Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 53718147

GI number: 53718147

Start: 556694

End: 558334

Strand: Reverse

Name: leuA [H]

Synonym: BPSL0507

Alternate gene names: 53718147

Gene position: 558334-556694 (Counterclockwise)

Preceding gene: 53718148

Following gene: 53718144

Centisome position: 13.7

GC content: 68.8

Gene sequence:

>1641_bases
ATGAAGCGCAACCCCGCCGAGAAATACCGACCGTTCGAACCCGTTCGCATCAACGGACGCCGATGGCCGAGCCGCGCGAT
CGAGCGCGCGCCGATCTGGATGAGCACCGATCTGCGCGACGGCAACCAGTCGCTGATCGAGCCGATGACGATCGAGCAGA
AGCTCGAATACTTCGAGATGCTCGTCGCGATCGGCTTCAAGGAAATCGAAGTCGGTTTTCCGTCGGCGTCGCAAACCGAC
TTCGATTTCGTGCGCAAGCTGATCGTCGAGCGGCGCATTCCCGACGATGTGACGATCGAAGTGCTCGTGCAATCGCGCGA
AGACCTGATCGCGCGCACGTTCGACGCGCTCGAAGGCGCGCCGCGCGCGATCGTCCACCTGTACAACGCGATCTGCCCGT
CGTTCCGCCGGATCGTGTTCGGGATGTCGAAGGACGACGTGAAGGCGCTCGCGGTGGACGGCACGCGGATCATCAAGGCG
CACGCGCAGGCGCGCCCCGCCACCCACTGGACGTTCCAGTACTCGCCCGAGACGTTCAGCATGACCGAGCTGCCGTTCGC
GCGCGAGGTCTGCGACGCCGTCGCGCAGACGTGGCGGCCGACGCGCGATCACAAGATGATCGTGAACCTGCCCGCCACCG
TCGAGGCCGCGACGCCGAACGTGTTCGCCGACCAGATCGAATGGATGGACCGCAATCTCGGCTATCGCGACAGCATCGTG
CTGTCCGTGCATCCGCACAACGACCGCGGCACGGCGGTCGCGGCGGCCGAGCTCGCGTTGCTCGCGGGCGCGGAGCGCAT
CGAAGGATGCCTGTTCGGCAACGGCGAGCGCACGGGCAACGTCGATCTCGTCACGCTCGCGCTGAACCTGTACACGCAGG
GGATCGATCCGGGCCTCGATTTCTCCGATATCGACGCGGTGCGCCGCGTCGTCGAGCGCTGCAACCGGATTCCCGTGCAT
CCGCGCCATCCGTACGCGGGCGACCTCGTGTTCACCGCGTTCTCCGGCTCGCATCAGGACGCGATCCGCAAGGGCCTCGC
GCAGCAGCGGCCCGATGCGATCTGGGAAGTGCCGTACCTGCCGATCGATCCCGCCGACCTCGGCCGCAGCTACGACGCGG
TGATCCGCGTGAACAGCCAGTCCGGCAAGGGCGGCGCGACCTATCTGCTCGAGCGCGCAATGGGTTTCGCGCCGCCGCGC
CGCGTGCAGATCGAATTCAGCCATGCGGTGCAAACGCTCGCCGACACGTCCGGCGCAGAGGTGACGGCCGATGCGATTTG
CGCGCTCTTCACGCGCGAATATCTGCAGACGATCGGCCCGGCCGCGCGCGCGGGCGCGTCGGCGAGCTGGCACGATCGCG
AAGTCGCGCTCGCGCACGCCGAGGATGCGCCGAGCGCGGCGCGGCAGGCGGCGGCCGCGTTCGCGTCGGCCGCGGGCGTC
GCGATCGACATCACGTCGTGCGAGGTCGAGCGCACCGTGAACGGCGAGACCGCCGTGTTCGTCGGCGCGAGGATCGGCGA
CGCCGCGTTGCGCCACGGCGCGGGCGTGCATCGCGACCCGGTCGCGGCCGCGACGGACGCCGTCGCGAGCGCGATCAACC
GCTCGGCGTGGAGCCGCGACAAGCGGCGCGCCGCGGCCTGA

Upstream 100 bases:

>100_bases
TGCGCGCCGTCGTGCCATCGGGCATGCACGTGCGCAGGCGGCGCCCACCGGCGCGTCATGCCGTCCGTCTTCGCTCGCGA
CTTGACCGCCCGAGGCAAAG

Downstream 100 bases:

>100_bases
AGCGGCGAATTCGAACGGCTTCGGTCGACTTCGAACGGCTTCGAACAGCTTCGAACAGCGTCAGGACGGCTACGGCCGGT
CCGGCGGCGGGCGAACGAAA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKRNPAEKYRPFEPVRINGRRWPSRAIERAPIWMSTDLRDGNQSLIEPMTIEQKLEYFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIVERRIPDDVTIEVLVQSREDLIARTFDALEGAPRAIVHLYNAICPSFRRIVFGMSKDDVKALAVDGTRIIKA
HAQARPATHWTFQYSPETFSMTELPFAREVCDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLGYRDSIV
LSVHPHNDRGTAVAAAELALLAGAERIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVERCNRIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGLAQQRPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATYLLERAMGFAPPR
RVQIEFSHAVQTLADTSGAEVTADAICALFTREYLQTIGPAARAGASASWHDREVALAHAEDAPSAARQAAAAFASAAGV
AIDITSCEVERTVNGETAVFVGARIGDAALRHGAGVHRDPVAAATDAVASAINRSAWSRDKRRAAA

Sequences:

>Translated_546_residues
MKRNPAEKYRPFEPVRINGRRWPSRAIERAPIWMSTDLRDGNQSLIEPMTIEQKLEYFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIVERRIPDDVTIEVLVQSREDLIARTFDALEGAPRAIVHLYNAICPSFRRIVFGMSKDDVKALAVDGTRIIKA
HAQARPATHWTFQYSPETFSMTELPFAREVCDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLGYRDSIV
LSVHPHNDRGTAVAAAELALLAGAERIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVERCNRIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGLAQQRPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATYLLERAMGFAPPR
RVQIEFSHAVQTLADTSGAEVTADAICALFTREYLQTIGPAARAGASASWHDREVALAHAEDAPSAARQAAAAFASAAGV
AIDITSCEVERTVNGETAVFVGARIGDAALRHGAGVHRDPVAAATDAVASAINRSAWSRDKRRAAA
>Mature_546_residues
MKRNPAEKYRPFEPVRINGRRWPSRAIERAPIWMSTDLRDGNQSLIEPMTIEQKLEYFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIVERRIPDDVTIEVLVQSREDLIARTFDALEGAPRAIVHLYNAICPSFRRIVFGMSKDDVKALAVDGTRIIKA
HAQARPATHWTFQYSPETFSMTELPFAREVCDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLGYRDSIV
LSVHPHNDRGTAVAAAELALLAGAERIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVERCNRIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGLAQQRPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATYLLERAMGFAPPR
RVQIEFSHAVQTLADTSGAEVTADAICALFTREYLQTIGPAARAGASASWHDREVALAHAEDAPSAARQAAAAFASAAGV
AIDITSCEVERTVNGETAVFVGARIGDAALRHGAGVHRDPVAAATDAVASAINRSAWSRDKRRAAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=393, Percent_Identity=25.1908396946565, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324225, Length=442, Percent_Identity=56.1085972850679, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324682, Length=442, Percent_Identity=54.2986425339367, Blast_Score=516, Evalue=1e-147,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 59870; Mature: 59870

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNPAEKYRPFEPVRINGRRWPSRAIERAPIWMSTDLRDGNQSLIEPMTIEQKLEYFEM
CCCCCCHHCCCCCCEEECCCCCCHHHHHCCCEEEECCCCCCCHHHHCCHHHHHHHHHHHH
LVAIGFKEIEVGFPSASQTDFDFVRKLIVERRIPDDVTIEVLVQSREDLIARTFDALEGA
HHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
PRAIVHLYNAICPSFRRIVFGMSKDDVKALAVDGTRIIKAHAQARPATHWTFQYSPETFS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHEEEHHHCCCCCEEEEEEECCCCCC
MTELPFAREVCDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLGYRDSIV
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCCCCCEEE
LSVHPHNDRGTAVAAAELALLAGAERIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLD
EEEECCCCCCCHHHHHHHHHHHCHHHHCEEEECCCCCCCCEEEEEEEEHHHHCCCCCCCC
FSDIDAVRRVVERCNRIPVHPRHPYAGDLVFTAFSGSHQDAIRKGLAQQRPDAIWEVPYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEECCCC
PIDPADLGRSYDAVIRVNSQSGKGGATYLLERAMGFAPPRRVQIEFSHAVQTLADTSGAE
CCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCC
VTADAICALFTREYLQTIGPAARAGASASWHDREVALAHAEDAPSAARQAAAAFASAAGV
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCE
AIDITSCEVERTVNGETAVFVGARIGDAALRHGAGVHRDPVAAATDAVASAINRSAWSRD
EEEEECCEEEEECCCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KRRAAA
HHHCCC
>Mature Secondary Structure
MKRNPAEKYRPFEPVRINGRRWPSRAIERAPIWMSTDLRDGNQSLIEPMTIEQKLEYFEM
CCCCCCHHCCCCCCEEECCCCCCHHHHHCCCEEEECCCCCCCHHHHCCHHHHHHHHHHHH
LVAIGFKEIEVGFPSASQTDFDFVRKLIVERRIPDDVTIEVLVQSREDLIARTFDALEGA
HHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
PRAIVHLYNAICPSFRRIVFGMSKDDVKALAVDGTRIIKAHAQARPATHWTFQYSPETFS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHEEEHHHCCCCCEEEEEEECCCCCC
MTELPFAREVCDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLGYRDSIV
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCCCCCEEE
LSVHPHNDRGTAVAAAELALLAGAERIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLD
EEEECCCCCCCHHHHHHHHHHHCHHHHCEEEECCCCCCCCEEEEEEEEHHHHCCCCCCCC
FSDIDAVRRVVERCNRIPVHPRHPYAGDLVFTAFSGSHQDAIRKGLAQQRPDAIWEVPYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEECCCC
PIDPADLGRSYDAVIRVNSQSGKGGATYLLERAMGFAPPRRVQIEFSHAVQTLADTSGAE
CCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCC
VTADAICALFTREYLQTIGPAARAGASASWHDREVALAHAEDAPSAARQAAAAFASAAGV
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCE
AIDITSCEVERTVNGETAVFVGARIGDAALRHGAGVHRDPVAAATDAVASAINRSAWSRD
EEEEECCEEEEECCCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KRRAAA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA