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The map label for this gene is prpD [H]

Identifier: 52787772

GI number: 52787772

Start: 3919201

End: 3920640

Strand: Direct

Name: prpD [H]

Synonym: BLi04095

Alternate gene names: 52787772

Gene position: 3919201-3920640 (Clockwise)

Preceding gene: 52787771

Following gene: 52787773

Centisome position: 92.81

GC content: 51.6

Gene sequence:

>1440_bases
ATGAAAACTGAAAGTCAAACGCTCCAAAACTGTGATCAATTGCTGGAAGAGATCGCCGACTATACGGTAAATGCCAAGAT
TTCAAGCGAAGAAGCGATTGAAACCGCCCGTTACGTCTTAATGGATACACTGGGCTGCGGAATATTGGCGCTCAAATATC
CGGAATGCGCCAAGCATCTCGGCCCGATTGCCCCGGGTACCGTCGTCCCGAACGGCACCCGCGTCCCGGGAACGCAATTT
GAGCTTGATCCTGTTCACGGCGCCTTTAATATCGGCTGCATGATCCGCTGGCTCGATTATAACGACACATGGCTTGCGGC
CGAATGGGGCCATCCGTCAGATAACCTCGGAGCGATTTTAGCCTGCGCCGACTATGTCAGCAGGCAGCTGCTCGCGGCGG
GCAAGGAACCGCTCACGATTAACGATGTCCTGACGGCAATTGTTAAGGCTCATGAAATCCAAGGCGTCCTTGCTCTCGAC
AACAGCTTAAACAGAAACGGGCTTGACCATGTGCTATTCGTAAAAGTCGCCTCCACTGCTGTGGCATGCTCACTATTGGG
GGGCACAAAACAGGATGTTATCAATGCCGTCTCCCAGGCCTGGGTCGATAATTCAAGCCTGCGGACGTACCGCCATGCTC
CGAATACCGGCTCGCGTAAGTCCTGGGCAGCCGGTGATGCGACTAGCCGCGGCGTGAGGCTCGCGATGATGACCTTGAAA
GGCGAAATGGGATATAAAACCGCTTTGTCTGCTCCAATGTGGGGTTTTCAGGATGTCTTATTCGGAGGAAAAGAATTGAC
GCTCGCCAGACCGCTCGACTCTTATGTCATCGAAAATGTCCTTTTTAAAATTTCGTATCCGGCTGAATTCCACGCTCAAA
CGGCTGCTGAAGCCGCGATCAGGCTTCACCCTGAAGTGAAGGACAGACTTGATGACATCGGCCAAATCGTGATCACGACG
CACGAATCGGCCATCCGGATTATCGACAAAACGGGAGAATTACACAATCCGGCTGACCGTGATCACTGCCTTCAGTATAT
AACAGCGATCGGACTGATCTACGGCGATATTACCGCTGACCATTATGAAGACGAAACCGCGCAAAATCCGGAGATCGACA
GGCTGCGCGAAAAAATGACGACAGTCGAAAACAAACAGTATTCCGTCGATTATCTCGACCCGGAAAAACGCTCGATCGCC
AACGCTGTTCAAGTGTTTTATAAAGACGGCACACACTCGGAAAAAATCGCGGTCGAATACCCGATTGGTCATCGGCGCCG
CCGCAACGAGGGCATCCCGCTCTTAAAAGACAAATTTATCAATAATTTAAAAACGCGCTTTCCGGCGGGGCAAGCGGAAC
GAATCAACGCCCTGCTGAATGATCCTCACAGATTAAGCGGTGCACCTGTACCGGATTTCATGGAATTGTTTGTCATTTAG

Upstream 100 bases:

>100_bases
ATGAAGACAACAGGCTGTTCCGCCCGAGAGTTCACTATACGGGACCGCGTCATCTTCATCCATAATTCGCTTTGATTCAG
AAAGAAGGGAGATAGATGTT

Downstream 100 bases:

>100_bases
GAGGTTTTGAATATGTGGATTGTCTCGAAACAATCAACTCAGGAAGAGCTGGCCAATGCATTCAGAAAACTGATGAAAGC
ACCTGATATTTTGAAAATAC

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKTESQTLQNCDQLLEEIADYTVNAKISSEEAIETARYVLMDTLGCGILALKYPECAKHLGPIAPGTVVPNGTRVPGTQF
ELDPVHGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGAILACADYVSRQLLAAGKEPLTINDVLTAIVKAHEIQGVLALD
NSLNRNGLDHVLFVKVASTAVACSLLGGTKQDVINAVSQAWVDNSSLRTYRHAPNTGSRKSWAAGDATSRGVRLAMMTLK
GEMGYKTALSAPMWGFQDVLFGGKELTLARPLDSYVIENVLFKISYPAEFHAQTAAEAAIRLHPEVKDRLDDIGQIVITT
HESAIRIIDKTGELHNPADRDHCLQYITAIGLIYGDITADHYEDETAQNPEIDRLREKMTTVENKQYSVDYLDPEKRSIA
NAVQVFYKDGTHSEKIAVEYPIGHRRRRNEGIPLLKDKFINNLKTRFPAGQAERINALLNDPHRLSGAPVPDFMELFVI

