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The map label for this gene is hyfB [H]

Identifier: 53722175

GI number: 53722175

Start: 1533817

End: 1535823

Strand: Reverse

Name: hyfB [H]

Synonym: BPSS1147

Alternate gene names: 53722175

Gene position: 1535823-1533817 (Counterclockwise)

Preceding gene: 53722182

Following gene: 53722174

Centisome position: 48.4

GC content: 69.71

Gene sequence:

>2007_bases
ATGGAACAGGGGCTGCTTCTCCGATGCGTGCTGTTCGTTGCGCTCGCGTGGCTCGCGCTCGGCTGCGCCGGACTCGCGAA
CCTGCGCCGCACCGGTGTGATCGCACACGGCCTCTTCCCGCTCGGCGCGCTGGCCGGGCTCGTGCTCGCGGCGCTCGGCC
TGGCCGGCGTGTTCGCCGGGCCGTCGAGCGCCGTGCTGCCGCTCGGGCTGCCCGACCTGCCGTTCCATCTGCGTGTCGAT
CCGCTGTCCGCGTACTTCCTGTTCGTGCTCGGCGTGGTGACGGCGGGCGTCGGCGCGTTCTCGTCCGGCTACTTCCGCAA
GGGCGAGGGCACGGCGCCCGGCCTCATCTGCTTCGAGTACCACGTGTGCGTCGCGAGCATGGTGCTGCTGCTCGTCGCGG
ACGATGCGTACTGCTTCATGGTCGCGTGGGAGACGCTGACGCTGTCGGCGACCTTCCTCGTGATGACGAACCACCGGATC
GCCGAGATCCGCCGCGCCGCATTCGTGTATTTCCTGATCTCGCACGTCGGCGCGCTCGCGCTGCTGCTGTGCTTCGGCCT
GCTGCAGGCGGGCACGGGCGACTACACGTTCGCGAACATGCGCATCCAGCATCTCGACGGGCTCGCCGCATCGGCCGCGT
TCGTGCTCGCGCTCGTCGGCTTCGGCGCGAAAGCCGGCATCTTTCCGCTGCACGTCTGGCTGCCGGACGCGCATCCGGCT
GCGCCGTCGCCGGTATCCGCGCTGCTGAGCGGCTTCGTGCTGAAGGTCGGCCTGTATGGCATATTGCGCACGGTGCTCGA
TCTGCTGCACGTGCAGGTGGCGTGGTGGGGCGTGCTCATGCTCGCGCTCGGCCTGTTCGGCGCGTTGCTCGGCGCCGTGT
TCAGCACGATCCAGACCGACATGAAGCGGCTGCTCGCCTATTCGTCGATCGACAACGTCGGCCTGATGTGCGTGAGCCTC
GGGCTCACGATCCTGTTCCTCGGCTACCGGCTGCCCTCGCTCGCCGCGCTGTCGCTGACGGCGCTGCTGTATCAGATCGT
CGCGCACGCGTGCTTCAAGAGCCTGCTGTTCCTCGGCACGGGCGCCGTGCTGCACGCGACGGGCGAGCGCAATCTCGGCC
GGCTCGGCGGCTTGATCCGCTTCATGCCGTGGACCGCGTGGGCGATGCTCGTCGGCGTCGCCGCGAGCGCCGGGCTGCCG
CCGTCGAGCGGCTTCGTGTCCGAATGGCTGCTCGTGCAGAGCTTCCTGTTCACGCCGGGGCTGCCGGATTCGGTGCTCGG
GATGACGGTGCCGCTCGTCGCGGCGCTCGTCGCGCTGACGGCGGCGCTCGCCGGCTACGCGATGGTCAAGTTCTTCGGCA
TCGTGTTCCTCGGCCGGCCGCGCGAGGCCAAGCTCGAGCGCGCGCGCGACGCGAGCCCGTGGGAGCGGATCGCGTTCGGC
TGGTTCGCGGCCGCGAGCGTGCTGCTCGGCCTCGCGCCCGTTCAGTACGTGAAGGTGCTCGATGCGCTCACGCGCGCGCT
CGTCGGCGCGGGCATCGCGGGCACGAATCACGGGTGGCTGCTGTTCGCGCCCGTGTCCACCGCGCGCGCGAGCTACATGC
CGGCGCTCTTCATGCTGTTCTTCGTCGCGTGCTGCGCGCTCGCGTGGCTGCTCGTGCGGCGCTTCTATCACGGCAGGCTG
CGGCGCGCGGCGCCGTGGTCGTGCGGCAATCCGTTCGTGACCGCGCGCATGCAGGACACGGCCGAAGGCTTCGGCCAGCC
GATCCGCGAGATCTTCACGCCGTTCTTCCGGATCGAGCGGCAACTGCCGTCGCCGTTCGATCGGCAGCCGGCCTACCGGA
TGAGCGTCGCGGACCGCACGTGGCACCTGCTGTACGAGCCGATCGCCGCGCTCGCAAACCGCGTCGCCGCGCTCGTCGGG
CGGTTGCAGACGGGCCGCATCGCGATCTATCTGACGTACAGCTTCATCGTGCTGATCGTCCTTCTGACGGTGGTGAGACG
ATGGTGA

Upstream 100 bases:

>100_bases
AGGCGCGCGGCCTTTCGCCGCTTGCACGGCGCGCGCCGCTTCGCCATACTCGTTCGAACGTCCGTTGGCGCGACGCTAGG
CGAACCGCGAGTCAAACAAC

Downstream 100 bases:

>100_bases
GCGCATCCGGCATCCTGTCGCAGACGCTCGAGATACTCGTCGCGCTCGCGGCCGCGCCGCTGTTGACCGGCTGGGTCAAC
CAGTGCCGCGCGTGGCTGCA

Product: hydrogenase 4 subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MEQGLLLRCVLFVALAWLALGCAGLANLRRTGVIAHGLFPLGALAGLVLAALGLAGVFAGPSSAVLPLGLPDLPFHLRVD
PLSAYFLFVLGVVTAGVGAFSSGYFRKGEGTAPGLICFEYHVCVASMVLLLVADDAYCFMVAWETLTLSATFLVMTNHRI
AEIRRAAFVYFLISHVGALALLLCFGLLQAGTGDYTFANMRIQHLDGLAASAAFVLALVGFGAKAGIFPLHVWLPDAHPA
APSPVSALLSGFVLKVGLYGILRTVLDLLHVQVAWWGVLMLALGLFGALLGAVFSTIQTDMKRLLAYSSIDNVGLMCVSL
GLTILFLGYRLPSLAALSLTALLYQIVAHACFKSLLFLGTGAVLHATGERNLGRLGGLIRFMPWTAWAMLVGVAASAGLP
PSSGFVSEWLLVQSFLFTPGLPDSVLGMTVPLVAALVALTAALAGYAMVKFFGIVFLGRPREAKLERARDASPWERIAFG
WFAAASVLLGLAPVQYVKVLDALTRALVGAGIAGTNHGWLLFAPVSTARASYMPALFMLFFVACCALAWLLVRRFYHGRL
RRAAPWSCGNPFVTARMQDTAEGFGQPIREIFTPFFRIERQLPSPFDRQPAYRMSVADRTWHLLYEPIAALANRVAALVG
RLQTGRIAIYLTYSFIVLIVLLTVVRRW

Sequences:

>Translated_668_residues
MEQGLLLRCVLFVALAWLALGCAGLANLRRTGVIAHGLFPLGALAGLVLAALGLAGVFAGPSSAVLPLGLPDLPFHLRVD
PLSAYFLFVLGVVTAGVGAFSSGYFRKGEGTAPGLICFEYHVCVASMVLLLVADDAYCFMVAWETLTLSATFLVMTNHRI
AEIRRAAFVYFLISHVGALALLLCFGLLQAGTGDYTFANMRIQHLDGLAASAAFVLALVGFGAKAGIFPLHVWLPDAHPA
APSPVSALLSGFVLKVGLYGILRTVLDLLHVQVAWWGVLMLALGLFGALLGAVFSTIQTDMKRLLAYSSIDNVGLMCVSL
GLTILFLGYRLPSLAALSLTALLYQIVAHACFKSLLFLGTGAVLHATGERNLGRLGGLIRFMPWTAWAMLVGVAASAGLP
PSSGFVSEWLLVQSFLFTPGLPDSVLGMTVPLVAALVALTAALAGYAMVKFFGIVFLGRPREAKLERARDASPWERIAFG
WFAAASVLLGLAPVQYVKVLDALTRALVGAGIAGTNHGWLLFAPVSTARASYMPALFMLFFVACCALAWLLVRRFYHGRL
RRAAPWSCGNPFVTARMQDTAEGFGQPIREIFTPFFRIERQLPSPFDRQPAYRMSVADRTWHLLYEPIAALANRVAALVG
RLQTGRIAIYLTYSFIVLIVLLTVVRRW
>Mature_668_residues
MEQGLLLRCVLFVALAWLALGCAGLANLRRTGVIAHGLFPLGALAGLVLAALGLAGVFAGPSSAVLPLGLPDLPFHLRVD
PLSAYFLFVLGVVTAGVGAFSSGYFRKGEGTAPGLICFEYHVCVASMVLLLVADDAYCFMVAWETLTLSATFLVMTNHRI
AEIRRAAFVYFLISHVGALALLLCFGLLQAGTGDYTFANMRIQHLDGLAASAAFVLALVGFGAKAGIFPLHVWLPDAHPA
APSPVSALLSGFVLKVGLYGILRTVLDLLHVQVAWWGVLMLALGLFGALLGAVFSTIQTDMKRLLAYSSIDNVGLMCVSL
GLTILFLGYRLPSLAALSLTALLYQIVAHACFKSLLFLGTGAVLHATGERNLGRLGGLIRFMPWTAWAMLVGVAASAGLP
PSSGFVSEWLLVQSFLFTPGLPDSVLGMTVPLVAALVALTAALAGYAMVKFFGIVFLGRPREAKLERARDASPWERIAFG
WFAAASVLLGLAPVQYVKVLDALTRALVGAGIAGTNHGWLLFAPVSTARASYMPALFMLFFVACCALAWLLVRRFYHGRL
RRAAPWSCGNPFVTARMQDTAEGFGQPIREIFTPFFRIERQLPSPFDRQPAYRMSVADRTWHLLYEPIAALANRVAALVG
RLQTGRIAIYLTYSFIVLIVLLTVVRRW

