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The map label for this gene is menD

Identifier: 52786946

GI number: 52786946

Start: 3102305

End: 3104047

Strand: Reverse

Name: menD

Synonym: BLi03222

Alternate gene names: 52786946

Gene position: 3104047-3102305 (Counterclockwise)

Preceding gene: 52786947

Following gene: 52786945

Centisome position: 73.51

GC content: 50.26

Gene sequence:

>1743_bases
TTGACTGAAAATCCGATTACAAAATATATAGGGAATTTTATTGATGAGCTTTCCCTTGCAGGCGTGACAGAAGCGGTCAT
CTGCCCGGGATCAAGATCGACGCCCCTTGCGATTTTGGCCGCGAGTCATCCGGACATCAATGTTCATATCCATGTGGATG
AAAGATCCGCTGCTTTTTTTGCAATGGGCATGGCAAAGGCAATGCGGAAGCCAGTCGCATTGATCTGTACATCCGGGACG
GCTGCAGCGAATTTCTACCCGGCTGTGATCGAGGCTCATTATTCTAGAATACCGCTTATGATCTTAACGGCTGATCGGCC
GCATGAACTGAGAGAAGTAGGAGCACCTCAGGCGATTGATCAGCAATTCTTATTCGGAAACTTTGTTAAGTTTTTCACAG
ACTCGGCTTTGCCTGACAGTCATCCTGACATGCTGGCGTACATCCGGTCTTTGGCAGCACGTGCAGCCGGAGAAGCCAAA
AAAGCGCCGCGCGGGCCCGTGCATATCAACTTTCCGCTGAGAGAGCCGCTCATGCCGAACCTTGAGGATGAACCTTTTCA
AAGGCTGAGAAGCGGCAGTCATGTGACGGTTGAAAGCGGCCGCTTCCGTGCTGATCGGCATGAGCTTGTAGCGCTGAGTG
CTCTGATTAAAGAGACCGAAAAAGGCGTAATCGTCTGCGGAGAGCTGCAAGATGATGATGCAAAAGAACATATTGTAGCT
CTTTCACAGGCAGCAGAATACCCAATTTTGGCTGACCCGCTCTCAAACTTGAGAAACGGCACCCATTCAAAAGAAACCAT
TATCGATGCATACGACTCATTATTGAAAGAAGAACAGCTGAAACGCGAGCTCATGCCTGAGCTTGTCATCCGCTTCGGTG
CGATGCCGGTTTCCAAGCCGCTTTTTCAGCTGCTGAAGCAAAATCCGAGCATTAGGCAAATCGTCGTTGATCCAGATGGA
GGATGGCGCGATCCGACTCAGCTCAGCGCACATATGCTGCATTGCACGGAAGAGGAGATTGCTGTTCTTGCGGATTCCGC
TGTCAATTCAGAAAATAGGACAGGCAACTGGCTTGAGAAGTGGCGGTTTGCCAACAGCCGCTTCCGCGCTCTTTTAAGAA
ATCTTGAAGCTGAGGATCTTACCTTTGAAGGAAATATCTACCGGAACCTTCAGCATTTGATTCCGGACAACAGCTCCGTG
TTTGTCGGAAACAGCATGCCGATCCGCGATGCGGACACATTTTTTGAAAATCAGGAACGGACCTTCCGGATTTTTGCCAA
TAGAGGGGCGAACGGGATAGACGGTGTTGTCTCAACGGCGCTGGGCATATATGCCGCAACGAAAGAACCGGTAACCCTTG
TCATCGGCGATCTGTCATTTTATCACGACTTAAACGGCCTCCTCGTCAGCAAAACGCTCGATATTCCGCTGACTGTCGTT
CTCGTCAACAATGATGGCGGCGGCATTTTCTCATTTCTTCCCCAATCTGCTGAAGAAAGGTACTTCGAGCTCTTGTTCGG
AACGCCGACGGGTCTCGAATTCGAGCATGCTGCCAAGCTGTATGGGGGACGCTATATATGCCCTGAATCATCCGCACATT
TTGCCGATGCGTATTTATCCTCCGTCAACCGCCGCGGCTTGGATATCATTGAGGTGAAAACGAAGCGCGATGATCGTGTG
GAGCAGCATCGCCGGATGCTTGATTCCGCGCTGCATGAAATAAGAAAAGAGTGGCCGTTTTGA

Upstream 100 bases:

>100_bases
CCGGCTGCGGGGTTGTGGCTGATTCCGATCCTCTTATGGAATACGAAGAAACACAAATTAAGATGAAGCCGATGCTGTCT
GCTCTTGGAGGCGATATCCG

Downstream 100 bases:

>100_bases
AATTGACACTGAAAGACGGCGTTTCCTATAAAATTGAAGACAATGGCCTGTCTGCAGAAAAAACGGCGGTGTTTTTGCAT
GGCTTTACAGGAAGCGCGGC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MTENPITKYIGNFIDELSLAGVTEAVICPGSRSTPLAILAASHPDINVHIHVDERSAAFFAMGMAKAMRKPVALICTSGT
AAANFYPAVIEAHYSRIPLMILTADRPHELREVGAPQAIDQQFLFGNFVKFFTDSALPDSHPDMLAYIRSLAARAAGEAK
KAPRGPVHINFPLREPLMPNLEDEPFQRLRSGSHVTVESGRFRADRHELVALSALIKETEKGVIVCGELQDDDAKEHIVA
LSQAAEYPILADPLSNLRNGTHSKETIIDAYDSLLKEEQLKRELMPELVIRFGAMPVSKPLFQLLKQNPSIRQIVVDPDG
GWRDPTQLSAHMLHCTEEEIAVLADSAVNSENRTGNWLEKWRFANSRFRALLRNLEAEDLTFEGNIYRNLQHLIPDNSSV
FVGNSMPIRDADTFFENQERTFRIFANRGANGIDGVVSTALGIYAATKEPVTLVIGDLSFYHDLNGLLVSKTLDIPLTVV
LVNNDGGGIFSFLPQSAEERYFELLFGTPTGLEFEHAAKLYGGRYICPESSAHFADAYLSSVNRRGLDIIEVKTKRDDRV
EQHRRMLDSALHEIRKEWPF

Sequences:

>Translated_580_residues
MTENPITKYIGNFIDELSLAGVTEAVICPGSRSTPLAILAASHPDINVHIHVDERSAAFFAMGMAKAMRKPVALICTSGT
AAANFYPAVIEAHYSRIPLMILTADRPHELREVGAPQAIDQQFLFGNFVKFFTDSALPDSHPDMLAYIRSLAARAAGEAK
KAPRGPVHINFPLREPLMPNLEDEPFQRLRSGSHVTVESGRFRADRHELVALSALIKETEKGVIVCGELQDDDAKEHIVA
LSQAAEYPILADPLSNLRNGTHSKETIIDAYDSLLKEEQLKRELMPELVIRFGAMPVSKPLFQLLKQNPSIRQIVVDPDG
GWRDPTQLSAHMLHCTEEEIAVLADSAVNSENRTGNWLEKWRFANSRFRALLRNLEAEDLTFEGNIYRNLQHLIPDNSSV
FVGNSMPIRDADTFFENQERTFRIFANRGANGIDGVVSTALGIYAATKEPVTLVIGDLSFYHDLNGLLVSKTLDIPLTVV
LVNNDGGGIFSFLPQSAEERYFELLFGTPTGLEFEHAAKLYGGRYICPESSAHFADAYLSSVNRRGLDIIEVKTKRDDRV
EQHRRMLDSALHEIRKEWPF
>Mature_579_residues
TENPITKYIGNFIDELSLAGVTEAVICPGSRSTPLAILAASHPDINVHIHVDERSAAFFAMGMAKAMRKPVALICTSGTA
AANFYPAVIEAHYSRIPLMILTADRPHELREVGAPQAIDQQFLFGNFVKFFTDSALPDSHPDMLAYIRSLAARAAGEAKK
APRGPVHINFPLREPLMPNLEDEPFQRLRSGSHVTVESGRFRADRHELVALSALIKETEKGVIVCGELQDDDAKEHIVAL
SQAAEYPILADPLSNLRNGTHSKETIIDAYDSLLKEEQLKRELMPELVIRFGAMPVSKPLFQLLKQNPSIRQIVVDPDGG
WRDPTQLSAHMLHCTEEEIAVLADSAVNSENRTGNWLEKWRFANSRFRALLRNLEAEDLTFEGNIYRNLQHLIPDNSSVF
VGNSMPIRDADTFFENQERTFRIFANRGANGIDGVVSTALGIYAATKEPVTLVIGDLSFYHDLNGLLVSKTLDIPLTVVL
VNNDGGGIFSFLPQSAEERYFELLFGTPTGLEFEHAAKLYGGRYICPESSAHFADAYLSSVNRRGLDIIEVKTKRDDRVE
QHRRMLDSALHEIRKEWPF

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=579, Percent_Identity=31.0880829015544, Blast_Score=230, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_BACLD (Q65FT2)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080359.1
- RefSeq:   YP_092775.1
- ProteinModelPortal:   Q65FT2
- STRING:   Q65FT2
- EnsemblBacteria:   EBBACT00000057581
- EnsemblBacteria:   EBBACT00000060953
- GeneID:   3027969
- GeneID:   3099133
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03222
- KEGG:   bli:BL02408
- NMPDR:   fig|279010.5.peg.145
- eggNOG:   COG1165
- GeneTree:   EBGT00050000000626
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- BioCyc:   BLIC279010-1:BLI03222-MONOMER
- BioCyc:   BLIC279010:BL02408-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 64655; Mature: 64524

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENPITKYIGNFIDELSLAGVTEAVICPGSRSTPLAILAASHPDINVHIHVDERSAAFF
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCEEEEEEECCCCHHHH
AMGMAKAMRKPVALICTSGTAAANFYPAVIEAHYSRIPLMILTADRPHELREVGAPQAID
HHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHHHH
QQFLFGNFVKFFTDSALPDSHPDMLAYIRSLAARAAGEAKKAPRGPVHINFPLREPLMPN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCC
LEDEPFQRLRSGSHVTVESGRFRADRHELVALSALIKETEKGVIVCGELQDDDAKEHIVA
CCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
LSQAAEYPILADPLSNLRNGTHSKETIIDAYDSLLKEEQLKRELMPELVIRFGAMPVSKP
HHHHCCCCEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
LFQLLKQNPSIRQIVVDPDGGWRDPTQLSAHMLHCTEEEIAVLADSAVNSENRTGNWLEK
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHH
WRFANSRFRALLRNLEAEDLTFEGNIYRNLQHLIPDNSSVFVGNSMPIRDADTFFENQER
HHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHCCCCC
TFRIFANRGANGIDGVVSTALGIYAATKEPVTLVIGDLSFYHDLNGLLVSKTLDIPLTVV
EEEEEECCCCCCHHHHHHHHHHHHEECCCCEEEEEECHHHHHCCCCEEEEEECCCCEEEE
LVNNDGGGIFSFLPQSAEERYFELLFGTPTGLEFEHAAKLYGGRYICPESSAHFADAYLS
EEECCCCCCHHHCCCHHHHHHHHHEECCCCCCCHHHHHHHHCCEEECCCCCCHHHHHHHH
SVNRRGLDIIEVKTKRDDRVEQHRRMLDSALHEIRKEWPF
HHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TENPITKYIGNFIDELSLAGVTEAVICPGSRSTPLAILAASHPDINVHIHVDERSAAFF
CCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCEEEEEEECCCCHHHH
AMGMAKAMRKPVALICTSGTAAANFYPAVIEAHYSRIPLMILTADRPHELREVGAPQAID
HHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHHHH
QQFLFGNFVKFFTDSALPDSHPDMLAYIRSLAARAAGEAKKAPRGPVHINFPLREPLMPN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCC
LEDEPFQRLRSGSHVTVESGRFRADRHELVALSALIKETEKGVIVCGELQDDDAKEHIVA
CCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
LSQAAEYPILADPLSNLRNGTHSKETIIDAYDSLLKEEQLKRELMPELVIRFGAMPVSKP
HHHHCCCCEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
LFQLLKQNPSIRQIVVDPDGGWRDPTQLSAHMLHCTEEEIAVLADSAVNSENRTGNWLEK
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHH
WRFANSRFRALLRNLEAEDLTFEGNIYRNLQHLIPDNSSVFVGNSMPIRDADTFFENQER
HHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHCCCCC
TFRIFANRGANGIDGVVSTALGIYAATKEPVTLVIGDLSFYHDLNGLLVSKTLDIPLTVV
EEEEEECCCCCCHHHHHHHHHHHHEECCCCEEEEEECHHHHHCCCCEEEEEECCCCEEEE
LVNNDGGGIFSFLPQSAEERYFELLFGTPTGLEFEHAAKLYGGRYICPESSAHFADAYLS
EEECCCCCCHHHCCCHHHHHHHHHEECCCCCCCHHHHHHHHCCEEECCCCCCHHHHHHHH
SVNRRGLDIIEVKTKRDDRVEQHRRMLDSALHEIRKEWPF
HHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA