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The map label for this gene is amiD [H]

Identifier: 53722859

GI number: 53722859

Start: 2501901

End: 2502935

Strand: Reverse

Name: amiD [H]

Synonym: BPSS1840

Alternate gene names: 53722859

Gene position: 2502935-2501901 (Counterclockwise)

Preceding gene: 53722860

Following gene: 53722858

Centisome position: 78.88

GC content: 68.79

Gene sequence:

>1035_bases
ATGGCTGAAGAACAACAAGCGAAAATCATCAACGAGGGCAGGCGCGGGTTTCTCGCGCGTTCGTCGGCGTGGCCGGTGGG
GATGCTGATGCTGCCGCTGGCCGGCTGCGGCGACGGCGACGGCGTCGTGCCGTCCGCGCACGCGCAGGATACGCCCCCGC
CGTCGGGCGCCGGTTACACGATCGATCGCTCGATCCAGTCGCCGAACCAGGATTCGCGGGTGCGCACGCTCGTGCTGCAT
TACACCGCGCAGACGCTCGCGGATTCGATCGCCTCGCTGACGAGCCCGCAGCGGCAGGTCAGCGCGCACTATCTGGTGCC
CGACGCGGCCGACGACGGCCGGCGCTTCAAGGTATTCGAGCTCGTGCCGCAGGCGCGCCGCGCGTGGCACGCGGGCGTCA
GCTACTGGCAAGGCGACCGGATGCTCAATGCGGGCTCGGTCGGCATCGAGATCGTCAATCTCGGCTTTCCGCCGGAGGAC
GAAAACCTGCCGCTGATGAACCGGCGCTGGTATCCGTATCCGGACGCGCAGGTGGCCGTGTTCGGCGCGCTGGCGGCCGA
TGTCGTCGCGCAGCATCAGGTGCTGCCGCACAAGGTGGTCGGGCACTCGGACGTCGCGCCGGGCCGCAAGACCGATCCGG
GGCCGCTGTTCCCGTGGAAGAAGCTCTACGATCAATACAAGATCGGCGCATGGCCGGAGGCGGAGGCCGTCGATTACTAC
CGCGTCAACCGGCCGTTCGCGGGCGACGTCGCGAGCCTGCAGTCGAAGCTGCTCGCGTACGGCTACGACACGCCGCAGAC
GGGCACGCTCGACGCGCAGACCGTCAACGTCGTGTCGGCGTTCCAGATGCATTTCAGGCCGTCGCGTTACGACGGCGTGC
CGGATGTCGAGACCGTCGCGATTCTCGACGCGCTGCTCGAGAAGTACTTCAACCGCGGCCGCGCGATCAACAAGCAGACG
CTGCCGGGCGCAACCGCGCCGACGGGCGAGAAGGGCAGCGACGCGTGGCCGCTCGCGCCCGCGCCGTCGCGCTGA

Upstream 100 bases:

>100_bases
GCGGACTCCCACGCTCGCGCGTCGCATCATCGCGCAACCTACTCGCTCGGCGCATCGCGCCGATTCTCAATCGCAAGTTA
CGACTCGAACGGAAAAGGGT

Downstream 100 bases:

>100_bases
CGTCGCCGGCGCCCGCGCGCCTTGCTGCGCCGCGAGACGGCGCAGCAAGGCCCGGTTGCGTTCGCGCTTGACCGCTTCGC
GCCTTCGCTTGCCCGCCTCG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAEEQQAKIINEGRRGFLARSSAWPVGMLMLPLAGCGDGDGVVPSAHAQDTPPPSGAGYTIDRSIQSPNQDSRVRTLVLH
YTAQTLADSIASLTSPQRQVSAHYLVPDAADDGRRFKVFELVPQARRAWHAGVSYWQGDRMLNAGSVGIEIVNLGFPPED
ENLPLMNRRWYPYPDAQVAVFGALAADVVAQHQVLPHKVVGHSDVAPGRKTDPGPLFPWKKLYDQYKIGAWPEAEAVDYY
RVNRPFAGDVASLQSKLLAYGYDTPQTGTLDAQTVNVVSAFQMHFRPSRYDGVPDVETVAILDALLEKYFNRGRAINKQT
LPGATAPTGEKGSDAWPLAPAPSR

