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The map label for this gene is cpdB [H]

Identifier: 53718040

GI number: 53718040

Start: 433552

End: 435612

Strand: Reverse

Name: cpdB [H]

Synonym: BPSL0400

Alternate gene names: 53718040

Gene position: 435612-433552 (Counterclockwise)

Preceding gene: 53718051

Following gene: 53718039

Centisome position: 10.69

GC content: 67.35

Gene sequence:

>2061_bases
ATGCCATTCCCCTTGCGTTTTCGCCGGCGGCGCCTTGCCGCCGCCCTCGTCGCGTGCGCGGCGCTCGTCGCGGGCTGCAA
CGACGACGTCGATTCGCCCGGCGCGCAGTCCGGCGCGAGCGCGCCCGCCGGCACGAAGGCGACGCTCGCCGTGCTCGAGA
CGACCGATCTGCACACGAACGTGCTGTCGTACGACTACTTCAAGCTCGCGGCCGACAAGTCGCTCGGCTTCGAGCGGGTC
GCGACGCTGATCGCGCAGGCGCGCGCGCAATATCCGAACACGCTGCTGCTCGATAACGGCGACACGATCCAGGGCACGGC
GCTCGCCGACTATCAGGCGCTCGTGAAGCCCGTGTCGTGCGGCGAGACGCTTGCGATCTACAAGGTGATGAACGCGGCGA
AGTTCGACGGCGGCGGCATCGGCAATCACGAATTCAACTACGGGCTGCCGTACCTGTCGCAGGTGACGGGCAACGCGTTC
GCCGTCGACGGCCTGCCCGATCCGGCCCGGCAGAAGAAATGCGCGGGGCCGGACTTCCCGCAGGTGCTCGCGAACGTGAT
CAGCGCGAAGACGAACGCGCCCCTCTTCACGCCGTACACGATCCTCACGAAGAACGTCACCGCGACGACGCCCGACGGCC
GCACGGTCACGGCGCCCGTGAAGGTCGGCATCATCGGCTTCACGCCGCCCGCGATCATGAACTGGGACAAGCGCTGGCTC
GACGGCAAGGTCTATACGACGGGCCTGAAGGAAGCCGCCGAGAAGTACATTCCCGAGATGCGCGCGAAGGGCGCGGACCT
CGTCGTCGCGATCTCGCACGGCGGGCTCGACAACTCGCCGTACTCGCCGACGATGGAAAACGGCAGCTGGTGGCTGTCCA
CCGTGCCCGGCATCGACGCGATGCTGATCGGCCACTCGCACCAGGTGTTCCCCGACGCGACGAGCACCGTCGCGCAGTTC
AACCTGCCGGGCGTCGACAAGGTCAAGGGCACGGTCAACGGCGTGCCGACCGTGATGGCGAACTACTGGGGCAAGCACCT
CGGCGTGATCAAGCTCGGCCTCGCGTTCGACGGCAAGACGTGGCGCGTCGACAAATCGCAGACCACCGTCGAGGCGCGCT
CGATCCAGAACCCGGACAAGAGCTACGTCGACGCCGATCCGTCGGTGGCCGCGGCGATCGCGGCCGAGCACCAGGCGACG
ATCGACTACGTGAAGACGCCGATCGGCAGCACCGACTACCGGATGACCAGCTACTTCGCGGACGTGGGCGATCCGGGCGC
GATCCAGCTCGTCAACGAGGCGCAGGCCGACTACGTCGCGAACTACGTGCAGACGAACCTGCCGCAGTATGCGTCGCTGC
CGGTGCTGTCGGTGAGCGCGCCGTTCAAGAGCGGCTTCGGCGGCGGCACCGATTTCACCGACGTCGCGGCGGGCGCGCTC
GCGATCAACAACGCGGCCGATCTCTACCTGTATCCGAACACGGTCTACGCGGTGAAGGTGAGCGGCGCGGACATCAAGAA
CTGGCTCGAGACCGCGGCGAAGCGCTTCAACACGATCGACCCGACGAAAGCGACCGTGCAGAAGCTCGTGAGCACGTTCC
CCGGCTACAACTTCGACATGTTCACATCCGCCGATCTGCGCTACGAGATCGACGTCACGCAGCCGGCGGGCAGCCGGATC
CGCAACCTCGCGTACAAGGGCGCGCCGATCGATCCGAATGCGCAGTTCATCGTCGCGACGAACAACTACCGCGCGAGCGG
CGGCGGCAACTTCCCCGGCCTGGACGGCAGCAAGACGATCTTCGCGTCGCCCGACGCGAACCGCGACGTGCTGATCGCAT
ACATCAAGAAGCGCGGCCGGATCACGCGCGCGGCGGACGGCATGCAGCGCAGCTGGCGCTTCACGAAGCTCGCGGGCTCG
GTCGCGCACGTGCAGTTCGCGTCGGCGCCGAACCTCGCGGCGCTCGCGAGCACCGCGGGCCTCGCGGGCATCACGCAAGT
CGCGGCCGACGACGGCTCCGGCAAGGGACTCGCGACTTACGAGATCGATCTGACGCAATGA