Sequences:

>Translated_479_residues
MKTESQTLQNCDQLLEEIADYTVNAKISSEEAIETARYVLMDTLGCGILALKYPECAKHLGPIAPGTVVPNGTRVPGTQF
ELDPVHGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGAILACADYVSRQLLAAGKEPLTINDVLTAIVKAHEIQGVLALD
NSLNRNGLDHVLFVKVASTAVACSLLGGTKQDVINAVSQAWVDNSSLRTYRHAPNTGSRKSWAAGDATSRGVRLAMMTLK
GEMGYKTALSAPMWGFQDVLFGGKELTLARPLDSYVIENVLFKISYPAEFHAQTAAEAAIRLHPEVKDRLDDIGQIVITT
HESAIRIIDKTGELHNPADRDHCLQYITAIGLIYGDITADHYEDETAQNPEIDRLREKMTTVENKQYSVDYLDPEKRSIA
NAVQVFYKDGTHSEKIAVEYPIGHRRRRNEGIPLLKDKFINNLKTRFPAGQAERINALLNDPHRLSGAPVPDFMELFVI
>Mature_479_residues
MKTESQTLQNCDQLLEEIADYTVNAKISSEEAIETARYVLMDTLGCGILALKYPECAKHLGPIAPGTVVPNGTRVPGTQF
ELDPVHGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGAILACADYVSRQLLAAGKEPLTINDVLTAIVKAHEIQGVLALD
NSLNRNGLDHVLFVKVASTAVACSLLGGTKQDVINAVSQAWVDNSSLRTYRHAPNTGSRKSWAAGDATSRGVRLAMMTLK
GEMGYKTALSAPMWGFQDVLFGGKELTLARPLDSYVIENVLFKISYPAEFHAQTAAEAAIRLHPEVKDRLDDIGQIVITT
HESAIRIIDKTGELHNPADRDHCLQYITAIGLIYGDITADHYEDETAQNPEIDRLREKMTTVENKQYSVDYLDPEKRSIA
NAVQVFYKDGTHSEKIAVEYPIGHRRRRNEGIPLLKDKFINNLKTRFPAGQAERINALLNDPHRLSGAPVPDFMELFVI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=471, Percent_Identity=61.5711252653928, Blast_Score=611, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6325258, Length=448, Percent_Identity=54.6875, Blast_Score=494, Evalue=1e-140,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 52977; Mature: 52977

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTESQTLQNCDQLLEEIADYTVNAKISSEEAIETARYVLMDTLGCGILALKYPECAKHL
CCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHC
GPIAPGTVVPNGTRVPGTQFELDPVHGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGAIL
CCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCHHHHH
ACADYVSRQLLAAGKEPLTINDVLTAIVKAHEIQGVLALDNSLNRNGLDHVLFVKVASTA
HHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEHHHHH
VACSLLGGTKQDVINAVSQAWVDNSSLRTYRHAPNTGSRKSWAAGDATSRGVRLAMMTLK
HHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEC
GEMGYKTALSAPMWGFQDVLFGGKELTLARPLDSYVIENVLFKISYPAEFHAQTAAEAAI
CCCCCHHHHCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHHEEECCCHHHHHHHHHHHHH
RLHPEVKDRLDDIGQIVITTHESAIRIIDKTGELHNPADRDHCLQYITAIGLIYGDITAD
EECCHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
HYEDETAQNPEIDRLREKMTTVENKQYSVDYLDPEKRSIANAVQVFYKDGTHSEKIAVEY
CCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEC
PIGHRRRRNEGIPLLKDKFINNLKTRFPAGQAERINALLNDPHRLSGAPVPDFMELFVI
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKTESQTLQNCDQLLEEIADYTVNAKISSEEAIETARYVLMDTLGCGILALKYPECAKHL
CCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHC
GPIAPGTVVPNGTRVPGTQFELDPVHGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGAIL
CCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCHHHHH
ACADYVSRQLLAAGKEPLTINDVLTAIVKAHEIQGVLALDNSLNRNGLDHVLFVKVASTA
HHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEHHHHH
VACSLLGGTKQDVINAVSQAWVDNSSLRTYRHAPNTGSRKSWAAGDATSRGVRLAMMTLK
HHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEC
GEMGYKTALSAPMWGFQDVLFGGKELTLARPLDSYVIENVLFKISYPAEFHAQTAAEAAI
CCCCCHHHHCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHHEEECCCHHHHHHHHHHHHH
RLHPEVKDRLDDIGQIVITTHESAIRIIDKTGELHNPADRDHCLQYITAIGLIYGDITAD
EECCHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
HYEDETAQNPEIDRLREKMTTVENKQYSVDYLDPEKRSIANAVQVFYKDGTHSEKIAVEY
CCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEC
PIGHRRRRNEGIPLLKDKFINNLKTRFPAGQAERINALLNDPHRLSGAPVPDFMELFVI
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838 [H]