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The map label for this gene is lcfB [H]

Identifier: 53718124

GI number: 53718124

Start: 526780

End: 528348

Strand: Reverse

Name: lcfB [H]

Synonym: BPSL0485

Alternate gene names: 53718124

Gene position: 528348-526780 (Counterclockwise)

Preceding gene: 53718125

Following gene: 53718123

Centisome position: 12.97

GC content: 74.0

Gene sequence:

>1569_bases
ATGAATCCACACATCCGACTCGCCGACCTCCTCGACTCCGCCGCCGCGCAGCGGCCCGACGGCATCGCGATCGCCGAGCC
GCGGCGGCGCGTGCGCTATGCGCAGCTCGCGGCCGACGTGCGCCGCGCGGCCGCCGCGCTCGCCGCGGCCGGCATCGCGC
CCGGCGAGCGCGTCGCGACCTATGCGCCGAAGGCGTACGAGACGATCGTGACGATGCTCGCCGCGAACCTCGCGGGCGCG
ATCGTCGTGCCGATCAATCCGCAGTTGCGCGACCATCAGGTGCGGCACATCCTCGCCGACAGCGGCGCGCGCCTGCTGCT
CACCACCGCGCCGCGCCTCGCCCGGCTCGCCGAGCGCCCGGCCGGGCTCGTCACCTGGCTCGTCGAAGACGTCGCCGCGC
TCGCCGGCGCGGGCGACGACGCGCGCGACGCGGGCCGCGCGCACGCCGTCGATTCCGATCCGGCCGCGCTCCTCTACACG
TCCGGCTCGACCGGGCAGCCGAAGGGCGTCGTGCTGTCGCAGCGCAATCTGACCGCGGGCGCCGACAGCGTCGCCGCATA
CCAGCGGCTCTCGCACGACGACGTGATCCTCGGCGCGCTGCCGCTCAGCTTCGACGCGGGCCTGAGCCAGCTCACGAGCG
CGCTCGCCGCGCAGGCGTGCTACGCGCCGCTCGATTTCCTGCGCGCGGAGGAAGTGCCCGCGTGGTGCGCGCAAGTCGGC
GTCACGTCGATCACCGGCGTGCCGCCGCTCTGGATGCAGCTCGCCGCGGTCGCATGGCCCGACGCCGCGCGCCTGCCGGT
GCGCCGGATCGCGAACACGGGCGGCACGATGCCGCAGCCGCTGCTGCACCGGCTGCGGCAGATCTTTCCGAACGCGGCGC
CGTACCTGATGTACGGCCTCACCGAGGCGTTCCGCTCGACATATCTGCCGCCGGAGGAAGCGGCCGCGCGCCCCGGCTCG
ATCGGCAAGGCCGTGCCGAATGCGCAGATCCTCGTGCTGCGCGCCGACGGCAGCGAGTGCGACGCCGGCGAGCCGGGCGA
GCTCGTGCATCGCGGCGCGTTCGTGACGCTCGGCTACTGGAACGCGCCCGAGCTGACCGCGCAGCGCTTTCGCGCGCTGC
CGCACGCGCGCCATCCGGTGCCGCTCGCGGACGTCGCGGTGTGGTCGGGCGACATCGTGACGCGCGACGACGAAGGCTTC
CTCTATTTCGTGTCGCGCGCCGACGAGATGATCAAGACGTCCGGCTACCGCGTGAGCCCGACCGAGATCGAGGACATCCT
GTTCGAATGCCCGCAGACGCTCGAAGCCGTCGCGTTCGGCGTGCCGCATCCGGCGCTCGGCCAGGCGGTCGTCGCGTGCG
TGTACGGCGGCGGCGACCCCGCGTCATGCCGGCAGGCGCTGCTCGACGCGTGCCGCGCGCGGCTGCCGTCGTACATGGTG
CCGCAGCACATCGAGATCGCCGGCGCGCCGCTGCCGCGCAATCCGAACGGCAAGATCGACCGGCCGCTGCTCAAGCGCGC
CCACCTGTCGCGTTTCGAGGCCGAGCCGCGCGCGGCCGCGCTCGGCTGA

Upstream 100 bases:

>100_bases
GTGTTCTCGCAATGCGGCGCCTATCACCACTCGTACACCGTGAGCGGCTTCCTGTCGCACAAGCCGGCTCAGGTCTACAT
CCGTCAGGCATGAGAGCGTC

Downstream 100 bases:

>100_bases
GCGAGGGGACGAAGACCATGCTCACGATCCATCACACCGCGATCCGGCAGCCGTCCCGCCGGTCGGTCGACGCCGCGCAG
GCGAGCGGCCGCTATCGCTA

Product: putative AMP-binding enzyme

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MNPHIRLADLLDSAAAQRPDGIAIAEPRRRVRYAQLAADVRRAAAALAAAGIAPGERVATYAPKAYETIVTMLAANLAGA
IVVPINPQLRDHQVRHILADSGARLLLTTAPRLARLAERPAGLVTWLVEDVAALAGAGDDARDAGRAHAVDSDPAALLYT
SGSTGQPKGVVLSQRNLTAGADSVAAYQRLSHDDVILGALPLSFDAGLSQLTSALAAQACYAPLDFLRAEEVPAWCAQVG
VTSITGVPPLWMQLAAVAWPDAARLPVRRIANTGGTMPQPLLHRLRQIFPNAAPYLMYGLTEAFRSTYLPPEEAAARPGS
IGKAVPNAQILVLRADGSECDAGEPGELVHRGAFVTLGYWNAPELTAQRFRALPHARHPVPLADVAVWSGDIVTRDDEGF
LYFVSRADEMIKTSGYRVSPTEIEDILFECPQTLEAVAFGVPHPALGQAVVACVYGGGDPASCRQALLDACRARLPSYMV
PQHIEIAGAPLPRNPNGKIDRPLLKRAHLSRFEAEPRAAALG

Sequences:

>Translated_522_residues
MNPHIRLADLLDSAAAQRPDGIAIAEPRRRVRYAQLAADVRRAAAALAAAGIAPGERVATYAPKAYETIVTMLAANLAGA
IVVPINPQLRDHQVRHILADSGARLLLTTAPRLARLAERPAGLVTWLVEDVAALAGAGDDARDAGRAHAVDSDPAALLYT
SGSTGQPKGVVLSQRNLTAGADSVAAYQRLSHDDVILGALPLSFDAGLSQLTSALAAQACYAPLDFLRAEEVPAWCAQVG
VTSITGVPPLWMQLAAVAWPDAARLPVRRIANTGGTMPQPLLHRLRQIFPNAAPYLMYGLTEAFRSTYLPPEEAAARPGS
IGKAVPNAQILVLRADGSECDAGEPGELVHRGAFVTLGYWNAPELTAQRFRALPHARHPVPLADVAVWSGDIVTRDDEGF
LYFVSRADEMIKTSGYRVSPTEIEDILFECPQTLEAVAFGVPHPALGQAVVACVYGGGDPASCRQALLDACRARLPSYMV
PQHIEIAGAPLPRNPNGKIDRPLLKRAHLSRFEAEPRAAALG
>Mature_522_residues
MNPHIRLADLLDSAAAQRPDGIAIAEPRRRVRYAQLAADVRRAAAALAAAGIAPGERVATYAPKAYETIVTMLAANLAGA
IVVPINPQLRDHQVRHILADSGARLLLTTAPRLARLAERPAGLVTWLVEDVAALAGAGDDARDAGRAHAVDSDPAALLYT
SGSTGQPKGVVLSQRNLTAGADSVAAYQRLSHDDVILGALPLSFDAGLSQLTSALAAQACYAPLDFLRAEEVPAWCAQVG
VTSITGVPPLWMQLAAVAWPDAARLPVRRIANTGGTMPQPLLHRLRQIFPNAAPYLMYGLTEAFRSTYLPPEEAAARPGS
IGKAVPNAQILVLRADGSECDAGEPGELVHRGAFVTLGYWNAPELTAQRFRALPHARHPVPLADVAVWSGDIVTRDDEGF
LYFVSRADEMIKTSGYRVSPTEIEDILFECPQTLEAVAFGVPHPALGQAVVACVYGGGDPASCRQALLDACRARLPSYMV
PQHIEIAGAPLPRNPNGKIDRPLLKRAHLSRFEAEPRAAALG

