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The map label for this gene is gacA [H]

Identifier: 53717881

GI number: 53717881

Start: 249662

End: 250318

Strand: Reverse

Name: gacA [H]

Synonym: BPSL0239

Alternate gene names: 53717881

Gene position: 250318-249662 (Counterclockwise)

Preceding gene: 53717882

Following gene: 53717880

Centisome position: 6.14

GC content: 64.99

Gene sequence:

>657_bases
ATGAGCTTGCAGATCCTGCTCGTCGACGATCACGCGATCGTCCGCCAGGGCATCCGCCAACTGCTGATCGACCGCGGCAT
CGCGCGCGAAGTCAAGGAAGCCGAGTGCGGCGGCGATGCGCTCGTGATCGCCGAGAAAAGCGAGTTCGACGTGATCCTGC
TCGACATCTCGCTGCCCGACATGAACGGCATCGAGGTGCTCAAGCGCCTGAAGCGCAGGCTGCCGAGCACGCCGGTGCTG
ATGTTCTCGATGTACCGCGAGGATCAGTTCGCGGTGCGCGCGCTGAAGGCGGGCGCGGCGGGCTACCTGTCGAAGACCGT
GAACGCCGCGCAGATGGTGTCGGCGATCAGCCAGGTCGCCGCGGGCCGCAAGTATGTGAGCCCGGCGATGGCCGAGGCGC
TCGCCGAGTACGTGTCGTTCGAGAACGAGCCGCTGCCGCACGAGAAGCTGTCCGACCGCGAGTACCAGACGCTCTGCATG
ATCGCGTCGGGCAAGCGGCTCACCGACATCGCGCACGCGCTGTCGCTGTCGGTGAAGACGGTCAGCGTCTACCGGACGCG
CCTGCTCGAGAAGATGAAGCTGTCGAACAATGCGGAACTCACGTTCTATGTGATGAGCAACCGCCTCGTCGACATGAACC
CGGCGATCGGCGCGTAG

Upstream 100 bases:

>100_bases
CGGGCGGCGCGGGCACCGCGCTGCGCGCGCGCTTCGCGTGGCACGCGCTCATCGCCGCGCGCACGCCCGCCGCCGCGCGC
AAGGTGCGCAAGGAGGCGAG

Downstream 100 bases:

>100_bases
CGCCGCGGCCCGGCCGCTTCCCCGCTTCGGCCGCCGCGAATGCGGGGCGCGCGCCCGCCGCGGCGCCACGTCGCGCCGCC
CCGGGCGATTGATGCAAGCA

Product: response regulator protein

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSLQILLVDDHAIVRQGIRQLLIDRGIAREVKEAECGGDALVIAEKSEFDVILLDISLPDMNGIEVLKRLKRRLPSTPVL
MFSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
IASGKRLTDIAHALSLSVKTVSVYRTRLLEKMKLSNNAELTFYVMSNRLVDMNPAIGA

Sequences:

>Translated_218_residues
MSLQILLVDDHAIVRQGIRQLLIDRGIAREVKEAECGGDALVIAEKSEFDVILLDISLPDMNGIEVLKRLKRRLPSTPVL
MFSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
IASGKRLTDIAHALSLSVKTVSVYRTRLLEKMKLSNNAELTFYVMSNRLVDMNPAIGA
>Mature_217_residues
SLQILLVDDHAIVRQGIRQLLIDRGIAREVKEAECGGDALVIAEKSEFDVILLDISLPDMNGIEVLKRLKRRLPSTPVLM
FSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMI
ASGKRLTDIAHALSLSVKTVSVYRTRLLEKMKLSNNAELTFYVMSNRLVDMNPAIGA

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=36.0189573459716, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=35.8585858585859, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=33.0143540669856, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1788712, Length=209, Percent_Identity=28.7081339712919, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1787473, Length=207, Percent_Identity=34.2995169082126, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1786747, Length=212, Percent_Identity=28.3018867924528, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788546, Length=217, Percent_Identity=26.2672811059908, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=32.7731092436975, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790437, Length=107, Percent_Identity=38.3177570093458, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786599, Length=156, Percent_Identity=30.1282051282051, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24132; Mature: 24000

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQILLVDDHAIVRQGIRQLLIDRGIAREVKEAECGGDALVIAEKSEFDVILLDISLPD
CCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCC
MNGIEVLKRLKRRLPSTPVLMFSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQVA
CCHHHHHHHHHHHCCCCCEEHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMIASGKRLTDIAHALSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCCHHEEEEEECCCHHHHHHHHHHHHHHH
VSVYRTRLLEKMKLSNNAELTFYVMSNRLVDMNPAIGA
HHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCC
>Mature Secondary Structure 
SLQILLVDDHAIVRQGIRQLLIDRGIAREVKEAECGGDALVIAEKSEFDVILLDISLPD
CEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCC
MNGIEVLKRLKRRLPSTPVLMFSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQVA
CCHHHHHHHHHHHCCCCCEEHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMIASGKRLTDIAHALSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCCHHEEEEEECCCHHHHHHHHHHHHHHH
VSVYRTRLLEKMKLSNNAELTFYVMSNRLVDMNPAIGA
HHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]