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=408, Percent_Identity=24.0196078431373, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI251831116, Length=289, Percent_Identity=28.719723183391, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1788827, Length=618, Percent_Identity=34.789644012945, Blast_Score=315, Evalue=7e-87,
Organism=Escherichia coli, GI2367154, Length=544, Percent_Identity=31.6176470588235, Blast_Score=219, Evalue=6e-58,
Organism=Escherichia coli, GI1788831, Length=356, Percent_Identity=35.1123595505618, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788829, Length=405, Percent_Identity=31.1111111111111, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788614, Length=391, Percent_Identity=32.4808184143223, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788613, Length=428, Percent_Identity=27.1028037383178, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI145693160, Length=219, Percent_Identity=28.7671232876712, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 71851; Mature: 71851

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQGLLLRCVLFVALAWLALGCAGLANLRRTGVIAHGLFPLGALAGLVLAALGLAGVFAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSSAVLPLGLPDLPFHLRVDPLSAYFLFVLGVVTAGVGAFSSGYFRKGEGTAPGLICFEY
CCCCEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHH
HVCVASMVLLLVADDAYCFMVAWETLTLSATFLVMTNHRIAEIRRAAFVYFLISHVGALA
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
LLLCFGLLQAGTGDYTFANMRIQHLDGLAASAAFVLALVGFGAKAGIFPLHVWLPDAHPA
HHHHHHHHHCCCCCEEEHHEEHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
APSPVSALLSGFVLKVGLYGILRTVLDLLHVQVAWWGVLMLALGLFGALLGAVFSTIQTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKRLLAYSSIDNVGLMCVSLGLTILFLGYRLPSLAALSLTALLYQIVAHACFKSLLFLGT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAVLHATGERNLGRLGGLIRFMPWTAWAMLVGVAASAGLPPSSGFVSEWLLVQSFLFTPG
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
LPDSVLGMTVPLVAALVALTAALAGYAMVKFFGIVFLGRPREAKLERARDASPWERIAFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHH
WFAAASVLLGLAPVQYVKVLDALTRALVGAGIAGTNHGWLLFAPVSTARASYMPALFMLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
FVACCALAWLLVRRFYHGRLRRAAPWSCGNPFVTARMQDTAEGFGQPIREIFTPFFRIER
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHH
QLPSPFDRQPAYRMSVADRTWHLLYEPIAALANRVAALVGRLQTGRIAIYLTYSFIVLIV
HCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
LLTVVRRW
HHHHHHCC
>Mature Secondary Structure
MEQGLLLRCVLFVALAWLALGCAGLANLRRTGVIAHGLFPLGALAGLVLAALGLAGVFAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSSAVLPLGLPDLPFHLRVDPLSAYFLFVLGVVTAGVGAFSSGYFRKGEGTAPGLICFEY
CCCCEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHH
HVCVASMVLLLVADDAYCFMVAWETLTLSATFLVMTNHRIAEIRRAAFVYFLISHVGALA
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
LLLCFGLLQAGTGDYTFANMRIQHLDGLAASAAFVLALVGFGAKAGIFPLHVWLPDAHPA
HHHHHHHHHCCCCCEEEHHEEHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
APSPVSALLSGFVLKVGLYGILRTVLDLLHVQVAWWGVLMLALGLFGALLGAVFSTIQTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKRLLAYSSIDNVGLMCVSLGLTILFLGYRLPSLAALSLTALLYQIVAHACFKSLLFLGT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAVLHATGERNLGRLGGLIRFMPWTAWAMLVGVAASAGLPPSSGFVSEWLLVQSFLFTPG
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
LPDSVLGMTVPLVAALVALTAALAGYAMVKFFGIVFLGRPREAKLERARDASPWERIAFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHH
WFAAASVLLGLAPVQYVKVLDALTRALVGAGIAGTNHGWLLFAPVSTARASYMPALFMLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
FVACCALAWLLVRRFYHGRLRRAAPWSCGNPFVTARMQDTAEGFGQPIREIFTPFFRIER
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHH
QLPSPFDRQPAYRMSVADRTWHLLYEPIAALANRVAALVGRLQTGRIAIYLTYSFIVLIV
HCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
LLTVVRRW
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]