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The map label for this gene is trmL [H]

Identifier: 53718088

GI number: 53718088

Start: 488218

End: 488688

Strand: Reverse

Name: trmL [H]

Synonym: BPSL0449

Alternate gene names: 53718088

Gene position: 488688-488218 (Counterclockwise)

Preceding gene: 53718089

Following gene: 53718082

Centisome position: 11.99

GC content: 68.79

Gene sequence:

>471_bases
ATGTTCAACGTCGTTCTCGTCGAGCCCGAAATTCCGCCGAACACCGGCAACGTGATCCGGCTGTGCGCCAATACGGGCGC
GCGGCTGCATCTGATCGAGCCGCTCGGCTTTCCGCTCGACGACGCGAAAATGCGGCGCGCGGGGCTCGACTACCACGAAT
ACGCGCAGATGCGCGTGCATCGCGACTGGGACGCGTTCGTCGCCGCCGAGGCGCCCGATCCCGCGCGGATGTTCGCGTTC
ACGACGCGCGGCTCGGGCCGCTTTCACGACCGCTCATTCGAGCCGGGCGACTGGTTCGTGTTCGGCGCGGAGACGCGCGG
GCTCGCGCCCGCGCTCGTCGATCGCTTCGCTCCCGAGCAGCGCGTGCGGCTGCCGATGCGGCCGGGCAATCGCAGCCTCA
ATCTGTCGAACACGGTTGCGGTCGTCGTGTTCGAGGCATGGCGCCAGGCGGGGTTCGAAGGCGGGGCTTGA

Upstream 100 bases:

>100_bases
CGGCGCGTGACGAACTTCGTCGCGCTGCGCACCGCGAAGGATTGACGGCGCGCCGCATCCGCCGCCGCATTCCCACGCTT
TTCACCGATCGAATCGAATC

Downstream 100 bases:

>100_bases
ACAGGGGCGACCGACGTCCGCCCGGCGGGACGCACGCGACTACGCGGCAATTGAGCAGCAACTGAGCAGCGACGGAGCAG
GGCGCCGCCGCGCAGCGGCA

Product: SpoU rRNA methylase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MFNVVLVEPEIPPNTGNVIRLCANTGARLHLIEPLGFPLDDAKMRRAGLDYHEYAQMRVHRDWDAFVAAEAPDPARMFAF
TTRGSGRFHDRSFEPGDWFVFGAETRGLAPALVDRFAPEQRVRLPMRPGNRSLNLSNTVAVVVFEAWRQAGFEGGA

Sequences:

>Translated_156_residues
MFNVVLVEPEIPPNTGNVIRLCANTGARLHLIEPLGFPLDDAKMRRAGLDYHEYAQMRVHRDWDAFVAAEAPDPARMFAF
TTRGSGRFHDRSFEPGDWFVFGAETRGLAPALVDRFAPEQRVRLPMRPGNRSLNLSNTVAVVVFEAWRQAGFEGGA
>Mature_156_residues
MFNVVLVEPEIPPNTGNVIRLCANTGARLHLIEPLGFPLDDAKMRRAGLDYHEYAQMRVHRDWDAFVAAEAPDPARMFAF
TTRGSGRFHDRSFEPGDWFVFGAETRGLAPALVDRFAPEQRVRLPMRPGNRSLNLSNTVAVVVFEAWRQAGFEGGA

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=155, Percent_Identity=56.7741935483871, Blast_Score=193, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17469; Mature: 17469

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNVVLVEPEIPPNTGNVIRLCANTGARLHLIEPLGFPLDDAKMRRAGLDYHEYAQMRVH
CCEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHEEEE
RDWDAFVAAEAPDPARMFAFTTRGSGRFHDRSFEPGDWFVFGAETRGLAPALVDRFAPEQ
CCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCH
RVRLPMRPGNRSLNLSNTVAVVVFEAWRQAGFEGGA
HEECCCCCCCCCEECCCEEEEEEEHHHHHCCCCCCC
>Mature Secondary Structure
MFNVVLVEPEIPPNTGNVIRLCANTGARLHLIEPLGFPLDDAKMRRAGLDYHEYAQMRVH
CCEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHEEEE
RDWDAFVAAEAPDPARMFAFTTRGSGRFHDRSFEPGDWFVFGAETRGLAPALVDRFAPEQ
CCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCH
RVRLPMRPGNRSLNLSNTVAVVVFEAWRQAGFEGGA
HEECCCCCCCCCEECCCEEEEEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA