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The map label for this gene is potH [H]

Identifier: 53722757

GI number: 53722757

Start: 2383826

End: 2385094

Strand: Reverse

Name: potH [H]

Synonym: BPSS1736

Alternate gene names: 53722757

Gene position: 2385094-2383826 (Counterclockwise)

Preceding gene: 53722758

Following gene: 53722756

Centisome position: 75.17

GC content: 67.14

Gene sequence:

>1269_bases
TTGAATACGATGACGATCGCTACGCAGTCGGCGCCGCCGACTCAATCGCTCAGGCGTGAACTGAAAGCCGCCGAGGCCAG
GCGGCGCACGATGGCGCTGCTGCTCGTCGCGCCGCTCGCGATATTCCTGCTGCTCGTGTTCGTCGTGCCGATCGGCGCGT
TGCTCACGCGCGCGGTGCAGAATCCCGAGATCGCGACTGCGCTGCCGCGCACCGTGGCCGCGCTGTCGAACTGGGACCGC
AAGGCGGCGCCCGCCGACGCGGCGTACGCGGCGCTCGCCGCCGATCTCACGCAGATCGCCGACGGCGAGGCGATGGGCGC
GCTCGCGCGACGCCTGAACACCGAGATACCCGGCTATCGGTCGCTCGTCGCGAAGACCGCGCGCGCGATGCCGCTGACGG
GCGACGCGAACGCGCCGCTCGCGCCGGCGCAGACGCGTGCGAAGCTGATCGAGCTCGACGAGCGCTGGGGCGACGCCGCG
TACTGGCAGGCGATCGCGAAGAACGGCGGCCGCTATTCGCCGTTCTACCTGCTCGCGGCGCTCGATCACAAGCAGGACGG
TTTCGGCAGCATCGTGCCCGCCGATCCCGATCAATCGATCTACCTCGCGGTGTTCGGCCGCACGCTGCTGATCGGCTTCG
CGGTCACGCTGTTCGCGCTCGCGCTCGGCTATCCGCTCGCGTACTGGATCTCGACGCTGCCCGAGCGGCGCGCGAATCTC
GCGATGATCCTCGTGCTGATTCCGTTCTGGACGTCGGTGCTCGTGCGCGTCGCCGCCTGGATCGTGCTGCTGCAAAGCGA
AGGGCTCGTCAATCGCGCGCTGATCGACACCGGACTGATTTCGCAGCCGCTCGCGCTGTTGTTCAATCGCGTCGGCGTCT
ACATCTCGATGACGCACATCCTGCTGCCGTTCATGATCCTGCCGCTCTACAGCGTGATGAAATCGATTCCGCCGAGCTAT
CAGCGCGCGGCGGTGTCGCTCGGCAGCCATCCGTTCGCGGCGTTCTGGCGCGTGTACGTGCCGCAGACCTATCCGGGCGT
CGGCGCGGGCGCGCTGCTCGTCTTCATTCTCGCGATCGGCTACTACATCACGCCCGCGTTGCTCGGCGGTCCGAACGATC
AGATGGTCAGCTACTACGTTGCGTACTTCACGAACGTGACGATCAACTGGGGCATGGCGTGTGCGCTCGGCGGCCTGCTG
CTCGCCGCGACGCTCGTGCTGTATGCGATCTACGGCCGCTTTACGCGCTCGCAATTGAGCCTCGGCTGA

Upstream 100 bases:

>100_bases
TCGAAGTGACGGCCGCGTGAGCCGGTGAAGCAAACGGCCGCGCGCGCCGTGGCGGCGTGCGCGGCGTTCCTCCGAACGGC
GTTTCGGCCGGAGACAACCG

Downstream 100 bases:

>100_bases
ATGAGCGCCGAGCGAACAGGGGAAACGAACATGAAACTGCTGGCGAAGCCGCTCTTCGCGCCGCACATGTCGGGGCTCGA
GCGCGCCTGGTATTTCGCGC

Product: ABC transport system, membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNTMTIATQSAPPTQSLRRELKAAEARRRTMALLLVAPLAIFLLLVFVVPIGALLTRAVQNPEIATALPRTVAALSNWDR
KAAPADAAYAALAADLTQIADGEAMGALARRLNTEIPGYRSLVAKTARAMPLTGDANAPLAPAQTRAKLIELDERWGDAA
YWQAIAKNGGRYSPFYLLAALDHKQDGFGSIVPADPDQSIYLAVFGRTLLIGFAVTLFALALGYPLAYWISTLPERRANL
AMILVLIPFWTSVLVRVAAWIVLLQSEGLVNRALIDTGLISQPLALLFNRVGVYISMTHILLPFMILPLYSVMKSIPPSY
QRAAVSLGSHPFAAFWRVYVPQTYPGVGAGALLVFILAIGYYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGGLL
LAATLVLYAIYGRFTRSQLSLG

