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The map label for this gene is thiD [H]

Identifier: 52787691

GI number: 52787691

Start: 3837137

End: 3837949

Strand: Direct

Name: thiD [H]

Synonym: BLi04014

Alternate gene names: 52787691

Gene position: 3837137-3837949 (Clockwise)

Preceding gene: 52787688

Following gene: 52787696

Centisome position: 90.87

GC content: 50.68

Gene sequence:

>813_bases
ATGACAATGCACAAAGCACTGACAATCGCCGGCTCTGACTCAAGCGGAGGCGCCGGCATTCAGGCGGACTTAAAAACGTT
CCAGGAAAAGAACGTCTATGGAATGACGGCCCTTACGGTTGTTGTCGCCATGGACCCTGAGAACAATTGGAATCATCAGG
TGTTCCCAGTTGATACGGAAGTCATCCGCGCTCAGCTGTCGACGATTGTGGACGGCATCGGTGTAGATGCGATGAAAACG
GGCATGCTTCCGACAGTTGACATAATTGAATTGGCGGCAGACACAATTAAGAAGCACAGCATGAAAAATGTCGTCATTGA
TCCGGTTATGGTATGCAAAGGGGCAAATGAAGTGCTGTATCCCGAGCACGCAAAGGCTTTAAGAGAGCTTTTGGCTCCGC
TTGCCACGGTCATTACGCCAAATCTTTTTGAAGCCGGCCAGCTCAGCCGCCTTGGAGAAATTAAAACGGTTGAGCAAATG
AAGGAAGCGGCAAAGGCCATCCACGGCCTCGGCGCCCAACACGTCCTCATTACAGGGGGAGGCAAACTAAATCACGAAAA
AGCGGTAGATGTATTATTTGACGGCCGGGATGCAGAGGTGATCGAAGGAGAAAGAATCGACACCCCATACACGCACGGGG
CGGGCTGCACCTACTCCGCGGCAATTACGGCTGAGCTTGCCAAAGGTTCAAATGTAAAAGAAGCCATTTATTCTGCAAAA
GCGTTTATTACGGAAGCGATTCGCGAATCGTTCCGTTTGAATCAATACGTCGGGCCGACAAAGCACTCCGCTTTACGACT
CAGCGGCAAATAA

Upstream 100 bases:

>100_bases
AAAGCGGCAGCCATTTCATTGTGATCTTGCGAAAAGCTGTCTATAATAAGTAATAAACCAACTTTGGATGGAATCTCTTA
TCGTGGAGGATTGACTGAAC

Downstream 100 bases:

>100_bases
AAAAAGCCCCTTTTATGTAAAAGGGGCTTTCATTTAGCTTTCCCGTTTGATCGTTCTTGCAGATGATCTTAAGAAAGATT
CGATCGATTCCACAAAAAGA

Product: pyridoxal kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVVVAMDPENNWNHQVFPVDTEVIRAQLSTIVDGIGVDAMKT
GMLPTVDIIELAADTIKKHSMKNVVIDPVMVCKGANEVLYPEHAKALRELLAPLATVITPNLFEAGQLSRLGEIKTVEQM
KEAAKAIHGLGAQHVLITGGGKLNHEKAVDVLFDGRDAEVIEGERIDTPYTHGAGCTYSAAITAELAKGSNVKEAIYSAK
AFITEAIRESFRLNQYVGPTKHSALRLSGK

Sequences:

>Translated_270_residues
MTMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVVVAMDPENNWNHQVFPVDTEVIRAQLSTIVDGIGVDAMKT
GMLPTVDIIELAADTIKKHSMKNVVIDPVMVCKGANEVLYPEHAKALRELLAPLATVITPNLFEAGQLSRLGEIKTVEQM
KEAAKAIHGLGAQHVLITGGGKLNHEKAVDVLFDGRDAEVIEGERIDTPYTHGAGCTYSAAITAELAKGSNVKEAIYSAK
AFITEAIRESFRLNQYVGPTKHSALRLSGK
>Mature_269_residues
TMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVVVAMDPENNWNHQVFPVDTEVIRAQLSTIVDGIGVDAMKTG
MLPTVDIIELAADTIKKHSMKNVVIDPVMVCKGANEVLYPEHAKALRELLAPLATVITPNLFEAGQLSRLGEIKTVEQMK
EAAKAIHGLGAQHVLITGGGKLNHEKAVDVLFDGRDAEVIEGERIDTPYTHGAGCTYSAAITAELAKGSNVKEAIYSAKA
FITEAIRESFRLNQYVGPTKHSALRLSGK

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates. Can also use hydroxymethylpyrimidine (HMP) as substrate [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=260, Percent_Identity=35.3846153846154, Blast_Score=167, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325378, Length=277, Percent_Identity=31.0469314079422, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=29.9270072992701, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324517, Length=275, Percent_Identity=30.5454545454545, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 28936; Mature: 28804

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVVVAMDPENNWNHQVFPVDTE
CCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEEECCCHH
VIRAQLSTIVDGIGVDAMKTGMLPTVDIIELAADTIKKHSMKNVVIDPVMVCKGANEVLY
HHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHCCHHHEECCCCCCCC
PEHAKALRELLAPLATVITPNLFEAGQLSRLGEIKTVEQMKEAAKAIHGLGAQHVLITGG
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
GKLNHEKAVDVLFDGRDAEVIEGERIDTPYTHGAGCTYSAAITAELAKGSNVKEAIYSAK
CCCCCHHHEEEEECCCCCCEECCCCCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHH
AFITEAIRESFRLNQYVGPTKHSALRLSGK
HHHHHHHHHHHHHHHCCCCCCCCEEEECCC
>Mature Secondary Structure 
TMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVVVAMDPENNWNHQVFPVDTE
CCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEEECCCHH
VIRAQLSTIVDGIGVDAMKTGMLPTVDIIELAADTIKKHSMKNVVIDPVMVCKGANEVLY
HHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHCCHHHEECCCCCCCC
PEHAKALRELLAPLATVITPNLFEAGQLSRLGEIKTVEQMKEAAKAIHGLGAQHVLITGG
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
GKLNHEKAVDVLFDGRDAEVIEGERIDTPYTHGAGCTYSAAITAELAKGSNVKEAIYSAK
CCCCCHHHEEEEECCCCCCEECCCCCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHH
AFITEAIRESFRLNQYVGPTKHSALRLSGK
HHHHHHHHHHHHHHHCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]