTACGAGACGCGCGAGCTGCAGATCGCGCTCGGGCTCGTCGCGGCGGGCGAGGGCGTGTCC
GTCGTGCCGCACAGCGTGTACGGGCTCAAGCGCGACGACGTCGGCTACAAGGAGCTCGACGATCCGAACCTCGTGTCGCC
GATCATCATGAGCACGCGGATGCTCGACGAATCGGAGGACATTCGCGCGATGCTCGAGTTGATTTATCGTTTGTACGACG
AATTCAAGATGACGTATCTGCCGCCGCACGGATGA

Upstream 100 bases:

>100_bases
TAGTGCTCGACGACAGCGCGGTCAGATTGAGGATAGTTGCCGGCAAAACGGTGCGTCCAACACTGATTGAGTATCGGATC
GATATCCTCGAGGTATGAAG

Downstream 100 bases:

>100_bases
GCGGGAGGCGGGAAGCACGATGTCGCGGCGCGTGGCGGGGAAGCGCCGGCGTGCCGATGGCGCACGTTTCGCGCGCACGG
GGTGGGCGCGCGAGCGACGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MELRHLRYFVAVAEEQNFTRAAERLHIAQPPLSRQIQQLEEALGVMLFERHARPLKMTDAGRFFYSHAVQLLAQTAELEA
MTKRVGKIERALSVGFVGSTLYGMLPRIIRRFRAEHGEVELSLHEMSTMDQIQALKDGRIDVGFGRIRHEDARVRRVVLR
EERMIVALPDGHPLEAAKPVLSLHDLVNETLIIFPKSPRPSYADQVLAAFHDRALKPRRIYETRELQIALGLVAAGEGVS
VVPHSVYGLKRDDVGYKELDDPNLVSPIIMSTRMLDESEDIRAMLELIYRLYDEFKMTYLPPHG

Sequences:

>Translated_304_residues
MELRHLRYFVAVAEEQNFTRAAERLHIAQPPLSRQIQQLEEALGVMLFERHARPLKMTDAGRFFYSHAVQLLAQTAELEA
MTKRVGKIERALSVGFVGSTLYGMLPRIIRRFRAEHGEVELSLHEMSTMDQIQALKDGRIDVGFGRIRHEDARVRRVVLR
EERMIVALPDGHPLEAAKPVLSLHDLVNETLIIFPKSPRPSYADQVLAAFHDRALKPRRIYETRELQIALGLVAAGEGVS
VVPHSVYGLKRDDVGYKELDDPNLVSPIIMSTRMLDESEDIRAMLELIYRLYDEFKMTYLPPHG
>Mature_304_residues
MELRHLRYFVAVAEEQNFTRAAERLHIAQPPLSRQIQQLEEALGVMLFERHARPLKMTDAGRFFYSHAVQLLAQTAELEA
MTKRVGKIERALSVGFVGSTLYGMLPRIIRRFRAEHGEVELSLHEMSTMDQIQALKDGRIDVGFGRIRHEDARVRRVVLR
EERMIVALPDGHPLEAAKPVLSLHDLVNETLIIFPKSPRPSYADQVLAAFHDRALKPRRIYETRELQIALGLVAAGEGVS
VVPHSVYGLKRDDVGYKELDDPNLVSPIIMSTRMLDESEDIRAMLELIYRLYDEFKMTYLPPHG

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=268, Percent_Identity=34.3283582089552, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788887, Length=264, Percent_Identity=32.5757575757576, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI2367136, Length=264, Percent_Identity=31.0606060606061, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1790399, Length=259, Percent_Identity=30.1158301158301, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI145693105, Length=274, Percent_Identity=28.1021897810219, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787601, Length=249, Percent_Identity=28.1124497991968, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI157672245, Length=244, Percent_Identity=28.6885245901639, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081904, Length=265, Percent_Identity=28.6792452830189, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788297, Length=251, Percent_Identity=29.8804780876494, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787806, Length=249, Percent_Identity=28.9156626506024, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789204, Length=243, Percent_Identity=27.9835390946502, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790208, Length=146, Percent_Identity=35.6164383561644, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788481, Length=144, Percent_Identity=30.5555555555556, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=245, Percent_Identity=27.7551020408163, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787530, Length=250, Percent_Identity=26.4, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=28.6290322580645, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790783, Length=112, Percent_Identity=32.1428571428571, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786401, Length=174, Percent_Identity=32.7586206896552, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=139, Percent_Identity=33.8129496402878, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788748, Length=144, Percent_Identity=26.3888888888889, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789173, Length=280, Percent_Identity=25.7142857142857, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34756; Mature: 34756

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFVAVAEEQNFTRAAERLHIAQPPLSRQIQQLEEALGVMLFERHARPLKMTDA
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCC
GRFFYSHAVQLLAQTAELEAMTKRVGKIERALSVGFVGSTLYGMLPRIIRRFRAEHGEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LSLHEMSTMDQIQALKDGRIDVGFGRIRHEDARVRRVVLREERMIVALPDGHPLEAAKPV
EEHHHHHHHHHHHHHHCCCEEECCCHHCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHH
LSLHDLVNETLIIFPKSPRPSYADQVLAAFHDRALKPRRIYETRELQIALGLVAAGEGVS
HHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEEEECCCCCE
VVPHSVYGLKRDDVGYKELDDPNLVSPIIMSTRMLDESEDIRAMLELIYRLYDEFKMTYL
ECCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEC
PPHG
CCCC
>Mature Secondary Structure
MELRHLRYFVAVAEEQNFTRAAERLHIAQPPLSRQIQQLEEALGVMLFERHARPLKMTDA
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCC
GRFFYSHAVQLLAQTAELEAMTKRVGKIERALSVGFVGSTLYGMLPRIIRRFRAEHGEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LSLHEMSTMDQIQALKDGRIDVGFGRIRHEDARVRRVVLREERMIVALPDGHPLEAAKPV
EEHHHHHHHHHHHHHHCCCEEECCCHHCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHH
LSLHDLVNETLIIFPKSPRPSYADQVLAAFHDRALKPRRIYETRELQIALGLVAAGEGVS
HHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEEEECCCCCE
VVPHSVYGLKRDDVGYKELDDPNLVSPIIMSTRMLDESEDIRAMLELIYRLYDEFKMTYL
ECCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEC
PPHG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]