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The map label for this gene is thcD [H]

Identifier: 53722906

GI number: 53722906

Start: 2561180

End: 2562421

Strand: Reverse

Name: thcD [H]

Synonym: BPSS1885

Alternate gene names: 53722906

Gene position: 2562421-2561180 (Counterclockwise)

Preceding gene: 53722907

Following gene: 53722905

Centisome position: 80.76

GC content: 75.2

Gene sequence:

>1242_bases
ATGTCGACGGCTGACCCGTATCTGATCGCGGGCGGCGGGCACGCGGCGCGCCGCGCGGCCGAGACGCTTCGCGAGCGCGA
CGCGGCCGCGCGCATCGTGATGATCGGCGCGGAGCCCGAATTGCCGTACGACCGGCCCGTGTTGTCGAAGGAGGCGCTCG
TCGGCGGCGACGCTGGCGAGCGCCGCGCGTTCGTGCGCGATGCCGCGTGGTATCGCGAGCGCGACATCGAGCTGCGGCTC
GGCGTGCGCGTCGAGGCGATCGAGCGCGGCGCGCGGCGCGTGCGCCTGAGCGACGGCGCGCGCCTCGGCTACGCGCGGCT
GCTGATCGCGACCGGCTCGCGCGTGCGGCGCTTCGCGGGGCCCGTGGACGCCGGCGTGCACGTCCACTACGTGCGCACGC
TCGCCGATACGCGCGCATTGCGCGCGGCGCTCGCGCCGGGCAAGCGCGTCGCGGTGCTGGGCGGCGGCTTCATCGGGCTC
GAGGTCGCCGCGTCGGCGGTGCGGCTCGGGTGCCGCGCGACGGTTGTCGATCCCGCGCCGCGCCTGCTTCAGCGCGGGAT
GCCCGAGGCCGTCGGCGCGTTCGCGCTCGCGCTGCATGCGCGGCACGGCGTGGACGTGCGGCTCGGCGCGCTGCCGGAGC
GCATCCGCCGCGCCGCGAACGGCTCGGCGGTCGTCGAGACGAGCGCGGGCGGGATCGTCGCGGACGTCGTCGTCGCAGGC
ATCGGCGTCGTGCCGAACGTCGAGCTCGCGCAGGCGGCGGGCCTCGACGTCGACGACGGCGTCTGCGTCGACGAGATGTG
CCGCACCACCGATCCGGCGATCTTCGCGGCGGGGGAGGTCACGCGGCATTTCAATCCGCTCGTCGGCCGCGCTCTGCGGA
TCGAATCGTGGCAGATCGCGGAGAACCAGCCGGCCGTCGCGGCGGCGAACATGCTCGGCGCGGCCGAGACGTACGCGCAG
TGGCCTTGGCTATGGTCCGATCAGTACGACTGCAACCTGCAGACGCTCGGCCTTTTCGGCGGCGAGCAGACGCTCGTGCT
GCGCGGCTCGCCCGAGCGCGACCGCGCGTTCTGCGTGTTCGCGCTGAACGCGCGCCGCGAGCTGGCGGCGGTCGCGGCCG
TCAACGCGGGGCGCGAGATCGCAGTGTGCCGCCGGTTGATGACGGCGGGCGTCGCGCTCGATCCCGCGCGTCTCGCGGAT
CCGTCGGACGCGCTGCGCGCGATGCTGCCGCGCGGCGTGTAG

Upstream 100 bases:

>100_bases
GGGCTCTTCGATCTGCGCACCGGCGCGCCGAAATGCGCGCCGGTGACGCAGCCCGTACGCGCGTATCCGATTCGCATCGT
CGACGGGCAGGTCGAGATCC

Downstream 100 bases:

>100_bases
CGCGCGGCGCGTCGTGGCTGCCTGCCGCCTCTCGTTTCCCGCCGCCCGCATCGGGCGAGCGCGCCGCGCGGCGGATGGCC
GGGCGAGGTCGAGCACCGCG

Product: aromatic hydrocarbons catabolism-related reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSTADPYLIAGGGHAARRAAETLRERDAAARIVMIGAEPELPYDRPVLSKEALVGGDAGERRAFVRDAAWYRERDIELRL
GVRVEAIERGARRVRLSDGARLGYARLLIATGSRVRRFAGPVDAGVHVHYVRTLADTRALRAALAPGKRVAVLGGGFIGL
EVAASAVRLGCRATVVDPAPRLLQRGMPEAVGAFALALHARHGVDVRLGALPERIRRAANGSAVVETSAGGIVADVVVAG
IGVVPNVELAQAAGLDVDDGVCVDEMCRTTDPAIFAAGEVTRHFNPLVGRALRIESWQIAENQPAVAAANMLGAAETYAQ
WPWLWSDQYDCNLQTLGLFGGEQTLVLRGSPERDRAFCVFALNARRELAAVAAVNAGREIAVCRRLMTAGVALDPARLAD
PSDALRAMLPRGV

Sequences:

