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The map label for this gene is rluE [H]

Identifier: 53718535

GI number: 53718535

Start: 1043345

End: 1043923

Strand: Reverse

Name: rluE [H]

Synonym: BPSL0895

Alternate gene names: 53718535

Gene position: 1043923-1043345 (Counterclockwise)

Preceding gene: 53718538

Following gene: 53718534

Centisome position: 25.62

GC content: 73.23

Gene sequence:

>579_bases
ATGCGCCTCATCGCCCTCAACAAGCCGTTCGGCACGATCTGCCAGTTCTCTGCGCACGAAACCCGCGCATCGCTCGGCGA
CTGGGTGAAGACGCCCGGCGTCTATCCGGCGGGCCGCCTCGATGCCGACAGCGAGGGGCTGCTCCTGCTCACCGACGACG
GCGCGCTGCAGGCGAAGATCGCCGAGCCGCGCCACAAGCTCGTCAAGCGCTACTGGGCGCAGGTCGAGGGCGCGCCCGAC
GCGGCCGCGCTCGCCGCGCTCGAGCGCGGCGTCGATCTCGGCGATTACGTGAGCCGCCCGTGTCGCGCGCGCTTCATCAC
GCCGCCCGGCGCGCTCTGGCCGCGCAACCCGCCGATCCGCCACCGCGCGGCGATCCCGACGGCCTGGATCGAGCTCGAGA
TCGCCGAAGGCAAGAACCGCCAGGTGCGGCGAATGACCGCGGCCGTCGGTTTCCCGACGCTGCGCCTCGTTCGCGTCGGC
ATCGGCGCGCTCGACGTGTTCGCGCTCGGCCTTGCGCCGGGCGAAACGCGCGAGCTGCCGCCGCGCGCGCCGTGGGAACG
CGCACAGCGGCCCGGATGA

Upstream 100 bases:

>100_bases
ACAAAAGACTCGCCGTTCCGTATTATGCATTAAGATTCCGCCATTTGCCATAGAACGCGGCCCGCGTCGCGCCGCCCCTC
TCCCCCGCGAGCCTTGCCCC

Downstream 100 bases:

>100_bases
CAATTCGGTGACGCGTTTCGCCCCGCGTCAACCGACTGCGAACCCGACGCGTTAAACCACGCAGATGCCACTCCCAGCTA
TCCGGCATTGGCGACCAAAC

Product: putative pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MRLIALNKPFGTICQFSAHETRASLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQAKIAEPRHKLVKRYWAQVEGAPD
AAALAALERGVDLGDYVSRPCRARFITPPGALWPRNPPIRHRAAIPTAWIELEIAEGKNRQVRRMTAAVGFPTLRLVRVG
IGALDVFALGLAPGETRELPPRAPWERAQRPG

Sequences:

>Translated_192_residues
MRLIALNKPFGTICQFSAHETRASLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQAKIAEPRHKLVKRYWAQVEGAPD
AAALAALERGVDLGDYVSRPCRARFITPPGALWPRNPPIRHRAAIPTAWIELEIAEGKNRQVRRMTAAVGFPTLRLVRVG
IGALDVFALGLAPGETRELPPRAPWERAQRPG
>Mature_192_residues
MRLIALNKPFGTICQFSAHETRASLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQAKIAEPRHKLVKRYWAQVEGAPD
AAALAALERGVDLGDYVSRPCRARFITPPGALWPRNPPIRHRAAIPTAWIELEIAEGKNRQVRRMTAAVGFPTLRLVRVG
IGALDVFALGLAPGETRELPPRAPWERAQRPG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=51.123595505618, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1788510, Length=182, Percent_Identity=33.5164835164835, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787524, Length=166, Percent_Identity=32.5301204819277, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790453, Length=178, Percent_Identity=29.2134831460674, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20995; Mature: 20995

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIALNKPFGTICQFSAHETRASLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQAKI
CEEEEECCCCHHHEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEC
AEPRHKLVKRYWAQVEGAPDAAALAALERGVDLGDYVSRPCRARFITPPGALWPRNPPIR
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCEEEECCCCCCCCCCCCCC
HRAAIPTAWIELEIAEGKNRQVRRMTAAVGFPTLRLVRVGIGALDVFALGLAPGETRELP
CCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCCCCCC
PRAPWERAQRPG
CCCCHHHCCCCC
>Mature Secondary Structure
MRLIALNKPFGTICQFSAHETRASLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQAKI
CEEEEECCCCHHHEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEC
AEPRHKLVKRYWAQVEGAPDAAALAALERGVDLGDYVSRPCRARFITPPGALWPRNPPIR
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCEEEECCCCCCCCCCCCCC
HRAAIPTAWIELEIAEGKNRQVRRMTAAVGFPTLRLVRVGIGALDVFALGLAPGETRELP
CCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCCCCCC
PRAPWERAQRPG
CCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]