Click here to switch to the map view.

The map label for this gene is rpfG [H]

Identifier: 53718347

GI number: 53718347

Start: 803697

End: 805016

Strand: Reverse

Name: rpfG [H]

Synonym: BPSL0704

Alternate gene names: 53718347

Gene position: 805016-803697 (Counterclockwise)

Preceding gene: 53718349

Following gene: 53718346

Centisome position: 19.76

GC content: 66.74

Gene sequence:

>1320_bases
GTGATCCTGATCGTCGACGACGAGCCGAGCATCCTGTCCGCGCTCAAGCGCCTGCTGCGCACCGCGCGCTACCAGGTCGT
GACGGCCGAGAGCGGCGCCGCGGCACTCGACGTGCTCGCCGTCGGCGAAGTCGACCTGATCATCTCCGACATGCGGATGC
CCTGCATGACGGGCGCCGAATTCCTCGCGCGCGCGCAGACCCTCCATCCGGACACGATGCGCATCCTGCTCACCGGCTAT
TCCGAGATCGACGCGGTGGTGAGCGCGATCAACGAAGGCGGCGTGTATCGCTATCTGAACAAGCCGTGGGACGATCACGA
CCTGCTGCTCACGGTCAGGCAGGCGCTCGAGCAAAGACGCCTGCGCCAGGAGACCGCGCGCCTGTTCGCGCTCACGCAGA
AGCAGAACGAAGAGCTCGTCGCGTTCAGCACGGAGCTCGAAGCGCAGGTGCACGCGCGCACCGAGGAAATCCGGCAGACC
GTGATGTTTCTCGAAGATGCGCAGCGCGACCTGAAGCGCAATTTCATGACGATGATCCAGGTCGGCGCGAACATGATCGA
GCTGCGCTGCGGCGTGATGGGCGGCGAGTCGCGGCGCATCGGCGAACTCGCGAAGCGCCTCGCGCTCGCGCTCGACATGA
GCGAGCTGCAAGCGCAGGATCTGTATTTCGCCGGCCTCCTGCACGGAATCGGCAAGCTGTCGCTGCCCGACGAGCTGCTG
CGCAAATCGATCGACCGAATGAGCGCCGAAGAAAGCCGGCTGTACCACCAGCATCCGCTGCGCGCGCAGATGGTGCTCAC
GCCCGTCACGCAGCTCAATCACGTCGCGCACATCATTCGTCACCAGTATGAGCGCTTCAACGGGCGCGGCACGCCCGACG
GCCTCGCGGGCGACGACATTCCGCTCGGCTCGCGCATCCTCGCGATCGCGCGCGACTACGAGGGATTGCAGCGCGGCGGC
GTCGTCAATCAGCGCCTGCAGTCCGAGCAGGCGATCGCGCTCATCAAGTCGCAGGCGGGGCTGCGCTACGATCGGCAAGT
CGTCGATCGCTTCGTCGCGCTCGTGAAGGATCTGGCGACGCTCGGCTGCAACACGCCGTATGCGCGGATCACGGGCGGCC
AGTTGCTCGAAGGCATGCGGCTCGCCGACGATCTGCGCACGAGCCGCGGCGTGCTGCTGATGACGAAGGGCAGCGTCGTG
TCCGCGCACCAGGTCGCGCTCGTGCGGCGCTACGAGGCCCAGGAAAGCACGCCGTTCGCGATCCTGATCCAGACGACGAC
GCTAGCCGAAGCCGCGGCGAGCGCCGGGCGCGAATGCTGA

Upstream 100 bases:

>100_bases
GCCCATCGCGCCGGATGCGCCGCAGCCGCATGCGGCCGATCACGCGCGCAGCGCCGGTTCCGCCTGCGCCGGCGCGGACG
GTGGCGCGCCCGCCGCGCCC

Downstream 100 bases:

>100_bases
CGCGCGCGTCGCACCCGCCGCCCGCGCGCATCGGGAAAAACCCACGTCCATGCATGGCACCTTCAATCTGCCGCTCGTCG
CGCTGTCGCTCGCGATCGCA

Product: putative two-component response regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MILIVDDEPSILSALKRLLRTARYQVVTAESGAAALDVLAVGEVDLIISDMRMPCMTGAEFLARAQTLHPDTMRILLTGY
SEIDAVVSAINEGGVYRYLNKPWDDHDLLLTVRQALEQRRLRQETARLFALTQKQNEELVAFSTELEAQVHARTEEIRQT
VMFLEDAQRDLKRNFMTMIQVGANMIELRCGVMGGESRRIGELAKRLALALDMSELQAQDLYFAGLLHGIGKLSLPDELL
RKSIDRMSAEESRLYHQHPLRAQMVLTPVTQLNHVAHIIRHQYERFNGRGTPDGLAGDDIPLGSRILAIARDYEGLQRGG
VVNQRLQSEQAIALIKSQAGLRYDRQVVDRFVALVKDLATLGCNTPYARITGGQLLEGMRLADDLRTSRGVLLMTKGSVV
SAHQVALVRRYEAQESTPFAILIQTTTLAEAAASAGREC

Sequences:

>Translated_439_residues
MILIVDDEPSILSALKRLLRTARYQVVTAESGAAALDVLAVGEVDLIISDMRMPCMTGAEFLARAQTLHPDTMRILLTGY
SEIDAVVSAINEGGVYRYLNKPWDDHDLLLTVRQALEQRRLRQETARLFALTQKQNEELVAFSTELEAQVHARTEEIRQT
VMFLEDAQRDLKRNFMTMIQVGANMIELRCGVMGGESRRIGELAKRLALALDMSELQAQDLYFAGLLHGIGKLSLPDELL
RKSIDRMSAEESRLYHQHPLRAQMVLTPVTQLNHVAHIIRHQYERFNGRGTPDGLAGDDIPLGSRILAIARDYEGLQRGG
VVNQRLQSEQAIALIKSQAGLRYDRQVVDRFVALVKDLATLGCNTPYARITGGQLLEGMRLADDLRTSRGVLLMTKGSVV
SAHQVALVRRYEAQESTPFAILIQTTTLAEAAASAGREC
>Mature_439_residues
MILIVDDEPSILSALKRLLRTARYQVVTAESGAAALDVLAVGEVDLIISDMRMPCMTGAEFLARAQTLHPDTMRILLTGY
SEIDAVVSAINEGGVYRYLNKPWDDHDLLLTVRQALEQRRLRQETARLFALTQKQNEELVAFSTELEAQVHARTEEIRQT
VMFLEDAQRDLKRNFMTMIQVGANMIELRCGVMGGESRRIGELAKRLALALDMSELQAQDLYFAGLLHGIGKLSLPDELL
RKSIDRMSAEESRLYHQHPLRAQMVLTPVTQLNHVAHIIRHQYERFNGRGTPDGLAGDDIPLGSRILAIARDYEGLQRGG
VVNQRLQSEQAIALIKSQAGLRYDRQVVDRFVALVKDLATLGCNTPYARITGGQLLEGMRLADDLRTSRGVLLMTKGSVV
SAHQVALVRRYEAQESTPFAILIQTTTLAEAAASAGREC

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=117, Percent_Identity=42.7350427350427, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790299, Length=124, Percent_Identity=35.4838709677419, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=33.587786259542, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=36.5217391304348, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 48856; Mature: 48856

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIVDDEPSILSALKRLLRTARYQVVTAESGAAALDVLAVGEVDLIISDMRMPCMTGAE
CEEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHEEEECCHHHHHHCCCCCHHHHHH
FLARAQTLHPDTMRILLTGYSEIDAVVSAINEGGVYRYLNKPWDDHDLLLTVRQALEQRR
HHHHHHCCCCCHHEEEEECHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHH
LRQETARLFALTQKQNEELVAFSTELEAQVHARTEEIRQTVMFLEDAQRDLKRNFMTMIQ
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGANMIELRCGVMGGESRRIGELAKRLALALDMSELQAQDLYFAGLLHGIGKLSLPDELL
HCCHHEEHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RKSIDRMSAEESRLYHQHPLRAQMVLTPVTQLNHVAHIIRHQYERFNGRGTPDGLAGDDI
HHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PLGSRILAIARDYEGLQRGGVVNQRLQSEQAIALIKSQAGLRYDRQVVDRFVALVKDLAT
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LGCNTPYARITGGQLLEGMRLADDLRTSRGVLLMTKGSVVSAHQVALVRRYEAQESTPFA
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEE
ILIQTTTLAEAAASAGREC
EEEEEHHHHHHHHHCCCCC
>Mature Secondary Structure
MILIVDDEPSILSALKRLLRTARYQVVTAESGAAALDVLAVGEVDLIISDMRMPCMTGAE
CEEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHEEEECCHHHHHHCCCCCHHHHHH
FLARAQTLHPDTMRILLTGYSEIDAVVSAINEGGVYRYLNKPWDDHDLLLTVRQALEQRR
HHHHHHCCCCCHHEEEEECHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHH
LRQETARLFALTQKQNEELVAFSTELEAQVHARTEEIRQTVMFLEDAQRDLKRNFMTMIQ
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGANMIELRCGVMGGESRRIGELAKRLALALDMSELQAQDLYFAGLLHGIGKLSLPDELL
HCCHHEEHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RKSIDRMSAEESRLYHQHPLRAQMVLTPVTQLNHVAHIIRHQYERFNGRGTPDGLAGDDI
HHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PLGSRILAIARDYEGLQRGGVVNQRLQSEQAIALIKSQAGLRYDRQVVDRFVALVKDLAT
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LGCNTPYARITGGQLLEGMRLADDLRTSRGVLLMTKGSVVSAHQVALVRRYEAQESTPFA
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEE
ILIQTTTLAEAAASAGREC
EEEEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]