Click here to switch to the map view.

The map label for this gene is thiE

Identifier: 52787728

GI number: 52787728

Start: 3877504

End: 3878178

Strand: Reverse

Name: thiE

Synonym: BLi04051

Alternate gene names: 52787728

Gene position: 3878178-3877504 (Counterclockwise)

Preceding gene: 52787729

Following gene: 52787725

Centisome position: 91.84

GC content: 52.3

Gene sequence:

>675_bases
ATGACGCGAGTCTCAGAAGAAGCAATGAAAGATCTGTTGTCGGTCTATTTTATTATGGGGTCAAACAATACCGCAGGGGA
TCCTTTAACTGTTATTGAAAAAGCTTTAAAAGGCGGTGCGACCCTTTTTCAATTCCGCGAAAAAGGCGAGGGTGCGTTGA
AAGCCGGAGATCAAACGGCGTTTGCCCGACAGGTGCAGGCGCTGTGCAAACAGTTCAATGTACCGTTTATTATCAACGAT
GATGTAGAACTCGCGCTTGAACTTGACGCAGATGGCGTGCATATCGGCCAGGATGATGATAAGGCCGCAGACGTCAGAGC
GAGAATCGGGGACAAAATCCTCGGCGTTTCCGCGCATACACTTGAAGAAGTCTTAAAGGCGGAAAAGGATGGAGCGGATT
ACATCGGTGCGGGGCCTGTTTACCCTACTGAAACAAAGCGGGATACAAAAGCTGTGCAGGGCGTCTCGCTCATTCAAGAG
ATCCGCAGGCAGGGAATTGGCATTCCTGTGGTCGGAATCGGCGGGATCACGGTCGAAAACTGCGTCCCCGTCATCGAGGC
CGGGGCGGACGGCATCAGTGTCATCAGCGCCATCAGCAAAGCCGCCGATCCAAAGCAGGCCGCCGAGGCGTTTAGCGAGA
AAGTCCAAGCTACCAAACAAAGCGCACATTCCTAA

Upstream 100 bases:

>100_bases
CGGGGGCCCGGAAGTTTCCAAATCGAGTTTTTGAATCAGCTTGCTGCCGTTGAAGCTATGGATATAAAAGAACGCGGCAG
GATCAGAGAGGTGGAATAAG

Downstream 100 bases:

>100_bases
ATGGAATATGCGCTTTTCAGCGTGTCGAGAAACCCTCGCATTCGTTGTCAGGCCTGCGCGTCGGTGCTCACGAATTCCAA
CATTCGCTCCGCTCCGATGC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTRVSEEAMKDLLSVYFIMGSNNTAGDPLTVIEKALKGGATLFQFREKGEGALKAGDQTAFARQVQALCKQFNVPFIIND
DVELALELDADGVHIGQDDDKAADVRARIGDKILGVSAHTLEEVLKAEKDGADYIGAGPVYPTETKRDTKAVQGVSLIQE
IRRQGIGIPVVGIGGITVENCVPVIEAGADGISVISAISKAADPKQAAEAFSEKVQATKQSAHS

Sequences:

>Translated_224_residues
MTRVSEEAMKDLLSVYFIMGSNNTAGDPLTVIEKALKGGATLFQFREKGEGALKAGDQTAFARQVQALCKQFNVPFIIND
DVELALELDADGVHIGQDDDKAADVRARIGDKILGVSAHTLEEVLKAEKDGADYIGAGPVYPTETKRDTKAVQGVSLIQE
IRRQGIGIPVVGIGGITVENCVPVIEAGADGISVISAISKAADPKQAAEAFSEKVQATKQSAHS
>Mature_223_residues
TRVSEEAMKDLLSVYFIMGSNNTAGDPLTVIEKALKGGATLFQFREKGEGALKAGDQTAFARQVQALCKQFNVPFIINDD
VELALELDADGVHIGQDDDKAADVRARIGDKILGVSAHTLEEVLKAEKDGADYIGAGPVYPTETKRDTKAVQGVSLIQEI
RRQGIGIPVVGIGGITVENCVPVIEAGADGISVISAISKAADPKQAAEAFSEKVQATKQSAHS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=190, Percent_Identity=35.2631578947368, Blast_Score=88, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6325042, Length=208, Percent_Identity=37.0192307692308, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_BACLD (Q65DK0)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_081129.1
- RefSeq:   YP_093557.1
- HSSP:   P39594
- ProteinModelPortal:   Q65DK0
- SMR:   Q65DK0
- STRING:   Q65DK0
- EnsemblBacteria:   EBBACT00000054209
- EnsemblBacteria:   EBBACT00000061552
- GeneID:   3030322
- GeneID:   3097679
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi04051
- KEGG:   bli:BL03866
- NMPDR:   fig|279010.5.peg.3172
- eggNOG:   COG0352
- GeneTree:   EBGT00050000001882
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   PRK00043
- BioCyc:   BLIC279010-1:BLI04051-MONOMER
- BioCyc:   BLIC279010:BL03866-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23650; Mature: 23519

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVSEEAMKDLLSVYFIMGSNNTAGDPLTVIEKALKGGATLFQFREKGEGALKAGDQTA
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHH
FARQVQALCKQFNVPFIINDDVELALELDADGVHIGQDDDKAADVRARIGDKILGVSAHT
HHHHHHHHHHHCCCCEEECCCCEEEEEECCCCEECCCCCCHHHHHHHHHCCHHHCCCHHH
LEEVLKAEKDGADYIGAGPVYPTETKRDTKAVQGVSLIQEIRRQGIGIPVVGIGGITVEN
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEHHH
CVPVIEAGADGISVISAISKAADPKQAAEAFSEKVQATKQSAHS
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRVSEEAMKDLLSVYFIMGSNNTAGDPLTVIEKALKGGATLFQFREKGEGALKAGDQTA
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHH
FARQVQALCKQFNVPFIINDDVELALELDADGVHIGQDDDKAADVRARIGDKILGVSAHT
HHHHHHHHHHHCCCCEEECCCCEEEEEECCCCEECCCCCCHHHHHHHHHCCHHHCCCHHH
LEEVLKAEKDGADYIGAGPVYPTETKRDTKAVQGVSLIQEIRRQGIGIPVVGIGGITVEN
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEHHH
CVPVIEAGADGISVISAISKAADPKQAAEAFSEKVQATKQSAHS
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA