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The map label for this gene is thiM

Identifier: 52787729

GI number: 52787729

Start: 3878180

End: 3878989

Strand: Reverse

Name: thiM

Synonym: BLi04052

Alternate gene names: 52787729

Gene position: 3878989-3878180 (Counterclockwise)

Preceding gene: 52787730

Following gene: 52787728

Centisome position: 91.86

GC content: 53.09

Gene sequence:

>810_bases
ATGAATATGGAAGATGCCGCAAGGGGAATTGAGTTAGTCAGAAAGCAAAAACCGCTCGTACACAATATGACAAACAATGT
CGTCACCAACTTTACGGCAAACGGTTTGCTGGCTCTCGGCGCATCGCCGGTGATGGCGTATGCGAGGGAAGAGGCCGCCG
ACATGGCAAAGATCGCCGGAGCCCTTGTGCTGAACATCGGCACGCTCAGCCGTGAATCTGTTGAAGCGATGATTATTGCA
GGAAAGTCAGCTAATGAACATGGGGTGCCGGTTATTTTTGATCCGGTCGGTGCGGGGGCGACGCCGTTTCGAACGGAATC
GGCTCAAGCGATCATGCGCAAAGTGAAGGTTTCTGCCGTCAGAGGCAATGCTGCGGAAATTGCCAACACGCTTGGGGAAG
ACTGGCTGATCAAAGGCGTGGACGCGGGTGAAGAGAGCGGCGACAGAACGGAGCTCGCAAAAAAAGCCGCGAAATTATGG
GATACAGCAGTCATCATAACCGGTGCTGAAGACGTGATAACGGATGGTACAAAAACGTATACCGTGGGCAACGGACATCA
GCTGCTTACGAAAGTGACGGGGACAGGCTGCTTGTTTACCTCTGTCATCGGTGCGTTTTGCGCCGTTGAAAAAGACGTTT
GCCGTGCCGCTGTATCTGCAGCCGCTTTTTATGGAACAGCGGCCGAGTTGGCCGCGGCGAAAACGGAAAGCCGGGGGCCC
GGAAGTTTCCAAATCGAGTTTTTGAATCAGCTTGCTGCCGTTGAAGCTATGGATATAAAAGAACGCGGCAGGATCAGAGA
GGTGGAATAA

Upstream 100 bases:

>100_bases
GAAAAGACCGCTATCTGTCGTTCGCGGCGAGGGCGTGGATTGATCATACAAAATCGTATATATGGAGCGAGAATTCAAAA
ACAGAAAGGAATGCAGGCGA

Downstream 100 bases:

>100_bases
GATGACGCGAGTCTCAGAAGAAGCAATGAAAGATCTGTTGTCGGTCTATTTTATTATGGGGTCAAACAATACCGCAGGGG
ATCCTTTAACTGTTATTGAA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNMEDAARGIELVRKQKPLVHNMTNNVVTNFTANGLLALGASPVMAYAREEAADMAKIAGALVLNIGTLSRESVEAMIIA
GKSANEHGVPVIFDPVGAGATPFRTESAQAIMRKVKVSAVRGNAAEIANTLGEDWLIKGVDAGEESGDRTELAKKAAKLW
DTAVIITGAEDVITDGTKTYTVGNGHQLLTKVTGTGCLFTSVIGAFCAVEKDVCRAAVSAAAFYGTAAELAAAKTESRGP
GSFQIEFLNQLAAVEAMDIKERGRIREVE

Sequences:

>Translated_269_residues
MNMEDAARGIELVRKQKPLVHNMTNNVVTNFTANGLLALGASPVMAYAREEAADMAKIAGALVLNIGTLSRESVEAMIIA
GKSANEHGVPVIFDPVGAGATPFRTESAQAIMRKVKVSAVRGNAAEIANTLGEDWLIKGVDAGEESGDRTELAKKAAKLW
DTAVIITGAEDVITDGTKTYTVGNGHQLLTKVTGTGCLFTSVIGAFCAVEKDVCRAAVSAAAFYGTAAELAAAKTESRGP
GSFQIEFLNQLAAVEAMDIKERGRIREVE
>Mature_269_residues
MNMEDAARGIELVRKQKPLVHNMTNNVVTNFTANGLLALGASPVMAYAREEAADMAKIAGALVLNIGTLSRESVEAMIIA
GKSANEHGVPVIFDPVGAGATPFRTESAQAIMRKVKVSAVRGNAAEIANTLGEDWLIKGVDAGEESGDRTELAKKAAKLW
DTAVIITGAEDVITDGTKTYTVGNGHQLLTKVTGTGCLFTSVIGAFCAVEKDVCRAAVSAAAFYGTAAELAAAKTESRGP
GSFQIEFLNQLAAVEAMDIKERGRIREVE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=247, Percent_Identity=39.2712550607287, Blast_Score=162, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325042, Length=270, Percent_Identity=29.6296296296296, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_BACLD (Q65DJ9)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_081130.1
- RefSeq:   YP_093558.1
- HSSP:   P39593
- ProteinModelPortal:   Q65DJ9
- SMR:   Q65DJ9
- STRING:   Q65DJ9
- EnsemblBacteria:   EBBACT00000056026
- EnsemblBacteria:   EBBACT00000060581
- GeneID:   3030181
- GeneID:   3099156
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi04052
- KEGG:   bli:BL03865
- NMPDR:   fig|279010.5.peg.3173
- eggNOG:   COG2145
- GeneTree:   EBGT00050000002080
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   BLIC279010-1:BLI04052-MONOMER
- BioCyc:   BLIC279010:BL03865-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28241; Mature: 28241

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: BINDING 45-45 BINDING 121-121 BINDING 167-167 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMEDAARGIELVRKQKPLVHNMTNNVVTNFTANGLLALGASPVMAYAREEAADMAKIAG
CCHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
ALVLNIGTLSRESVEAMIIAGKSANEHGVPVIFDPVGAGATPFRTESAQAIMRKVKVSAV
HHHEECCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
RGNAAEIANTLGEDWLIKGVDAGEESGDRTELAKKAAKLWDTAVIITGAEDVITDGTKTY
CCCHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHCCCCEEE
TVGNGHQLLTKVTGTGCLFTSVIGAFCAVEKDVCRAAVSAAAFYGTAAELAAAKTESRGP
EECCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GSFQIEFLNQLAAVEAMDIKERGRIREVE
CCEEHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNMEDAARGIELVRKQKPLVHNMTNNVVTNFTANGLLALGASPVMAYAREEAADMAKIAG
CCHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
ALVLNIGTLSRESVEAMIIAGKSANEHGVPVIFDPVGAGATPFRTESAQAIMRKVKVSAV
HHHEECCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
RGNAAEIANTLGEDWLIKGVDAGEESGDRTELAKKAAKLWDTAVIITGAEDVITDGTKTY
CCCHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHCCCCEEE
TVGNGHQLLTKVTGTGCLFTSVIGAFCAVEKDVCRAAVSAAAFYGTAAELAAAKTESRGP
EECCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GSFQIEFLNQLAAVEAMDIKERGRIREVE
CCEEHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA