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The map label for this gene is citA [H]

Identifier: 53719301

GI number: 53719301

Start: 1956681

End: 1957964

Strand: Reverse

Name: citA [H]

Synonym: BPSL1677

Alternate gene names: 53719301

Gene position: 1957964-1956681 (Counterclockwise)

Preceding gene: 53719302

Following gene: 53719300

Centisome position: 48.05

GC content: 69.63

Gene sequence:

>1284_bases
GTGTCGCCCCCTACGTCGAGCGCCGGGCGAGCAATCGCCGCGGCGTCGATCGGCAACGCGCTCGAGTGGTACGACTTTTC
CGTCTATGCATTCTTCGCCGTCTACATCGCGCGGAACTTCTTCCATCGAGGCAACACCGGCACCGAGCTGGTCGAGGCTT
TCATGGCGTTCGGCATCGGCTTCATCGCACGGCCGCTCGGCGCGCTCGCGATCGGTGTATACGGCGACCGCGCGGGCCGC
AAGGCCGCGCTCACGCTGACCATCCTCGTGATGGCAACCGGCACGGGCGTCATCGCGTTCGCGCCGCCATACGCCGCGAT
CGGCGTGGGCGCGCCGCTGCTGATCCTCTGCGGGAGGCTACTGCAGGGCTTCTCGGCGGGCGGCGAAGTGGGCGGCGCGG
CGGCGTTTCTCATCGAGCACGCGCCGGCGGACCGCAAGGGCTGCTACGCGTCGTGTCTGCAGGCGAGCATGGCCGCGTCG
AACATCCTCGGCGCGCTGGTCGCGACCGGCGTGACGCTTACGCTGACGCGCGAACAGATCGGCGATTGGGGATGGCGGAT
TCCGTTCATCCTCGGCCTCGCGATCGCGCCGGTCGGCCTCTGGCTGCGCAGGACGCTCGACGAGACGCCGCACTTCCGCG
CCGAGATGGCGCGCGCGCAGCACGCGCATGCGGAACAGAAAGCGCCGCTTCTGCAGGTGGTGCGCGACCACCCGCGCGCG
CTCGCCGTCGGCACGGGATTCTCGGTGCTCTGGGCCGTGTGCGTCTACGCGCTGGTGATCTATATGCCGACGCACGCGCA
GCGCGCACTGCATTTCGACGGGCGCGACGCGTTCATCGCGTCGCTGGTCGGCAACTGCCTGATGGCCGTCACCTGCGTGT
GCGCGGGAAGCTGGTCCGACCGCCTCGGCCGGCGCACGGTGCTCGCCGCCGGCGCGGCGCTGATGCTCGTGTCGGTCTAT
CCGCTGCTGCGCTGGCTGAGCGACGTGCACACGCTCGCCGCGCTCCTTACCGTCCAGAGCGCGTTCTGCGTGTTGGTGGC
CATCTTCACGGGAGTGGCGCCCGCAGCGCTGTCGGAGCTGTTCCCGACCCGCGTACGCGCGACCGGCATGTCCCTGTCCT
ACAACATCGCCACGACGATCTTCGGCGGCTTCGCGCCCGCGATCCTCGCATGGCTCACGCAACAGACCGGCAGTCCGTTT
GCGCCGGCCTGGTACGTGATGGTGGCGAGCGCCATCGCGCTCGCATCGATCGCCGCGCTTTCTTCCACGCCACGCCACGC
CTGA

Upstream 100 bases:

>100_bases
GCAAATTCCGCATTTCCGGCTGTAAAGGCCGCCCCGCACGACGACAGGACAGGAGATCGAAGTGAAGCAATCCGCAGTTC
CCCTCGACACGGGCACGGCC

Downstream 100 bases:

>100_bases
TTTCGGAGAACCGCTCCATGCAACGCTTCCTCACGCGGAGCGCGATCGCGCTCGCACTTTTCGCCGCCGTCCCGGCCTTC
GCGGACGTCACGCTCGACGG

Product: putative transport-related, integral membrane protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSPPTSSAGRAIAAASIGNALEWYDFSVYAFFAVYIARNFFHRGNTGTELVEAFMAFGIGFIARPLGALAIGVYGDRAGR
KAALTLTILVMATGTGVIAFAPPYAAIGVGAPLLILCGRLLQGFSAGGEVGGAAAFLIEHAPADRKGCYASCLQASMAAS
NILGALVATGVTLTLTREQIGDWGWRIPFILGLAIAPVGLWLRRTLDETPHFRAEMARAQHAHAEQKAPLLQVVRDHPRA
LAVGTGFSVLWAVCVYALVIYMPTHAQRALHFDGRDAFIASLVGNCLMAVTCVCAGSWSDRLGRRTVLAAGAALMLVSVY
PLLRWLSDVHTLAALLTVQSAFCVLVAIFTGVAPAALSELFPTRVRATGMSLSYNIATTIFGGFAPAILAWLTQQTGSPF
APAWYVMVASAIALASIAALSSTPRHA