>Translated_413_residues
MSTADPYLIAGGGHAARRAAETLRERDAAARIVMIGAEPELPYDRPVLSKEALVGGDAGERRAFVRDAAWYRERDIELRL
GVRVEAIERGARRVRLSDGARLGYARLLIATGSRVRRFAGPVDAGVHVHYVRTLADTRALRAALAPGKRVAVLGGGFIGL
EVAASAVRLGCRATVVDPAPRLLQRGMPEAVGAFALALHARHGVDVRLGALPERIRRAANGSAVVETSAGGIVADVVVAG
IGVVPNVELAQAAGLDVDDGVCVDEMCRTTDPAIFAAGEVTRHFNPLVGRALRIESWQIAENQPAVAAANMLGAAETYAQ
WPWLWSDQYDCNLQTLGLFGGEQTLVLRGSPERDRAFCVFALNARRELAAVAAVNAGREIAVCRRLMTAGVALDPARLAD
PSDALRAMLPRGV
>Mature_412_residues
STADPYLIAGGGHAARRAAETLRERDAAARIVMIGAEPELPYDRPVLSKEALVGGDAGERRAFVRDAAWYRERDIELRLG
VRVEAIERGARRVRLSDGARLGYARLLIATGSRVRRFAGPVDAGVHVHYVRTLADTRALRAALAPGKRVAVLGGGFIGLE
VAASAVRLGCRATVVDPAPRLLQRGMPEAVGAFALALHARHGVDVRLGALPERIRRAANGSAVVETSAGGIVADVVVAGI
GVVPNVELAQAAGLDVDDGVCVDEMCRTTDPAIFAAGEVTRHFNPLVGRALRIESWQIAENQPAVAAANMLGAAETYAQW
PWLWSDQYDCNLQTLGLFGGEQTLVLRGSPERDRAFCVFALNARRELAAVAAVNAGREIAVCRRLMTAGVALDPARLADP
SDALRAMLPRGV

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=399, Percent_Identity=29.8245614035088, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI65787454, Length=399, Percent_Identity=29.8245614035088, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI226437568, Length=399, Percent_Identity=29.8245614035088, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI22202629, Length=381, Percent_Identity=28.3464566929134, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4757732, Length=382, Percent_Identity=28.7958115183246, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI195927006, Length=187, Percent_Identity=31.5508021390374, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788892, Length=405, Percent_Identity=36.5432098765432, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1789065, Length=274, Percent_Identity=28.8321167883212, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789765, Length=326, Percent_Identity=27.9141104294479, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17559934, Length=404, Percent_Identity=27.7227722772277, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32564386, Length=347, Percent_Identity=29.1066282420749, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24639257, Length=404, Percent_Identity=27.970297029703, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI281359715, Length=404, Percent_Identity=27.970297029703, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI281359713, Length=404, Percent_Identity=27.970297029703, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24639250, Length=404, Percent_Identity=27.970297029703, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI18543267, Length=404, Percent_Identity=27.970297029703, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24639252, Length=404, Percent_Identity=27.970297029703, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24585130, Length=406, Percent_Identity=22.9064039408867, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581020, Length=384, Percent_Identity=27.34375, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28573993, Length=384, Percent_Identity=27.34375, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43901; Mature: 43770

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTADPYLIAGGGHAARRAAETLRERDAAARIVMIGAEPELPYDRPVLSKEALVGGDAGE
CCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHEECCCCCC
RRAFVRDAAWYRERDIELRLGVRVEAIERGARRVRLSDGARLGYARLLIATGSRVRRFAG
HHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEECCCCCCCCEEEEECCCHHHHHHCC
PVDAGVHVHYVRTLADTRALRAALAPGKRVAVLGGGFIGLEVAASAVRLGCRATVVDPAP
CCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCH
RLLQRGMPEAVGAFALALHARHGVDVRLGALPERIRRAANGSAVVETSAGGIVADVVVAG
HHHHCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCEEEEECCCCHHHHHHHHC
IGVVPNVELAQAAGLDVDDGVCVDEMCRTTDPAIFAAGEVTRHFNPLVGRALRIESWQIA
CCCCCCCHHHHHCCCCCCCCCCHHHHHCCCCCCEEECCHHHHHCCHHHCCEEEECCEEEC
ENQPAVAAANMLGAAETYAQWPWLWSDQYDCNLQTLGLFGGEQTLVLRGSPERDRAFCVF
CCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCEEEEE
ALNARRELAAVAAVNAGREIAVCRRLMTAGVALDPARLADPSDALRAMLPRGV
EECCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
STADPYLIAGGGHAARRAAETLRERDAAARIVMIGAEPELPYDRPVLSKEALVGGDAGE
CCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHEECCCCCC
RRAFVRDAAWYRERDIELRLGVRVEAIERGARRVRLSDGARLGYARLLIATGSRVRRFAG
HHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEECCCCCCCCEEEEECCCHHHHHHCC
PVDAGVHVHYVRTLADTRALRAALAPGKRVAVLGGGFIGLEVAASAVRLGCRATVVDPAP
CCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCH
RLLQRGMPEAVGAFALALHARHGVDVRLGALPERIRRAANGSAVVETSAGGIVADVVVAG
HHHHCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCEEEEECCCCHHHHHHHHC
IGVVPNVELAQAAGLDVDDGVCVDEMCRTTDPAIFAAGEVTRHFNPLVGRALRIESWQIA
CCCCCCCHHHHHCCCCCCCCCCHHHHHCCCCCCEEECCHHHHHCCHHHCCEEEECCEEEC
ENQPAVAAANMLGAAETYAQWPWLWSDQYDCNLQTLGLFGGEQTLVLRGSPERDRAFCVF
CCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCEEEEE
ALNARRELAAVAAVNAGREIAVCRRLMTAGVALDPARLADPSDALRAMLPRGV
EECCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]