Sequences:

>Translated_422_residues
MNTMTIATQSAPPTQSLRRELKAAEARRRTMALLLVAPLAIFLLLVFVVPIGALLTRAVQNPEIATALPRTVAALSNWDR
KAAPADAAYAALAADLTQIADGEAMGALARRLNTEIPGYRSLVAKTARAMPLTGDANAPLAPAQTRAKLIELDERWGDAA
YWQAIAKNGGRYSPFYLLAALDHKQDGFGSIVPADPDQSIYLAVFGRTLLIGFAVTLFALALGYPLAYWISTLPERRANL
AMILVLIPFWTSVLVRVAAWIVLLQSEGLVNRALIDTGLISQPLALLFNRVGVYISMTHILLPFMILPLYSVMKSIPPSY
QRAAVSLGSHPFAAFWRVYVPQTYPGVGAGALLVFILAIGYYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGGLL
LAATLVLYAIYGRFTRSQLSLG
>Mature_422_residues
MNTMTIATQSAPPTQSLRRELKAAEARRRTMALLLVAPLAIFLLLVFVVPIGALLTRAVQNPEIATALPRTVAALSNWDR
KAAPADAAYAALAADLTQIADGEAMGALARRLNTEIPGYRSLVAKTARAMPLTGDANAPLAPAQTRAKLIELDERWGDAA
YWQAIAKNGGRYSPFYLLAALDHKQDGFGSIVPADPDQSIYLAVFGRTLLIGFAVTLFALALGYPLAYWISTLPERRANL
AMILVLIPFWTSVLVRVAAWIVLLQSEGLVNRALIDTGLISQPLALLFNRVGVYISMTHILLPFMILPLYSVMKSIPPSY
QRAAVSLGSHPFAAFWRVYVPQTYPGVGAGALLVFILAIGYYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGGLL
LAATLVLYAIYGRFTRSQLSLG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=228, Percent_Identity=35.0877192982456, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787369, Length=223, Percent_Identity=31.8385650224215, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787713, Length=180, Percent_Identity=31.6666666666667, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 45658; Mature: 45658

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTMTIATQSAPPTQSLRRELKAAEARRRTMALLLVAPLAIFLLLVFVVPIGALLTRAVQ
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPEIATALPRTVAALSNWDRKAAPADAAYAALAADLTQIADGEAMGALARRLNTEIPGYR
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHH
SLVAKTARAMPLTGDANAPLAPAQTRAKLIELDERWGDAAYWQAIAKNGGRYSPFYLLAA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEE
LDHKQDGFGSIVPADPDQSIYLAVFGRTLLIGFAVTLFALALGYPLAYWISTLPERRANL
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMILVLIPFWTSVLVRVAAWIVLLQSEGLVNRALIDTGLISQPLALLFNRVGVYISMTHI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPFMILPLYSVMKSIPPSYQRAAVSLGSHPFAAFWRVYVPQTYPGVGAGALLVFILAIG
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
YYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGGLLLAATLVLYAIYGRFTRSQLS
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LG
CC
>Mature Secondary Structure
MNTMTIATQSAPPTQSLRRELKAAEARRRTMALLLVAPLAIFLLLVFVVPIGALLTRAVQ
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPEIATALPRTVAALSNWDRKAAPADAAYAALAADLTQIADGEAMGALARRLNTEIPGYR
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHH
SLVAKTARAMPLTGDANAPLAPAQTRAKLIELDERWGDAAYWQAIAKNGGRYSPFYLLAA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEE
LDHKQDGFGSIVPADPDQSIYLAVFGRTLLIGFAVTLFALALGYPLAYWISTLPERRANL
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMILVLIPFWTSVLVRVAAWIVLLQSEGLVNRALIDTGLISQPLALLFNRVGVYISMTHI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPFMILPLYSVMKSIPPSYQRAAVSLGSHPFAAFWRVYVPQTYPGVGAGALLVFILAIG
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
YYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGGLLLAATLVLYAIYGRFTRSQLS
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]