Upstream 100 bases:

>100_bases
GTTTCCTTTCGATACTCTCACGCGCTGGATGTCAGGCCGTCGGCGCATGCGCCGCTCACGCCATCCCCGGCTCAACGGAA
ACAGAACAGGAAAAATCCCG

Downstream 100 bases:

>100_bases
CGCAGCGGGCGGCGCGCCGCCGGAATGCCGCCGCGCGCCGCCCGCCCGACGCACCTGAAACCGATTCGCCCCGATGATGA
ACGCAATCAAGAAGCTGCTC

Product: putative 2',3'-cyclic-nucleotide 2'-phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MPFPLRFRRRRLAAALVACAALVAGCNDDVDSPGAQSGASAPAGTKATLAVLETTDLHTNVLSYDYFKLAADKSLGFERV
ATLIAQARAQYPNTLLLDNGDTIQGTALADYQALVKPVSCGETLAIYKVMNAAKFDGGGIGNHEFNYGLPYLSQVTGNAF
AVDGLPDPARQKKCAGPDFPQVLANVISAKTNAPLFTPYTILTKNVTATTPDGRTVTAPVKVGIIGFTPPAIMNWDKRWL
DGKVYTTGLKEAAEKYIPEMRAKGADLVVAISHGGLDNSPYSPTMENGSWWLSTVPGIDAMLIGHSHQVFPDATSTVAQF
NLPGVDKVKGTVNGVPTVMANYWGKHLGVIKLGLAFDGKTWRVDKSQTTVEARSIQNPDKSYVDADPSVAAAIAAEHQAT
IDYVKTPIGSTDYRMTSYFADVGDPGAIQLVNEAQADYVANYVQTNLPQYASLPVLSVSAPFKSGFGGGTDFTDVAAGAL
AINNAADLYLYPNTVYAVKVSGADIKNWLETAAKRFNTIDPTKATVQKLVSTFPGYNFDMFTSADLRYEIDVTQPAGSRI
RNLAYKGAPIDPNAQFIVATNNYRASGGGNFPGLDGSKTIFASPDANRDVLIAYIKKRGRITRAADGMQRSWRFTKLAGS
VAHVQFASAPNLAALASTAGLAGITQVAADDGSGKGLATYEIDLTQ

Sequences:

>Translated_686_residues
MPFPLRFRRRRLAAALVACAALVAGCNDDVDSPGAQSGASAPAGTKATLAVLETTDLHTNVLSYDYFKLAADKSLGFERV
ATLIAQARAQYPNTLLLDNGDTIQGTALADYQALVKPVSCGETLAIYKVMNAAKFDGGGIGNHEFNYGLPYLSQVTGNAF
AVDGLPDPARQKKCAGPDFPQVLANVISAKTNAPLFTPYTILTKNVTATTPDGRTVTAPVKVGIIGFTPPAIMNWDKRWL
DGKVYTTGLKEAAEKYIPEMRAKGADLVVAISHGGLDNSPYSPTMENGSWWLSTVPGIDAMLIGHSHQVFPDATSTVAQF
NLPGVDKVKGTVNGVPTVMANYWGKHLGVIKLGLAFDGKTWRVDKSQTTVEARSIQNPDKSYVDADPSVAAAIAAEHQAT
IDYVKTPIGSTDYRMTSYFADVGDPGAIQLVNEAQADYVANYVQTNLPQYASLPVLSVSAPFKSGFGGGTDFTDVAAGAL
AINNAADLYLYPNTVYAVKVSGADIKNWLETAAKRFNTIDPTKATVQKLVSTFPGYNFDMFTSADLRYEIDVTQPAGSRI
RNLAYKGAPIDPNAQFIVATNNYRASGGGNFPGLDGSKTIFASPDANRDVLIAYIKKRGRITRAADGMQRSWRFTKLAGS
VAHVQFASAPNLAALASTAGLAGITQVAADDGSGKGLATYEIDLTQ
>Mature_685_residues
PFPLRFRRRRLAAALVACAALVAGCNDDVDSPGAQSGASAPAGTKATLAVLETTDLHTNVLSYDYFKLAADKSLGFERVA
TLIAQARAQYPNTLLLDNGDTIQGTALADYQALVKPVSCGETLAIYKVMNAAKFDGGGIGNHEFNYGLPYLSQVTGNAFA
VDGLPDPARQKKCAGPDFPQVLANVISAKTNAPLFTPYTILTKNVTATTPDGRTVTAPVKVGIIGFTPPAIMNWDKRWLD
GKVYTTGLKEAAEKYIPEMRAKGADLVVAISHGGLDNSPYSPTMENGSWWLSTVPGIDAMLIGHSHQVFPDATSTVAQFN
LPGVDKVKGTVNGVPTVMANYWGKHLGVIKLGLAFDGKTWRVDKSQTTVEARSIQNPDKSYVDADPSVAAAIAAEHQATI
DYVKTPIGSTDYRMTSYFADVGDPGAIQLVNEAQADYVANYVQTNLPQYASLPVLSVSAPFKSGFGGGTDFTDVAAGALA
INNAADLYLYPNTVYAVKVSGADIKNWLETAAKRFNTIDPTKATVQKLVSTFPGYNFDMFTSADLRYEIDVTQPAGSRIR
NLAYKGAPIDPNAQFIVATNNYRASGGGNFPGLDGSKTIFASPDANRDVLIAYIKKRGRITRAADGMQRSWRFTKLAGSV
AHVQFASAPNLAALASTAGLAGITQVAADDGSGKGLATYEIDLTQ

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=664, Percent_Identity=43.0722891566265, Blast_Score=487, Evalue=1e-138,
Organism=Escherichia coli, GI1786687, Length=591, Percent_Identity=22.8426395939086, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 72862; Mature: 72731

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFPLRFRRRRLAAALVACAALVAGCNDDVDSPGAQSGASAPAGTKATLAVLETTDLHTN
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHH
VLSYDYFKLAADKSLGFERVATLIAQARAQYPNTLLLDNGDTIQGTALADYQALVKPVSC
HHCCCHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEECCCHHHHHHHHHHCCC
GETLAIYKVMNAAKFDGGGIGNHEFNYGLPYLSQVTGNAFAVDGLPDPARQKKCAGPDFP
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCHHHHCCCCCCCHH
QVLANVISAKTNAPLFTPYTILTKNVTATTPDGRTVTAPVKVGIIGFTPPAIMNWDKRWL
HHHHHHHHCCCCCCCCCCCEEEECCEEEECCCCCEEECCEEEEEEECCCHHHCCCCHHHC
DGKVYTTGLKEAAEKYIPEMRAKGADLVVAISHGGLDNSPYSPTMENGSWWLSTVPGIDA
CCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEE
MLIGHSHQVFPDATSTVAQFNLPGVDKVKGTVNGVPTVMANYWGKHLGVIKLGLAFDGKT
EEECCCCCCCCCCHHHHEECCCCCHHHHCCCCCCCHHHHHHHHHHHCCEEEEEEEECCCE
WRVDKSQTTVEARSIQNPDKSYVDADPSVAAAIAAEHQATIDYVKTPIGSTDYRMTSYFA
EEECCCCCEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
DVGDPGAIQLVNEAQADYVANYVQTNLPQYASLPVLSVSAPFKSGFGGGTDFTDVAAGAL
CCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHCCCEEEECCCCCCCCCCCCCHHHHHHHHE
AINNAADLYLYPNTVYAVKVSGADIKNWLETAAKRFNTIDPTKATVQKLVSTFPGYNFDM
EECCCCEEEEECCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEE
FTSADLRYEIDVTQPAGSRIRNLAYKGAPIDPNAQFIVATNNYRASGGGNFPGLDGSKTI
EECCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEEECCEECCCCCCCCCCCCCCEE
FASPDANRDVLIAYIKKRGRITRAADGMQRSWRFTKLAGSVAHVQFASAPNLAALASTAG
EECCCCCCCEEEEEEHHCCCEEHHHCCHHHCCCHHHHCCCEEEEEECCCCCHHHHHHHCC
LAGITQVAADDGSGKGLATYEIDLTQ
HHHHHHEECCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
PFPLRFRRRRLAAALVACAALVAGCNDDVDSPGAQSGASAPAGTKATLAVLETTDLHTN
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHH
VLSYDYFKLAADKSLGFERVATLIAQARAQYPNTLLLDNGDTIQGTALADYQALVKPVSC
HHCCCHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEECCCHHHHHHHHHHCCC
GETLAIYKVMNAAKFDGGGIGNHEFNYGLPYLSQVTGNAFAVDGLPDPARQKKCAGPDFP
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCHHHHCCCCCCCHH
QVLANVISAKTNAPLFTPYTILTKNVTATTPDGRTVTAPVKVGIIGFTPPAIMNWDKRWL
HHHHHHHHCCCCCCCCCCCEEEECCEEEECCCCCEEECCEEEEEEECCCHHHCCCCHHHC
DGKVYTTGLKEAAEKYIPEMRAKGADLVVAISHGGLDNSPYSPTMENGSWWLSTVPGIDA
CCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEE
MLIGHSHQVFPDATSTVAQFNLPGVDKVKGTVNGVPTVMANYWGKHLGVIKLGLAFDGKT
EEECCCCCCCCCCHHHHEECCCCCHHHHCCCCCCCHHHHHHHHHHHCCEEEEEEEECCCE
WRVDKSQTTVEARSIQNPDKSYVDADPSVAAAIAAEHQATIDYVKTPIGSTDYRMTSYFA
EEECCCCCEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
DVGDPGAIQLVNEAQADYVANYVQTNLPQYASLPVLSVSAPFKSGFGGGTDFTDVAAGAL
CCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHCCCEEEECCCCCCCCCCCCCHHHHHHHHE
AINNAADLYLYPNTVYAVKVSGADIKNWLETAAKRFNTIDPTKATVQKLVSTFPGYNFDM
EECCCCEEEEECCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEE
FTSADLRYEIDVTQPAGSRIRNLAYKGAPIDPNAQFIVATNNYRASGGGNFPGLDGSKTI
EECCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEEECCEECCCCCCCCCCCCCCEE
FASPDANRDVLIAYIKKRGRITRAADGMQRSWRFTKLAGSVAHVQFASAPNLAALASTAG
EECCCCCCCEEEEEEHHCCCEEHHHCCHHHCCCHHHHCCCEEEEEECCCCCHHHHHHHCC
LAGITQVAADDGSGKGLATYEIDLTQ
HHHHHHEECCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3005231; 7610040; 9278503; 2172762; 9298646 [H]