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The map label for this gene is potF

Identifier: 53721504

GI number: 53721504

Start: 636535

End: 637656

Strand: Reverse

Name: potF

Synonym: BPSS0467

Alternate gene names: 53721504

Gene position: 637656-636535 (Counterclockwise)

Preceding gene: 53721505

Following gene: 53721503

Centisome position: 20.1

GC content: 64.35

Gene sequence:

>1122_bases
ATGAGTGTGAGCCATCTTCGTCATGCGGTCGCGCGCGCAGCGCTCGTCGCGCTCGCCGGCGCGTCGGCGCTCGCCCTGCC
GGCCGCGCACGCGGCCGGCGCCGAGCTGAACGTGTACAACTGGTCGGACTACATCGCGAAGGACACGATCCCGAACTTCG
AGAAGCAATCCGGCATCCACGTCAAGTACGACAACTACGACAGCGACGACACGCTGCAGGCGAAGCTGCTCGCGGGCAGC
TCCGGCTACGACATCGTCGTGCCGACGTCGAACTACATGGCCAAGCAGATTCAGGCGGGCGTCTACCAGAAGCTCGACAA
ATCGAAGCTGCCGAACCTGTCGAACCTCGATCCGACGCTGATGAAGATGATCGCCGACGCCGATCCGGGCAACCAGTACG
GCGTGCCGTGGGCGTTCGGCACCGACGGCCTCGGCTACAACGTGCAGGCGGTGAAGAAGGCGCTCGGCGAGAACGCGCCC
GTCGACAGCTGGGCGCTCGTGCTCGATCCGGCGAACGTATCCAAGCTCAAGGGCTGCGGCGTATCGTTCCTCGATCAGGC
GGTCGACGTGTTCGCCGCGACGCTACAATACATGGGCAGGAATCCGAACAGCACGAACCCGGCCGATTACCAGGCGGCGT
TCGAGGTGCTGAAGAAAGTCCGCCCGTACATCACGCAATTCAACTCGTCCGGCTACATCAACGATCTCGCGAACAACGAC
GTATGCGTCGCGCTCGGCTGGTCGGGCGACGTCGGCATCGCGCATCGCCGCTCGGCCGAGGCGAAGCGCCCGTACGACAT
CAAGTTCTCGAATCCGAAGGAAGGCGGCCTGCTGTGGTTCGACGTGATGGTGATCCCGAAGGATGCGCCGCACGCGGAAG
CCGCGCTGAAGTGGATCAACTACATCGAGGACCCGAAGGTCAACGCGGCGATCACCAACGAAGTGTTCTACCCGAGCGCG
AACCGCGCGGCGCGACAGTTCGTCGTGCCCGCCGTCGCGCAGGACCCGACCGTCTACCCGCCCGAGGACGTGCTCAAGAA
GATGACGCTGATGCGGCCGATGCCCGCCGACATCCTGCGGCTCGAGAACCGTCTGTGGGCGCAATTGAAGACGGGACACT
GA

Upstream 100 bases:

>100_bases
GATGCGACGGCGCAGCAACTGGGTCTTTCCTAACGGCATTGCCAATCGGGCAGGCGGCGCGCGGGCGCCGCGCGCCCACC
TTCATCATCAGGGGAACAAC

Downstream 100 bases:

>100_bases
TCACGGGCGATTCCGGCGCGCGGCGATCGCCCGATTGGCCGCCGCGCGCCCCGAGCAATCCGGCGCCGGCGCGTTCGACC
TGTCACGCGCGGCGCCTCGA

Product: putrescine ABC transport system, binding exported protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSVSHLRHAVARAALVALAGASALALPAAHAAGAELNVYNWSDYIAKDTIPNFEKQSGIHVKYDNYDSDDTLQAKLLAGS
SGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTLMKMIADADPGNQYGVPWAFGTDGLGYNVQAVKKALGENAP
VDSWALVLDPANVSKLKGCGVSFLDQAVDVFAATLQYMGRNPNSTNPADYQAAFEVLKKVRPYITQFNSSGYINDLANND
VCVALGWSGDVGIAHRRSAEAKRPYDIKFSNPKEGGLLWFDVMVIPKDAPHAEAALKWINYIEDPKVNAAITNEVFYPSA
NRAARQFVVPAVAQDPTVYPPEDVLKKMTLMRPMPADILRLENRLWAQLKTGH

Sequences:

>Translated_373_residues
MSVSHLRHAVARAALVALAGASALALPAAHAAGAELNVYNWSDYIAKDTIPNFEKQSGIHVKYDNYDSDDTLQAKLLAGS
SGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTLMKMIADADPGNQYGVPWAFGTDGLGYNVQAVKKALGENAP
VDSWALVLDPANVSKLKGCGVSFLDQAVDVFAATLQYMGRNPNSTNPADYQAAFEVLKKVRPYITQFNSSGYINDLANND
VCVALGWSGDVGIAHRRSAEAKRPYDIKFSNPKEGGLLWFDVMVIPKDAPHAEAALKWINYIEDPKVNAAITNEVFYPSA
NRAARQFVVPAVAQDPTVYPPEDVLKKMTLMRPMPADILRLENRLWAQLKTGH
>Mature_372_residues
SVSHLRHAVARAALVALAGASALALPAAHAAGAELNVYNWSDYIAKDTIPNFEKQSGIHVKYDNYDSDDTLQAKLLAGSS
GYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTLMKMIADADPGNQYGVPWAFGTDGLGYNVQAVKKALGENAPV
DSWALVLDPANVSKLKGCGVSFLDQAVDVFAATLQYMGRNPNSTNPADYQAAFEVLKKVRPYITQFNSSGYINDLANNDV
CVALGWSGDVGIAHRRSAEAKRPYDIKFSNPKEGGLLWFDVMVIPKDAPHAEAALKWINYIEDPKVNAAITNEVFYPSAN
RAARQFVVPAVAQDPTVYPPEDVLKKMTLMRPMPADILRLENRLWAQLKTGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=367, Percent_Identity=47.9564032697548, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1787367, Length=323, Percent_Identity=36.5325077399381, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1787711, Length=333, Percent_Identity=25.2252252252252, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40683; Mature: 40551

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSHLRHAVARAALVALAGASALALPAAHAAGAELNVYNWSDYIAKDTIPNFEKQSGIH
CCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCEEEEECCHHHHHHCCCCCCHHCCCCE
VKYDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTL
EEECCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
MKMIADADPGNQYGVPWAFGTDGLGYNVQAVKKALGENAPVDSWALVLDPANVSKLKGCG
HHHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHCCCC
VSFLDQAVDVFAATLQYMGRNPNSTNPADYQAAFEVLKKVRPYITQFNSSGYINDLANND
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCC
VCVALGWSGDVGIAHRRSAEAKRPYDIKFSNPKEGGLLWFDVMVIPKDAPHAEAALKWIN
EEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
YIEDPKVNAAITNEVFYPSANRAARQFVVPAVAQDPTVYPPEDVLKKMTLMRPMPADILR
HHCCCCCCEEEECCEECCCCCHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHH
LENRLWAQLKTGH
HHHHHHHHCCCCC
>Mature Secondary Structure 
SVSHLRHAVARAALVALAGASALALPAAHAAGAELNVYNWSDYIAKDTIPNFEKQSGIH
CHHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCEEEEECCHHHHHHCCCCCCHHCCCCE
VKYDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTL
EEECCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
MKMIADADPGNQYGVPWAFGTDGLGYNVQAVKKALGENAPVDSWALVLDPANVSKLKGCG
HHHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHCCCC
VSFLDQAVDVFAATLQYMGRNPNSTNPADYQAAFEVLKKVRPYITQFNSSGYINDLANND
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCC
VCVALGWSGDVGIAHRRSAEAKRPYDIKFSNPKEGGLLWFDVMVIPKDAPHAEAALKWIN
EEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
YIEDPKVNAAITNEVFYPSANRAARQFVVPAVAQDPTVYPPEDVLKKMTLMRPMPADILR
HHCCCCCCEEEECCEECCCCCHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHH
LENRLWAQLKTGH
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]