Click here to switch to the map view.

The map label for this gene is dmlR [H]

Identifier: 53719799

GI number: 53719799

Start: 2628947

End: 2629849

Strand: Reverse

Name: dmlR [H]

Synonym: BPSL2190

Alternate gene names: 53719799

Gene position: 2629849-2628947 (Counterclockwise)

Preceding gene: 53719802

Following gene: 53719798

Centisome position: 64.54

GC content: 69.99

Gene sequence:

>903_bases
ATGGATCGCTTCAAACAGATCGAAACGTTCGCCGCGGTTGCGGCGAAAGGCAGCCTGTCGGCGGCCGCGCATGCGGAAGG
GGTCGCGCCCGCGATCATCGGCCGCCGGCTTGACGCGCTCGAGGAGCGGCTCGGCGTGAAGCTGCTCGTGCGCACCACGC
GCAAGCTCACGCTGACGTTCGAAGGCTCGGCGTTCCTCGAGGATTGCCAGCGCATCATCAACGACATGCAGAACGCCGAG
GCGAGCGTGTCCGCGGGCGGCGTGAAGGCGAGCGGGCATTTGCGGGTGTCGGCGCCCGCGGGCTTCGGCCGGCGCCACGT
CGCGCCGCTCGTACCCCCGTTTACGGGCGCGCACCCGGACGTGTCGGTCACGCTCGATCTGTCCGACCGGATGGTCGACC
TCGTCAACGAGGGGTTCGATTGCGCGGTGCGCCTGGGCGAGCTGCCCGATTCGTCGCTCGTGTCGCTCAAGCTCGGCGAG
AACCGGCGCGTGTGCGTCGCGTCGCCGGCCTATCTCGCGCGCGCCGGCATGCCCGGGACGCTCGACGATCTCGCGCGCCA
CAACTGCCTCGCGCTCGCGGCGAACGCGAACCAGCAGCGCGGCTGGAGCTTCGTCGACGGCGGCAAGGTGGTGTCGATCC
GCGTGTCCGGCACGATGGAGTGCTCGGACGGCGCGGTGCTGCACGAATGGTGCCTCGAGGGCCATGGCCTCGCATGGCGC
TCGTGGTGGGAAGTCGGCGCGGACATCGCGGCGGGCCGGCTCGTCAGCGTGCTCGACGCGTTCGCCGCGCCGCCGGTCGG
CATTCACGCGGTGTTTCCGCAGCGCCGGCACTTGCCGCTCAGGGTCCGGCTCTTTCTCGATTTCCTGAAGCACACGTACG
AACGCCCCGGCTATTGGGGATGA

Upstream 100 bases:

>100_bases
AAAAGACAGTAATCATCACTTGATCTTTTACTTTTCGATATCTAATCTGGAGCGATTTCGGATTCGACGAGGCGGCCGGT
GCGCGCCGCCCGCATACGGG

Downstream 100 bases:

>100_bases
CGTTCGCGCGACGGCGCGTGCCATGGCCGCAGGCGCGGCGTTTCCGCGCGCCGCTCCGGCTCGCGTCGTGCGCGCCGCCG
AATTTGCGCTCCAATGCGTA

Product: LysR family regulatory protein

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAE
ASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPPFTGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGE
NRRVCVASPAYLARAGMPGTLDDLARHNCLALAANANQQRGWSFVDGGKVVSIRVSGTMECSDGAVLHEWCLEGHGLAWR
SWWEVGADIAAGRLVSVLDAFAAPPVGIHAVFPQRRHLPLRVRLFLDFLKHTYERPGYWG

Sequences:

>Translated_300_residues
MDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAE
ASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPPFTGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGE
NRRVCVASPAYLARAGMPGTLDDLARHNCLALAANANQQRGWSFVDGGKVVSIRVSGTMECSDGAVLHEWCLEGHGLAWR
SWWEVGADIAAGRLVSVLDAFAAPPVGIHAVFPQRRHLPLRVRLFLDFLKHTYERPGYWG
>Mature_300_residues
MDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAE
ASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPPFTGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGE
NRRVCVASPAYLARAGMPGTLDDLARHNCLALAANANQQRGWSFVDGGKVVSIRVSGTMECSDGAVLHEWCLEGHGLAWR
SWWEVGADIAAGRLVSVLDAFAAPPVGIHAVFPQRRHLPLRVRLFLDFLKHTYERPGYWG

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=35.5172413793103, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=31.4685314685315, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=33.1081081081081, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=30.6896551724138, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=30.0353356890459, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=29.616724738676, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=28.4280936454849, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789173, Length=250, Percent_Identity=28.4, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790011, Length=297, Percent_Identity=24.5791245791246, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786448, Length=279, Percent_Identity=26.1648745519713, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788706, Length=189, Percent_Identity=26.984126984127, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32351; Mature: 32351

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTF
CCHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEECEEEEEEE
EGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPPFTGAHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCC
VSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARAGMPGT
EEEEEECHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHHCCCCCC
LDDLARHNCLALAANANQQRGWSFVDGGKVVSIRVSGTMECSDGAVLHEWCLEGHGLAWR
HHHHHCCCEEEEEECCCCCCCCEEECCCEEEEEEEECEEECCCCCHHHHHHHCCCCCCHH
SWWEVGADIAAGRLVSVLDAFAAPPVGIHAVFPQRRHLPLRVRLFLDFLKHTYERPGYWG
HHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTF
CCHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEECEEEEEEE
EGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPPFTGAHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCC
VSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARAGMPGT
EEEEEECHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHHCCCCCC
LDDLARHNCLALAANANQQRGWSFVDGGKVVSIRVSGTMECSDGAVLHEWCLEGHGLAWR
HHHHHCCCEEEEEECCCCCCCCEEECCCEEEEEEEECEEECCCCCHHHHHHHCCCCCCHH
SWWEVGADIAAGRLVSVLDAFAAPPVGIHAVFPQRRHLPLRVRLFLDFLKHTYERPGYWG
HHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]