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The map label for this gene is prs [H]

Identifier: 53718161

GI number: 53718161

Start: 574124

End: 575080

Strand: Reverse

Name: prs [H]

Synonym: BPSL0521

Alternate gene names: 53718161

Gene position: 575080-574124 (Counterclockwise)

Preceding gene: 53718162

Following gene: 161723210

Centisome position: 14.11

GC content: 63.95

Gene sequence:

>957_bases
ATGAGCAGCCATGACGGCCTGATGGTTTTTACTGGCAACGCCAATCCCGCGCTTGCTCAGGAAGTCGTCAAAATTCTGGG
TATTCCCCTCGGCAAAGCAATGGTCAGCCGTTTCTCCGACGGCGAGATCCAAGTCGAAATCCAGGAAAACGTGCGCGGCA
AGGACGTCTTCGTCCTGCAATCGACGTGCGCGCCGACGAACGACAACCTGATGGAACTGATGATCATGGTCGATGCGCTC
AAGCGCGCATCCGCCGGCCGGATCACCGCCGCCATCCCCTACTTCGGCTATGCGCGCCAGGATCGCCGCCCGCGTTCGGC
GCGCGTCGCGATCTCCGCGAAGGTCGTCGCGAACATGCTGGAAATCGCCGGCGTCGAGCGGATCATCACGATGGATCTGC
ACGCCGACCAGATTCAAGGCTTCTTCGACATCCCCGTCGACAACATCTACGCGACGCCGATCCTGCTGGGCGACCTGCGC
AAGCAGAACTACCCGGATCTGCTCGTCGTGTCGCCGGACGTCGGCGGCGTCGTGCGCGCCCGCGCGCTCGCGAAGCAGCT
GAACTGCGATCTCGCGATCATCGACAAGCGCCGTCCGAAGGCGAATGTCGCCGAAGTGATGAACATCATCGGTGAAGTCG
AAGGCCGCACCTGCGTGATCATGGACGACATGGTCGACACGGCGGGCACGCTCTGCAAGGCCGCGCAAGTGCTCAAGGAG
CGCGGCGCGAAGCAGGTGTTCGCGTATGCGACGCACCCGGTGCTCTCGGGCGGCGCGGCCGACCGGATCGCCGCTTCGGC
GCTCGACGAACTTGTCGTCACCGACACGATTCCGCTGTCCGCCGAATCGCTCGCGTGCCCGAAGATCCGCGCGCTATCGA
GCGCGGGCCTCCTCGCCGAGACGTTCTCGCGGATCCGCCGCGGCGATTCGGTGATGTCGCTGTTCGCGGAATCGTAA

Upstream 100 bases:

>100_bases
GCACCGGATTTTGATTCCGGCATGCGAGGGTTCGAGTCCTTCCTCCCCTGCCAAAATTTCTTTCCCGCATTTCCCGCCTT
AAGCCCGAAGCAGGTGCACG

Downstream 100 bases:

>100_bases
AGCGATCCGCCGCCAAACGGTTTAGCGCAAAAGGCGAAGCGACGAGCTTCGCTTTTTGCACAATCGGCCGGGACCGACGA
ACCCCCGGCCATCGATCGGG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSSHDGLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQSTCAPTNDNLMELMIMVDAL
KRASAGRITAAIPYFGYARQDRRPRSARVAISAKVVANMLEIAGVERIITMDLHADQIQGFFDIPVDNIYATPILLGDLR
KQNYPDLLVVSPDVGGVVRARALAKQLNCDLAIIDKRRPKANVAEVMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKE
RGAKQVFAYATHPVLSGGAADRIAASALDELVVTDTIPLSAESLACPKIRALSSAGLLAETFSRIRRGDSVMSLFAES

Sequences:

>Translated_318_residues
MSSHDGLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQSTCAPTNDNLMELMIMVDAL
KRASAGRITAAIPYFGYARQDRRPRSARVAISAKVVANMLEIAGVERIITMDLHADQIQGFFDIPVDNIYATPILLGDLR
KQNYPDLLVVSPDVGGVVRARALAKQLNCDLAIIDKRRPKANVAEVMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKE
RGAKQVFAYATHPVLSGGAADRIAASALDELVVTDTIPLSAESLACPKIRALSSAGLLAETFSRIRRGDSVMSLFAES
>Mature_317_residues
SSHDGLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQSTCAPTNDNLMELMIMVDALK
RASAGRITAAIPYFGYARQDRRPRSARVAISAKVVANMLEIAGVERIITMDLHADQIQGFFDIPVDNIYATPILLGDLRK
QNYPDLLVVSPDVGGVVRARALAKQLNCDLAIIDKRRPKANVAEVMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKER
GAKQVFAYATHPVLSGGAADRIAASALDELVVTDTIPLSAESLACPKIRALSSAGLLAETFSRIRRGDSVMSLFAES

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=47.4522292993631, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=47.4522292993631, Blast_Score=298, Evalue=6e-81,
Organism=Homo sapiens, GI84875539, Length=316, Percent_Identity=46.8354430379747, Blast_Score=298, Evalue=6e-81,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=47.4522292993631, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI4506133, Length=346, Percent_Identity=36.9942196531792, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=37.6811594202899, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=36.9127516778523, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=36.9127516778523, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=36.9127516778523, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=36.9127516778523, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787458, Length=310, Percent_Identity=63.8709677419355, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=46.4968152866242, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=46.4968152866242, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=46.9453376205788, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=46.4968152866242, Blast_Score=294, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17570245, Length=340, Percent_Identity=34.1176470588235, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321776, Length=313, Percent_Identity=44.408945686901, Blast_Score=265, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6319403, Length=318, Percent_Identity=43.3962264150943, Blast_Score=265, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6320946, Length=319, Percent_Identity=42.6332288401254, Blast_Score=262, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=43.3673469387755, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=108, Percent_Identity=39.8148148148148, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=48.7261146496815, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI45551540, Length=336, Percent_Identity=45.5357142857143, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24651458, Length=359, Percent_Identity=33.4261838440111, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI24651456, Length=359, Percent_Identity=33.4261838440111, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI281362873, Length=359, Percent_Identity=33.4261838440111, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI24651454, Length=359, Percent_Identity=33.4261838440111, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI24651462, Length=207, Percent_Identity=37.6811594202899, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24651464, Length=207, Percent_Identity=37.6811594202899, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI45552010, Length=207, Percent_Identity=37.6811594202899, Blast_Score=141, Evalue=5e-34,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34211; Mature: 34080

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHDGLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQ
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEECCCCCCEEEEEE
STCAPTNDNLMELMIMVDALKRASAGRITAAIPYFGYARQDRRPRSARVAISAKVVANML
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHEEEEHHHHHHHHH
EIAGVERIITMDLHADQIQGFFDIPVDNIYATPILLGDLRKQNYPDLLVVSPDVGGVVRA
HHCCCCEEEEEECCHHHHCCEEECCCCCEEECHHHHHHHHCCCCCCEEEECCCCCHHHHH
RALAKQLNCDLAIIDKRRPKANVAEVMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKE
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
RGAKQVFAYATHPVLSGGAADRIAASALDELVVTDTIPLSAESLACPKIRALSSAGLLAE
CCCCEEEEEECCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHH
TFSRIRRGDSVMSLFAES
HHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
SSHDGLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQ
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEECCCCCCEEEEEE
STCAPTNDNLMELMIMVDALKRASAGRITAAIPYFGYARQDRRPRSARVAISAKVVANML
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHEEEEHHHHHHHHH
EIAGVERIITMDLHADQIQGFFDIPVDNIYATPILLGDLRKQNYPDLLVVSPDVGGVVRA
HHCCCCEEEEEECCHHHHCCEEECCCCCEEECHHHHHHHHCCCCCCEEEECCCCCHHHHH
RALAKQLNCDLAIIDKRRPKANVAEVMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKE
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
RGAKQVFAYATHPVLSGGAADRIAASALDELVVTDTIPLSAESLACPKIRALSSAGLLAE
CCCCEEEEEECCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHH
TFSRIRRGDSVMSLFAES
HHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]