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The map label for this gene is prfA

Identifier: 52787627

GI number: 52787627

Start: 3773499

End: 3774569

Strand: Reverse

Name: prfA

Synonym: BLi03949

Alternate gene names: 52787627

Gene position: 3774569-3773499 (Counterclockwise)

Preceding gene: 52787630

Following gene: 52787626

Centisome position: 89.39

GC content: 49.86

Gene sequence:

>1071_bases
GTGTTAGACCGTTTAAAATCGATAGAGGACCGGTACGAAAAATTAAATGAACTATTAAGCGATCCGGAGGTTGTCAACGA
TCCGAAGAAGCTTAGAGAATATTCGAAAGAACAATCTGATTTACAGGAAACAGTTGAAGTATACAGACGTTATCGTAGCG
CGTCGGAACAGCTGAGCGATGCTAAAGCGATGCTTGAAGAAAAGCTTGACTCCGACATGCGCGAGATGGTGAAAGAGGAG
ATTTCCGAACTGCAGGAAGAAATCGAGTCGCTCACAGATCAGCTGAAGGTATTGCTGATCCCTAAAGATCCGAATGATGA
CAAAAACGTTATCATGGAAATCCGCGGCGCAGCGGGCGGAGAAGAGGCCGCTTTATTTGCCGGCAACCTTTACCGCATGT
ACAGCCGCTACGCAGAGCTCCAAGGCTGGAAAACAGAAGTGATGGAAGCGAATATGACAGGTACAGGCGGATATAAAGAG
ATCATTTTTATGATCAATGGGAACGGCGCATACTCAAGGCTGAAGTATGAGAACGGCGCACACCGCGTACAGCGCGTTCC
AGAGACCGAATCCGGCGGGCGCATCCATACATCCACGGCAACGGTAGCCTGCCTGCCTGAAGCGGAAGAGGTAGAAGTCG
ATATACATGAAAAAGACATTCGCGTCGACACCTTCGCATCGAGCGGTCCGGGAGGGCAAAGCGTCAATACGACGATGTCA
GCCGTCCGTCTGACCCACCTTCCGACAGGTGTTGTCGTGTCTTGCCAGGATGAGAAATCTCAGATCAAAAACAAAGAAAA
AGCGATGAAGGTTCTGCGCGCGCGAATTTACGACAAATTTCAGCAGGAAGCTCAGGCTGAATATGATCAAAACCGGAAAT
CCGCCGTCGGAACAGGAGACCGCTCTGAGCGCATCCGCACGTATAACTTTCCGCAAAACCGCGTTACCGATCACAGGATC
GGCTTGACGATCCAAAAGCTCGACCAAATCCTTGAAGGCAAGCTTGATGAAGTGATTGACGCCCTGATTGTAGAGGATCA
GGCAAGCAAGCTTCAGCAGGCGGAGAGCTAA

Upstream 100 bases:

>100_bases
AAAGACGCGGCTGCTGCCGATAACGGCGAAGTTGTTTTGACGGGGAAGAGTCCCTATACTATAATTAGAATGTTACGCAC
AGCAAACAGAGGTGAATAGC

Downstream 100 bases:

>100_bases
ACATGAAAACAATTTTTGAAGCCCTCAGGTGGGCTTCTTCTTATTTGACGGAAGCGGGAAGAGACCGGAATGCCGCGGAA
ATTTTGCTGACAGATCAGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLDRLKSIEDRYEKLNELLSDPEVVNDPKKLREYSKEQSDLQETVEVYRRYRSASEQLSDAKAMLEEKLDSDMREMVKEE
ISELQEEIESLTDQLKVLLIPKDPNDDKNVIMEIRGAAGGEEAALFAGNLYRMYSRYAELQGWKTEVMEANMTGTGGYKE
IIFMINGNGAYSRLKYENGAHRVQRVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQNRKSAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILEGKLDEVIDALIVEDQASKLQQAES

Sequences:

>Translated_356_residues
MLDRLKSIEDRYEKLNELLSDPEVVNDPKKLREYSKEQSDLQETVEVYRRYRSASEQLSDAKAMLEEKLDSDMREMVKEE
ISELQEEIESLTDQLKVLLIPKDPNDDKNVIMEIRGAAGGEEAALFAGNLYRMYSRYAELQGWKTEVMEANMTGTGGYKE
IIFMINGNGAYSRLKYENGAHRVQRVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQNRKSAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILEGKLDEVIDALIVEDQASKLQQAES
>Mature_356_residues
MLDRLKSIEDRYEKLNELLSDPEVVNDPKKLREYSKEQSDLQETVEVYRRYRSASEQLSDAKAMLEEKLDSDMREMVKEE
ISELQEEIESLTDQLKVLLIPKDPNDDKNVIMEIRGAAGGEEAALFAGNLYRMYSRYAELQGWKTEVMEANMTGTGGYKE
IIFMINGNGAYSRLKYENGAHRVQRVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQNRKSAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILEGKLDEVIDALIVEDQASKLQQAES

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=275, Percent_Identity=46.9090909090909, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=36.3896848137536, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI166795305, Length=180, Percent_Identity=45, Blast_Score=173, Evalue=2e-43,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=50.8522727272727, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=35.8208955223881, Blast_Score=211, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=330, Percent_Identity=34.2424242424242, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=327, Percent_Identity=43.4250764525994, Blast_Score=238, Evalue=9e-64,
Organism=Drosophila melanogaster, GI19921226, Length=334, Percent_Identity=40.7185628742515, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_BACLD (Q65DV1)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_081026.1
- RefSeq:   YP_093456.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q65DV1
- SMR:   Q65DV1
- STRING:   Q65DV1
- EnsemblBacteria:   EBBACT00000055230
- EnsemblBacteria:   EBBACT00000058637
- GeneID:   3028131
- GeneID:   3101324
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03949
- KEGG:   bli:BL03978
- NMPDR:   fig|279010.5.peg.4086
- eggNOG:   COG0216
- GeneTree:   EBGT00050000000673
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   BLIC279010-1:BLI03949-MONOMER
- BioCyc:   BLIC279010:BL03978-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR012347
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- Gene3D:   G3DSA:1.20.1260.10
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40378; Mature: 40378

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLKSIEDRYEKLNELLSDPEVVNDPKKLREYSKEQSDLQETVEVYRRYRSASEQLSD
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAMLEEKLDSDMREMVKEEISELQEEIESLTDQLKVLLIPKDPNDDKNVIMEIRGAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAGNLYRMYSRYAELQGWKTEVMEANMTGTGGYKEIIFMINGNGAYSRLKYENGA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCEEEECCCH
HRVQRVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHH
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQNRKSAVGTGD
HHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDQILEGKLDEVIDALIVEDQASKLQQAES
CCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDRLKSIEDRYEKLNELLSDPEVVNDPKKLREYSKEQSDLQETVEVYRRYRSASEQLSD
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAMLEEKLDSDMREMVKEEISELQEEIESLTDQLKVLLIPKDPNDDKNVIMEIRGAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAGNLYRMYSRYAELQGWKTEVMEANMTGTGGYKEIIFMINGNGAYSRLKYENGA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCEEEECCCH
HRVQRVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHH
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQNRKSAVGTGD
HHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDQILEGKLDEVIDALIVEDQASKLQQAES
CCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA