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The map label for this gene is potD-A [H]

Identifier: 53722758

GI number: 53722758

Start: 2385186

End: 2386232

Strand: Reverse

Name: potD-A [H]

Synonym: BPSS1737

Alternate gene names: 53722758

Gene position: 2386232-2385186 (Counterclockwise)

Preceding gene: 53722759

Following gene: 53722757

Centisome position: 75.2

GC content: 65.23

Gene sequence:

>1047_bases
ATGCACCACACGTTCAACACGCCGCGCCGCGCGGTTGCGCTGCTGTCGCTCGCCGCGTTCTGCGCGACGGCCGGCGCGGC
CGAGCTGACCGTCGTCAATTTCGGCGGCGCGAACGGCGACGCGCAGAAGGCCGCGTTCAACCAGCCGTTCGAGAAGGCGA
CCGGCAACAAGGTGACGGCCGTCGAATACAACGGCGAGCAGGCGAAAGTGAAGGCGATGGTCGAGGCGAAGCACGTGAAC
TGGGACGTCGTCGAAGTCGAGTCGGGCGACCTGAACCGCGGCTGCGACGAAGGCCTGTACGAGAAACTCGACTGGGCGAA
GATCGCGAAGAAGTCCGATCTGATTGCCGAATCGCCGCAAACCTGCGGCGTCGGTTTCTTCGTGTGGTCGACCGCGCTTG
CATACGACGCGGACAAGCTCAAGCGCGCGCCCGCCGGCTGGGCCGATTTCTGGGACGTGAAGAAATTCCCGGGCAAGCGC
GGGATGCGCAAGGGCGCGCGCTACAACCTCGAGTTCGCGCTGATGGCGGACGGCGTCGCGCCGAAGGACGTCTACAAGGT
GCTCGCGACGAAGGCGGGCCAGGATCGCGCATTCAAGAAGCTCGACGAGCTGAAGCCGAACATCCAGTGGTGGGAAGCGG
GCGCGCAGCCGCCGCAGTTCCTCGTCGCGGGCGACGTCGTGATGTCGACCGCGTACAACGGCCGCATCAGCGCCGCGCAG
AAGGAAGGCAAGAACCTGAAGGTGGTGTGGAGCGGCAGCATCTACGATCTGGACTACTGGGCGATTCCGAAGGGCACGCC
GAACAAGGCGCTCGCCGAGAAGTACATCGCCTACACGCTGTCGTCGAAGCCGCAGCAGGATTACGCGCAACACATCGCGT
ACGGCCCCGCGAATGTCGCCGCGATCAAGGCGCTCGACGCGAAGACGCTCGCGAACCTGCCGAACTCGCCGGCCAACGGC
AAGAACGCGGTGCTGCAGAACATCACGTTCTGGACCGATCACGGCGACGAGCTCGAGCAGCGCTTTGCGGCATGGGCGTC
GAAGTGA

Upstream 100 bases:

>100_bases
CCGAGCATCTGCGGGTCTTCGCATGAACGCGAAGCCGCGCGTGGCGCGCCGCGCTTCGTATAACCACCACGCTTCATCAT
CCGTCACGAGAGGAAACATC

Downstream 100 bases:

>100_bases
CGGCCGCGTGAGCCGGTGAAGCAAACGGCCGCGCGCGCCGTGGCGGCGTGCGCGGCGTTCCTCCGAACGGCGTTTCGGCC
GGAGACAACCGTTGAATACG

Product: ABC transport system, exported protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MHHTFNTPRRAVALLSLAAFCATAGAAELTVVNFGGANGDAQKAAFNQPFEKATGNKVTAVEYNGEQAKVKAMVEAKHVN
WDVVEVESGDLNRGCDEGLYEKLDWAKIAKKSDLIAESPQTCGVGFFVWSTALAYDADKLKRAPAGWADFWDVKKFPGKR
GMRKGARYNLEFALMADGVAPKDVYKVLATKAGQDRAFKKLDELKPNIQWWEAGAQPPQFLVAGDVVMSTAYNGRISAAQ
KEGKNLKVVWSGSIYDLDYWAIPKGTPNKALAEKYIAYTLSSKPQQDYAQHIAYGPANVAAIKALDAKTLANLPNSPANG
KNAVLQNITFWTDHGDELEQRFAAWASK

Sequences:

>Translated_348_residues
MHHTFNTPRRAVALLSLAAFCATAGAAELTVVNFGGANGDAQKAAFNQPFEKATGNKVTAVEYNGEQAKVKAMVEAKHVN
WDVVEVESGDLNRGCDEGLYEKLDWAKIAKKSDLIAESPQTCGVGFFVWSTALAYDADKLKRAPAGWADFWDVKKFPGKR
GMRKGARYNLEFALMADGVAPKDVYKVLATKAGQDRAFKKLDELKPNIQWWEAGAQPPQFLVAGDVVMSTAYNGRISAAQ
KEGKNLKVVWSGSIYDLDYWAIPKGTPNKALAEKYIAYTLSSKPQQDYAQHIAYGPANVAAIKALDAKTLANLPNSPANG
KNAVLQNITFWTDHGDELEQRFAAWASK
>Mature_348_residues
MHHTFNTPRRAVALLSLAAFCATAGAAELTVVNFGGANGDAQKAAFNQPFEKATGNKVTAVEYNGEQAKVKAMVEAKHVN
WDVVEVESGDLNRGCDEGLYEKLDWAKIAKKSDLIAESPQTCGVGFFVWSTALAYDADKLKRAPAGWADFWDVKKFPGKR
GMRKGARYNLEFALMADGVAPKDVYKVLATKAGQDRAFKKLDELKPNIQWWEAGAQPPQFLVAGDVVMSTAYNGRISAAQ
KEGKNLKVVWSGSIYDLDYWAIPKGTPNKALAEKYIAYTLSSKPQQDYAQHIAYGPANVAAIKALDAKTLANLPNSPANG
KNAVLQNITFWTDHGDELEQRFAAWASK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=274, Percent_Identity=25.5474452554745, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38010; Mature: 38010

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHTFNTPRRAVALLSLAAFCATAGAAELTVVNFGGANGDAQKAAFNQPFEKATGNKVTA
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCHHHCCCCEEEE
VEYNGEQAKVKAMVEAKHVNWDVVEVESGDLNRGCDEGLYEKLDWAKIAKKSDLIAESPQ
EEECCCHHEEEEEEEHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
TCGVGFFVWSTALAYDADKLKRAPAGWADFWDVKKFPGKRGMRKGARYNLEFALMADGVA
CCCCHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCC
PKDVYKVLATKAGQDRAFKKLDELKPNIQWWEAGAQPPQFLVAGDVVMSTAYNGRISAAQ
HHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEECHHHHHHCCCCCEEHHH
KEGKNLKVVWSGSIYDLDYWAIPKGTPNKALAEKYIAYTLSSKPQQDYAQHIAYGPANVA
HCCCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHH
AIKALDAKTLANLPNSPANGKNAVLQNITFWTDHGDELEQRFAAWASK
HHHHHHHHHHHCCCCCCCCCCHHHHHEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MHHTFNTPRRAVALLSLAAFCATAGAAELTVVNFGGANGDAQKAAFNQPFEKATGNKVTA
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCHHHCCCCEEEE
VEYNGEQAKVKAMVEAKHVNWDVVEVESGDLNRGCDEGLYEKLDWAKIAKKSDLIAESPQ
EEECCCHHEEEEEEEHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
TCGVGFFVWSTALAYDADKLKRAPAGWADFWDVKKFPGKRGMRKGARYNLEFALMADGVA
CCCCHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCC
PKDVYKVLATKAGQDRAFKKLDELKPNIQWWEAGAQPPQFLVAGDVVMSTAYNGRISAAQ
HHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEECHHHHHHCCCCCEEHHH
KEGKNLKVVWSGSIYDLDYWAIPKGTPNKALAEKYIAYTLSSKPQQDYAQHIAYGPANVA
HCCCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHH
AIKALDAKTLANLPNSPANGKNAVLQNITFWTDHGDELEQRFAAWASK
HHHHHHHHHHHCCCCCCCCCCHHHHHEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]