Sequences:

>Translated_427_residues
MSPPTSSAGRAIAAASIGNALEWYDFSVYAFFAVYIARNFFHRGNTGTELVEAFMAFGIGFIARPLGALAIGVYGDRAGR
KAALTLTILVMATGTGVIAFAPPYAAIGVGAPLLILCGRLLQGFSAGGEVGGAAAFLIEHAPADRKGCYASCLQASMAAS
NILGALVATGVTLTLTREQIGDWGWRIPFILGLAIAPVGLWLRRTLDETPHFRAEMARAQHAHAEQKAPLLQVVRDHPRA
LAVGTGFSVLWAVCVYALVIYMPTHAQRALHFDGRDAFIASLVGNCLMAVTCVCAGSWSDRLGRRTVLAAGAALMLVSVY
PLLRWLSDVHTLAALLTVQSAFCVLVAIFTGVAPAALSELFPTRVRATGMSLSYNIATTIFGGFAPAILAWLTQQTGSPF
APAWYVMVASAIALASIAALSSTPRHA
>Mature_426_residues
SPPTSSAGRAIAAASIGNALEWYDFSVYAFFAVYIARNFFHRGNTGTELVEAFMAFGIGFIARPLGALAIGVYGDRAGRK
AALTLTILVMATGTGVIAFAPPYAAIGVGAPLLILCGRLLQGFSAGGEVGGAAAFLIEHAPADRKGCYASCLQASMAASN
ILGALVATGVTLTLTREQIGDWGWRIPFILGLAIAPVGLWLRRTLDETPHFRAEMARAQHAHAEQKAPLLQVVRDHPRAL
AVGTGFSVLWAVCVYALVIYMPTHAQRALHFDGRDAFIASLVGNCLMAVTCVCAGSWSDRLGRRTVLAAGAALMLVSVYP
LLRWLSDVHTLAALLTVQSAFCVLVAIFTGVAPAALSELFPTRVRATGMSLSYNIATTIFGGFAPAILAWLTQQTGSPFA
PAWYVMVASAIALASIAALSSTPRHA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=422, Percent_Identity=32.7014218009479, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788942, Length=420, Percent_Identity=35.4761904761905, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1788292, Length=428, Percent_Identity=29.9065420560748, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1789941, Length=407, Percent_Identity=33.4152334152334, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87082231, Length=438, Percent_Identity=24.4292237442922, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082404, Length=403, Percent_Identity=22.0843672456576, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44897; Mature: 44766

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPTSSAGRAIAAASIGNALEWYDFSVYAFFAVYIARNFFHRGNTGTELVEAFMAFGIG
CCCCCCCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FIARPLGALAIGVYGDRAGRKAALTLTILVMATGTGVIAFAPPYAAIGVGAPLLILCGRL
HHHHHHHHHEEEECCCCCCCCHHHHEEEEEEECCCCEEEECCCHHHHHCCCHHHHHHHHH
LQGFSAGGEVGGAAAFLIEHAPADRKGCYASCLQASMAASNILGALVATGVTLTLTREQI
HHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHH
GDWGWRIPFILGLAIAPVGLWLRRTLDETPHFRAEMARAQHAHAEQKAPLLQVVRDHPRA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCE
LAVGTGFSVLWAVCVYALVIYMPTHAQRALHFDGRDAFIASLVGNCLMAVTCVCAGSWSD
EEECCCHHHHHHHHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCHHH
RLGRRTVLAAGAALMLVSVYPLLRWLSDVHTLAALLTVQSAFCVLVAIFTGVAPAALSEL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FPTRVRATGMSLSYNIATTIFGGFAPAILAWLTQQTGSPFAPAWYVMVASAIALASIAAL
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
SSTPRHA
CCCCCCC
>Mature Secondary Structure 
SPPTSSAGRAIAAASIGNALEWYDFSVYAFFAVYIARNFFHRGNTGTELVEAFMAFGIG
CCCCCCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FIARPLGALAIGVYGDRAGRKAALTLTILVMATGTGVIAFAPPYAAIGVGAPLLILCGRL
HHHHHHHHHEEEECCCCCCCCHHHHEEEEEEECCCCEEEECCCHHHHHCCCHHHHHHHHH
LQGFSAGGEVGGAAAFLIEHAPADRKGCYASCLQASMAASNILGALVATGVTLTLTREQI
HHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHH
GDWGWRIPFILGLAIAPVGLWLRRTLDETPHFRAEMARAQHAHAEQKAPLLQVVRDHPRA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCE
LAVGTGFSVLWAVCVYALVIYMPTHAQRALHFDGRDAFIASLVGNCLMAVTCVCAGSWSD
EEECCCHHHHHHHHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCHHH
RLGRRTVLAAGAALMLVSVYPLLRWLSDVHTLAALLTVQSAFCVLVAIFTGVAPAALSEL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FPTRVRATGMSLSYNIATTIFGGFAPAILAWLTQQTGSPFAPAWYVMVASAIALASIAAL
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
SSTPRHA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]