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The map label for this gene is ywkE [H]

Identifier: 52787626

GI number: 52787626

Start: 3772630

End: 3773496

Strand: Reverse

Name: ywkE [H]

Synonym: BLi03948

Alternate gene names: 52787626

Gene position: 3773496-3772630 (Counterclockwise)

Preceding gene: 52787627

Following gene: 52787624

Centisome position: 89.36

GC content: 48.56

Gene sequence:

>867_bases
ATGAAAACAATTTTTGAAGCCCTCAGGTGGGCTTCTTCTTATTTGACGGAAGCGGGAAGAGACCGGAATGCCGCGGAAAT
TTTGCTGACAGATCAGCTGAACATCGACCGCAGCAAACTGTTGGCAAGCTTCCATGATGTCATATCAGAGTCTGATTTTG
CGAGGTTTAAAAAATCTGTCGAGCTTCATCATCAAGGAGTGCCTGTTCAATATATCACCGGAAAAGAGTCCTTTTACGGA
AGGGAATTTTCGGTAAATGAACACGTTCTGATTCCGCGTCCTGAAACAGAGGAAGTCGTTGAAGCTGTCCTTTCCGAAGC
GGAAAGAGTTTTTCACGGGACAGATCGTCTTAAAGCTGTCGATGTCGGTACAGGAAGCGGGGCGATTGCAGTGACCCTTG
CGCTTGAGAGCCCGCGTTTTTCCGTCACGGCGACGGATATTTCGGAACAAGCTTTAAGTACCGCAAGACACAACGCTGAC
CGGCTTGGGGCGAAAGTGGATTTCCTCTGCGGTGATTTGCTTGAACCGCTTATCGCGCGACAGAAGAAAGCTGATGTCAT
CGTTTCGAATCCACCTTATATATCCGAGGAAGACATGAGGACGCTGTCAGATGTCGTCCGTCTCCATGAACCCGCCGGGG
CTTTAACGGACGGTGCTGACGGATTGCAGTTTTATAAGCGTTTCATGAAGGAAATTCCATCTGTCATCGCAGATCAGGCA
CTGGTGGTGTTTGAAATCGGCTGGACTCAGGGGAATGCCGTTAAAGACATGTTTCACCGGTTCTTTCCTGATGCTGACGT
GCGGGTGAAGAAAGATCTTAATGGAAAAGACCGCATCGTCTGCGCGGTAATTGAACAACATAGTTAA

Upstream 100 bases:

>100_bases
AAAAGCTCGACCAAATCCTTGAAGGCAAGCTTGATGAAGTGATTGACGCCCTGATTGTAGAGGATCAGGCAAGCAAGCTT
CAGCAGGCGGAGAGCTAAAC

Downstream 100 bases:

>100_bases
AAGTGACTCCAAAAGTTAGACAATAAAATGTCATGAAACAGTTTAACTGGCATGATTCCGGTATTGTACCAGGCTCATGC
CTTTTCATTTGATCTTGATC

Product: YwkE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKTIFEALRWASSYLTEAGRDRNAAEILLTDQLNIDRSKLLASFHDVISESDFARFKKSVELHHQGVPVQYITGKESFYG
REFSVNEHVLIPRPETEEVVEAVLSEAERVFHGTDRLKAVDVGTGSGAIAVTLALESPRFSVTATDISEQALSTARHNAD
RLGAKVDFLCGDLLEPLIARQKKADVIVSNPPYISEEDMRTLSDVVRLHEPAGALTDGADGLQFYKRFMKEIPSVIADQA
LVVFEIGWTQGNAVKDMFHRFFPDADVRVKKDLNGKDRIVCAVIEQHS

Sequences:

>Translated_288_residues
MKTIFEALRWASSYLTEAGRDRNAAEILLTDQLNIDRSKLLASFHDVISESDFARFKKSVELHHQGVPVQYITGKESFYG
REFSVNEHVLIPRPETEEVVEAVLSEAERVFHGTDRLKAVDVGTGSGAIAVTLALESPRFSVTATDISEQALSTARHNAD
RLGAKVDFLCGDLLEPLIARQKKADVIVSNPPYISEEDMRTLSDVVRLHEPAGALTDGADGLQFYKRFMKEIPSVIADQA
LVVFEIGWTQGNAVKDMFHRFFPDADVRVKKDLNGKDRIVCAVIEQHS
>Mature_288_residues
MKTIFEALRWASSYLTEAGRDRNAAEILLTDQLNIDRSKLLASFHDVISESDFARFKKSVELHHQGVPVQYITGKESFYG
REFSVNEHVLIPRPETEEVVEAVLSEAERVFHGTDRLKAVDVGTGSGAIAVTLALESPRFSVTATDISEQALSTARHNAD
RLGAKVDFLCGDLLEPLIARQKKADVIVSNPPYISEEDMRTLSDVVRLHEPAGALTDGADGLQFYKRFMKEIPSVIADQA
LVVFEIGWTQGNAVKDMFHRFFPDADVRVKKDLNGKDRIVCAVIEQHS

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=234, Percent_Identity=31.6239316239316, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787463, Length=252, Percent_Identity=31.7460317460317, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87082085, Length=303, Percent_Identity=29.3729372937294, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324266, Length=217, Percent_Identity=29.0322580645161, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24582226, Length=199, Percent_Identity=31.6582914572864, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32071; Mature: 32071

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIFEALRWASSYLTEAGRDRNAAEILLTDQLNIDRSKLLASFHDVISESDFARFKKSV
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
ELHHQGVPVQYITGKESFYGREFSVNEHVLIPRPETEEVVEAVLSEAERVFHGTDRLKAV
HHHCCCCCEEEEECCHHHCCCEECCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEE
DVGTGSGAIAVTLALESPRFSVTATDISEQALSTARHNADRLGAKVDFLCGDLLEPLIAR
ECCCCCCEEEEEEEECCCCEEEEECHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHH
QKKADVIVSNPPYISEEDMRTLSDVVRLHEPAGALTDGADGLQFYKRFMKEIPSVIADQA
HHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCE
LVVFEIGWTQGNAVKDMFHRFFPDADVRVKKDLNGKDRIVCAVIEQHS
EEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKTIFEALRWASSYLTEAGRDRNAAEILLTDQLNIDRSKLLASFHDVISESDFARFKKSV
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
ELHHQGVPVQYITGKESFYGREFSVNEHVLIPRPETEEVVEAVLSEAERVFHGTDRLKAV
HHHCCCCCEEEEECCHHHCCCEECCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEE
DVGTGSGAIAVTLALESPRFSVTATDISEQALSTARHNADRLGAKVDFLCGDLLEPLIAR
ECCCCCCEEEEEEEECCCCEEEEECHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHH
QKKADVIVSNPPYISEEDMRTLSDVVRLHEPAGALTDGADGLQFYKRFMKEIPSVIADQA
HHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCE
LVVFEIGWTQGNAVKDMFHRFFPDADVRVKKDLNGKDRIVCAVIEQHS
EEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]