Click here to switch to the map view.

The map label for this gene is yufL [H]

Identifier: 52787030

GI number: 52787030

Start: 3190672

End: 3192273

Strand: Direct

Name: yufL [H]

Synonym: BLi03334

Alternate gene names: 52787030

Gene position: 3190672-3192273 (Clockwise)

Preceding gene: 52787028

Following gene: 52787031

Centisome position: 75.56

GC content: 48.56

Gene sequence:

>1602_bases
TTGAAAAAAACATTAAAACTGCAGACACGGTTAACGATATTTGTCTGCGTCGTCGTTTTAATTTCTTTATTGATCGCGTT
TTTTATGGTCGGCTCTGAGACCGCAAGGAACATTCGGGAGCAGGAGCAGCAGATTGCCCTGCAGACGGCGGAGATGGTCG
CCGAGGCTCCGATTACGGCACAATCGCTGGAAAGCGGCGAATACGAAGAGCTCCGGAAATACACCGCCCGCGTTCAAAAA
ATCACCCAGACTGAATTCGTCGTTGTCATGGATATGAACAGCATCCGCAAAACCCACCCCGATCCGAATAAAATCGGAAA
GAAATTTGCCGGCGGGGACGAAAAAGAAGTGCTGAAGGGCCGACGCCACATTAGTACGGCAAAAGGCACGCTCGGTGAAT
CACTGAGGGCTTTCGTTCCCATCTATAATAAAACGGGCACCCAGGTCGGAGCGGTAGCGGTCGGGATCACCCTTAACGAG
ATTGAGCAGATCACCCAGCAAAATCTCCGCCCTCTATATACGGTCATCGTCCTCAGCATCGTCGCCGGAATCATCGGAGC
TGTCATCGTGGCGAGGAAAGTGAAAACGATTATGTTCGGCATGGAACCATATGAAATTGCAACGCTTTTGAACGAACGAA
GCGCAATGCTTGAGTCGACAAAAGAAGGGATTCTCGCCGTTGATCGAACAGGAAAAATCAAGCTCGCCAATGCGGAAGCG
AAGCGCCTGTTTCAAAAAATGGGGATCGAAGAGAATCCGATCGACCGGGATGTACTCGACATCTTGCCAAACAGCCGACT
GAAACAAGTGATCGAAACACAGAAGCCCGTTCAGGACCGCGATGTCAGAATCAATGGCCTTGAACTTGTCTTTAATGAAG
TTCCGATTCACTTAAAAAAGGGAAACATCGTCGGAGCGATTGCGACATTCCGCGACAAAACCGAAGTGAAGCACTTAGCT
GAGCAGCTGTCCGGTGTTAAAATGTACGCAAACGCGCTCAGGGCGCAGTCGCACGAGTTTATGAACAAGCTCCACGTCAT
TCTCGGTCTTGTTCAATTAAAGAACTACGACGAGCTTGGAAACTATATAAAAGACATTGCGATCTATCAGCAGTCTGAAG
CGAGCGGAATTATCAACCATATCAAAAACTCGGTACTCGCCGGCTTTTTGCTCGGCAAACAGAGCTATATTCGGGAACAG
GGGGCCTCGTTGGAGGTTCATTGCAGCACGAGGATTCCGGATACAGCCAACACAGCGGTGATTCACGATTTGATCACCGT
TATCGGGAACCTTCTCAACAATGCCCTTGATGCCGTTTCCGCCACTGAGAAGAAAAATATTTCGATTTCATTCCGCCATC
ATGACGGACAGCTTGATATTGAAGTCACGGATACGGGAGTCGGCATATCCAAGGAGGAACAGCAGAAAATGTTTGACCAA
GGCTTTTCAACAAAGGGCCCCAATCGGGGGTTCGGTCTTTATTTCGTCGAACAAAGCCTTGAAAATTTAAATGGACATAT
CGTCGTGACGAGCGAAAAGAATGAAGGAACGACATTCAGTCTCCGCATTCCGTATGAACCAAAGGAGGATGACCATGATT
GA

Upstream 100 bases:

>100_bases
TCTGCTAATGCGGCAGAAAGGTGCAGGATTCTTCACAAATTCGCCATTTACTTGTAAAGTATTATGTGTTGACTAATCAA
TAGATCGCGAGGAATCGCCG

Downstream 100 bases:

>100_bases
TGTATTAATTGTTGAAGACGACCCGATGGTAAGAGAGCTGAATAAACGTTACCTCAGCCAAGTAGAGGGATTTCAGCTGA
AGGGCATTGCCGCTTCTTTT

Product: sensory histidine kinase DcuS

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKKTLKLQTRLTIFVCVVVLISLLIAFFMVGSETARNIREQEQQIALQTAEMVAEAPITAQSLESGEYEELRKYTARVQK
ITQTEFVVVMDMNSIRKTHPDPNKIGKKFAGGDEKEVLKGRRHISTAKGTLGESLRAFVPIYNKTGTQVGAVAVGITLNE
IEQITQQNLRPLYTVIVLSIVAGIIGAVIVARKVKTIMFGMEPYEIATLLNERSAMLESTKEGILAVDRTGKIKLANAEA
KRLFQKMGIEENPIDRDVLDILPNSRLKQVIETQKPVQDRDVRINGLELVFNEVPIHLKKGNIVGAIATFRDKTEVKHLA
EQLSGVKMYANALRAQSHEFMNKLHVILGLVQLKNYDELGNYIKDIAIYQQSEASGIINHIKNSVLAGFLLGKQSYIREQ
GASLEVHCSTRIPDTANTAVIHDLITVIGNLLNNALDAVSATEKKNISISFRHHDGQLDIEVTDTGVGISKEEQQKMFDQ
GFSTKGPNRGFGLYFVEQSLENLNGHIVVTSEKNEGTTFSLRIPYEPKEDDHD

Sequences:

>Translated_533_residues
MKKTLKLQTRLTIFVCVVVLISLLIAFFMVGSETARNIREQEQQIALQTAEMVAEAPITAQSLESGEYEELRKYTARVQK
ITQTEFVVVMDMNSIRKTHPDPNKIGKKFAGGDEKEVLKGRRHISTAKGTLGESLRAFVPIYNKTGTQVGAVAVGITLNE
IEQITQQNLRPLYTVIVLSIVAGIIGAVIVARKVKTIMFGMEPYEIATLLNERSAMLESTKEGILAVDRTGKIKLANAEA
KRLFQKMGIEENPIDRDVLDILPNSRLKQVIETQKPVQDRDVRINGLELVFNEVPIHLKKGNIVGAIATFRDKTEVKHLA
EQLSGVKMYANALRAQSHEFMNKLHVILGLVQLKNYDELGNYIKDIAIYQQSEASGIINHIKNSVLAGFLLGKQSYIREQ
GASLEVHCSTRIPDTANTAVIHDLITVIGNLLNNALDAVSATEKKNISISFRHHDGQLDIEVTDTGVGISKEEQQKMFDQ
GFSTKGPNRGFGLYFVEQSLENLNGHIVVTSEKNEGTTFSLRIPYEPKEDDHD
>Mature_533_residues
MKKTLKLQTRLTIFVCVVVLISLLIAFFMVGSETARNIREQEQQIALQTAEMVAEAPITAQSLESGEYEELRKYTARVQK
ITQTEFVVVMDMNSIRKTHPDPNKIGKKFAGGDEKEVLKGRRHISTAKGTLGESLRAFVPIYNKTGTQVGAVAVGITLNE
IEQITQQNLRPLYTVIVLSIVAGIIGAVIVARKVKTIMFGMEPYEIATLLNERSAMLESTKEGILAVDRTGKIKLANAEA
KRLFQKMGIEENPIDRDVLDILPNSRLKQVIETQKPVQDRDVRINGLELVFNEVPIHLKKGNIVGAIATFRDKTEVKHLA
EQLSGVKMYANALRAQSHEFMNKLHVILGLVQLKNYDELGNYIKDIAIYQQSEASGIINHIKNSVLAGFLLGKQSYIREQ
GASLEVHCSTRIPDTANTAVIHDLITVIGNLLNNALDAVSATEKKNISISFRHHDGQLDIEVTDTGVGISKEEQQKMFDQ
GFSTKGPNRGFGLYFVEQSLENLNGHIVVTSEKNEGTTFSLRIPYEPKEDDHD

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=531, Percent_Identity=36.7231638418079, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1786837, Length=529, Percent_Identity=27.2211720226843, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788549, Length=353, Percent_Identity=24.6458923512748, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59302; Mature: 59302

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLKLQTRLTIFVCVVVLISLLIAFFMVGSETARNIREQEQQIALQTAEMVAEAPITA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
QSLESGEYEELRKYTARVQKITQTEFVVVMDMNSIRKTHPDPNKIGKKFAGGDEKEVLKG
HHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCHHHHHHHCCCCCHHHHHHH
RRHISTAKGTLGESLRAFVPIYNKTGTQVGAVAVGITLNEIEQITQQNLRPLYTVIVLSI
HHHHHHHHHHHHHHHHHHHCEECCCCCEEEHEEEEEEHHHHHHHHHHCCHHHHHHHHHHH
VAGIIGAVIVARKVKTIMFGMEPYEIATLLNERSAMLESTKEGILAVDRTGKIKLANAEA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECHHH
KRLFQKMGIEENPIDRDVLDILPNSRLKQVIETQKPVQDRDVRINGLELVFNEVPIHLKK
HHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEHHEEEEECCCEEEEC
GNIVGAIATFRDKTEVKHLAEQLSGVKMYANALRAQSHEFMNKLHVILGLVQLKNYDELG
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NYIKDIAIYQQSEASGIINHIKNSVLAGFLLGKQSYIREQGASLEVHCSTRIPDTANTAV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
IHDLITVIGNLLNNALDAVSATEKKNISISFRHHDGQLDIEVTDTGVGISKEEQQKMFDQ
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHC
GFSTKGPNRGFGLYFVEQSLENLNGHIVVTSEKNEGTTFSLRIPYEPKEDDHD
CCCCCCCCCCCCCEEHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKTLKLQTRLTIFVCVVVLISLLIAFFMVGSETARNIREQEQQIALQTAEMVAEAPITA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
QSLESGEYEELRKYTARVQKITQTEFVVVMDMNSIRKTHPDPNKIGKKFAGGDEKEVLKG
HHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCHHHHHHHCCCCCHHHHHHH
RRHISTAKGTLGESLRAFVPIYNKTGTQVGAVAVGITLNEIEQITQQNLRPLYTVIVLSI
HHHHHHHHHHHHHHHHHHHCEECCCCCEEEHEEEEEEHHHHHHHHHHCCHHHHHHHHHHH
VAGIIGAVIVARKVKTIMFGMEPYEIATLLNERSAMLESTKEGILAVDRTGKIKLANAEA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECHHH
KRLFQKMGIEENPIDRDVLDILPNSRLKQVIETQKPVQDRDVRINGLELVFNEVPIHLKK
HHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEHHEEEEECCCEEEEC
GNIVGAIATFRDKTEVKHLAEQLSGVKMYANALRAQSHEFMNKLHVILGLVQLKNYDELG
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NYIKDIAIYQQSEASGIINHIKNSVLAGFLLGKQSYIREQGASLEVHCSTRIPDTANTAV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
IHDLITVIGNLLNNALDAVSATEKKNISISFRHHDGQLDIEVTDTGVGISKEEQQKMFDQ
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHC
GFSTKGPNRGFGLYFVEQSLENLNGHIVVTSEKNEGTTFSLRIPYEPKEDDHD
CCCCCCCCCCCCCEEHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]