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The map label for this gene is yufM [H]

Identifier: 52787031

GI number: 52787031

Start: 3192266

End: 3192973

Strand: Direct

Name: yufM [H]

Synonym: BLi03335

Alternate gene names: 52787031

Gene position: 3192266-3192973 (Clockwise)

Preceding gene: 52787030

Following gene: 52787032

Centisome position: 75.6

GC content: 45.2

Gene sequence:

>708_bases
ATGATTGATGTATTAATTGTTGAAGACGACCCGATGGTAAGAGAGCTGAATAAACGTTACCTCAGCCAAGTAGAGGGATT
TCAGCTGAAGGGCATTGCCGCTTCTTTTCAAGAGGCTCTCACCTTCCTCCGGGAAGAGCATATCGATCTGATCCTGCTCG
ACATTTATATGCCTGGCAGGAACGGCGTCGAACTGCTCACTGAGATCAGACGCCAAAATCAAGCTGTCGACGTGATCGTC
ATCTCAGCGGCCAGTGAAATGGATGTTGTTCAAAAAACGCTGCGGTTCGGGGCCGTCGATTACTTAATCAAGCCGTTCGA
ATTTGAGCGGTTTCAAAGCGCGCTCACTGATTACAAACGGAAGCAGCAGCTCTATGCTAGCAACCGGAATATCAGCCAAA
AAGAGCTGGATTTTGAACTTTTTCAAAAAAAACGCGCGCCCGAAAAGGTACATTTGCCAAAAGGGCTGACCAAAAGCACG
CTGAAGCTCATTTGGTCAAGCATCAGATCGTTTGACAGCTTGACGTTCACAACGGAAGATTTGGCCAATCACACGGAAAT
CTCTCAAGTTTCAATCCGCAAATACTTAAAGTTTCTGGAGGAAATCGAAGTTCTCAACGTAGAAATGGCCTACGGGACGA
TCGGCAGGCCGGTTTTCCAATATAAATTAAATACAAATGACTTGAATGTGATTAACCAATATCTATAA

Upstream 100 bases:

>100_bases
AGCCTTGAAAATTTAAATGGACATATCGTCGTGACGAGCGAAAAGAATGAAGGAACGACATTCAGTCTCCGCATTCCGTA
TGAACCAAAGGAGGATGACC

Downstream 100 bases:

>100_bases
CATGTCCCCGCCCGCTGCTCTCTAAAGACAGCGGGCGTTTTTTTTGTAACGGCCACATTTGACTCGCCATCATTGCCGGA
CAAGGATTTATCGCGCTCGC

Product: YufM

Products: NA

Alternate protein names: Malate response regulator [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MIDVLIVEDDPMVRELNKRYLSQVEGFQLKGIAASFQEALTFLREEHIDLILLDIYMPGRNGVELLTEIRRQNQAVDVIV
ISAASEMDVVQKTLRFGAVDYLIKPFEFERFQSALTDYKRKQQLYASNRNISQKELDFELFQKKRAPEKVHLPKGLTKST
LKLIWSSIRSFDSLTFTTEDLANHTEISQVSIRKYLKFLEEIEVLNVEMAYGTIGRPVFQYKLNTNDLNVINQYL

Sequences:

>Translated_235_residues
MIDVLIVEDDPMVRELNKRYLSQVEGFQLKGIAASFQEALTFLREEHIDLILLDIYMPGRNGVELLTEIRRQNQAVDVIV
ISAASEMDVVQKTLRFGAVDYLIKPFEFERFQSALTDYKRKQQLYASNRNISQKELDFELFQKKRAPEKVHLPKGLTKST
LKLIWSSIRSFDSLTFTTEDLANHTEISQVSIRKYLKFLEEIEVLNVEMAYGTIGRPVFQYKLNTNDLNVINQYL
>Mature_235_residues
MIDVLIVEDDPMVRELNKRYLSQVEGFQLKGIAASFQEALTFLREEHIDLILLDIYMPGRNGVELLTEIRRQNQAVDVIV
ISAASEMDVVQKTLRFGAVDYLIKPFEFERFQSALTDYKRKQQLYASNRNISQKELDFELFQKKRAPEKVHLPKGLTKST
LKLIWSSIRSFDSLTFTTEDLANHTEISQVSIRKYLKFLEEIEVLNVEMAYGTIGRPVFQYKLNTNDLNVINQYL

Specific function: Member of a two-component regulatory system malK/malR. Activates transcription of maeA, maeN and yflS in presence of malate by binding to their promoter region [H]

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790566, Length=239, Percent_Identity=41.0041841004184, Blast_Score=188, Evalue=2e-49,
Organism=Escherichia coli, GI1786838, Length=217, Percent_Identity=31.3364055299539, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788222, Length=153, Percent_Identity=30.718954248366, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1790437, Length=118, Percent_Identity=33.8983050847458, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788550, Length=110, Percent_Identity=36.3636363636364, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=32.2834645669291, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1787487, Length=104, Percent_Identity=37.5, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789809, Length=103, Percent_Identity=33.0097087378641, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 27347; Mature: 27347

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVLIVEDDPMVRELNKRYLSQVEGFQLKGIAASFQEALTFLREEHIDLILLDIYMPGR
CEEEEEECCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
NGVELLTEIRRQNQAVDVIVISAASEMDVVQKTLRFGAVDYLIKPFEFERFQSALTDYKR
CCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KQQLYASNRNISQKELDFELFQKKRAPEKVHLPKGLTKSTLKLIWSSIRSFDSLTFTTED
HHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCCEEEHHH
LANHTEISQVSIRKYLKFLEEIEVLNVEMAYGTIGRPVFQYKLNTNDLNVINQYL
HHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEECCCHHHHHHHCC
>Mature Secondary Structure
MIDVLIVEDDPMVRELNKRYLSQVEGFQLKGIAASFQEALTFLREEHIDLILLDIYMPGR
CEEEEEECCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
NGVELLTEIRRQNQAVDVIVISAASEMDVVQKTLRFGAVDYLIKPFEFERFQSALTDYKR
CCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KQQLYASNRNISQKELDFELFQKKRAPEKVHLPKGLTKSTLKLIWSSIRSFDSLTFTTED
HHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCCEEEHHH
LANHTEISQVSIRKYLKFLEEIEVLNVEMAYGTIGRPVFQYKLNTNDLNVINQYL
HHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]