Sequences:

>Translated_344_residues
MAEEQQAKIINEGRRGFLARSSAWPVGMLMLPLAGCGDGDGVVPSAHAQDTPPPSGAGYTIDRSIQSPNQDSRVRTLVLH
YTAQTLADSIASLTSPQRQVSAHYLVPDAADDGRRFKVFELVPQARRAWHAGVSYWQGDRMLNAGSVGIEIVNLGFPPED
ENLPLMNRRWYPYPDAQVAVFGALAADVVAQHQVLPHKVVGHSDVAPGRKTDPGPLFPWKKLYDQYKIGAWPEAEAVDYY
RVNRPFAGDVASLQSKLLAYGYDTPQTGTLDAQTVNVVSAFQMHFRPSRYDGVPDVETVAILDALLEKYFNRGRAINKQT
LPGATAPTGEKGSDAWPLAPAPSR
>Mature_343_residues
AEEQQAKIINEGRRGFLARSSAWPVGMLMLPLAGCGDGDGVVPSAHAQDTPPPSGAGYTIDRSIQSPNQDSRVRTLVLHY
TAQTLADSIASLTSPQRQVSAHYLVPDAADDGRRFKVFELVPQARRAWHAGVSYWQGDRMLNAGSVGIEIVNLGFPPEDE
NLPLMNRRWYPYPDAQVAVFGALAADVVAQHQVLPHKVVGHSDVAPGRKTDPGPLFPWKKLYDQYKIGAWPEAEAVDYYR
VNRPFAGDVASLQSKLLAYGYDTPQTGTLDAQTVNVVSAFQMHFRPSRYDGVPDVETVAILDALLEKYFNRGRAINKQTL
PGATAPTGEKGSDAWPLAPAPSR

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=278, Percent_Identity=41.0071942446043, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1786300, Length=124, Percent_Identity=34.6774193548387, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 37544; Mature: 37412

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEQQAKIINEGRRGFLARSSAWPVGMLMLPLAGCGDGDGVVPSAHAQDTPPPSGAGYT
CCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEE
IDRSIQSPNQDSRVRTLVLHYTAQTLADSIASLTSPQRQVSAHYLVPDAADDGRRFKVFE
EECCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCCEEEHHH
LVPQARRAWHAGVSYWQGDRMLNAGSVGIEIVNLGFPPEDENLPLMNRRWYPYPDAQVAV
HHHHHHHHHHHCCHHCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHH
FGALAADVVAQHQVLPHKVVGHSDVAPGRKTDPGPLFPWKKLYDQYKIGAWPEAEAVDYY
HHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEE
RVNRPFAGDVASLQSKLLAYGYDTPQTGTLDAQTVNVVSAFQMHFRPSRYDGVPDVETVA
ECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
ILDALLEKYFNRGRAINKQTLPGATAPTGEKGSDAWPLAPAPSR
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEEQQAKIINEGRRGFLARSSAWPVGMLMLPLAGCGDGDGVVPSAHAQDTPPPSGAGYT
CCHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEE
IDRSIQSPNQDSRVRTLVLHYTAQTLADSIASLTSPQRQVSAHYLVPDAADDGRRFKVFE
EECCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCCEEEHHH
LVPQARRAWHAGVSYWQGDRMLNAGSVGIEIVNLGFPPEDENLPLMNRRWYPYPDAQVAV
HHHHHHHHHHHCCHHCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHH
FGALAADVVAQHQVLPHKVVGHSDVAPGRKTDPGPLFPWKKLYDQYKIGAWPEAEAVDYY
HHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEE
RVNRPFAGDVASLQSKLLAYGYDTPQTGTLDAQTVNVVSAFQMHFRPSRYDGVPDVETVA
ECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
ILDALLEKYFNRGRAINKQTLPGATAPTGEKGSDAWPLAPAPSR
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]