Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 52787169

GI number: 52787169

Start: 3319143

End: 3320297

Strand: Reverse

Name: dacB [H]

Synonym: BLi03478

Alternate gene names: 52787169

Gene position: 3320297-3319143 (Counterclockwise)

Preceding gene: 52787172

Following gene: 52787168

Centisome position: 78.63

GC content: 46.15

Gene sequence:

>1155_bases
ATGAATGTTAAACGATCCCTCCTGTTCATTATTGTACTATGTCTGACGCTGTTTTTTCATAGCTCGGCTCAAGCAGGAGA
AAAAAACAAACCGGAAATCAAAAGCGTTTCCGCTATATTGATCGATGGGAAATCGGGCCAGGTCCTCTATGAAAAAAACA
GCCGCAAAAAAATGTATCCAGCCAGCATAACCAAAATTGCCACGGCGATTTATGCAATAGAAAACGGAAATTTGGAAGAT
CGTGTGAAAGTCAGCAAAAATGCCGCTCAAACGGAAGGATCAAGCGTATATCTGGAAGAGGGGGAGTCGGTTCCATTAAA
ACGGCTGCTCCAAGGGCTGCTGATGAATTCGGGAAATGACGCCGGTACGGCGATCGCCGAACATCTCAGCGGAAGCGTGA
AACAGTTTGCGGATGATTTGAACGCGTTTATTGCCGAACATGCCGGAGTGCATGACACTCATTTTATGAATCCTCACGGG
CTGTTTGACAAAAATCATTATACGACGTCTGCCGACATGGCGAGAATTACTGAGTACGCAATGAAAAACAAAACGTTCCG
AGCGCTTTTTTCGGAAAAGCAAACGGATTGGCACGGACAGACATGGGACACGACGCTTAAAAACCATCATCGAATGCTGA
CGGGTGAGATTCCTTACAAAGGTATAACCGGCGGCAAAACAGGATTTGTGGATGAATCAAAGCATACCCTTGTCACGACG
GCAACGCGGGGCAGCCTTGATTTAATTGCGGTCGTCATGAAGGCAGATAGCAAGAAAATGATGTACAGCGATACAAAAGC
TCTTCTGGATGAGGGATTTCACAATTTCGAAACACAAGAACTCAAAAAGGGAGAAGCGTTTACGGACCAGAACGGGACGA
GCCATGAACTGAAGGACCCTTTATACGTTACGAAAAAGAAAGGCGAAGTTCTGAGTGAAAATGTAGACACGGACGGAATC
CTTAACATTAAAGGGGAGGATGGCAGGCTGCTTCAAGCCCTGCAAATCGCAGAACCATTCAGCGCGGAAATGACAAACGG
TACAGATGCTATTCAGGGTCAACCGCAAAGTAAAGGCGGATTGTTTTTATTTGTGTTCGGCCTAATCGGTTTATTGACGG
CCAGCTATGCGGTCAAGCGGATGAGGCAGCTGTAA

Upstream 100 bases:

>100_bases
AAGGCTTGTCAGCGACAGTTCATCATTGGTAAAATTTAAAAAGTGCTAAAAGACCTATTTTTATAAAAATTGCAAAAGAG
AAAGAAGGCAGGGTGACACG

Downstream 100 bases:

>100_bases
TATGATAAGGGAAGAGGCGAGCCGGCTCACAAGCCGGCCGTTTTTTTATTTGCCCTGAAAAGGCAAATTTATCTTATAAT
GGAAACAAGCCAATGAAAGG

Product: hypothetical protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNVKRSLLFIIVLCLTLFFHSSAQAGEKNKPEIKSVSAILIDGKSGQVLYEKNSRKKMYPASITKIATAIYAIENGNLED
RVKVSKNAAQTEGSSVYLEEGESVPLKRLLQGLLMNSGNDAGTAIAEHLSGSVKQFADDLNAFIAEHAGVHDTHFMNPHG
LFDKNHYTTSADMARITEYAMKNKTFRALFSEKQTDWHGQTWDTTLKNHHRMLTGEIPYKGITGGKTGFVDESKHTLVTT
ATRGSLDLIAVVMKADSKKMMYSDTKALLDEGFHNFETQELKKGEAFTDQNGTSHELKDPLYVTKKKGEVLSENVDTDGI
LNIKGEDGRLLQALQIAEPFSAEMTNGTDAIQGQPQSKGGLFLFVFGLIGLLTASYAVKRMRQL

Sequences:

>Translated_384_residues
MNVKRSLLFIIVLCLTLFFHSSAQAGEKNKPEIKSVSAILIDGKSGQVLYEKNSRKKMYPASITKIATAIYAIENGNLED
RVKVSKNAAQTEGSSVYLEEGESVPLKRLLQGLLMNSGNDAGTAIAEHLSGSVKQFADDLNAFIAEHAGVHDTHFMNPHG
LFDKNHYTTSADMARITEYAMKNKTFRALFSEKQTDWHGQTWDTTLKNHHRMLTGEIPYKGITGGKTGFVDESKHTLVTT
ATRGSLDLIAVVMKADSKKMMYSDTKALLDEGFHNFETQELKKGEAFTDQNGTSHELKDPLYVTKKKGEVLSENVDTDGI
LNIKGEDGRLLQALQIAEPFSAEMTNGTDAIQGQPQSKGGLFLFVFGLIGLLTASYAVKRMRQL
>Mature_384_residues
MNVKRSLLFIIVLCLTLFFHSSAQAGEKNKPEIKSVSAILIDGKSGQVLYEKNSRKKMYPASITKIATAIYAIENGNLED
RVKVSKNAAQTEGSSVYLEEGESVPLKRLLQGLLMNSGNDAGTAIAEHLSGSVKQFADDLNAFIAEHAGVHDTHFMNPHG
LFDKNHYTTSADMARITEYAMKNKTFRALFSEKQTDWHGQTWDTTLKNHHRMLTGEIPYKGITGGKTGFVDESKHTLVTT
ATRGSLDLIAVVMKADSKKMMYSDTKALLDEGFHNFETQELKKGEAFTDQNGTSHELKDPLYVTKKKGEVLSENVDTDGI
LNIKGEDGRLLQALQIAEPFSAEMTNGTDAIQGQPQSKGGLFLFVFGLIGLLTASYAVKRMRQL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=346, Percent_Identity=30.9248554913295, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87082022, Length=309, Percent_Identity=32.6860841423948, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787062, Length=304, Percent_Identity=30.9210526315789, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87082054, Length=279, Percent_Identity=27.9569892473118, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42345; Mature: 42345

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVKRSLLFIIVLCLTLFFHSSAQAGEKNKPEIKSVSAILIDGKSGQVLYEKNSRKKMYP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCEEEEEEECCCCCEEEECCCCCCCCC
ASITKIATAIYAIENGNLEDRVKVSKNAAQTEGSSVYLEEGESVPLKRLLQGLLMNSGND
HHHHHHHHHHHEEECCCCHHHHHECCCHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCC
AGTAIAEHLSGSVKQFADDLNAFIAEHAGVHDTHFMNPHGLFDKNHYTTSADMARITEYA
HHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
MKNKTFRALFSEKQTDWHGQTWDTTLKNHHRMLTGEIPYKGITGGKTGFVDESKHTLVTT
HHCHHHHHHHHCCCCCCCCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
ATRGSLDLIAVVMKADSKKMMYSDTKALLDEGFHNFETQELKKGEAFTDQNGTSHELKDP
ECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
LYVTKKKGEVLSENVDTDGILNIKGEDGRLLQALQIAEPFSAEMTNGTDAIQGQPQSKGG
EEEECCCCCHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCC
LFLFVFGLIGLLTASYAVKRMRQL
EEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNVKRSLLFIIVLCLTLFFHSSAQAGEKNKPEIKSVSAILIDGKSGQVLYEKNSRKKMYP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCEEEEEEECCCCCEEEECCCCCCCCC
ASITKIATAIYAIENGNLEDRVKVSKNAAQTEGSSVYLEEGESVPLKRLLQGLLMNSGND
HHHHHHHHHHHEEECCCCHHHHHECCCHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCC
AGTAIAEHLSGSVKQFADDLNAFIAEHAGVHDTHFMNPHGLFDKNHYTTSADMARITEYA
HHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
MKNKTFRALFSEKQTDWHGQTWDTTLKNHHRMLTGEIPYKGITGGKTGFVDESKHTLVTT
HHCHHHHHHHHCCCCCCCCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
ATRGSLDLIAVVMKADSKKMMYSDTKALLDEGFHNFETQELKKGEAFTDQNGTSHELKDP
ECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
LYVTKKKGEVLSENVDTDGILNIKGEDGRLLQALQIAEPFSAEMTNGTDAIQGQPQSKGG
EEEECCCCCHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCC
LFLFVFGLIGLLTASYAVKRMRQL
EEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]