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The map label for this gene is menF [H]

Identifier: 52786947

GI number: 52786947

Start: 3104044

End: 3105468

Strand: Reverse

Name: menF [H]

Synonym: BLi03223

Alternate gene names: 52786947

Gene position: 3105468-3104044 (Counterclockwise)

Preceding gene: 52786950

Following gene: 52786946

Centisome position: 73.54

GC content: 48.14

Gene sequence:

>1425_bases
ATGGTGACAACAGTGCAGCGCACTTTCCTCAAGGAAGCGCCTGAAGCATTAAACATCGTCAAAAAAGTCAACCATGCTGT
TTTAATAAGCTATTCCAGAAAAATTGAGAGCATAGATGCTCTTTCCTTTTTCATAAACGGGAAAGAACAATTTGCCGGGG
AACGGTTCTTCTGGTCTGATCCGGACGGCGGGCTGACGCTTGTCGGGCTGGGAAAGGAAATATCGGTTTCCACAGATCTG
AAGGATGCCGGACGTTATCAGGCTGTACATGAAAAATGGGAGCGCTTAAAAGAGAAAACCTATCACTTTCATGAAGATCA
AAGCAGAGGTCAGGCAGCGGTGGGGCCATTGCTGTTTGGCGGTTTTTCATTTGATCCGCTGGAAGATCGCAAGCAGCATT
GGAACGGCTACCAGGAAGGCGCTTTTTTTATTCCCGCCGCTATGCTGACACAGGACGGAGAGGAAACATTCATAACGGTC
AATAGATGGAAGACTCCTGAAGAAGACGTGCGCAAAGTACTTGAAGAAATAAAGGGCGCTGCTTCACAGCTGACCAAGGC
GGACGATTCCCGTTATCCGGAAAAAGGTCATACGCAATATACCGCCAAGGAAGAGCTTGATGTTCCTGAATGGCTTGAAG
CGGTCCGGCGGGCGACAGACGACATTAAAAACGGGAAGTACGATAAAGTCGTGCTCGCCAGAGAGATCCTTTTAACGTTC
GATCAGCCTGTCGAACTTCACAGAGTATTATTCAATCTGATGTCTGAACAAAAAACGAGCTATATATTCGCCATTGAAGA
AAACGGCAAAAGTTTTGTGGGCGCGTCTCCGGAACGTCTTGTCAAAAAAGACGGAGAGAACGTTCTGTCGACCTGCCTCG
CCGGTTCAATCAAGAGAGGCGAAAGTGAGCAGGAAGACCGGATGCTCGGTCTTGAGCTGTTAAACGATGAAAAAAATCTA
ATCGAACATGACATTGTCGTCAATATGATTCATCAGGCGCTTTTGTCAAATTGCAGCAATATCAAAAAGCCGGCCAAGCC
GGGACTTTACAAAACAAAAAATGTGCAGCATTTGTATACGCCGATTGTCGGCAAAATGAATGCGGAGTCCTCGATTTTTG
ATTTAATCGAGAAGCTTCACCCAACTCCTGCGCTCGGAGGAGCACCACAGCAAAAAGCGATTGAGGTTATCAGGGAGTTG
GAACCGATGTCAAGGGGCTGGTATGCGGCACCGGCGGGCTGGATCGACATGTCCGGCAATGGGGAATTCGCGGTGGCGAT
CAGGTCGGGACTTATCGATTCAAATGCGGCCAGAATTTTCGCCGGCTGCGGGGTTGTGGCTGATTCCGATCCTCTTATGG
AATACGAAGAAACACAAATTAAGATGAAGCCGATGCTGTCTGCTCTTGGAGGCGATATCCGTTGA

Upstream 100 bases:

>100_bases
TAGGTGGTGGCAGTTGTTAATAAAGTGTCGTTTTGTTGACACATACCGTTCACAGGTGTATGGTTATAAAAGCATTTTAC
GGATTTTGGAGGAATGTATC

Downstream 100 bases:

>100_bases
CTGAAAATCCGATTACAAAATATATAGGGAATTTTATTGATGAGCTTTCCCTTGCAGGCGTGACAGAAGCGGTCATCTGC
CCGGGATCAAGATCGACGCC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MVTTVQRTFLKEAPEALNIVKKVNHAVLISYSRKIESIDALSFFINGKEQFAGERFFWSDPDGGLTLVGLGKEISVSTDL
KDAGRYQAVHEKWERLKEKTYHFHEDQSRGQAAVGPLLFGGFSFDPLEDRKQHWNGYQEGAFFIPAAMLTQDGEETFITV
NRWKTPEEDVRKVLEEIKGAASQLTKADDSRYPEKGHTQYTAKEELDVPEWLEAVRRATDDIKNGKYDKVVLAREILLTF
DQPVELHRVLFNLMSEQKTSYIFAIEENGKSFVGASPERLVKKDGENVLSTCLAGSIKRGESEQEDRMLGLELLNDEKNL
IEHDIVVNMIHQALLSNCSNIKKPAKPGLYKTKNVQHLYTPIVGKMNAESSIFDLIEKLHPTPALGGAPQQKAIEVIREL
EPMSRGWYAAPAGWIDMSGNGEFAVAIRSGLIDSNAARIFAGCGVVADSDPLMEYEETQIKMKPMLSALGGDIR

Sequences:

>Translated_474_residues
MVTTVQRTFLKEAPEALNIVKKVNHAVLISYSRKIESIDALSFFINGKEQFAGERFFWSDPDGGLTLVGLGKEISVSTDL
KDAGRYQAVHEKWERLKEKTYHFHEDQSRGQAAVGPLLFGGFSFDPLEDRKQHWNGYQEGAFFIPAAMLTQDGEETFITV
NRWKTPEEDVRKVLEEIKGAASQLTKADDSRYPEKGHTQYTAKEELDVPEWLEAVRRATDDIKNGKYDKVVLAREILLTF
DQPVELHRVLFNLMSEQKTSYIFAIEENGKSFVGASPERLVKKDGENVLSTCLAGSIKRGESEQEDRMLGLELLNDEKNL
IEHDIVVNMIHQALLSNCSNIKKPAKPGLYKTKNVQHLYTPIVGKMNAESSIFDLIEKLHPTPALGGAPQQKAIEVIREL
EPMSRGWYAAPAGWIDMSGNGEFAVAIRSGLIDSNAARIFAGCGVVADSDPLMEYEETQIKMKPMLSALGGDIR
>Mature_474_residues
MVTTVQRTFLKEAPEALNIVKKVNHAVLISYSRKIESIDALSFFINGKEQFAGERFFWSDPDGGLTLVGLGKEISVSTDL
KDAGRYQAVHEKWERLKEKTYHFHEDQSRGQAAVGPLLFGGFSFDPLEDRKQHWNGYQEGAFFIPAAMLTQDGEETFITV
NRWKTPEEDVRKVLEEIKGAASQLTKADDSRYPEKGHTQYTAKEELDVPEWLEAVRRATDDIKNGKYDKVVLAREILLTF
DQPVELHRVLFNLMSEQKTSYIFAIEENGKSFVGASPERLVKKDGENVLSTCLAGSIKRGESEQEDRMLGLELLNDEKNL
IEHDIVVNMIHQALLSNCSNIKKPAKPGLYKTKNVQHLYTPIVGKMNAESSIFDLIEKLHPTPALGGAPQQKAIEVIREL
EPMSRGWYAAPAGWIDMSGNGEFAVAIRSGLIDSNAARIFAGCGVVADSDPLMEYEETQIKMKPMLSALGGDIR

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=425, Percent_Identity=31.5294117647059, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI87082077, Length=248, Percent_Identity=32.6612903225806, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787518, Length=374, Percent_Identity=25.9358288770053, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788114, Length=259, Percent_Identity=24.7104247104247, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=307, Percent_Identity=29.9674267100977, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 53094; Mature: 53094

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTTVQRTFLKEAPEALNIVKKVNHAVLISYSRKIESIDALSFFINGKEQFAGERFFWSD
CCCHHHHHHHHHCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHEECCHHHHCCCCCEECC
PDGGLTLVGLGKEISVSTDLKDAGRYQAVHEKWERLKEKTYHFHEDQSRGQAAVGPLLFG
CCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
GFSFDPLEDRKQHWNGYQEGAFFIPAAMLTQDGEETFITVNRWKTPEEDVRKVLEEIKGA
CCCCCCHHHHHHHCCCCCCCCEEECHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
ASQLTKADDSRYPEKGHTQYTAKEELDVPEWLEAVRRATDDIKNGKYDKVVLAREILLTF
HHHHHHCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
DQPVELHRVLFNLMSEQKTSYIFAIEENGKSFVGASPERLVKKDGENVLSTCLAGSIKRG
CCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCC
ESEQEDRMLGLELLNDEKNLIEHDIVVNMIHQALLSNCSNIKKPAKPGLYKTKNVQHLYT
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
PIVGKMNAESSIFDLIEKLHPTPALGGAPQQKAIEVIRELEPMSRGWYAAPAGWIDMSGN
HHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHCCCEECCCCEEEECCC
GEFAVAIRSGLIDSNAARIFAGCGVVADSDPLMEYEETQIKMKPMLSALGGDIR
CCEEEEEECCCCCCCCHHEEECCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVTTVQRTFLKEAPEALNIVKKVNHAVLISYSRKIESIDALSFFINGKEQFAGERFFWSD
CCCHHHHHHHHHCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHEECCHHHHCCCCCEECC
PDGGLTLVGLGKEISVSTDLKDAGRYQAVHEKWERLKEKTYHFHEDQSRGQAAVGPLLFG
CCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
GFSFDPLEDRKQHWNGYQEGAFFIPAAMLTQDGEETFITVNRWKTPEEDVRKVLEEIKGA
CCCCCCHHHHHHHCCCCCCCCEEECHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
ASQLTKADDSRYPEKGHTQYTAKEELDVPEWLEAVRRATDDIKNGKYDKVVLAREILLTF
HHHHHHCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
DQPVELHRVLFNLMSEQKTSYIFAIEENGKSFVGASPERLVKKDGENVLSTCLAGSIKRG
CCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCC
ESEQEDRMLGLELLNDEKNLIEHDIVVNMIHQALLSNCSNIKKPAKPGLYKTKNVQHLYT
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
PIVGKMNAESSIFDLIEKLHPTPALGGAPQQKAIEVIRELEPMSRGWYAAPAGWIDMSGN
HHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHCCCEECCCCEEEECCC
GEFAVAIRSGLIDSNAARIFAGCGVVADSDPLMEYEETQIKMKPMLSALGGDIR
CCEEEEEECCCCCCCCHHEEECCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]