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=549, Percent_Identity=21.8579234972678, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI38505220, Length=508, Percent_Identity=25.3937007874016, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI187761345, Length=545, Percent_Identity=24.4036697247706, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI187761343, Length=545, Percent_Identity=24.4036697247706, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI157311624, Length=513, Percent_Identity=24.1715399610136, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI157311622, Length=513, Percent_Identity=24.1715399610136, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI122937307, Length=545, Percent_Identity=23.4862385321101, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI58082049, Length=509, Percent_Identity=23.5756385068762, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI42544132, Length=542, Percent_Identity=21.9557195571956, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI115511026, Length=524, Percent_Identity=23.0916030534351, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788107, Length=546, Percent_Identity=28.7545787545788, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=27.5280898876405, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1786801, Length=451, Percent_Identity=28.159645232816, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI221142682, Length=509, Percent_Identity=27.3084479371316, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1786810, Length=544, Percent_Identity=23.8970588235294, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789201, Length=363, Percent_Identity=26.7217630853994, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=24.7011952191235, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=550, Percent_Identity=23.4545454545455, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=550, Percent_Identity=23.4545454545455, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=550, Percent_Identity=23.4545454545455, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17558820, Length=501, Percent_Identity=25.3493013972056, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17560308, Length=532, Percent_Identity=22.5563909774436, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=549, Percent_Identity=24.408014571949, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=558, Percent_Identity=24.0143369175627, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=554, Percent_Identity=24.7292418772563, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=540, Percent_Identity=23.3333333333333, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=467, Percent_Identity=23.3404710920771, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6319699, Length=484, Percent_Identity=24.7933884297521, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24581924, Length=544, Percent_Identity=26.2867647058824, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI18859661, Length=518, Percent_Identity=27.2200772200772, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21355181, Length=512, Percent_Identity=25, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356947, Length=488, Percent_Identity=21.7213114754098, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648260, Length=486, Percent_Identity=22.8395061728395, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356441, Length=356, Percent_Identity=25.561797752809, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281365686, Length=373, Percent_Identity=23.0563002680965, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648255, Length=372, Percent_Identity=26.6129032258064, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076582, Length=493, Percent_Identity=23.5294117647059, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648253, Length=364, Percent_Identity=26.3736263736264, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653035, Length=487, Percent_Identity=23.4086242299795, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20130357, Length=409, Percent_Identity=23.7163814180929, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648257, Length=453, Percent_Identity=23.3995584988962, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 55466; Mature: 55466

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPHIRLADLLDSAAAQRPDGIAIAEPRRRVRYAQLAADVRRAAAALAAAGIAPGERVAT
CCCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
YAPKAYETIVTMLAANLAGAIVVPINPQLRDHQVRHILADSGARLLLTTAPRLARLAERP
CCCHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCC
AGLVTWLVEDVAALAGAGDDARDAGRAHAVDSDPAALLYTSGSTGQPKGVVLSQRNLTAG
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCC
ADSVAAYQRLSHDDVILGALPLSFDAGLSQLTSALAAQACYAPLDFLRAEEVPAWCAQVG
CHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
VTSITGVPPLWMQLAAVAWPDAARLPVRRIANTGGTMPQPLLHRLRQIFPNAAPYLMYGL
CHHCCCCCHHHHHHHHHCCCCHHCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
TEAFRSTYLPPEEAAARPGSIGKAVPNAQILVLRADGSECDAGEPGELVHRGAFVTLGYW
HHHHHHCCCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCEEEEEECC
NAPELTAQRFRALPHARHPVPLADVAVWSGDIVTRDDEGFLYFVSRADEMIKTSGYRVSP
CCCHHHHHHHHHCCCCCCCCCHHHHHEECCCEEEECCCCCEEEEEHHHHHHHHCCCCCCH
TEIEDILFECPQTLEAVAFGVPHPALGQAVVACVYGGGDPASCRQALLDACRARLPSYMV
HHHHHHHHHCHHHHHHHHHCCCCHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCC
PQHIEIAGAPLPRNPNGKIDRPLLKRAHLSRFEAEPRAAALG
CCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure
MNPHIRLADLLDSAAAQRPDGIAIAEPRRRVRYAQLAADVRRAAAALAAAGIAPGERVAT
CCCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
YAPKAYETIVTMLAANLAGAIVVPINPQLRDHQVRHILADSGARLLLTTAPRLARLAERP
CCCHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCC
AGLVTWLVEDVAALAGAGDDARDAGRAHAVDSDPAALLYTSGSTGQPKGVVLSQRNLTAG
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCC
ADSVAAYQRLSHDDVILGALPLSFDAGLSQLTSALAAQACYAPLDFLRAEEVPAWCAQVG
CHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
VTSITGVPPLWMQLAAVAWPDAARLPVRRIANTGGTMPQPLLHRLRQIFPNAAPYLMYGL
CHHCCCCCHHHHHHHHHCCCCHHCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
TEAFRSTYLPPEEAAARPGSIGKAVPNAQILVLRADGSECDAGEPGELVHRGAFVTLGYW
HHHHHHCCCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCEEEEEECC
NAPELTAQRFRALPHARHPVPLADVAVWSGDIVTRDDEGFLYFVSRADEMIKTSGYRVSP
CCCHHHHHHHHHCCCCCCCCCHHHHHEECCCEEEECCCCCEEEEEHHHHHHHHCCCCCCH
TEIEDILFECPQTLEAVAFGVPHPALGQAVVACVYGGGDPASCRQALLDACRARLPSYMV
HHHHHHHHHCHHHHHHHHHCCCCHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCC
PQHIEIAGAPLPRNPNGKIDRPLLKRAHLSRFEAEPRAAALG
CCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]