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The map label for this gene is thlA [H]

Identifier: 53718011

GI number: 53718011

Start: 400265

End: 401458

Strand: Reverse

Name: thlA [H]

Synonym: BPSL0371

Alternate gene names: 53718011

Gene position: 401458-400265 (Counterclockwise)

Preceding gene: 53718012

Following gene: 53718010

Centisome position: 9.85

GC content: 71.36

Gene sequence:

>1194_bases
ATGACGGCTGTTGATCAGGATCCGATCGTCATCGCATCGGCGGCGCGCACGCCGATCGCGGGCTTTCAGGGCGAGTTCGC
GTCGCTCGCCGCGCCGCAGCTGGGCGCGGCCGCGATCGCCGCGGCGCTCGAGCGCGCGGGCCTGCAACCGGAGCAGATCG
ACGAGGCGGTGATGGGCTGCGTGCTGCCCGCCGGGCAGGGGCAGGCGCCGGCACGGCAGGCGGCGCTCGGCGCGAAGCTG
CCGCTGTCGGTCGGCTGCACGACGATCAACAAGATGTGCGGCTCGGGCATGCGCGCGGCGATGTTCGCGCACGACATGCT
CGTGGCGGGCTCCGTCGACGTGATCGTCGCGGGCGGCATGGAAAGCATGACGAACGCGCCGTACCTGCTGCCGAAGGCGC
GCGCCGGCATGCGCATGGGCCACGGCCAGGTGCTCGACCACATGTTCCTCGACGGCCTCGAGGACGCCTACGACAAGGGC
CGGCTGATGGGCACGTTCGCCGAGGAATGCGCGGGCGAATACGCGTTCTCGCGCGACGCGCAGGACGCGTTCGCGATCGA
ATCGCTCGCGCGCGCGAAGCGGGCGAACGAGGACGGCTCGTTCGCGTGGGAAATCGCGCCCGTGACGGTGGCCGGCAAGA
AGGGCGATGCGGTGATCGCGCGCGACGAGCAGCCGTTCAAGGCGAACCCCGAGAAGATTCCGACGCTCAAGCCCGCGTTC
AGCAAGACGGGCACCGTGACCGCCGCGAATTCGTCGTCGATCTCCGACGGCGCGGCCGCGCTCGTGATGATGCGCGCGTC
GACGGCGAACCGGCTCGGGCTCGCGCCGCTCGCGCGCGTCGTCGGCCATTCGACGTTCGCGCAGGCGCCGTCGAAGTTCA
CGACGGCGCCTGTCGGCGCGATCCGGCGCCTGTTCGAGAAGAACGGCTGGCGCGCGGCCGAGGTCGACCTGTACGAGATC
AACGAAGCGTTCGCGGTCGTCACGATGGCGGCGATGAAGGAGCACGGCCTGCCGCACGAGAAGGTCAACGTGAACGGCGG
CGCGTGCGCGCTCGGCCATCCGATCGGCGCGTCGGGCGCCCGCATCCTCGTCACGCTGATCGGCGCGCTGCGCGCGCGCG
GGGCCAAGCGCGGCGTGGCGAGCCTGTGCATCGGCGGCGGCGAAGCGACCGCGATGGGCATCGAGCTGATCTGA

Upstream 100 bases:

>100_bases
AGCGGCGCGCATTCGGCGGACAATGACGTCGTGGCGGCCGACGCGGCGCAACTCGCCGGCGCGGTCGATCTCATTGCCCA
AACCATCAAGGAGACGAAAC

Downstream 100 bases:

>100_bases
CCTGAGCGCGCATCGAACGGGAGCCCTCCGATGAAGACCGTATTGATCGTCGGCGCATCGCGCGGCATCGGCCGCGAATT
CGTCGGCCAGTATCTGCATG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTAVDQDPIVIASAARTPIAGFQGEFASLAAPQLGAAAIAAALERAGLQPEQIDEAVMGCVLPAGQGQAPARQAALGAKL
PLSVGCTTINKMCGSGMRAAMFAHDMLVAGSVDVIVAGGMESMTNAPYLLPKARAGMRMGHGQVLDHMFLDGLEDAYDKG
RLMGTFAEECAGEYAFSRDAQDAFAIESLARAKRANEDGSFAWEIAPVTVAGKKGDAVIARDEQPFKANPEKIPTLKPAF
SKTGTVTAANSSSISDGAAALVMMRASTANRLGLAPLARVVGHSTFAQAPSKFTTAPVGAIRRLFEKNGWRAAEVDLYEI
NEAFAVVTMAAMKEHGLPHEKVNVNGGACALGHPIGASGARILVTLIGALRARGAKRGVASLCIGGGEATAMGIELI

Sequences:

>Translated_397_residues
MTAVDQDPIVIASAARTPIAGFQGEFASLAAPQLGAAAIAAALERAGLQPEQIDEAVMGCVLPAGQGQAPARQAALGAKL
PLSVGCTTINKMCGSGMRAAMFAHDMLVAGSVDVIVAGGMESMTNAPYLLPKARAGMRMGHGQVLDHMFLDGLEDAYDKG
RLMGTFAEECAGEYAFSRDAQDAFAIESLARAKRANEDGSFAWEIAPVTVAGKKGDAVIARDEQPFKANPEKIPTLKPAF
SKTGTVTAANSSSISDGAAALVMMRASTANRLGLAPLARVVGHSTFAQAPSKFTTAPVGAIRRLFEKNGWRAAEVDLYEI
NEAFAVVTMAAMKEHGLPHEKVNVNGGACALGHPIGASGARILVTLIGALRARGAKRGVASLCIGGGEATAMGIELI
>Mature_396_residues
TAVDQDPIVIASAARTPIAGFQGEFASLAAPQLGAAAIAAALERAGLQPEQIDEAVMGCVLPAGQGQAPARQAALGAKLP
LSVGCTTINKMCGSGMRAAMFAHDMLVAGSVDVIVAGGMESMTNAPYLLPKARAGMRMGHGQVLDHMFLDGLEDAYDKGR
LMGTFAEECAGEYAFSRDAQDAFAIESLARAKRANEDGSFAWEIAPVTVAGKKGDAVIARDEQPFKANPEKIPTLKPAFS
KTGTVTAANSSSISDGAAALVMMRASTANRLGLAPLARVVGHSTFAQAPSKFTTAPVGAIRRLFEKNGWRAAEVDLYEIN
EAFAVVTMAAMKEHGLPHEKVNVNGGACALGHPIGASGARILVTLIGALRARGAKRGVASLCIGGGEATAMGIELI

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=389, Percent_Identity=47.5578406169666, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=45.9183673469388, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=35.9897172236504, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI4501853, Length=388, Percent_Identity=36.340206185567, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=30.6413301662708, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI194353979, Length=384, Percent_Identity=28.6458333333333, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1788554, Length=389, Percent_Identity=50.1285347043702, Blast_Score=323, Evalue=9e-90,
Organism=Escherichia coli, GI87082165, Length=388, Percent_Identity=44.5876288659794, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1787663, Length=397, Percent_Identity=36.5239294710327, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI48994986, Length=403, Percent_Identity=37.2208436724566, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=28.4671532846715, Blast_Score=138, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI25147385, Length=385, Percent_Identity=41.2987012987013, Blast_Score=297, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17535921, Length=389, Percent_Identity=42.9305912596401, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=37.9487179487179, Blast_Score=267, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI17535917, Length=389, Percent_Identity=34.9614395886889, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=32.0665083135392, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=398, Percent_Identity=44.4723618090452, Blast_Score=312, Evalue=8e-86,
Organism=Saccharomyces cerevisiae, GI6322031, Length=394, Percent_Identity=31.9796954314721, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24655093, Length=389, Percent_Identity=44.987146529563, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=45.5012853470437, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI17648125, Length=395, Percent_Identity=36.2025316455696, Blast_Score=215, Evalue=5e-56,
Organism=Drosophila melanogaster, GI17137578, Length=421, Percent_Identity=31.1163895486936, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40916; Mature: 40784

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVDQDPIVIASAARTPIAGFQGEFASLAAPQLGAAAIAAALERAGLQPEQIDEAVMGC
CCCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VLPAGQGQAPARQAALGAKLPLSVGCTTINKMCGSGMRAAMFAHDMLVAGSVDVIVAGGM
CCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHEECCEEEEEECCH
ESMTNAPYLLPKARAGMRMGHGQVLDHMFLDGLEDAYDKGRLMGTFAEECAGEYAFSRDA
HHHCCCCEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCHHHCCCC
QDAFAIESLARAKRANEDGSFAWEIAPVTVAGKKGDAVIARDEQPFKANPEKIPTLKPAF
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCC
SKTGTVTAANSSSISDGAAALVMMRASTANRLGLAPLARVVGHSTFAQAPSKFTTAPVGA
CCCCEEEECCCCCCCCHHHEEEEEECCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCHHHH
IRRLFEKNGWRAAEVDLYEINEAFAVVTMAAMKEHGLPHEKVNVNGGACALGHPIGASGA
HHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCCCHH
RILVTLIGALRARGAKRGVASLCIGGGEATAMGIELI
HHHHHHHHHHHHCCHHCCHHHEEECCCCEEEEEEEEC
>Mature Secondary Structure 
TAVDQDPIVIASAARTPIAGFQGEFASLAAPQLGAAAIAAALERAGLQPEQIDEAVMGC
CCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VLPAGQGQAPARQAALGAKLPLSVGCTTINKMCGSGMRAAMFAHDMLVAGSVDVIVAGGM
CCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHEECCEEEEEECCH
ESMTNAPYLLPKARAGMRMGHGQVLDHMFLDGLEDAYDKGRLMGTFAEECAGEYAFSRDA
HHHCCCCEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCHHHCCCC
QDAFAIESLARAKRANEDGSFAWEIAPVTVAGKKGDAVIARDEQPFKANPEKIPTLKPAF
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCC
SKTGTVTAANSSSISDGAAALVMMRASTANRLGLAPLARVVGHSTFAQAPSKFTTAPVGA
CCCCEEEECCCCCCCCHHHEEEEEECCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCHHHH
IRRLFEKNGWRAAEVDLYEINEAFAVVTMAAMKEHGLPHEKVNVNGGACALGHPIGASGA
HHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCCCHH
RILVTLIGALRARGAKRGVASLCIGGGEATAMGIELI
HHHHHHHHHHHHCCHHCCHHHEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]