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The map label for this gene is mmgD [H]

Identifier: 52787771

GI number: 52787771

Start: 3918050

End: 3919165

Strand: Direct

Name: mmgD [H]

Synonym: BLi04094

Alternate gene names: 52787771

Gene position: 3918050-3919165 (Clockwise)

Preceding gene: 52787770

Following gene: 52787772

Centisome position: 92.79

GC content: 46.68

Gene sequence:

>1116_bases
ATGGGCAAACAAGGTGTATTTAAACCGGGATTAGAAGGCGTTATTGCTGGAGAAACGAACATTTCTTTCTTAGATACGGA
AGCGGAACAAATCGTCATTAAAGGCTATGATTTAATCGAACTTGCAGAACAAAAAACATATCTTGACCTCGTCTATCTTC
TCCTTCAAGATAAGCTGCCAGATCCAATGGAATTGAAAGGAATTGAAAGCGCTTTAAAAAGTGCGTATGAACTGCCCGGC
GGAATCTATACTATTCTGAAGTCGCTTCCGAATAATACCCATCCGATGGATGCGCTCCGAACCGGGATTTCCGCTTTAGC
AGGATATGATCCCGAATTGAATGACAGATCTCAAGCAGCAAACAGAAGCAAAGCGCTGCGCTTGATCGCACAGGTTCCGA
ATATTACCGCCAACAGCTACCGCATCCTTCACCAGAAAAACGTCATCACGCCTGACAAACAGCTTTCATTCAGCCAGAAC
TTTTTATATATGCTGACGGAAAAAGAACCGACTCCCCAAGAAACAGCCGTCTTTGATCAGACATTAATGCTTTACAGCGA
GCACGAAATGCCGAACTCCACATTTGCCGCAAGAGTCATCGCATCGACAAACACAGATATATACGGTGCTTTCACAGGCG
CTGCAGCATCGCTAAAAGGACATTTGCACGGCGGTGCGAATGAAGCGGTCATGTACATGCTGCTCGAAGGAGAAACAAAA
TCGGGCTTCTGCAAGCTGATTGAACATAAGTTAAGCAGAAAAGAAAAAATCATGGGCTTTGGGCACCGCGTCTATATGAG
AAAAATGGACCCGCGCGCAGCCTTGCTGAAAAAGGCGCTCTATTCGCTTTCTCATAAAACCGGCAGACAGGATTTATATG
AGATGTGCGATGCCGGCGAAAAGCTGATGCGCGAAGAAAAAGGCCTGTATCCGAACCTCGACTACTATGCGGCTCCAGTC
TATTACCTGCTCGGCATACCGATCGATCTCTATACTCCGATATTTTTCGCGGCACGGTCGGTCGGTCTTAGCGCGCATGT
CATTGAACAGCATGAAGACAACAGGCTGTTCCGCCCGAGAGTTCACTATACGGGACCGCGTCATCTTCATCCATAA

Upstream 100 bases:

>100_bases
AGAAACGCTCGAACGTTCATACTAATCCGCCGAACAGCCAATGCCATTCTCACAGCACGTTTTTGACACAAATGTTAAAA
AACGAATAAGAGGTGGAAAA

Downstream 100 bases:

>100_bases
TTCGCTTTGATTCAGAAAGAAGGGAGATAGATGTTATGAAAACTGAAAGTCAAACGCTCCAAAACTGTGATCAATTGCTG
GAAGAGATCGCCGACTATAC

Product: citrate synthase 3

Products: NA

Alternate protein names: Citrate synthase 3; Citrate synthase III; Methylcitrate synthase [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MGKQGVFKPGLEGVIAGETNISFLDTEAEQIVIKGYDLIELAEQKTYLDLVYLLLQDKLPDPMELKGIESALKSAYELPG
GIYTILKSLPNNTHPMDALRTGISALAGYDPELNDRSQAANRSKALRLIAQVPNITANSYRILHQKNVITPDKQLSFSQN
FLYMLTEKEPTPQETAVFDQTLMLYSEHEMPNSTFAARVIASTNTDIYGAFTGAAASLKGHLHGGANEAVMYMLLEGETK
SGFCKLIEHKLSRKEKIMGFGHRVYMRKMDPRAALLKKALYSLSHKTGRQDLYEMCDAGEKLMREEKGLYPNLDYYAAPV
YYLLGIPIDLYTPIFFAARSVGLSAHVIEQHEDNRLFRPRVHYTGPRHLHP

Sequences:

>Translated_371_residues
MGKQGVFKPGLEGVIAGETNISFLDTEAEQIVIKGYDLIELAEQKTYLDLVYLLLQDKLPDPMELKGIESALKSAYELPG
GIYTILKSLPNNTHPMDALRTGISALAGYDPELNDRSQAANRSKALRLIAQVPNITANSYRILHQKNVITPDKQLSFSQN
FLYMLTEKEPTPQETAVFDQTLMLYSEHEMPNSTFAARVIASTNTDIYGAFTGAAASLKGHLHGGANEAVMYMLLEGETK
SGFCKLIEHKLSRKEKIMGFGHRVYMRKMDPRAALLKKALYSLSHKTGRQDLYEMCDAGEKLMREEKGLYPNLDYYAAPV
YYLLGIPIDLYTPIFFAARSVGLSAHVIEQHEDNRLFRPRVHYTGPRHLHP
>Mature_370_residues
GKQGVFKPGLEGVIAGETNISFLDTEAEQIVIKGYDLIELAEQKTYLDLVYLLLQDKLPDPMELKGIESALKSAYELPGG
IYTILKSLPNNTHPMDALRTGISALAGYDPELNDRSQAANRSKALRLIAQVPNITANSYRILHQKNVITPDKQLSFSQNF
LYMLTEKEPTPQETAVFDQTLMLYSEHEMPNSTFAARVIASTNTDIYGAFTGAAASLKGHLHGGANEAVMYMLLEGETKS
GFCKLIEHKLSRKEKIMGFGHRVYMRKMDPRAALLKKALYSLSHKTGRQDLYEMCDAGEKLMREEKGLYPNLDYYAAPVY
YLLGIPIDLYTPIFFAARSVGLSAHVIEQHEDNRLFRPRVHYTGPRHLHP

Specific function: Catalyzes the synthesis of citrate from acetyl-CoA and oxaloacetate. The genomic context suggests that this protein probably functions as a 2-methylcitrate synthase, i.e. catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=371, Percent_Identity=25.3369272237197, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1786527, Length=366, Percent_Identity=36.0655737704918, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1786939, Length=383, Percent_Identity=30.0261096605744, Blast_Score=174, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17555174, Length=384, Percent_Identity=26.0416666666667, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324328, Length=327, Percent_Identity=24.1590214067278, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319850, Length=323, Percent_Identity=23.2198142414861, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640126, Length=377, Percent_Identity=25.4641909814324, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640124, Length=377, Percent_Identity=25.4641909814324, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5; =2.3.3.1 [H]

Molecular weight: Translated: 41647; Mature: 41516

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKQGVFKPGLEGVIAGETNISFLDTEAEQIVIKGYDLIELAEQKTYLDLVYLLLQDKLP
CCCCCCCCCCCCEEEECCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
DPMELKGIESALKSAYELPGGIYTILKSLPNNTHPMDALRTGISALAGYDPELNDRSQAA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
NRSKALRLIAQVPNITANSYRILHQKNVITPDKQLSFSQNFLYMLTEKEPTPQETAVFDQ
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHCCCCCCEEEEEECCCCCCHHHHHHHH
TLMLYSEHEMPNSTFAARVIASTNTDIYGAFTGAAASLKGHLHGGANEAVMYMLLEGETK
HHHHHHHCCCCCCHHHEEEEECCCCCEEEECCCHHHHHHHCCCCCCCCEEEEEEEECCCC
SGFCKLIEHKLSRKEKIMGFGHRVYMRKMDPRAALLKKALYSLSHKTGRQDLYEMCDAGE
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KLMREEKGLYPNLDYYAAPVYYLLGIPIDLYTPIFFAARSVGLSAHVIEQHEDNRLFRPR
HHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCEECCE
VHYTGPRHLHP
ECCCCCCCCCC
>Mature Secondary Structure 
GKQGVFKPGLEGVIAGETNISFLDTEAEQIVIKGYDLIELAEQKTYLDLVYLLLQDKLP
CCCCCCCCCCCEEEECCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
DPMELKGIESALKSAYELPGGIYTILKSLPNNTHPMDALRTGISALAGYDPELNDRSQAA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
NRSKALRLIAQVPNITANSYRILHQKNVITPDKQLSFSQNFLYMLTEKEPTPQETAVFDQ
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHCCCCCCEEEEEECCCCCCHHHHHHHH
TLMLYSEHEMPNSTFAARVIASTNTDIYGAFTGAAASLKGHLHGGANEAVMYMLLEGETK
HHHHHHHCCCCCCHHHEEEEECCCCCEEEECCCHHHHHHHCCCCCCCCEEEEEEEECCCC
SGFCKLIEHKLSRKEKIMGFGHRVYMRKMDPRAALLKKALYSLSHKTGRQDLYEMCDAGE
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KLMREEKGLYPNLDYYAAPVYYLLGIPIDLYTPIFFAARSVGLSAHVIEQHEDNRLFRPR
HHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCEECCE
VHYTGPRHLHP
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759838; 8969508; 9384377 [H]